Thujaplicin–copper chelates inhibit replication of human influenza viruses
Identifieur interne : 000197 ( Istex/Curation ); précédent : 000196; suivant : 000198Thujaplicin–copper chelates inhibit replication of human influenza viruses
Auteurs : Daisei Miyamoto [Japon] ; Yuki Kusagaya [Japon] ; Naoko Endo [Japon] ; Ayako Sometani [Japon] ; Seiji Takeo [Japon] ; Takashi Suzuki [Japon] ; Yaeno Arima [Japon] ; Katsuyuki Nakajima [États-Unis] ; Yasuo Suzuki [Japon]Source :
- Antiviral Research [ 0166-3542 ] ; 1998.
English descriptors
- Teeft :
- Anti6iral, Anti6iral research, Apoptosis, Apoptotic, Assay, Canine kidney, Cell death, Cell growth, Cell viability, Chelate, Chem, Copper chelate, Copper chelates, Copper ions, Copper sulfate, Culture medium, Cytometric, Cytometric analysis, Cytotoxic, Cytotoxic effects, Early phase, Early stage, Hela cells, Host cells, Human apoptosis, Inhibitory effects, Lactate dehydrogenase, Mdck, Mdck cells, Metal chelates, Miyamoto, Morphological changes, Postinfection, Supernatant, Suzuki, Takizawa, Test samples, Thuja plicata, Thujaplicin, Thujaplicin copper chelates, Thujaplicins, Total cells, Viral, Viral infection, Virus, Virus infection, Zinc ions.
Abstract
Abstract: The effects of α-, β- and γ-thujaplicins and six of their metal chelates on human influenza virus-induced apoptosis in Madin–Darby canine kidney (MDCK) cells were examined by DNA fragmentation and flow cytometry. Among the compounds tested, thujaplicin–copper chelates inhibited apoptosis induced in the infected MDCK cells with influenza A/PR/8/34(H1N1), A/Shingapol/1/57(H2N2), A/Aichi/2/68(H3N2) and B/Lee/40 viruses, at concentrations of more than 5 μM. These results indicate that the copper chelates inhibit influenza virus-induced apoptosis and that the inhibitory effects may be independent of influenza virus subtype or types. Furthermore, the copper chelates also inhibited the release of the viruses from the infected MDCK cells during apoptosis. The anti-apoptotic effects of the copper chelates may occur 2–4 h postinfection, suggesting that the copper chelates affect MDCK cells directly in the early stage of influenza virus-induced apoptosis. In this study, we demonstrated that thujaplicin–copper chelates inhibit influenza virus-induced apoptosis of MDCK cells and also inhibit virus replication and release from the infected cells.
Url:
DOI: 10.1016/S0166-3542(98)00034-5
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Thujaplicin–copper chelates inhibit replication of human influenza viruses</title><author><name sortKey="Miyamoto, Daisei" sort="Miyamoto, Daisei" uniqKey="Miyamoto D" first="Daisei" last="Miyamoto">Daisei Miyamoto</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Kusagaya, Yuki" sort="Kusagaya, Yuki" uniqKey="Kusagaya Y" first="Yuki" last="Kusagaya">Yuki Kusagaya</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Endo, Naoko" sort="Endo, Naoko" uniqKey="Endo N" first="Naoko" last="Endo">Naoko Endo</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Sometani, Ayako" sort="Sometani, Ayako" uniqKey="Sometani A" first="Ayako" last="Sometani">Ayako Sometani</name><affiliation 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Nakauti-cho, Misasagi, Yamasina-ku, Kyoto 607</wicri:regionArea></affiliation></author><author><name sortKey="Nakajima, Katsuyuki" sort="Nakajima, Katsuyuki" uniqKey="Nakajima K" first="Katsuyuki" last="Nakajima">Katsuyuki Nakajima</name><affiliation wicri:level="1"><mods:affiliation>Otsuka America Pharmaceutical, Inc., 2440, Research Boulourd, Lock Ville, MD 20850, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Otsuka America Pharmaceutical, Inc., 2440, Research Boulourd, Lock Ville, MD 20850</wicri:regionArea></affiliation></author><author><name sortKey="Yasuo Suzuki" sort="Yasuo Suzuki" uniqKey="Yasuo Suzuki" first="" last="Yasuo Suzuki">Yasuo Suzuki</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:735996364087205C257469F4F7F05747D82AF949</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0166-3542(98)00034-5</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-2DGLS83N-8/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000197</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000197</idno><idno type="wicri:Area/Istex/Curation">000197</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Thujaplicin–copper chelates inhibit replication of human influenza viruses</title><author><name sortKey="Miyamoto, Daisei" sort="Miyamoto, Daisei" uniqKey="Miyamoto D" first="Daisei" last="Miyamoto">Daisei Miyamoto</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Kusagaya, Yuki" sort="Kusagaya, Yuki" uniqKey="Kusagaya Y" first="Yuki" last="Kusagaya">Yuki Kusagaya</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Endo, Naoko" sort="Endo, Naoko" uniqKey="Endo N" first="Naoko" last="Endo">Naoko Endo</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Sometani, Ayako" sort="Sometani, Ayako" uniqKey="Sometani A" first="Ayako" last="Sometani">Ayako Sometani</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Seiji Takeo" sort="Seiji Takeo" uniqKey="Seiji Takeo" first="" last="Seiji Takeo">Seiji Takeo</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Suzuki, Takashi" sort="Suzuki, Takashi" uniqKey="Suzuki T" first="Takashi" last="Suzuki">Takashi Suzuki</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author><author><name sortKey="Arima, Yaeno" sort="Arima, Yaeno" uniqKey="Arima Y" first="Yaeno" last="Arima">Yaeno Arima</name><affiliation wicri:level="1"><mods:affiliation>Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, 5 Nakauti-cho, Misasagi, Yamasina-ku, Kyoto 607, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, 5 Nakauti-cho, Misasagi, Yamasina-ku, Kyoto 607</wicri:regionArea></affiliation></author><author><name sortKey="Nakajima, Katsuyuki" sort="Nakajima, Katsuyuki" uniqKey="Nakajima K" first="Katsuyuki" last="Nakajima">Katsuyuki Nakajima</name><affiliation wicri:level="1"><mods:affiliation>Otsuka America Pharmaceutical, Inc., 2440, Research Boulourd, Lock Ville, MD 20850, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Otsuka America Pharmaceutical, Inc., 2440, Research Boulourd, Lock Ville, MD 20850</wicri:regionArea></affiliation></author><author><name sortKey="Yasuo Suzuki" sort="Yasuo Suzuki" uniqKey="Yasuo Suzuki" first="" last="Yasuo Suzuki">Yasuo Suzuki</name><affiliation wicri:level="1"><mods:affiliation>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Antiviral Research</title><title level="j" type="abbrev">AVR</title><idno type="ISSN">0166-3542</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">39</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="89">89</biblScope><biblScope unit="page" to="100">100</biblScope></imprint><idno type="ISSN">0166-3542</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-3542</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Anti6iral</term><term>Anti6iral research</term><term>Apoptosis</term><term>Apoptotic</term><term>Assay</term><term>Canine kidney</term><term>Cell death</term><term>Cell growth</term><term>Cell viability</term><term>Chelate</term><term>Chem</term><term>Copper chelate</term><term>Copper chelates</term><term>Copper ions</term><term>Copper sulfate</term><term>Culture medium</term><term>Cytometric</term><term>Cytometric analysis</term><term>Cytotoxic</term><term>Cytotoxic effects</term><term>Early phase</term><term>Early stage</term><term>Hela cells</term><term>Host cells</term><term>Human apoptosis</term><term>Inhibitory effects</term><term>Lactate dehydrogenase</term><term>Mdck</term><term>Mdck cells</term><term>Metal chelates</term><term>Miyamoto</term><term>Morphological changes</term><term>Postinfection</term><term>Supernatant</term><term>Suzuki</term><term>Takizawa</term><term>Test samples</term><term>Thuja plicata</term><term>Thujaplicin</term><term>Thujaplicin copper chelates</term><term>Thujaplicins</term><term>Total cells</term><term>Viral</term><term>Viral infection</term><term>Virus</term><term>Virus infection</term><term>Zinc ions</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The effects of α-, β- and γ-thujaplicins and six of their metal chelates on human influenza virus-induced apoptosis in Madin–Darby canine kidney (MDCK) cells were examined by DNA fragmentation and flow cytometry. Among the compounds tested, thujaplicin–copper chelates inhibited apoptosis induced in the infected MDCK cells with influenza A/PR/8/34(H1N1), A/Shingapol/1/57(H2N2), A/Aichi/2/68(H3N2) and B/Lee/40 viruses, at concentrations of more than 5 μM. These results indicate that the copper chelates inhibit influenza virus-induced apoptosis and that the inhibitory effects may be independent of influenza virus subtype or types. Furthermore, the copper chelates also inhibited the release of the viruses from the infected MDCK cells during apoptosis. The anti-apoptotic effects of the copper chelates may occur 2–4 h postinfection, suggesting that the copper chelates affect MDCK cells directly in the early stage of influenza virus-induced apoptosis. In this study, we demonstrated that thujaplicin–copper chelates inhibit influenza virus-induced apoptosis of MDCK cells and also inhibit virus replication and release from the infected cells.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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