The T160A hemagglutinin substitution affects not only receptor binding property but also transmissibility of H5N1 clade 2.3.4 avian influenza virus in guinea pigs
Identifieur interne : 000263 ( Main/Exploration ); précédent : 000262; suivant : 000264The T160A hemagglutinin substitution affects not only receptor binding property but also transmissibility of H5N1 clade 2.3.4 avian influenza virus in guinea pigs
Auteurs : Min Gu ; Qunhui Li ; Ruyi Gao ; Dongchang He ; Yunpeng Xu ; Haixu Xu ; Lijun Xu ; Xiaoquan Wang ; Jiao Hu ; Xiaowen Liu ; Shunlin Hu ; Daxin Peng ; Xinan Jiao ; Xiufan LiuSource :
- Veterinary Research [ 0928-4249 ] ; 2017.
Abstract
We generated and characterized site-directed HA mutants on the genetic backbone of H5N1 clade 2.3.4 virus preferentially binding to α-2,3 receptors in order to identify the key determinants in hemagglutinin rendering the dual affinity to both α-2,3 (avian-type) and α-2,6 (human-type) linked sialic acid receptors of the current clade 2.3.4.4 H5NX subtype avian influenza reassortants. The results show that the T160A substitution resulted in the loss of a glycosylation site at 158N and led not only to enhanced binding specificity for human-type receptors but also transmissibility among guinea pigs, which could be considered as an important molecular marker for assessing pandemic potential of H5 subtype avian influenza isolates.
The online version of this article (doi:10.1186/s13567-017-0410-0) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s13567-017-0410-0
PubMed: 28166830
PubMed Central: 5294818
Affiliations:
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University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author><author><name sortKey="Li, Qunhui" sort="Li, Qunhui" uniqKey="Li Q" first="Qunhui" last="Li">Qunhui Li</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Veterinary Medicine,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author><author><name sortKey="Gao, Ruyi" sort="Gao, Ruyi" uniqKey="Gao R" first="Ruyi" last="Gao">Ruyi Gao</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Veterinary Medicine,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author><author><name sortKey="He, Dongchang" sort="He, Dongchang" uniqKey="He D" first="Dongchang" last="He">Dongchang He</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Veterinary Medicine,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author><author><name sortKey="Xu, Yunpeng" sort="Xu, Yunpeng" uniqKey="Xu Y" first="Yunpeng" last="Xu">Yunpeng Xu</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Veterinary Medicine,</institution><institution>Yangzhou 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225009 China</wicri:noCountry></affiliation></author><author><name sortKey="Hu, Shunlin" sort="Hu, Shunlin" uniqKey="Hu S" first="Shunlin" last="Hu">Shunlin Hu</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Veterinary Medicine,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>Jiangsu Key Laboratory of Zoonosis,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author><author><name sortKey="Peng, Daxin" sort="Peng, Daxin" uniqKey="Peng D" 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code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author><author><name sortKey="Jiao, Xinan" sort="Jiao, Xinan" uniqKey="Jiao X" first="Xinan" last="Jiao">Xinan Jiao</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Veterinary Medicine,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>Jiangsu Key Laboratory of Zoonosis,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff4">Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author><author><name sortKey="Liu, Xiufan" sort="Liu, Xiufan" uniqKey="Liu X" first="Xiufan" last="Liu">Xiufan Liu</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>College of Veterinary Medicine,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.268415.c</institution-id><institution>Jiangsu Key Laboratory of Zoonosis,</institution><institution>Yangzhou University,</institution></institution-wrap>Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff4">Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu 225009 China</nlm:aff><wicri:noCountry code="subfield">Jiangsu 225009 China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Veterinary Research</title><idno type="ISSN">0928-4249</idno><idno type="eISSN">1297-9716</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">We generated and characterized site-directed HA mutants on the genetic backbone of H5N1 clade 2.3.4 virus preferentially binding to α-2,3 receptors in order to identify the key determinants in hemagglutinin rendering the dual affinity to both α-2,3 (avian-type) and α-2,6 (human-type) linked sialic acid receptors of the current clade 2.3.4.4 H5NX subtype avian influenza reassortants. The results show that the T160A substitution resulted in the loss of a glycosylation site at 158N and led not only to enhanced binding specificity for human-type receptors but also transmissibility among guinea pigs, which could be considered as an important molecular marker for assessing pandemic potential of H5 subtype avian influenza isolates.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s13567-017-0410-0) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Smith, Gj" uniqKey="Smith G">GJ Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watanabe, Y" uniqKey="Watanabe Y">Y Watanabe</name></author><author><name sortKey="Ibrahim, Ms" uniqKey="Ibrahim M">MS 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Shinya</name></author><author><name sortKey="Deng, G" uniqKey="Deng G">G Deng</name></author><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y Jiang</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z Li</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Tian, G" uniqKey="Tian G">G Tian</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Shi, J" uniqKey="Shi J">J Shi</name></author><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Zeng, X" uniqKey="Zeng X">X Zeng</name></author><author><name sortKey="Bu, Z" uniqKey="Bu Z">Z Bu</name></author><author><name sortKey="Xia, X" uniqKey="Xia X">X Xia</name></author><author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y Kawaoka</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Gao, Ruyi" sort="Gao, Ruyi" uniqKey="Gao R" first="Ruyi" last="Gao">Ruyi Gao</name><name sortKey="Gu, Min" sort="Gu, Min" uniqKey="Gu M" first="Min" last="Gu">Min Gu</name><name sortKey="He, Dongchang" sort="He, Dongchang" uniqKey="He D" first="Dongchang" last="He">Dongchang He</name><name sortKey="Hu, Jiao" sort="Hu, Jiao" uniqKey="Hu J" first="Jiao" last="Hu">Jiao Hu</name><name sortKey="Hu, Shunlin" sort="Hu, Shunlin" uniqKey="Hu S" first="Shunlin" last="Hu">Shunlin Hu</name><name sortKey="Jiao, Xinan" sort="Jiao, Xinan" uniqKey="Jiao X" first="Xinan" last="Jiao">Xinan Jiao</name><name sortKey="Li, Qunhui" sort="Li, Qunhui" uniqKey="Li Q" first="Qunhui" last="Li">Qunhui Li</name><name sortKey="Liu, Xiaowen" sort="Liu, Xiaowen" uniqKey="Liu X" first="Xiaowen" last="Liu">Xiaowen Liu</name><name sortKey="Liu, Xiufan" sort="Liu, Xiufan" uniqKey="Liu X" first="Xiufan" last="Liu">Xiufan Liu</name><name sortKey="Peng, Daxin" sort="Peng, Daxin" uniqKey="Peng D" first="Daxin" last="Peng">Daxin Peng</name><name sortKey="Wang, Xiaoquan" sort="Wang, Xiaoquan" uniqKey="Wang X" first="Xiaoquan" last="Wang">Xiaoquan Wang</name><name sortKey="Xu, Haixu" sort="Xu, Haixu" uniqKey="Xu H" first="Haixu" last="Xu">Haixu Xu</name><name sortKey="Xu, Lijun" sort="Xu, Lijun" uniqKey="Xu L" first="Lijun" last="Xu">Lijun Xu</name><name sortKey="Xu, Yunpeng" sort="Xu, Yunpeng" uniqKey="Xu Y" first="Yunpeng" last="Xu">Yunpeng Xu</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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