H2N2V1, Main, Exploration, bibRecord, 001385

Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray▿ †

Identifieur interne : 001385 ( Main/Exploration ); précédent : 001384; suivant : 001386

Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray▿ †

Auteurs : Phenix-Lan Quan ; Gustavo Palacios ; Omar J. Jabado ; Sean Conlan ; David L. Hirschberg ; Francisco Pozo ; Philippa J. M. Jack ; Daniel Cisterna ; Neil Renwick ; Jeffrey Hui ; Andrew Drysdale ; Rachel Amos-Ritchie ; Elsa Baumeister ; Vilma Savy ; Kelly M. Lager ; Jürgen A. Richt ; David B. Boyle ; Adolfo García-Sastre ; Inmaculada Casas ; Pilar Perez-Bre A ; Thomas Briese ; W. Ian Lipkin

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2007.

RBID : PMC:1951265

Abstract

Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1951265

DOI: 10.1128/JCM.00737-07
PubMed: 17553978
PubMed Central: 1951265

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.</p>
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Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray▿ †

Detection of Respiratory Viruses and Subtype Identification of Influenza A Viruses by GreeneChipResp Oligonucleotide Microarray▿ †

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Abstract

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