A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology
Identifieur interne : 000604 ( Main/Exploration ); précédent : 000603; suivant : 000605A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology
Auteurs : Joseph Bucukovski [États-Unis] ; Neus Latorre-Margalef [États-Unis] ; David E. Stallknecht [États-Unis] ; Benjamin L. Miller [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
Influenza serology has traditionally relied on techniques such as hemagglutination inhibition, microneutralization, and ELISA. These assays are complex, challenging to implement in a format allowing detection of several types of antibody-analyte interactions at once (multiplex), and troublesome to implement in the field. As an alternative, we have developed a hemagglutinin microarray on the Arrayed Imaging Reflectometry (AIR) platform. AIR provides sensitive, rapid, and label-free multiplex detection of targets in complex analyte samples such as serum. In preliminary work, we demonstrated the application of this array to the testing of human samples from a vaccine trial. Here, we report the application of an expanded label-free hemagglutinin microarray to the analysis of avian serum samples. Samples from influenza virus challenge experiments in mallards yielded strong, selective detection of antibodies to the challenge antigen in most cases. Samples acquired in the field from mallards were also analyzed, and compared with viral hemagglutinin inhibition and microneutralization assays. We find that the AIR hemagglutinin microarray can provide a simple and robust alternative to standard methods, offering substantially greater information density from a simple workflow.
Url:
DOI: 10.1371/journal.pone.0134484
PubMed: 26241048
PubMed Central: 4524619
Affiliations:
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Miller</name><affiliation wicri:level="2"><nlm:aff id="aff001"><addr-line>Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, University of Rochester, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff002"><addr-line>Department of Dermatology, University of Rochester, Rochester, New York, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Dermatology, University of Rochester, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Influenza serology has traditionally relied on techniques such as hemagglutination inhibition, microneutralization, and ELISA. These assays are complex, challenging to implement in a format allowing detection of several types of antibody-analyte interactions at once (multiplex), and troublesome to implement in the field. As an alternative, we have developed a hemagglutinin microarray on the Arrayed Imaging Reflectometry (AIR) platform. AIR provides sensitive, rapid, and label-free multiplex detection of targets in complex analyte samples such as serum. In preliminary work, we demonstrated the application of this array to the testing of human samples from a vaccine trial. Here, we report the application of an expanded label-free hemagglutinin microarray to the analysis of avian serum samples. Samples from influenza virus challenge experiments in mallards yielded strong, selective detection of antibodies to the challenge antigen in most cases. Samples acquired in the field from mallards were also analyzed, and compared with viral hemagglutinin inhibition and microneutralization assays. We find that the AIR hemagglutinin microarray can provide a simple and robust alternative to standard methods, offering substantially greater information density from a simple workflow.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Katz, Jm" uniqKey="Katz J">JM Katz</name></author><author><name sortKey="Hancock, K" uniqKey="Hancock K">K Hancock</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lebarbenchon, C" uniqKey="Lebarbenchon C">C Lebarbenchon</name></author><author><name sortKey="Brown, Jd" uniqKey="Brown J">JD Brown</name></author><author><name sortKey="Luttrell, Mp" uniqKey="Luttrell M">MP Luttrell</name></author><author><name sortKey="Stallknecht, De" uniqKey="Stallknecht D">DE Stallknecht</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mace, Cr" uniqKey="Mace C">CR Mace</name></author><author><name sortKey="Striemer, Cc" uniqKey="Striemer C">CC Striemer</name></author><author><name sortKey="Miller, Bl" uniqKey="Miller B">BL Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mace, Cr" uniqKey="Mace C">CR Mace</name></author><author><name sortKey="Striemer, Cc" uniqKey="Striemer C">CC Striemer</name></author><author><name sortKey="Miller, Bl" uniqKey="Miller B">BL Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mace, Cr" uniqKey="Mace C">CR Mace</name></author><author><name sortKey="Topham, Dj" uniqKey="Topham D">DJ Topham</name></author><author><name sortKey="Mosmann, Tr" uniqKey="Mosmann T">TR Mosmann</name></author><author><name sortKey="Quataert, S" uniqKey="Quataert S">S Quataert</name></author><author><name sortKey="Treanor, J" uniqKey="Treanor J">J Treanor</name></author><author><name sortKey="Miller, Bl" uniqKey="Miller B">BL 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name="États-Unis"><region name="État de New York"><name sortKey="Bucukovski, Joseph" sort="Bucukovski, Joseph" uniqKey="Bucukovski J" first="Joseph" last="Bucukovski">Joseph Bucukovski</name></region><name sortKey="Bucukovski, Joseph" sort="Bucukovski, Joseph" uniqKey="Bucukovski J" first="Joseph" last="Bucukovski">Joseph Bucukovski</name><name sortKey="Latorre Margalef, Neus" sort="Latorre Margalef, Neus" uniqKey="Latorre Margalef N" first="Neus" last="Latorre-Margalef">Neus Latorre-Margalef</name><name sortKey="Miller, Benjamin L" sort="Miller, Benjamin L" uniqKey="Miller B" first="Benjamin L." last="Miller">Benjamin L. Miller</name><name sortKey="Miller, Benjamin L" sort="Miller, Benjamin L" uniqKey="Miller B" first="Benjamin L." last="Miller">Benjamin L. Miller</name><name sortKey="Stallknecht, David E" sort="Stallknecht, David E" uniqKey="Stallknecht D" first="David E." last="Stallknecht">David E. Stallknecht</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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