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Sequence and crystallization of influenza virus b/Beijing/1/87 neuraminidase

Identifieur interne : 002011 ( Main/Exploration ); précédent : 002010; suivant : 002012

Sequence and crystallization of influenza virus b/Beijing/1/87 neuraminidase

Auteurs : Wilhelm-Pascal Burmeister [France] ; Rod S. Daniels [Royaume-Uni, France] ; Sonia Dayan [France] ; Jean Gagnonj [Royaume-Uni, France] ; Stephen Cusack [France] ; Rob W. H. Ruigrok [France]

Source :

RBID : ISTEX:423F0BC064563F78D01008534D9D84AEF242CAFA

English descriptors

Abstract

Abstract: Influenza B/Beijing/1/87 neuraminidase heads were isolated from virus via trypsin digestion and characterized by PAGE, N-terminal sequencing, electron microscopy, and enzyme activity. The heads were crystallized into two crystal forms; tetragonal plates, like other neuraminidase crystals described before, that diffract to medium resolution (3 A) and a new form consisting of trigonal prisms or needles that diffract to high resolution (at least 2 A). The gene segment coding for neuraminidase was sequenced and compared with the neuraminidase sequence of B/Lee/40. The deduced amino acid sequences for neuraminidase showed only a 7% difference, whereas those for the NB proteins differed by 20%.

Url:
DOI: 10.1016/0042-6822(91)90031-6


Affiliations:


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Le document en format XML

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<div type="abstract" xml:lang="en">Abstract: Influenza B/Beijing/1/87 neuraminidase heads were isolated from virus via trypsin digestion and characterized by PAGE, N-terminal sequencing, electron microscopy, and enzyme activity. The heads were crystallized into two crystal forms; tetragonal plates, like other neuraminidase crystals described before, that diffract to medium resolution (3 A) and a new form consisting of trigonal prisms or needles that diffract to high resolution (at least 2 A). The gene segment coding for neuraminidase was sequenced and compared with the neuraminidase sequence of B/Lee/40. The deduced amino acid sequences for neuraminidase showed only a 7% difference, whereas those for the NB proteins differed by 20%.</div>
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