Immunization of pigs against influenza virus infection by DNA vaccine priming followed by killed-virus vaccine boosting
Identifieur interne : 001873 ( Main/Exploration ); précédent : 001872; suivant : 001874Immunization of pigs against influenza virus infection by DNA vaccine priming followed by killed-virus vaccine boosting
Auteurs : Diane L. Larsen [États-Unis] ; Alexander Karasin [États-Unis] ; Christopher W. Olsen [États-Unis]Source :
- Vaccine [ 0264-410X ] ; 2001.
Descripteurs français
- Wicri :
- topic : Porcin.
English descriptors
- KwdEn :
- Teeft :
- Alternative approach, Antibody responses, Antibody titers, Antigenic drift, Arch intern, Assay, Average number, Booster vaccinations, Cell biol, Cellular responses, Challenge infection, Challenge virus, Clin microbiol, Clinical disease, Cohort, Common symbol, Control pigs, Conventional vaccine, Data points, Detection limits, Donnelly, Dos, Elisa, Elisa assays, Elisa titers, Elispot, Elispot assay, Elispot assays, Elsevier science, Embryonated chicken eggs, Equal volume, Experimental infection, Experimental pigs, Fatal case, Genetic reassortment, Hemagglutinin, High serum, Higher numbers, Higher serum, Highest dilution, Human virus type, Immune, Immune response, Immune responses, Immunization, Immunol, Immunol immunopath, Infection, Infection control, Intramuscular injection, Intranasal challenge, Intratracheal challenge, Larsen, Less virus, Lymph nodes, Microbiol, Mononuclear, Mononuclear cells, Mouse model, Mucosal, Nasal, Nasal mucosa, Nasal passages, Nasal secretion samples, Nasal secretions, Nasal wash samples, Nasal washes, Negative control, Negative control sample, Negative control wells, Optical density, Other cohorts, Pig, Pigs euthanized, Pigs vaccinated, Porcine, Porcine ifng, Positive control, Post challenge samples, Prechallenge, Prechallenge samples, Present study, Previous results, Previous studies, Previous study, Proc, Proc natl acad, Protective immunity, Recombinant, Room temperature, Sample wells, Second vaccination, Second vaccinations, Serial dilutions, Serum antibodies, Serum antibody responses, Spleen, Swine, Swine virus, Swine viruses, Titer, Tracheobronchial lymph nodes, Uninfected control pigs, Upper airways, Vaccinated, Vaccinated pigs, Vaccination, Vaccine, Vaccine cohorts, Vaccinia virus ankara, Viral, Viral antigen, Viral protein, Virol, Virus, Virus challenge, Virus clearance, Virus hemagglutinin, Virus infection, Virus infections, Virus titers, Virus vaccine, Wash solution, Whole blood, Whole virus vaccine.
Abstract
Abstract: In a previous study of particle-mediated DNA vaccination of pigs, it was found that administration of an influenza virus hemagglutinin (HA) gene elicited low levels of virus-specific antibody, but did not provide significant protection from challenge infection (as evidenced by virus shedding in nasal secretions). However, the vaccinated pigs developed high antibody titers after exposure to the challenge virus, suggesting strong priming of humoral immune responses by DNA vaccination. In the present study, pigs given a conventional, inactivated influenza virus vaccine 4 weeks after a priming dose of HA DNA developed higher levels of virus-specific serum antibodies and were protected from challenge virus infection to a significantly greater degree than pigs that received two doses of DNA vaccine.
Url:
DOI: 10.1016/S0264-410X(01)00014-7
Affiliations:
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science</term><term>Embryonated chicken eggs</term><term>Equal volume</term><term>Experimental infection</term><term>Experimental pigs</term><term>Fatal case</term><term>Genetic reassortment</term><term>Hemagglutinin</term><term>High serum</term><term>Higher numbers</term><term>Higher serum</term><term>Highest dilution</term><term>Human virus type</term><term>Immune</term><term>Immune response</term><term>Immune responses</term><term>Immunization</term><term>Immunol</term><term>Immunol immunopath</term><term>Infection</term><term>Infection control</term><term>Intramuscular injection</term><term>Intranasal challenge</term><term>Intratracheal challenge</term><term>Larsen</term><term>Less virus</term><term>Lymph nodes</term><term>Microbiol</term><term>Mononuclear</term><term>Mononuclear cells</term><term>Mouse model</term><term>Mucosal</term><term>Nasal</term><term>Nasal mucosa</term><term>Nasal passages</term><term>Nasal secretion samples</term><term>Nasal secretions</term><term>Nasal wash samples</term><term>Nasal washes</term><term>Negative control</term><term>Negative control sample</term><term>Negative control wells</term><term>Optical density</term><term>Other cohorts</term><term>Pig</term><term>Pigs euthanized</term><term>Pigs vaccinated</term><term>Porcine</term><term>Porcine ifng</term><term>Positive control</term><term>Post challenge samples</term><term>Prechallenge</term><term>Prechallenge samples</term><term>Present study</term><term>Previous results</term><term>Previous studies</term><term>Previous study</term><term>Proc</term><term>Proc natl acad</term><term>Protective immunity</term><term>Recombinant</term><term>Room temperature</term><term>Sample wells</term><term>Second vaccination</term><term>Second vaccinations</term><term>Serial dilutions</term><term>Serum antibodies</term><term>Serum antibody responses</term><term>Spleen</term><term>Swine</term><term>Swine virus</term><term>Swine viruses</term><term>Titer</term><term>Tracheobronchial lymph nodes</term><term>Uninfected control pigs</term><term>Upper airways</term><term>Vaccinated</term><term>Vaccinated pigs</term><term>Vaccination</term><term>Vaccine</term><term>Vaccine cohorts</term><term>Vaccinia virus ankara</term><term>Viral</term><term>Viral antigen</term><term>Viral protein</term><term>Virol</term><term>Virus</term><term>Virus challenge</term><term>Virus clearance</term><term>Virus hemagglutinin</term><term>Virus infection</term><term>Virus infections</term><term>Virus titers</term><term>Virus vaccine</term><term>Wash solution</term><term>Whole blood</term><term>Whole virus vaccine</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Porcin</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In a previous study of particle-mediated DNA vaccination of pigs, it was found that administration of an influenza virus hemagglutinin (HA) gene elicited low levels of virus-specific antibody, but did not provide significant protection from challenge infection (as evidenced by virus shedding in nasal secretions). However, the vaccinated pigs developed high antibody titers after exposure to the challenge virus, suggesting strong priming of humoral immune responses by DNA vaccination. In the present study, pigs given a conventional, inactivated influenza virus vaccine 4 weeks after a priming dose of HA DNA developed higher levels of virus-specific serum antibodies and were protected from challenge virus infection to a significantly greater degree than pigs that received two doses of DNA vaccine.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Wisconsin</li></region></list><tree><country name="États-Unis"><region name="Wisconsin"><name sortKey="Larsen, Diane L" sort="Larsen, Diane L" uniqKey="Larsen D" first="Diane L." last="Larsen">Diane L. Larsen</name></region><name sortKey="Karasin, Alexander" sort="Karasin, Alexander" uniqKey="Karasin A" first="Alexander" last="Karasin">Alexander Karasin</name><name sortKey="Olsen, Christopher W" sort="Olsen, Christopher W" uniqKey="Olsen C" first="Christopher W." last="Olsen">Christopher W. Olsen</name><name sortKey="Olsen, Christopher W" sort="Olsen, Christopher W" uniqKey="Olsen C" first="Christopher W." last="Olsen">Christopher W. Olsen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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