H2N2V1, Main, Exploration, bibRecord, 001C78

Characterization of a Hemagglutinin-Specific Inhibitor of Influenza A Virus

Identifieur interne : 001C78 ( Main/Exploration ); précédent : 001C77; suivant : 001C79

Characterization of a Hemagglutinin-Specific Inhibitor of Influenza A Virus

Auteurs : Guangxiang Luo ; Richard Colonno ; Mark Krystal

Source :

Virology [ 0042-6822 ] ; 1996.

RBID : ISTEX:9B02FA72572076657E9D3AB772FFE4C85D038DB0

English descriptors

Teeft :
- Acid change, Agents chemother, Amino, Amino acid change, Antimicrob, Antiviral, Antiviral activity, Assay, Blood cells, Cdna clones, Channel activity, Chemother, Clone, Coding strand, Colonno, Conformational change, Days incubation, Drug resistance, Early stage, Enami, Endosomal membranes, Fusion process, Genome, Hayden, Hemagglutinin, Hemagglutinin protein, Hemolysis, Herpes simplex virus, Influenza, Influenza hemagglutinin, Influenza virus, Influenza viruses, Inhibitor, Inhibitory activity, Krystal, Mdbk, Mdbk cells, Mdck, Mdck cells, Membrane fusion, Multicycle, Mutation, Neuraminidase, Palese, Plaque, Plaque assay, Plenum press, Polymerase, Reassortant, Reassortant virus, Receptor, Receptor binding, Replication, Resistant, Resistant phenotype, Resistant virus, Sialic acid, Skehel, Small molecule inhibitors, Subtype, Subtype viruses, Subtypes, Subunit, Tissue culture, Transfectant, Transfectant virus, Transfectant viruses, Various amounts, Various concentrations, Vesicular stomatitis virus, Viral, Viras, Virol, Virology, Virology inhibitor, Virus, Virus growth, Virus infection.

Abstract

Abstract: A novel inhibitor of influenza virus growth in tissue culture was identified and characterized. This compound (BMY-27709) has an IC50of 3–8 μMfor A/WSN/33 virus growth in a multicycle replication assay and is active against all H1 and H2 subtype viruses tested. However, BMY-27709 was found to be inactive against H3 subtype viruses, as well as influenza B/Lee/40 virus. BMY-27709 was also found to act against H1 and H2 viruses early in infection, suggesting that the target for inhibition is the hemagglutinin protein. This was confirmed through the use of reassortant viruses and the isolation of a virus resistant to BMY-27709. The resistant virus isolated contained a phenylalanine to serine change at amino acid 110 of the HA2 subunit. That this single mutation was responsible for the acquisition of resistance to BMY-27709 was proven through reverse genetics, as transfectant virus containing only this change was shown to be resistant to BMY-27709, while the control virus without this mutation remained sensitive. BMY-27709 is able to inhibit virus-induced red blood cell hemolysis, suggesting that it blocks the membrane fusion function of hemagglutinin. These experiments further illustrate that the hemagglutinin protein of influenza virus is a viable target for the discovery and development of small molecule inhibitors of virus growth.

Url:

https://api.istex.fr/ark:/67375/6H6-F0WCBDFR-X/fulltext.pdf

DOI: 10.1006/viro.1996.0628

Affiliations:

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Le document en format XML

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<term>Antiviral activity</term>
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<front><div type="abstract" xml:lang="en">Abstract: A novel inhibitor of influenza virus growth in tissue culture was identified and characterized. This compound (BMY-27709) has an IC50of 3–8 μMfor A/WSN/33 virus growth in a multicycle replication assay and is active against all H1 and H2 subtype viruses tested. However, BMY-27709 was found to be inactive against H3 subtype viruses, as well as influenza B/Lee/40 virus. BMY-27709 was also found to act against H1 and H2 viruses early in infection, suggesting that the target for inhibition is the hemagglutinin protein. This was confirmed through the use of reassortant viruses and the isolation of a virus resistant to BMY-27709. The resistant virus isolated contained a phenylalanine to serine change at amino acid 110 of the HA2 subunit. That this single mutation was responsible for the acquisition of resistance to BMY-27709 was proven through reverse genetics, as transfectant virus containing only this change was shown to be resistant to BMY-27709, while the control virus without this mutation remained sensitive. BMY-27709 is able to inhibit virus-induced red blood cell hemolysis, suggesting that it blocks the membrane fusion function of hemagglutinin. These experiments further illustrate that the hemagglutinin protein of influenza virus is a viable target for the discovery and development of small molecule inhibitors of virus growth.</div>
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Characterization of a Hemagglutinin-Specific Inhibitor of Influenza A Virus

Characterization of a Hemagglutinin-Specific Inhibitor of Influenza A Virus

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri