Intracellular Development of Membrane Protein of Influenza Virus
Identifieur interne : 000352 ( Istex/Curation ); précédent : 000351; suivant : 000353Intracellular Development of Membrane Protein of Influenza Virus
Auteurs : Koichiro Maeno [Japon] ; Saiji Yoshii ; Tetsuya Yoshida ; Masao Iinuma ; Yasuko Kawamoto ; Toshisada MatsumotoSource :
- Microbiology and Immunology [ 0385-5600 ] ; 1977-08.
English descriptors
- Teeft :
- Abortive, Abortive infection, Academic press, Antigen, Antiserum, Biological activities, Cell monolayers, Cell surface, Clone, Clone cells, Continuous lines, Culture fluids, Cytoplasmic fluorescence, Developmental sequence, Diffuse fluorescence, Fowl plague virus, Further incubation, Immunofluorescent staining, Infection, Influenza, Influenza virus, Influenza viruses, Input multiplicity, Intracellular, Intracellular development, Intracellular sites, Maeno, Maintenance medium, Membrane protein, Monospecific antiserum, Newcastle disease virus, Nonreducing conditions, Nonstructural proteins, Nuclear fluorescence, Nuclear regions, Paper strips, Peripheral wells, Phosphate buffer, Plasma membrane, Precipitin lines, Productive infection, Protein antigens, Recent studies, Room temperature, Sendai virus, Single precipitin line, Stronger fluorescence, Various times, Veronal buffer, Viral, Virion, Virology, Virus, Whole cell.
Abstract
The intracellular development of membrane protein (MP) of influenza A virus was investigated by immunofluorescent staining. Monospecific antiserum was prepared by immunizing rabbits with MP eluted from SDS‐polyacrylamide gels of SDS‐disrupted NWS virions. In the productive infection in clone 1‐5C‐4 cells, MP antigen was first detected over the whole cell at 4 hr after infection, concomitantly with the appearance of hemagglutinin (HA) antigen in the cytoplasm, and bright nuclear fluorescence was then observed. Nucleoprotein (NP) antigen was detected in the nucleus prior to the appearance of fluorescence of MP antigen and thereafter the cytoplasmic fluorescence developed. Late in infection, all of these three antigens were observed predominantly in the cytoplasm with stronger fluorescence at the cell surface. Essentially similar findings were obtained in the abortive infections in L cells and BHK cells. The above results suggest that the membrane protein of influenza A virus is present in the nucleus as well as in the cytoplasm of infected cells.
Url:
DOI: 10.1111/j.1348-0421.1977.tb00308.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000352
Links to Exploration step
ISTEX:AB2F3AC58379613B6A3B89BF5EEBD8132DC05983Curation
No country items
Saiji Yoshii<affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation>Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Intracellular Development of Membrane Protein of Influenza Virus</title><author><name sortKey="Maeno, Koichiro" sort="Maeno, Koichiro" uniqKey="Maeno K" first="Koichiro" last="Maeno">Koichiro Maeno</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation wicri:level="1"><mods:affiliation>Correspondence address: Requests for reprints should be addressed to Dr. Koichiro Maeno, Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya, Japan.</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Correspondence address: Requests for reprints should be addressed to Dr. Koichiro Maeno, Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</wicri:regionArea></affiliation></author><author><name sortKey="Yoshii, Saiji" sort="Yoshii, Saiji" uniqKey="Yoshii S" first="Saiji" last="Yoshii">Saiji Yoshii</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Yoshida, Tetsuya" sort="Yoshida, Tetsuya" uniqKey="Yoshida T" first="Tetsuya" last="Yoshida">Tetsuya Yoshida</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Iinuma, Masao" sort="Iinuma, Masao" uniqKey="Iinuma M" first="Masao" last="Iinuma">Masao Iinuma</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Kawamoto, Yasuko" sort="Kawamoto, Yasuko" uniqKey="Kawamoto Y" first="Yasuko" last="Kawamoto">Yasuko Kawamoto</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Matsumoto, Toshisada" sort="Matsumoto, Toshisada" uniqKey="Matsumoto T" first="Toshisada" last="Matsumoto">Toshisada Matsumoto</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AB2F3AC58379613B6A3B89BF5EEBD8132DC05983</idno><date when="1977" year="1977">1977</date><idno type="doi">10.1111/j.1348-0421.1977.tb00308.x</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-59DBK574-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000352</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000352</idno><idno type="wicri:Area/Istex/Curation">000352</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Intracellular Development of Membrane Protein of Influenza Virus</title><author><name sortKey="Maeno, Koichiro" sort="Maeno, Koichiro" uniqKey="Maeno K" first="Koichiro" last="Maeno">Koichiro Maeno</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation>Correspondence address: Requests for reprints should be addressed to Dr. Koichiro Maeno, Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya, Japan.</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Correspondence address: Requests for reprints should be addressed to Dr. Koichiro Maeno, Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</wicri:regionArea></affiliation></author><author><name sortKey="Yoshii, Saiji" sort="Yoshii, Saiji" uniqKey="Yoshii S" first="Saiji" last="Yoshii">Saiji Yoshii</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Yoshida, Tetsuya" sort="Yoshida, Tetsuya" uniqKey="Yoshida T" first="Tetsuya" last="Yoshida">Tetsuya Yoshida</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Iinuma, Masao" sort="Iinuma, Masao" uniqKey="Iinuma M" first="Masao" last="Iinuma">Masao Iinuma</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Kawamoto, Yasuko" sort="Kawamoto, Yasuko" uniqKey="Kawamoto Y" first="Yasuko" last="Kawamoto">Yasuko Kawamoto</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Matsumoto, Toshisada" sort="Matsumoto, Toshisada" uniqKey="Matsumoto T" first="Toshisada" last="Matsumoto">Toshisada Matsumoto</name><affiliation><mods:affiliation>Department of Virology, Cancer Research Institute, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>1st Department of Internal Medicine, Nagoya University School of Medicine, Nagoya</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Bacteriology, Fujitagakuen University School of Medicine, Nagoya</mods:affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Microbiology and Immunology</title><title level="j" type="alt">MICROBIOLOGY AND IMMUNOLOGY</title><idno type="ISSN">0385-5600</idno><idno type="eISSN">1348-0421</idno><imprint><biblScope unit="vol">21</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="427">427</biblScope><biblScope unit="page" to="438">438</biblScope><biblScope unit="page-count">12</biblScope><date type="published" when="1977-08">1977-08</date></imprint><idno type="ISSN">0385-5600</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0385-5600</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Abortive</term><term>Abortive infection</term><term>Academic press</term><term>Antigen</term><term>Antiserum</term><term>Biological activities</term><term>Cell monolayers</term><term>Cell surface</term><term>Clone</term><term>Clone cells</term><term>Continuous lines</term><term>Culture fluids</term><term>Cytoplasmic fluorescence</term><term>Developmental sequence</term><term>Diffuse fluorescence</term><term>Fowl plague virus</term><term>Further incubation</term><term>Immunofluorescent staining</term><term>Infection</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Input multiplicity</term><term>Intracellular</term><term>Intracellular development</term><term>Intracellular sites</term><term>Maeno</term><term>Maintenance medium</term><term>Membrane protein</term><term>Monospecific antiserum</term><term>Newcastle disease virus</term><term>Nonreducing conditions</term><term>Nonstructural proteins</term><term>Nuclear fluorescence</term><term>Nuclear regions</term><term>Paper strips</term><term>Peripheral wells</term><term>Phosphate buffer</term><term>Plasma membrane</term><term>Precipitin lines</term><term>Productive infection</term><term>Protein antigens</term><term>Recent studies</term><term>Room temperature</term><term>Sendai virus</term><term>Single precipitin line</term><term>Stronger fluorescence</term><term>Various times</term><term>Veronal buffer</term><term>Viral</term><term>Virion</term><term>Virology</term><term>Virus</term><term>Whole cell</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">The intracellular development of membrane protein (MP) of influenza A virus was investigated by immunofluorescent staining. Monospecific antiserum was prepared by immunizing rabbits with MP eluted from SDS‐polyacrylamide gels of SDS‐disrupted NWS virions. In the productive infection in clone 1‐5C‐4 cells, MP antigen was first detected over the whole cell at 4 hr after infection, concomitantly with the appearance of hemagglutinin (HA) antigen in the cytoplasm, and bright nuclear fluorescence was then observed. Nucleoprotein (NP) antigen was detected in the nucleus prior to the appearance of fluorescence of MP antigen and thereafter the cytoplasmic fluorescence developed. Late in infection, all of these three antigens were observed predominantly in the cytoplasm with stronger fluorescence at the cell surface. Essentially similar findings were obtained in the abortive infections in L cells and BHK cells. The above results suggest that the membrane protein of influenza A virus is present in the nucleus as well as in the cytoplasm of infected cells.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000352 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000352 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:AB2F3AC58379613B6A3B89BF5EEBD8132DC05983
|texte= Intracellular Development of Membrane Protein of Influenza Virus
}}
| This area was generated with Dilib version V0.6.33. |  |