H2N2V1, Main, Exploration, bibRecord, 000F61

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

Identifieur interne : 000F61 ( Main/Exploration ); précédent : 000F60; suivant : 000F62

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

Auteurs : Alexander Nagy [République tchèque] ; Veronika Vostinakova [République tchèque] ; Zuzana Pirchanova [République tchèque] ; Lenka Cernikova [République tchèque] ; Zuzana Dirbakova ; Miroslav Mojzis ; Helena Jirincova [République tchèque] ; Martina Havlickova [République tchèque] ; Adam Dan [Hongrie] ; Krisztina Ursu [Hongrie] ; Stefan Vilcek ; Jitka Hornickova [République tchèque]

Source :

Archives of Virology [ 0304-8608 ] ; 2010-05-01.

RBID : ISTEX:CB716526A840C9EA4BD11F4F8DAC088498FF6DED

Abstract

Abstract: The objective of our study was to develop and evaluate a TaqMan real-time RT-PCR (RRT-PCR) assay for universal detection of influenza A (IA) viruses. The primers and LNA-modified octanucleotide probe were selected to correspond to extremely conserved regions of the membrane protein (MP) segment identified by a comprehensive bioinformatics analysis including 10,405 IA viruses MP sequences, i.e., all of the sequences of the Influenza Virus Sequence database collected as of August 20, 2009. The RRT-PCR has a detection limit of approximately five copies of target RNA/reaction and excellent reaction parameters tested in four IA viruses reference laboratories. The inclusivity of the assay was estimated at both the bioinformatic and the experimental level. Our results predicted that this RRT-PCR assay was able to detect 99.5% of known human IA virus strains, 99.84% of pandemic influenza A (H1N1) strains, 99.75% of avian strains, 98.89% of swine strains, 98.15% of equine strains, and 100% of influenza A viruses of other origin.

Url:

https://api.istex.fr/ark:/67375/VQC-QL5V2BFV-Q/fulltext.pdf

DOI: 10.1007/s00705-010-0636-x

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: The objective of our study was to develop and evaluate a TaqMan real-time RT-PCR (RRT-PCR) assay for universal detection of influenza A (IA) viruses. The primers and LNA-modified octanucleotide probe were selected to correspond to extremely conserved regions of the membrane protein (MP) segment identified by a comprehensive bioinformatics analysis including 10,405 IA viruses MP sequences, i.e., all of the sequences of the Influenza Virus Sequence database collected as of August 20, 2009. The RRT-PCR has a detection limit of approximately five copies of target RNA/reaction and excellent reaction parameters tested in four IA viruses reference laboratories. The inclusivity of the assay was estimated at both the bioinformatic and the experimental level. Our results predicted that this RRT-PCR assay was able to detect 99.5% of known human IA virus strains, 99.84% of pandemic influenza A (H1N1) strains, 99.75% of avian strains, 98.89% of swine strains, 98.15% of equine strains, and 100% of influenza A viruses of other origin.</div>
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Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

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Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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