Correlation between RNA fragments of fowl plague virus and their corresponding gene functions
Identifieur interne : 002B29 ( Main/Curation ); précédent : 002B28; suivant : 002B30Correlation between RNA fragments of fowl plague virus and their corresponding gene functions
Auteurs : C. Scholtissek [Allemagne] ; Etti Harms [Allemagne] ; W. Rohde [Allemagne] ; Michaela Orlich [Allemagne] ; R. Rott [Allemagne]Source :
- Virology [ 0042-6822 ] ; 1976.
English descriptors
- Teeft :
- Academic press, Base sequence homology, Chick, Chick embryo cells, Compans, Crna, Digestion, Double infection, Embryo, Equal volume, First fraction, Formaldehyde, Fowl, Fowl plague virus, Fragment, Gene, Gene product, Hemagglutinin, Homology, Hybrid, Hybridization, Hybridization studies, Hybridized, Individual fragments, Influenza, Influenza strain, Influenza virus, Influenza viruses, Input radioactivity, Molecular weight, Mutant, Neuraminidase, Nonlabeled, Nonlabeled crna, Other strains, Petri dishes, Plaque, Plaque formation, Plaque morphology, Polyacrylamide, Primary chick embryo cells, Prototype strains, Recombinant, Recombination, Rnase, Rnase digestion, Rnase resistance, Rott, Scholtissek, Specific recombinants, Urea, Various influenza, Virology, Virus.
Abstract
Abstract: Fowl plague virus (FPV) RNA consists of eight fragments which, except for the two fragments with the highest molecular weight, can be clearly separated by polyacrylamide gel electrophoresis in the presence of 8 M urea. The base sequence homology between individual fragments of FPV RNA and the complementary RNA (cRNA) of various influenza A prototype strains has been established. Recombinants have been isolated by double infection of chick embryo cells with ts mutants of FPV and influenza A strains, which do not form plaques on this host. By hybridization of the cRNA of these recombinants with the 32P-labeled fragments of FPV RNA six of the eight RNA fragments can be correlated with gene functions of FPV.
Url:
DOI: 10.1016/0042-6822(76)90340-8
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<term>Chick embryo cells</term>
<term>Compans</term>
<term>Crna</term>
<term>Digestion</term>
<term>Double infection</term>
<term>Embryo</term>
<term>Equal volume</term>
<term>First fraction</term>
<term>Formaldehyde</term>
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<term>Fowl plague virus</term>
<term>Fragment</term>
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<term>Gene product</term>
<term>Hemagglutinin</term>
<term>Homology</term>
<term>Hybrid</term>
<term>Hybridization</term>
<term>Hybridization studies</term>
<term>Hybridized</term>
<term>Individual fragments</term>
<term>Influenza</term>
<term>Influenza strain</term>
<term>Influenza virus</term>
<term>Influenza viruses</term>
<term>Input radioactivity</term>
<term>Molecular weight</term>
<term>Mutant</term>
<term>Neuraminidase</term>
<term>Nonlabeled</term>
<term>Nonlabeled crna</term>
<term>Other strains</term>
<term>Petri dishes</term>
<term>Plaque</term>
<term>Plaque formation</term>
<term>Plaque morphology</term>
<term>Polyacrylamide</term>
<term>Primary chick embryo cells</term>
<term>Prototype strains</term>
<term>Recombinant</term>
<term>Recombination</term>
<term>Rnase</term>
<term>Rnase digestion</term>
<term>Rnase resistance</term>
<term>Rott</term>
<term>Scholtissek</term>
<term>Specific recombinants</term>
<term>Urea</term>
<term>Various influenza</term>
<term>Virology</term>
<term>Virus</term>
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<front><div type="abstract" xml:lang="en">Abstract: Fowl plague virus (FPV) RNA consists of eight fragments which, except for the two fragments with the highest molecular weight, can be clearly separated by polyacrylamide gel electrophoresis in the presence of 8 M urea. The base sequence homology between individual fragments of FPV RNA and the complementary RNA (cRNA) of various influenza A prototype strains has been established. Recombinants have been isolated by double infection of chick embryo cells with ts mutants of FPV and influenza A strains, which do not form plaques on this host. By hybridization of the cRNA of these recombinants with the 32P-labeled fragments of FPV RNA six of the eight RNA fragments can be correlated with gene functions of FPV.</div>
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