A single amino acid in the PB2 gene of influenza A virus is a determinant of host range.
Identifieur interne : 001E56 ( Main/Exploration ); précédent : 001E55; suivant : 001E57A single amino acid in the PB2 gene of influenza A virus is a determinant of host range.
Auteurs : E K Subbarao ; W. London ; B R MurphySource :
- Journal of Virology [ 0022-538X ] ; 1993.
Abstract
The single gene reassortant virus that derives its PB2 gene from the avian influenza A/Mallard/NY/78 virus and remaining genes from the human influenza A/Los Angeles/2/87 virus exhibits a host range restriction (hr) phenotype characterized by efficient replication in avian tissue and failure to produce plaques in mammalian Madin-Darby canine kidney cells. The hr phenotype is associated with restriction of viral replication in the respiratory tract of squirrel monkeys and humans. To identify the genetic basis of the hr phenotype, we isolated four phenotypic hr mutant viruses that acquired the ability to replicate efficiently in mammalian tissue. Segregational analysis indicated that the loss of the hr phenotype was due to a mutation in the PB2 gene itself. The nucleotide sequences of the PB2 gene of each of the four hr mutants revealed that a single amino acid substitution at position 627 (Glu-->Lys) was responsible for the restoration of the ability of the PB2 single gene reassortant to replicate in Madin-Darby canine kidney cells. Interestingly, the amino acid at position 627 in every avian influenza A virus PB2 protein analyzed to date is glutamic acid, and in every human influenza A virus PB2 protein, it is lysine. Thus, the amino acid at residue 627 of PB2 is an important determinant of host range of influenza A viruses.
Url:
PubMed: 8445709
PubMed Central: 240216
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000416
- to stream Pmc, to step Curation: 000416
- to stream Pmc, to step Checkpoint: 000D08
- to stream Ncbi, to step Merge: 001323
- to stream Ncbi, to step Curation: 001323
- to stream Ncbi, to step Checkpoint: 001323
- to stream Main, to step Merge: 001F44
- to stream Main, to step Curation: 001E56
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A single amino acid in the PB2 gene of influenza A virus is a determinant of host range.</title>
<author><name sortKey="Subbarao, E K" sort="Subbarao, E K" uniqKey="Subbarao E" first="E K" last="Subbarao">E K Subbarao</name>
</author>
<author><name sortKey="London, W" sort="London, W" uniqKey="London W" first="W" last="London">W. London</name>
</author>
<author><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">8445709</idno>
<idno type="pmc">240216</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC240216</idno>
<idno type="RBID">PMC:240216</idno>
<date when="1993">1993</date>
<idno type="wicri:Area/Pmc/Corpus">000416</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000416</idno>
<idno type="wicri:Area/Pmc/Curation">000416</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000416</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000D08</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000D08</idno>
<idno type="wicri:Area/Ncbi/Merge">001323</idno>
<idno type="wicri:Area/Ncbi/Curation">001323</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001323</idno>
<idno type="wicri:doubleKey">0022-538X:1993:Subbarao E:a:single:amino</idno>
<idno type="wicri:Area/Main/Merge">001F44</idno>
<idno type="wicri:Area/Main/Curation">001E56</idno>
<idno type="wicri:Area/Main/Exploration">001E56</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A single amino acid in the PB2 gene of influenza A virus is a determinant of host range.</title>
<author><name sortKey="Subbarao, E K" sort="Subbarao, E K" uniqKey="Subbarao E" first="E K" last="Subbarao">E K Subbarao</name>
</author>
<author><name sortKey="London, W" sort="London, W" uniqKey="London W" first="W" last="London">W. London</name>
</author>
<author><name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="1993">1993</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The single gene reassortant virus that derives its PB2 gene from the avian influenza A/Mallard/NY/78 virus and remaining genes from the human influenza A/Los Angeles/2/87 virus exhibits a host range restriction (hr) phenotype characterized by efficient replication in avian tissue and failure to produce plaques in mammalian Madin-Darby canine kidney cells. The hr phenotype is associated with restriction of viral replication in the respiratory tract of squirrel monkeys and humans. To identify the genetic basis of the hr phenotype, we isolated four phenotypic hr mutant viruses that acquired the ability to replicate efficiently in mammalian tissue. Segregational analysis indicated that the loss of the hr phenotype was due to a mutation in the PB2 gene itself. The nucleotide sequences of the PB2 gene of each of the four hr mutants revealed that a single amino acid substitution at position 627 (Glu-->Lys) was responsible for the restoration of the ability of the PB2 single gene reassortant to replicate in Madin-Darby canine kidney cells. Interestingly, the amino acid at position 627 in every avian influenza A virus PB2 protein analyzed to date is glutamic acid, and in every human influenza A virus PB2 protein, it is lysine. Thus, the amino acid at residue 627 of PB2 is an important determinant of host range of influenza A viruses.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="London, W" sort="London, W" uniqKey="London W" first="W" last="London">W. London</name>
<name sortKey="Murphy, B R" sort="Murphy, B R" uniqKey="Murphy B" first="B R" last="Murphy">B R Murphy</name>
<name sortKey="Subbarao, E K" sort="Subbarao, E K" uniqKey="Subbarao E" first="E K" last="Subbarao">E K Subbarao</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E56 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001E56 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:240216 |texte= A single amino acid in the PB2 gene of influenza A virus is a determinant of host range. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:8445709" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a H2N2V1
This area was generated with Dilib version V0.6.33. |