H2N2V1, Main, Exploration, bibRecord, 001F34

Synthetic recombinant vaccine expressing influenza haemagglutinin epitope in Salmonella flagellin leads to partial protection in mice

Identifieur interne : 001F34 ( Main/Exploration ); précédent : 001F33; suivant : 001F35

Synthetic recombinant vaccine expressing influenza haemagglutinin epitope in Salmonella flagellin leads to partial protection in mice

Auteurs : Juan Mcewen [Israël] ; Raphael Levi [Israël] ; Robert J. Horwitz [Israël] ; Ruth Arnon [Israël]

Source :

Vaccine [ 0264-410X ] ; 1992.

RBID : ISTEX:5625D8D88B44E1CE7A20A1F50C0C58636C46A217

English descriptors

Teeft :
- Adjuvant, Alkaline phosphatase, Allantoic, Allantoic cavity, Allantoic fluid, Animal model, Assay, Challenge infection, Chimeric flagellin, Cholera toxin, Cholera toxin epitope, Codon usage, Competent cells, Elisa, Embryonated eggs, Epitope, First report, Flagellin, Flagellin gene, Fusion protein, Haemagglutinin, Heterologous antigens, Homogenate, Humoral, Humoral response, Hybrid flagellin, Immune, Immune response, Immune system, Immunization, Influenza, Influenza subtype, Influenza vaccine, Influenza virus, Intact virus, Intranasal, Intranasal administration, Intranasal immunization, Intranasal route, Lung homogenates, Lung washes, Mice strains, Molecular weight, Natl acad, Oligonucleotides, Oligonucleotides coding, Oral administration, Oral immunization, Partial protection, Peptide, Plasmid, Positive response, Present study, Protective effect, Protective immunity, Recent studies, Reciprocal serum, Recombinant, Recombinant bacteria, Recombinant flagellin, Recombinant plasmid, Salmonella, Salmonella flagellin, Salmonella typhimurium, Second method, Serum antibody, Shiga toxin, Successful results, Such proteins, Synthetic oligonucleotide, Synthetic peptide, Synthetic peptides, Toxin, Typhimurium, Untreated control, Vaccine, Vaccine strain, Viral, Viral challenge, Virus presence, Weizmann institute, Whole bacteria, Wrong orientation.

Abstract

Abstract: The influenza virus haemagglutinin epitope 91–108, which is a conserved amino acid sequence in all type A H3 strains, was expressed in Salmonella flagellin, to evaluate its potential as a vaccine. For that purpose, a synthetic oligonucleotide comprising 54 bases coding for the corresponding sequence was inserted into the plasmid pLS408 and transformed into Escherichia coli JM101. Colonies containing the recombinant plasmid were used to transform Salmonella typhimurium LB5000 and were then transduced to a flagellin negative ‘live vaccine’ aroA mutant of Salmonella dublin. Rabbits immunized either with the live recombinant S. dublin or with the flagellin isolated from it, showed significant levels of IgG response against the synthetic peptide 91–108 as well as against the intact A/Texas/77 influenza virus. Mice immunized with the same preparations developed influenza-specific IgG antibodies in the blood and secreted IgA antibodies in their lungs. Furthermore, these mice showed about 50% protection against challenge infection with the virus. The most successful results were achieved by intranasal immunization with the isolated recombinant flagellin, when employed without the aid of adjuvant.

Url:

https://api.istex.fr/ark:/67375/6H6-J5PF4MK6-P/fulltext.pdf

DOI: 10.1016/0264-410X(92)90071-Q

Affiliations:

Israël

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Le document en format XML

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<term>Alkaline phosphatase</term>
<term>Allantoic</term>
<term>Allantoic cavity</term>
<term>Allantoic fluid</term>
<term>Animal model</term>
<term>Assay</term>
<term>Challenge infection</term>
<term>Chimeric flagellin</term>
<term>Cholera toxin</term>
<term>Cholera toxin epitope</term>
<term>Codon usage</term>
<term>Competent cells</term>
<term>Elisa</term>
<term>Embryonated eggs</term>
<term>Epitope</term>
<term>First report</term>
<term>Flagellin</term>
<term>Flagellin gene</term>
<term>Fusion protein</term>
<term>Haemagglutinin</term>
<term>Heterologous antigens</term>
<term>Homogenate</term>
<term>Humoral</term>
<term>Humoral response</term>
<term>Hybrid flagellin</term>
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<term>Immune response</term>
<term>Immune system</term>
<term>Immunization</term>
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<term>Intranasal administration</term>
<term>Intranasal immunization</term>
<term>Intranasal route</term>
<term>Lung homogenates</term>
<term>Lung washes</term>
<term>Mice strains</term>
<term>Molecular weight</term>
<term>Natl acad</term>
<term>Oligonucleotides</term>
<term>Oligonucleotides coding</term>
<term>Oral administration</term>
<term>Oral immunization</term>
<term>Partial protection</term>
<term>Peptide</term>
<term>Plasmid</term>
<term>Positive response</term>
<term>Present study</term>
<term>Protective effect</term>
<term>Protective immunity</term>
<term>Recent studies</term>
<term>Reciprocal serum</term>
<term>Recombinant</term>
<term>Recombinant bacteria</term>
<term>Recombinant flagellin</term>
<term>Recombinant plasmid</term>
<term>Salmonella</term>
<term>Salmonella flagellin</term>
<term>Salmonella typhimurium</term>
<term>Second method</term>
<term>Serum antibody</term>
<term>Shiga toxin</term>
<term>Successful results</term>
<term>Such proteins</term>
<term>Synthetic oligonucleotide</term>
<term>Synthetic peptide</term>
<term>Synthetic peptides</term>
<term>Toxin</term>
<term>Typhimurium</term>
<term>Untreated control</term>
<term>Vaccine</term>
<term>Vaccine strain</term>
<term>Viral</term>
<term>Viral challenge</term>
<term>Virus presence</term>
<term>Weizmann institute</term>
<term>Whole bacteria</term>
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<front><div type="abstract" xml:lang="en">Abstract: The influenza virus haemagglutinin epitope 91–108, which is a conserved amino acid sequence in all type A H3 strains, was expressed in Salmonella flagellin, to evaluate its potential as a vaccine. For that purpose, a synthetic oligonucleotide comprising 54 bases coding for the corresponding sequence was inserted into the plasmid pLS408 and transformed into Escherichia coli JM101. Colonies containing the recombinant plasmid were used to transform Salmonella typhimurium LB5000 and were then transduced to a flagellin negative ‘live vaccine’ aroA mutant of Salmonella dublin. Rabbits immunized either with the live recombinant S. dublin or with the flagellin isolated from it, showed significant levels of IgG response against the synthetic peptide 91–108 as well as against the intact A/Texas/77 influenza virus. Mice immunized with the same preparations developed influenza-specific IgG antibodies in the blood and secreted IgA antibodies in their lungs. Furthermore, these mice showed about 50% protection against challenge infection with the virus. The most successful results were achieved by intranasal immunization with the isolated recombinant flagellin, when employed without the aid of adjuvant.</div>
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Synthetic recombinant vaccine expressing influenza haemagglutinin epitope in Salmonella flagellin leads to partial protection in mice

Synthetic recombinant vaccine expressing influenza haemagglutinin epitope in Salmonella flagellin leads to partial protection in mice

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri