High-performance liquid chromatographic approach to the separation of antiviral and immunostimulant fractions in Neuramide
Identifieur interne : 002087 ( Main/Exploration ); précédent : 002086; suivant : 002088High-performance liquid chromatographic approach to the separation of antiviral and immunostimulant fractions in Neuramide
Auteurs : Niccol Miraglia [Italie] ; Bruno Rindone [Italie] ; Giancarlo Folchito [Italie] ; Paola Amicucci [Italie] ; Guido Antonelli [Italie] ; Arnalda Lanfranchi [Italie] ; Margherita Massa [Italie]Source :
- Journal of Chromatography A [ 0021-9673 ] ; 1990.
English descriptors
- Teeft :
- Active fraction, Active material, Ammonium acetate buffer, Antiviral, Antiviral action, Antiviral activity, Antiviral components, Antiviral fraction, Assay, Biological activity, Chromatographic fractions, Difa cooper, Elemental analysis, Foetal calf serum, Herpes viruses, Hplc, Human lymphocyte proliferation, Immunostimulant, Immunostimulant activity, Immunostimulant fractions, Immunostimulating, Immunostimulating activity, Influenza, Influenza virus, Lymphocyte, Methylene chloride, Much sodium chloride, Neuramide, Palo alto, Phytohaemagglutinin, Sodium chloride, Sodium chloride solution, Tunable absorbance detector, Ultrafiltered, Ultrafiltered fraction, Waters assoc.
Abstract
Abstract: Neuramide, a tissue extract having antiviral action against influenza A virus and immunostimulant action, was analyzed by preparative size-exclusion high-performance liquid chromatography (HPLC) and a low-molecular-weight fraction responsible for the antiviral action was isolated after reversed-phase HPLC. Four fractions having immunostimulant activity were also isolated, as evidenced by their potentiating action in the human lymphocyte proliferation induced by phytohaemagglutinin.
Url:
DOI: 10.1016/S0021-9673(01)89478-4
Affiliations:
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