H1N1/09 Influenza A Virus Infection of Immortalized First Trimester Human Trophoblast Cell Lines
Identifieur interne : 000B22 ( Main/Exploration ); précédent : 000B21; suivant : 000B23H1N1/09 Influenza A Virus Infection of Immortalized First Trimester Human Trophoblast Cell Lines
Auteurs : Shihoko Komine-Aizawa [Japon] ; Ai Suzaki [Japon] ; Quang D. Trinh [Japon] ; Yasuyuki Izumi [Japon] ; Toshikatsu Shibata [Japon] ; Kazumichi Kuroda [Japon] ; Satoshi Hayakawa [Japon]Source :
- American Journal of Reproductive Immunology [ 1046-7408 ] ; 2012-09.
English descriptors
- Teeft :
- American journal, Apoptosis, Apoptotic cells, Assay, Cdna synthesis, Cell lines, Cohort study, Culture media, Cytopathic effects, Detectable levels, Direct effects, Direct sequencing, Flow cytometry, Haemagglutination assay, Immunology, Intensive care unit, Intracellular localization, John wiley sons, John wiley sons influenza virus infects trophoblast, Mdck, Mdck cell line, Mdck cells, Monoclonal antibody, Nihon university school, Pandemic, Pandemic virus, Paraformaldehyde solution, Perinatal outcomes, Positive control, Postpartum women, Pregnancy outcomes, Pregnant women, Present study, Previous data, Receptor, Reprod immunol, Reproductive, Reproductive immunology, Room temperature, Sambucus nigra lectin, Second trimester, Synthetic ssdna, Trimester, Trimester trophoblast cell lines, Trophoblast, Trophoblast cell lines, Uorescence assay, Uorescence microscope keyence, Uorescent images, Vector laboratories, Viral, Viral particles, Viral release, Virus, Virus infection, Virus receptor, Virus replicates.
Abstract
Problem: Although high maternal mortality was reported in the most recent pandemic of swine‐origin influenza A H1N1/09 (H1N1/09), its direct effects on the feto‐placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1 influenza A (H1N1/09) virus. Method of study: The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins. Results: Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected. Conclusion: First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.
Url:
DOI: 10.1111/j.1600-0897.2012.01172.x
Affiliations:
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In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1 influenza A (H1N1/09) virus. Method of study: The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins. Results: Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected. Conclusion: First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Komine Izawa, Shihoko" sort="Komine Izawa, Shihoko" uniqKey="Komine Izawa S" first="Shihoko" last="Komine-Aizawa">Shihoko Komine-Aizawa</name></region><name sortKey="Hayakawa, Satoshi" sort="Hayakawa, Satoshi" uniqKey="Hayakawa S" first="Satoshi" last="Hayakawa">Satoshi Hayakawa</name><name sortKey="Izumi, Yasuyuki" sort="Izumi, Yasuyuki" uniqKey="Izumi Y" first="Yasuyuki" last="Izumi">Yasuyuki Izumi</name><name sortKey="Komine Izawa, Shihoko" sort="Komine Izawa, Shihoko" uniqKey="Komine Izawa S" first="Shihoko" last="Komine-Aizawa">Shihoko Komine-Aizawa</name><name sortKey="Kuroda, Kazumichi" sort="Kuroda, Kazumichi" uniqKey="Kuroda K" first="Kazumichi" last="Kuroda">Kazumichi Kuroda</name><name sortKey="Shibata, Toshikatsu" sort="Shibata, Toshikatsu" uniqKey="Shibata T" first="Toshikatsu" last="Shibata">Toshikatsu Shibata</name><name sortKey="Suzaki, Ai" sort="Suzaki, Ai" uniqKey="Suzaki A" first="Ai" last="Suzaki">Ai Suzaki</name><name sortKey="Trinh, Quang D" sort="Trinh, Quang D" uniqKey="Trinh Q" first="Quang D." last="Trinh">Quang D. 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