Growth of some attenuated influenza viruses in hamster tracheal organ cultures
Identifieur interne : 002A19 ( Main/Curation ); précédent : 002A18; suivant : 002A20Growth of some attenuated influenza viruses in hamster tracheal organ cultures
Auteurs : P. Reeve [Autriche] ; Batia Gerendas [Autriche] ; H. Walzl [Autriche]Source :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 1978-11-01.
English descriptors
- Teeft :
- Assay, Attenuated, Attenuated influenza viruses, Attenuation, Chanock, Ciliary, Ciliary activity, Dos, Epithelium, Hamster, Hamster tracheal cultures, Hamster tracheal organ cultures, High doses, Histological, Human volunteers, Influenza, Influenza vaccines, Influenza virus, Influenza viruses, Maassab, Metabolic, Metabolic activity, Mutant, Organ cultures, Reeve, Relative virulence, Tetrazolium, Tetrazolium reduction assay, Tracheal, Tracheal organ cultures, Tracheal rings, Vaccine, Virus, Virus inoculated.
Abstract
Abstract: Hamster tracheal organ cultures were infected with different influenza viruses and the metabolic activity measured using a tetrazolium reduction assay. In addition, relative ciliary activity was observed, virus multiplication measured, and histological studies were made. The hamster organ cultures proved relatively simple to set up and were sensitive to infection with influenza viruses; and the tetrazolium reduction was a reliable objective measure of relative tissue damage correlating well with histological and virological findings as well as with visual assessment of ciliary activities.
Url:
DOI: 10.1007/BF02121143
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<term>Ciliary</term>
<term>Ciliary activity</term>
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<term>Hamster tracheal cultures</term>
<term>Hamster tracheal organ cultures</term>
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<front><div type="abstract" xml:lang="en">Abstract: Hamster tracheal organ cultures were infected with different influenza viruses and the metabolic activity measured using a tetrazolium reduction assay. In addition, relative ciliary activity was observed, virus multiplication measured, and histological studies were made. The hamster organ cultures proved relatively simple to set up and were sensitive to infection with influenza viruses; and the tetrazolium reduction was a reliable objective measure of relative tissue damage correlating well with histological and virological findings as well as with visual assessment of ciliary activities.</div>
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