Safety, immunogenicity and protection of A(H3N2) live attenuated influenza vaccines containing wild-type nucleoprotein in a ferret model.
Identifieur interne : 000160 ( Main/Exploration ); précédent : 000159; suivant : 000161Safety, immunogenicity and protection of A(H3N2) live attenuated influenza vaccines containing wild-type nucleoprotein in a ferret model.
Auteurs : Daniil A. Korenkov [Russie] ; Karen L. Laurie [Australie] ; Patrick C. Reading [Australie] ; Louise A. Carolan [Australie] ; Kok Fei Chan [Australie] ; Irina I. Isakova-Sivak [Russie] ; Tatiana A. Smolonogina [Russie] ; Kanta Subbarao [Australie] ; Ian G. Barr [Australie] ; Julie Villanueva [États-Unis] ; Svetlana Shcherbik [États-Unis] ; Tatiana Bousse [États-Unis] ; Larisa G. Rudenko [Russie]Source :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Anticorps neutralisants (immunologie), Anticorps neutralisants (sang), Femelle, Furets, Génome viral, Immunogénicité des vaccins, Infections à Orthomyxoviridae (médecine vétérinaire), Maladies de l'animal (), Maladies de l'animal (immunologie), Maladies de l'animal (virologie), Modèles animaux de maladie humaine, Mâle, Nucléoprotéines (génétique), Nucléoprotéines (immunologie), Sous-type H3N2 du virus de la grippe A (génétique), Sous-type H3N2 du virus de la grippe A (immunologie), Tests de neutralisation, Vaccination, Vaccins antigrippaux (effets indésirables), Vaccins antigrippaux (génétique), Vaccins antigrippaux (immunologie), Vaccins atténués (effets indésirables), Vaccins atténués (génétique), Vaccins atténués (immunologie).
- MESH :
- effets indésirables : Vaccins antigrippaux, Vaccins atténués.
- génétique : Nucléoprotéines, Sous-type H3N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués.
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Maladies de l'animal, Nucléoprotéines, Sous-type H3N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués.
- médecine vétérinaire : Infections à Orthomyxoviridae.
- sang : Anticorps antiviraux, Anticorps neutralisants.
- virologie : Maladies de l'animal.
- Animaux, Femelle, Furets, Génome viral, Immunogénicité des vaccins, Maladies de l'animal, Modèles animaux de maladie humaine, Mâle, Tests de neutralisation, Vaccination.
English descriptors
- KwdEn :
- Animal Diseases (immunology), Animal Diseases (prevention & control), Animal Diseases (virology), Animals, Antibodies, Neutralizing (blood), Antibodies, Neutralizing (immunology), Antibodies, Viral (blood), Antibodies, Viral (immunology), Disease Models, Animal, Female, Ferrets, Genome, Viral, Immunogenicity, Vaccine, Influenza A Virus, H3N2 Subtype (genetics), Influenza A Virus, H3N2 Subtype (immunology), Influenza Vaccines (adverse effects), Influenza Vaccines (genetics), Influenza Vaccines (immunology), Male, Neutralization Tests, Nucleoproteins (genetics), Nucleoproteins (immunology), Orthomyxoviridae Infections (veterinary), Vaccination, Vaccines, Attenuated (adverse effects), Vaccines, Attenuated (genetics), Vaccines, Attenuated (immunology).
- MESH :
- chemical , adverse effects : Influenza Vaccines, Vaccines, Attenuated.
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- genetics : Influenza A Virus, H3N2 Subtype, Influenza Vaccines, Nucleoproteins, Vaccines, Attenuated.
- immunology : Animal Diseases, Antibodies, Neutralizing, Antibodies, Viral, Influenza A Virus, H3N2 Subtype, Influenza Vaccines, Nucleoproteins, Vaccines, Attenuated.
- prevention & control : Animal Diseases.
- veterinary : Orthomyxoviridae Infections.
- virology : Animal Diseases.
- Animals, Disease Models, Animal, Female, Ferrets, Genome, Viral, Immunogenicity, Vaccine, Male, Neutralization Tests, Vaccination.
Abstract
Live attenuated influenza vaccines (LAIVs) are promising tools for the induction of broad protection from influenza due to their ability to stimulate cross-reactive T cells against influenza pathogens. One of the major targets for cytotoxic T-cell immunity is viral nucleoprotein (NP), which is relatively conserved among antigenically distant influenza viruses. Nevertheless, a diversity of epitope composition has been found in the NP protein of different lineages of influenza A viruses. The H2N2 master donor virus which is currently used as a backbone for the LAIV and donor of the six genomic segments encoding the internal proteins, A/Leningrad/134/17/57 (MDV Len/17), was isolated 60 years ago. As such, NP-specific T-cell immunity induced upon vaccination with classical LAIVs with a 6:2 genome composition containing this older NP might be suboptimal against currently circulating influenza viruses. In this study, a panel of H3N2 LAIV candidates with wild-type NP genes derived from circulating viruses were generated by reverse genetics (5:3 genome composition). These viruses displayed the cold adaptation and temperature sensitivity phenotypes of MDV Len/17 in vitro. LAIVs with both 6:2 and 5:3 genome compositions were attenuated and replicated to a similar extent in the upper respiratory tract of ferrets. LAIVs were immunogenic as high neutralizing and hemagglutination inhibition serum antibody titers were detected 21 days after infection. All vaccinated animals were protected against infection with heterologous H3N2 influenza A viruses. Thus, LAIV with a 5:3 genome composition is safe, immunogenic and can induce cross-protective immunity.
DOI: 10.1016/j.meegid.2018.06.019
PubMed: 29929009
Affiliations:
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Le document en format XML
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Korenkov</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Experimental Medicine, 12, acad. Pavlova street, Saint Petersburg, Russia. Electronic address: d.korenkov@gmail.com.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Virology, Institute of Experimental Medicine, 12, acad. Pavlova street, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Laurie, Karen L" sort="Laurie, Karen L" uniqKey="Laurie K" first="Karen L" last="Laurie">Karen L. Laurie</name><affiliation wicri:level="3"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Reading, Patrick C" sort="Reading, Patrick C" uniqKey="Reading P" first="Patrick C" last="Reading">Patrick C. Reading</name><affiliation wicri:level="3"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Carolan, Louise A" sort="Carolan, Louise A" uniqKey="Carolan L" first="Louise A" last="Carolan">Louise A. Carolan</name><affiliation wicri:level="3"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Chan, Kok Fei" sort="Chan, Kok Fei" uniqKey="Chan K" first="Kok Fei" last="Chan">Kok Fei Chan</name><affiliation wicri:level="3"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Isakova Sivak, Irina I" sort="Isakova Sivak, Irina I" uniqKey="Isakova Sivak I" first="Irina I" last="Isakova-Sivak">Irina I. Isakova-Sivak</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Experimental Medicine, 12, acad. Pavlova street, Saint Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Virology, Institute of Experimental Medicine, 12, acad. Pavlova street, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Smolonogina, Tatiana A" sort="Smolonogina, Tatiana A" uniqKey="Smolonogina T" first="Tatiana A" last="Smolonogina">Tatiana A. Smolonogina</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Experimental Medicine, 12, acad. Pavlova street, Saint Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Virology, Institute of Experimental Medicine, 12, acad. Pavlova street, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name><affiliation wicri:level="3"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G" last="Barr">Ian G. Barr</name><affiliation wicri:level="3"><nlm:affiliation>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory at The Peter Doherty Institute for Infection & Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Villanueva, Julie" sort="Villanueva, Julie" uniqKey="Villanueva J" first="Julie" last="Villanueva">Julie Villanueva</name><affiliation wicri:level="1"><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, GA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, GA</wicri:regionArea><wicri:noRegion>GA</wicri:noRegion></affiliation></author><author><name sortKey="Shcherbik, Svetlana" sort="Shcherbik, Svetlana" uniqKey="Shcherbik S" first="Svetlana" last="Shcherbik">Svetlana Shcherbik</name><affiliation wicri:level="1"><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, GA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, GA</wicri:regionArea><wicri:noRegion>GA</wicri:noRegion></affiliation></author><author><name sortKey="Bousse, Tatiana" sort="Bousse, Tatiana" uniqKey="Bousse T" first="Tatiana" last="Bousse">Tatiana Bousse</name><affiliation wicri:level="1"><nlm:affiliation>Centers for Disease Control and Prevention, Atlanta, GA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Centers for Disease Control and Prevention, Atlanta, GA</wicri:regionArea><wicri:noRegion>GA</wicri:noRegion></affiliation></author><author><name sortKey="Rudenko, Larisa G" sort="Rudenko, Larisa G" uniqKey="Rudenko L" first="Larisa G" last="Rudenko">Larisa G. Rudenko</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Institute of Experimental Medicine, 12, acad. Pavlova street, Saint Petersburg, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Virology, Institute of Experimental Medicine, 12, acad. 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(genetics)</term><term>Influenza A Virus, H3N2 Subtype (immunology)</term><term>Influenza Vaccines (adverse effects)</term><term>Influenza Vaccines (genetics)</term><term>Influenza Vaccines (immunology)</term><term>Male</term><term>Neutralization Tests</term><term>Nucleoproteins (genetics)</term><term>Nucleoproteins (immunology)</term><term>Orthomyxoviridae Infections (veterinary)</term><term>Vaccination</term><term>Vaccines, Attenuated (adverse effects)</term><term>Vaccines, Attenuated (genetics)</term><term>Vaccines, Attenuated (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps antiviraux (sang)</term><term>Anticorps neutralisants (immunologie)</term><term>Anticorps neutralisants (sang)</term><term>Femelle</term><term>Furets</term><term>Génome viral</term><term>Immunogénicité des vaccins</term><term>Infections à Orthomyxoviridae (médecine vétérinaire)</term><term>Maladies de l'animal ()</term><term>Maladies de l'animal (immunologie)</term><term>Maladies de l'animal (virologie)</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Nucléoprotéines (génétique)</term><term>Nucléoprotéines (immunologie)</term><term>Sous-type H3N2 du virus de la grippe A (génétique)</term><term>Sous-type H3N2 du virus de la grippe A (immunologie)</term><term>Tests de neutralisation</term><term>Vaccination</term><term>Vaccins antigrippaux (effets indésirables)</term><term>Vaccins antigrippaux (génétique)</term><term>Vaccins antigrippaux (immunologie)</term><term>Vaccins atténués (effets indésirables)</term><term>Vaccins atténués (génétique)</term><term>Vaccins atténués (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Influenza Vaccines</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="effets indésirables" xml:lang="fr"><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza Vaccines</term><term>Nucleoproteins</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Nucléoprotéines</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term><term>Maladies de l'animal</term><term>Nucléoprotéines</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Animal Diseases</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza Vaccines</term><term>Nucleoproteins</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Animal Diseases</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps neutralisants</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies de l'animal</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Animal Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Female</term><term>Ferrets</term><term>Genome, Viral</term><term>Immunogenicity, Vaccine</term><term>Male</term><term>Neutralization Tests</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Furets</term><term>Génome viral</term><term>Immunogénicité des vaccins</term><term>Maladies de l'animal</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Tests de neutralisation</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Live attenuated influenza vaccines (LAIVs) are promising tools for the induction of broad protection from influenza due to their ability to stimulate cross-reactive T cells against influenza pathogens. One of the major targets for cytotoxic T-cell immunity is viral nucleoprotein (NP), which is relatively conserved among antigenically distant influenza viruses. Nevertheless, a diversity of epitope composition has been found in the NP protein of different lineages of influenza A viruses. The H2N2 master donor virus which is currently used as a backbone for the LAIV and donor of the six genomic segments encoding the internal proteins, A/Leningrad/134/17/57 (MDV Len/17), was isolated 60 years ago. As such, NP-specific T-cell immunity induced upon vaccination with classical LAIVs with a 6:2 genome composition containing this older NP might be suboptimal against currently circulating influenza viruses. In this study, a panel of H3N2 LAIV candidates with wild-type NP genes derived from circulating viruses were generated by reverse genetics (5:3 genome composition). These viruses displayed the cold adaptation and temperature sensitivity phenotypes of MDV Len/17 in vitro. LAIVs with both 6:2 and 5:3 genome compositions were attenuated and replicated to a similar extent in the upper respiratory tract of ferrets. LAIVs were immunogenic as high neutralizing and hemagglutination inhibition serum antibody titers were detected 21 days after infection. All vaccinated animals were protected against infection with heterologous H3N2 influenza A viruses. Thus, LAIV with a 5:3 genome composition is safe, immunogenic and can induce cross-protective immunity.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Russie</li><li>États-Unis</li></country><region><li>Victoria (État)</li></region><settlement><li>Melbourne</li></settlement></list><tree><country name="Russie"><noRegion><name sortKey="Korenkov, Daniil A" sort="Korenkov, Daniil A" uniqKey="Korenkov D" first="Daniil A" last="Korenkov">Daniil A. Korenkov</name></noRegion><name sortKey="Isakova Sivak, Irina I" sort="Isakova Sivak, Irina I" uniqKey="Isakova Sivak I" first="Irina I" last="Isakova-Sivak">Irina I. Isakova-Sivak</name><name sortKey="Rudenko, Larisa G" sort="Rudenko, Larisa G" uniqKey="Rudenko L" first="Larisa G" last="Rudenko">Larisa G. Rudenko</name><name sortKey="Smolonogina, Tatiana A" sort="Smolonogina, Tatiana A" uniqKey="Smolonogina T" first="Tatiana A" last="Smolonogina">Tatiana A. Smolonogina</name></country><country name="Australie"><region name="Victoria (État)"><name sortKey="Laurie, Karen L" sort="Laurie, Karen L" uniqKey="Laurie K" first="Karen L" last="Laurie">Karen L. Laurie</name></region><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G" last="Barr">Ian G. Barr</name><name sortKey="Carolan, Louise A" sort="Carolan, Louise A" uniqKey="Carolan L" first="Louise A" last="Carolan">Louise A. Carolan</name><name sortKey="Chan, Kok Fei" sort="Chan, Kok Fei" uniqKey="Chan K" first="Kok Fei" last="Chan">Kok Fei Chan</name><name sortKey="Reading, Patrick C" sort="Reading, Patrick C" uniqKey="Reading P" first="Patrick C" last="Reading">Patrick C. Reading</name><name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name></country><country name="États-Unis"><noRegion><name sortKey="Villanueva, Julie" sort="Villanueva, Julie" uniqKey="Villanueva J" first="Julie" last="Villanueva">Julie Villanueva</name></noRegion><name sortKey="Bousse, Tatiana" sort="Bousse, Tatiana" uniqKey="Bousse T" first="Tatiana" last="Bousse">Tatiana Bousse</name><name sortKey="Shcherbik, Svetlana" sort="Shcherbik, Svetlana" uniqKey="Shcherbik S" first="Svetlana" last="Shcherbik">Svetlana Shcherbik</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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