Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine
Identifieur interne : 000324 ( Main/Exploration ); précédent : 000323; suivant : 000325Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine
Auteurs : Amy Lingel ; Brianna L. Bullard ; Eric A. WeaverSource :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
Mice were immunized with Adenovirus expressing the H1-con, H2-con, H3-con and H5-con HA consensus genes in combination (multivalent) and compared to mice immunized with the traditional 2010–2011 FluZone and FluMist seasonal vaccines. Immunized mice were challenged with 10–100 MLD50 of H1N1, H3N1, H3N2 and H5N1 influenza viruses. The traditional vaccines induced robust levels of HA inhibition (HI) titers, but failed to protect against five different heterologous lethal influenza challenges. Conversely, the multivalent consensus vaccine (1 × 1010 virus particles (vp)/mouse) induced protective HI titers of ≥40 against 8 of 10 influenza viruses that represent a wide degree of divergence within the HA subtypes and protected 100% of mice from 8 of 9 lethal heterologous influenza virus challenges. The vaccine protection was dose dependent, in general, and a dose as low as 5 × 107 vp/mouse still provided 100% survival against 7 of 9 lethal heterologous influenza challenges. These data indicate that very low doses of Adenovirus-vectored consensus vaccines induce superior levels of immunity against a wide divergence of influenza subtypes as compared to traditional vaccines. These doses are scalable and translatable to humans and may provide the foundation for complete and long-lasting anti-influenza immunity.
Url:
DOI: 10.1038/s41598-017-14891-y
PubMed: 29097763
PubMed Central: 5668234
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000197
- to stream Pmc, to step Curation: 000197
- to stream Pmc, to step Checkpoint: 000244
- to stream Ncbi, to step Merge: 000E28
- to stream Ncbi, to step Curation: 000E28
- to stream Ncbi, to step Checkpoint: 000E28
- to stream Main, to step Merge: 000324
- to stream Main, to step Curation: 000324
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine</title><author><name sortKey="Lingel, Amy" sort="Lingel, Amy" uniqKey="Lingel A" first="Amy" last="Lingel">Amy Lingel</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Bullard, Brianna L" sort="Bullard, Brianna L" uniqKey="Bullard B" first="Brianna L." last="Bullard">Brianna L. Bullard</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Weaver, Eric A" sort="Weaver, Eric A" uniqKey="Weaver E" first="Eric A." last="Weaver">Eric A. Weaver</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29097763</idno><idno type="pmc">5668234</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5668234</idno><idno type="RBID">PMC:5668234</idno><idno type="doi">10.1038/s41598-017-14891-y</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000197</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000197</idno><idno type="wicri:Area/Pmc/Curation">000197</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000197</idno><idno type="wicri:Area/Pmc/Checkpoint">000244</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000244</idno><idno type="wicri:Area/Ncbi/Merge">000E28</idno><idno type="wicri:Area/Ncbi/Curation">000E28</idno><idno type="wicri:Area/Ncbi/Checkpoint">000E28</idno><idno type="wicri:Area/Main/Merge">000324</idno><idno type="wicri:Area/Main/Curation">000324</idno><idno type="wicri:Area/Main/Exploration">000324</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine</title><author><name sortKey="Lingel, Amy" sort="Lingel, Amy" uniqKey="Lingel A" first="Amy" last="Lingel">Amy Lingel</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Bullard, Brianna L" sort="Bullard, Brianna L" uniqKey="Bullard B" first="Brianna L." last="Bullard">Brianna L. Bullard</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Weaver, Eric A" sort="Weaver, Eric A" uniqKey="Weaver E" first="Eric A." last="Weaver">Eric A. Weaver</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Mice were immunized with Adenovirus expressing the H1-con, H2-con, H3-con and H5-con HA consensus genes in combination (multivalent) and compared to mice immunized with the traditional 2010–2011 FluZone and FluMist seasonal vaccines. Immunized mice were challenged with 10–100 MLD<sub>50</sub> of H1N1, H3N1, H3N2 and H5N1 influenza viruses. The traditional vaccines induced robust levels of HA inhibition (HI) titers, but failed to protect against five different heterologous lethal influenza challenges. Conversely, the multivalent consensus vaccine (1 × 10<sup>10</sup> virus particles (vp)/mouse) induced protective HI titers of ≥40 against 8 of 10 influenza viruses that represent a wide degree of divergence within the HA subtypes and protected 100% of mice from 8 of 9 lethal heterologous influenza virus challenges. The vaccine protection was dose dependent, in general, and a dose as low as 5 × 10<sup>7</sup> vp/mouse still provided 100% survival against 7 of 9 lethal heterologous influenza challenges. These data indicate that very low doses of Adenovirus-vectored consensus vaccines induce superior levels of immunity against a wide divergence of influenza subtypes as compared to traditional vaccines. These doses are scalable and translatable to humans and may provide the foundation for complete and long-lasting anti-influenza immunity.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Reed, C" uniqKey="Reed C">C Reed</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Appiah, Gd" uniqKey="Appiah G">GD Appiah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Berlanda Scorza, F" uniqKey="Berlanda Scorza F">F Berlanda Scorza</name></author><author><name sortKey="Tsvetnitsky, V" uniqKey="Tsvetnitsky V">V Tsvetnitsky</name></author><author><name sortKey="Donnelly, Jj" uniqKey="Donnelly J">JJ Donnelly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Webby, Rj" uniqKey="Webby R">RJ Webby</name></author><author><name sortKey="Weaver, Ea" uniqKey="Weaver E">EA Weaver</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Kerkhove, Md" uniqKey="Van Kerkhove M">MD Van Kerkhove</name></author><author><name sortKey="Hirve, S" uniqKey="Hirve S">S Hirve</name></author><author><name sortKey="Koukounari, A" uniqKey="Koukounari A">A Koukounari</name></author><author><name sortKey="Mounts, Aw" uniqKey="Mounts A">AW Mounts</name></author><author><name sortKey="Group, Hnpsw" uniqKey="Group H">HNPSW group</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shrestha, Ss" uniqKey="Shrestha S">SS Shrestha</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lipatov, As" uniqKey="Lipatov A">AS Lipatov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matrosovich, M" uniqKey="Matrosovich M">M Matrosovich</name></author><author><name sortKey="Zhou, N" uniqKey="Zhou N">N Zhou</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author><author><name sortKey="Webster, R" uniqKey="Webster R">R Webster</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harfoot, R" uniqKey="Harfoot R">R Harfoot</name></author><author><name sortKey="Webby, Rj" uniqKey="Webby R">RJ Webby</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Herfst, S" uniqKey="Herfst S">S Herfst</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imai, M" uniqKey="Imai M">M Imai</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Osterholm, Mt" uniqKey="Osterholm M">MT Osterholm</name></author><author><name sortKey="Kelley, Ns" uniqKey="Kelley N">NS Kelley</name></author><author><name sortKey="Sommer, A" uniqKey="Sommer A">A Sommer</name></author><author><name sortKey="Belongia, Ea" uniqKey="Belongia E">EA Belongia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diazgranados, Ca" uniqKey="Diazgranados C">CA DiazGranados</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Fiers, W" uniqKey="Fiers W">W Fiers</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Nachbagauer, R" uniqKey="Nachbagauer R">R Nachbagauer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valkenburg, Sa" uniqKey="Valkenburg S">SA Valkenburg</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Wt" uniqKey="Yang W">WT Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, F" uniqKey="Gao F">F Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weaver, Ea" uniqKey="Weaver E">EA Weaver</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weaver, Ea" uniqKey="Weaver E">EA Weaver</name></author><author><name sortKey="Camacho, Zt" uniqKey="Camacho Z">ZT Camacho</name></author><author><name sortKey="Gao, F" uniqKey="Gao F">F Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ducatez, Mf" uniqKey="Ducatez M">MF Ducatez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laddy, Dj" uniqKey="Laddy D">DJ Laddy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weaver, Ea" uniqKey="Weaver E">EA Weaver</name></author><author><name sortKey="Rubrum, Am" uniqKey="Rubrum A">AM Rubrum</name></author><author><name sortKey="Webby, Rj" uniqKey="Webby R">RJ Webby</name></author><author><name sortKey="Barry, Ma" uniqKey="Barry M">MA Barry</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Benoit, A" uniqKey="Benoit A">A Benoit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagy, A" uniqKey="Nagy A">A Nagy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yan, J" uniqKey="Yan J">J Yan</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ouedraogo, A" uniqKey="Ouedraogo A">A Ouedraogo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baden, Lr" uniqKey="Baden L">LR Baden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="O Ara, Ga" uniqKey="O Ara G">GA O’Hara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="He, Xs" uniqKey="He X">XS He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Arevalo, Mt" uniqKey="Arevalo M">MT Arevalo</name></author><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name></author><author><name sortKey="Chen, S" uniqKey="Chen S">S Chen</name></author><author><name sortKey="Zeng, M" uniqKey="Zeng M">M Zeng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weaver, Ea" uniqKey="Weaver E">EA Weaver</name></author><author><name sortKey="Barry, Ma" uniqKey="Barry M">MA Barry</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Molinier Frenkel, V" uniqKey="Molinier Frenkel V">V Molinier-Frenkel</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ilyushina, Na" uniqKey="Ilyushina N">NA Ilyushina</name></author></analytic></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bullard, Brianna L" sort="Bullard, Brianna L" uniqKey="Bullard B" first="Brianna L." last="Bullard">Brianna L. Bullard</name><name sortKey="Lingel, Amy" sort="Lingel, Amy" uniqKey="Lingel A" first="Amy" last="Lingel">Amy Lingel</name><name sortKey="Weaver, Eric A" sort="Weaver, Eric A" uniqKey="Weaver E" first="Eric A." last="Weaver">Eric A. Weaver</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000324 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000324 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:5668234
|texte= Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29097763" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |