Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies
Identifieur interne : 002655 ( Main/Curation ); précédent : 002654; suivant : 002656Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies
Auteurs : R. G. Webster [États-Unis] ; V. S. Hinshaw [États-Unis] ; W. G. Laver [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1982.
English descriptors
- Teeft :
- Amino, Amino acid sequence, Antibody, Antigenic, Antigenic drift, Antigenic variants, Antigenic variation, Assay, Avian, Avian strains, Blok, Chick embryos, Elisa, Enzyme activity, Hemagglutinin, Hemagglutinin activity, High concentrations, Hinshaw, Influenza, Influenza type, Influenza virus, Influenza virus neuraminidase, Influenza viruses, Kilbourne, Laver, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoclonal antibody preparations, Neuraminidase, Neuraminidase activity, Neuraminidase gene, Neuraminidase inhibition tests, Neuraminidase molecule, Neuraminidase molecules, Neuraminidases, Plaque size, Reactivity patterns, Single passage, Variant, Virus.
Abstract
Abstract: Antigenic variants of the neuraminidase (NA) of A/Tokyo/3/67 influenza virus (H2N2) were selected with a frequency of approximately 1 in 105 after a single passage of the virus in the presence of monoclonal antibody to the NA. Analysis of a panel of antigenic variants with N2 monoclonal antibodies provided evidence for at least three nonover-lapping antigenic areas on the neuraminidase molecule of A/Tokyo/3/67 and possibly four. Analysis of field strains showed that antigenic variation in the neuraminidase was evident even between influenza virus isolated in the first year following the emergence of a new pandemic strain. A/Japan/305/57 differed in at least seven epitopes from A/ Okuda/57 and A/RI/5+/57. Antigenic drift occurred in each of the antigenic areas on the neuraminidase of human influenza virus from 1957 to 1979 with a gradual loss in reactivity with monoclonal antibodies to the early strains and acquisition of reactivity with antibodies to later strains. Only limited antigenic variation was detectable in the N2 neuraminidase for avian strains, over a similar time period; all of the avian N2 neuraminidases were closely related to the neuraminidase on A/Japan/305/57. Hemagglutination inhibition by monoclonal antibodies to the NA occurred in some but not all N2 viruses. These results suggest that the distribution of neuraminidase molecules may differ among viruses possessing N2 neuraminidase.
Url:
DOI: 10.1016/0042-6822(82)90510-4
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000066
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :000066
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001297
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :002793
Links to Exploration step
ISTEX:1400662980D890C0235684657B9D7F1046E59063Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies</title>
<author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R. G." last="Webster">R. G. Webster</name>
</author>
<author><name sortKey="Hinshaw, V S" sort="Hinshaw, V S" uniqKey="Hinshaw V" first="V. S." last="Hinshaw">V. S. Hinshaw</name>
</author>
<author><name sortKey="Laver, W G" sort="Laver, W G" uniqKey="Laver W" first="W. G." last="Laver">W. G. Laver</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:1400662980D890C0235684657B9D7F1046E59063</idno>
<date when="1982" year="1982">1982</date>
<idno type="doi">10.1016/0042-6822(82)90510-4</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-FBCLDKN6-T/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000066</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000066</idno>
<idno type="wicri:Area/Istex/Curation">000066</idno>
<idno type="wicri:Area/Istex/Checkpoint">001297</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001297</idno>
<idno type="wicri:doubleKey">0042-6822:1982:Webster R:selection:and:analysis</idno>
<idno type="wicri:Area/Main/Merge">002793</idno>
<idno type="wicri:Area/Main/Curation">002655</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies</title>
<author><name sortKey="Webster, R G" sort="Webster, R G" uniqKey="Webster R" first="R. G." last="Webster">R. G. Webster</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, Tennessee 38101</wicri:regionArea>
<wicri:noRegion>Tennessee 38101</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hinshaw, V S" sort="Hinshaw, V S" uniqKey="Hinshaw V" first="V. S." last="Hinshaw">V. S. Hinshaw</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, Tennessee 38101</wicri:regionArea>
<wicri:noRegion>Tennessee 38101</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Laver, W G" sort="Laver, W G" uniqKey="Laver W" first="W. G." last="Laver">W. G. Laver</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>St. Jude Children's Research Hospital, 332 North Lauderdale, Memphis, Tennessee 38101</wicri:regionArea>
<wicri:noRegion>Tennessee 38101</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="ISSN">0042-6822</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1982">1982</date>
<biblScope unit="volume">117</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="93">93</biblScope>
<biblScope unit="page" to="104">104</biblScope>
</imprint>
<idno type="ISSN">0042-6822</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0042-6822</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term>
<term>Amino acid sequence</term>
<term>Antibody</term>
<term>Antigenic</term>
<term>Antigenic drift</term>
<term>Antigenic variants</term>
<term>Antigenic variation</term>
<term>Assay</term>
<term>Avian</term>
<term>Avian strains</term>
<term>Blok</term>
<term>Chick embryos</term>
<term>Elisa</term>
<term>Enzyme activity</term>
<term>Hemagglutinin</term>
<term>Hemagglutinin activity</term>
<term>High concentrations</term>
<term>Hinshaw</term>
<term>Influenza</term>
<term>Influenza type</term>
<term>Influenza virus</term>
<term>Influenza virus neuraminidase</term>
<term>Influenza viruses</term>
<term>Kilbourne</term>
<term>Laver</term>
<term>Monoclonal</term>
<term>Monoclonal antibodies</term>
<term>Monoclonal antibody</term>
<term>Monoclonal antibody preparations</term>
<term>Neuraminidase</term>
<term>Neuraminidase activity</term>
<term>Neuraminidase gene</term>
<term>Neuraminidase inhibition tests</term>
<term>Neuraminidase molecule</term>
<term>Neuraminidase molecules</term>
<term>Neuraminidases</term>
<term>Plaque size</term>
<term>Reactivity patterns</term>
<term>Single passage</term>
<term>Variant</term>
<term>Virus</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Antigenic variants of the neuraminidase (NA) of A/Tokyo/3/67 influenza virus (H2N2) were selected with a frequency of approximately 1 in 105 after a single passage of the virus in the presence of monoclonal antibody to the NA. Analysis of a panel of antigenic variants with N2 monoclonal antibodies provided evidence for at least three nonover-lapping antigenic areas on the neuraminidase molecule of A/Tokyo/3/67 and possibly four. Analysis of field strains showed that antigenic variation in the neuraminidase was evident even between influenza virus isolated in the first year following the emergence of a new pandemic strain. A/Japan/305/57 differed in at least seven epitopes from A/ Okuda/57 and A/RI/5+/57. Antigenic drift occurred in each of the antigenic areas on the neuraminidase of human influenza virus from 1957 to 1979 with a gradual loss in reactivity with monoclonal antibodies to the early strains and acquisition of reactivity with antibodies to later strains. Only limited antigenic variation was detectable in the N2 neuraminidase for avian strains, over a similar time period; all of the avian N2 neuraminidases were closely related to the neuraminidase on A/Japan/305/57. Hemagglutination inhibition by monoclonal antibodies to the NA occurred in some but not all N2 viruses. These results suggest that the distribution of neuraminidase molecules may differ among viruses possessing N2 neuraminidase.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002655 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002655 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= H2N2V1 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:1400662980D890C0235684657B9D7F1046E59063 |texte= Selection and analysis of antigenic variants of the neuraminidase of N2 influenza viruses with monoclonal antibodies }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |