High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries
Identifieur interne : 000304 ( Main/Exploration ); précédent : 000303; suivant : 000305High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries
Auteurs : Ing-Chien Chen [Taïwan] ; Yi-Kai Chiu [Taïwan] ; Chung-Ming Yu [Taïwan] ; Cheng-Chung Lee [Taïwan] ; Chao-Ping Tung [Taïwan] ; Yueh-Liang Tsou [Taïwan] ; Yi-Jen Huang [Taïwan] ; Chia-Lung Lin [Taïwan] ; Hong-Sen Chen [Taïwan] ; Andrew H.-J. Wang [Taïwan] ; An-Suei Yang [Taïwan]Source :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
Pandemic and epidemic outbreaks of influenza A virus (IAV) infection pose severe challenges to human society. Passive immunotherapy with recombinant neutralizing antibodies can potentially mitigate the threats of IAV infection. With a high throughput neutralizing antibody discovery platform, we produced artificial anti-hemagglutinin (HA) IAV-neutralizing IgGs from phage-displayed synthetic scFv libraries without necessitating prior memory of antibody-antigen interactions or relying on affinity maturation essential for
Url:
DOI: 10.1038/s41598-017-14823-w
PubMed: 29089574
PubMed Central: 5663709
Affiliations:
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Taipei</wicri:regionArea></affiliation></author><author><name sortKey="Lee, Cheng Chung" sort="Lee, Cheng Chung" uniqKey="Lee C" first="Cheng-Chung" last="Lee">Cheng-Chung Lee</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2287 1366</institution-id><institution-id institution-id-type="GRID">grid.28665.3f</institution-id><institution>Institute of Biological Chemistry, Academia Sinica,</institution></institution-wrap>115 Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>115 Taipei</wicri:regionArea></affiliation></author><author><name sortKey="Tung, Chao Ping" sort="Tung, Chao Ping" uniqKey="Tung C" first="Chao-Ping" last="Tung">Chao-Ping Tung</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2287 1366</institution-id><institution-id 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wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2287 1366</institution-id><institution-id institution-id-type="GRID">grid.28665.3f</institution-id><institution>Genomics Research Center, Academia Sinica,</institution></institution-wrap>115 Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>115 Taipei</wicri:regionArea></affiliation></author><author><name sortKey="Lin, Chia Lung" sort="Lin, Chia Lung" uniqKey="Lin C" first="Chia-Lung" last="Lin">Chia-Lung Lin</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2287 1366</institution-id><institution-id institution-id-type="GRID">grid.28665.3f</institution-id><institution>Genomics Research Center, Academia Sinica,</institution></institution-wrap>115 Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>115 Taipei</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Hong Sen" sort="Chen, Hong Sen" uniqKey="Chen H" first="Hong-Sen" last="Chen">Hong-Sen Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2287 1366</institution-id><institution-id institution-id-type="GRID">grid.28665.3f</institution-id><institution>Genomics Research Center, Academia Sinica,</institution></institution-wrap>115 Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>115 Taipei</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Andrew H J" sort="Wang, Andrew H J" uniqKey="Wang A" first="Andrew H.-J." last="Wang">Andrew H.-J. Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2287 1366</institution-id><institution-id institution-id-type="GRID">grid.28665.3f</institution-id><institution>Institute of Biological Chemistry, Academia Sinica,</institution></institution-wrap>115 Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>115 Taipei</wicri:regionArea></affiliation></author><author><name sortKey="Yang, An Suei" sort="Yang, An Suei" uniqKey="Yang A" first="An-Suei" last="Yang">An-Suei Yang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2287 1366</institution-id><institution-id institution-id-type="GRID">grid.28665.3f</institution-id><institution>Genomics Research Center, Academia Sinica,</institution></institution-wrap>115 Taipei, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>115 Taipei</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Pandemic and epidemic outbreaks of influenza A virus (IAV) infection pose severe challenges to human society. Passive immunotherapy with recombinant neutralizing antibodies can potentially mitigate the threats of IAV infection. With a high throughput neutralizing antibody discovery platform, we produced artificial anti-hemagglutinin (HA) IAV-neutralizing IgGs from phage-displayed synthetic scFv libraries without necessitating prior memory of antibody-antigen interactions or relying on affinity maturation essential for <italic>in vivo</italic> immune systems to generate highly specific neutralizing antibodies. At least two thirds of the epitope groups of the artificial anti-HA antibodies resemble those of natural protective anti-HA antibodies, providing alternatives to neutralizing antibodies from natural antibody repertoires. With continuing advancement in designing and constructing synthetic scFv libraries, this technological platform is useful in mitigating not only the threats of IAV pandemics but also those from other newly emerging viral infections.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Wiersma, Lc" uniqKey="Wiersma L">LC Wiersma</name></author><author><name sortKey="Rimmelzwaan, Gf" uniqKey="Rimmelzwaan G">GF Rimmelzwaan</name></author><author><name sortKey="De Vries, Rd" uniqKey="De Vries R">RD de Vries</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Molinari, Na" uniqKey="Molinari N">NA Molinari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spanakis, N" uniqKey="Spanakis N">N Spanakis</name></author><author><name sortKey="Pitiriga, V" uniqKey="Pitiriga V">V Pitiriga</name></author><author><name sortKey="Gennimata, V" uniqKey="Gennimata V">V 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first="Hong-Sen" last="Chen">Hong-Sen Chen</name><name sortKey="Chiu, Yi Kai" sort="Chiu, Yi Kai" uniqKey="Chiu Y" first="Yi-Kai" last="Chiu">Yi-Kai Chiu</name><name sortKey="Huang, Yi Jen" sort="Huang, Yi Jen" uniqKey="Huang Y" first="Yi-Jen" last="Huang">Yi-Jen Huang</name><name sortKey="Lee, Cheng Chung" sort="Lee, Cheng Chung" uniqKey="Lee C" first="Cheng-Chung" last="Lee">Cheng-Chung Lee</name><name sortKey="Lin, Chia Lung" sort="Lin, Chia Lung" uniqKey="Lin C" first="Chia-Lung" last="Lin">Chia-Lung Lin</name><name sortKey="Tsou, Yueh Liang" sort="Tsou, Yueh Liang" uniqKey="Tsou Y" first="Yueh-Liang" last="Tsou">Yueh-Liang Tsou</name><name sortKey="Tung, Chao Ping" sort="Tung, Chao Ping" uniqKey="Tung C" first="Chao-Ping" last="Tung">Chao-Ping Tung</name><name sortKey="Wang, Andrew H J" sort="Wang, Andrew H J" uniqKey="Wang A" first="Andrew H.-J." last="Wang">Andrew H.-J. Wang</name><name sortKey="Yang, An Suei" sort="Yang, An Suei" uniqKey="Yang A" first="An-Suei" last="Yang">An-Suei Yang</name><name sortKey="Yu, Chung Ming" sort="Yu, Chung Ming" uniqKey="Yu C" first="Chung-Ming" last="Yu">Chung-Ming Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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