Evaluation of H9N2 influenza vaccines in Balb/c mice
Identifieur interne : 000182 ( Istex/Curation ); précédent : 000181; suivant : 000183Evaluation of H9N2 influenza vaccines in Balb/c mice
Auteurs : D. L Major [Royaume-Uni] ; R. W Newman [Royaume-Uni] ; U. Dunleavy [Royaume-Uni] ; A. B Heath [Royaume-Uni] ; K. Ploss [Royaume-Uni] ; J. M Wood [Royaume-Uni]Source :
- International congress series [ 0531-5131 ] ; 2001.
English descriptors
- Teeft :
- Antibody responses, Antigenically, Assay, Challenge strain, Clinical trial vaccines, Dos, Elsevier science, Excretion, Groups immunised, Hong kong, Immune, Immune responses, Immunisation, Immunised, Influenza, Influenza vaccines, Influenza virus, Internal proteins, International congress series, Mdck cells, Mice immunised, Mouse model, Neuraminidase, Partial protection, Reassortant virus, Second immunisation animals, Similar pattern, Single dose, Subunit, Subunit vaccine, Subunit vaccines, Turkey erythrocytes, Vaccine, Vaccine groups, Vaccine strains, Virus, Virus excretion, Virus vaccines, Whole virus, Whole virus vaccine, Whole virus vaccines.
Abstract
Abstract: Background: Following the emergence of H9N2 influenza virus in humans it was important to develop vaccines. Protection studies were performed in mice to assess influenza virus strains for their potential as vaccine strains and to evaluate H9N2 influenza vaccines prepared for use in human clinical trials. Methods: Groups of Balb/c mice were immunised intramuscularly with one or two doses of whole virus or subunit influenza vaccines. Antibody responses were measured by virus neutralisation assay (VN), haemagglutination inhibition assay (HI) and neuraminidase inhibition assay (NI). Fourteen days after the second immunisation animals were challenged with a live human H9N2 influenza virus. Virus excretion in nasal washes was monitored daily for 10 days following challenge. Results: Good HI responses to all but the subunit vaccine were detected. VN titres correlated well with the HI data. NI responses were detectable in all groups of mice except those that had received a single dose of subunit vaccine. Following challenge virus was not recovered from any animal immunised with two doses of whole virus vaccine prepared from human H9N2 virus. Virus excretion in the single dose group and groups immunised with whole virus vaccine prepared from swine H9N2 virus was significantly reduced. Virus excretion in the groups immunised with subunit H9N2 vaccine or H3N2 vaccine was similar to that in the controls. Conclusions: Whole virus H9N2 vaccine is more immunogenic and protective in mice than subunit vaccine. The mouse model is useful for preclinical evaluation of candidate pandemic influenza vaccines although it requires clinical validation.
Url:
DOI: 10.1016/S0531-5131(01)00357-0
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Dunleavy</name><affiliation wicri:level="1"><mods:affiliation>National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QC, UK</mods:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QC</wicri:regionArea></affiliation></author><author><name sortKey="Heath, A B" sort="Heath, A B" uniqKey="Heath A" first="A. B" last="Heath">A. 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M" last="Wood">J. M Wood</name><affiliation wicri:level="1"><mods:affiliation>National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QC, UK</mods:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QC</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">International congress series</title><title level="j" type="abbrev">ICS</title><idno type="ISSN">0531-5131</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">1219</biblScope><biblScope unit="supplement">C</biblScope><biblScope unit="page" from="789">789</biblScope><biblScope unit="page" to="794">794</biblScope></imprint><idno type="ISSN">0531-5131</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0531-5131</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antibody responses</term><term>Antigenically</term><term>Assay</term><term>Challenge strain</term><term>Clinical trial vaccines</term><term>Dos</term><term>Elsevier science</term><term>Excretion</term><term>Groups immunised</term><term>Hong kong</term><term>Immune</term><term>Immune responses</term><term>Immunisation</term><term>Immunised</term><term>Influenza</term><term>Influenza vaccines</term><term>Influenza virus</term><term>Internal proteins</term><term>International congress series</term><term>Mdck cells</term><term>Mice immunised</term><term>Mouse model</term><term>Neuraminidase</term><term>Partial protection</term><term>Reassortant virus</term><term>Second immunisation animals</term><term>Similar pattern</term><term>Single dose</term><term>Subunit</term><term>Subunit vaccine</term><term>Subunit vaccines</term><term>Turkey erythrocytes</term><term>Vaccine</term><term>Vaccine groups</term><term>Vaccine strains</term><term>Virus</term><term>Virus excretion</term><term>Virus vaccines</term><term>Whole virus</term><term>Whole virus vaccine</term><term>Whole virus vaccines</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Background: Following the emergence of H9N2 influenza virus in humans it was important to develop vaccines. Protection studies were performed in mice to assess influenza virus strains for their potential as vaccine strains and to evaluate H9N2 influenza vaccines prepared for use in human clinical trials. Methods: Groups of Balb/c mice were immunised intramuscularly with one or two doses of whole virus or subunit influenza vaccines. Antibody responses were measured by virus neutralisation assay (VN), haemagglutination inhibition assay (HI) and neuraminidase inhibition assay (NI). Fourteen days after the second immunisation animals were challenged with a live human H9N2 influenza virus. Virus excretion in nasal washes was monitored daily for 10 days following challenge. Results: Good HI responses to all but the subunit vaccine were detected. VN titres correlated well with the HI data. NI responses were detectable in all groups of mice except those that had received a single dose of subunit vaccine. Following challenge virus was not recovered from any animal immunised with two doses of whole virus vaccine prepared from human H9N2 virus. Virus excretion in the single dose group and groups immunised with whole virus vaccine prepared from swine H9N2 virus was significantly reduced. Virus excretion in the groups immunised with subunit H9N2 vaccine or H3N2 vaccine was similar to that in the controls. Conclusions: Whole virus H9N2 vaccine is more immunogenic and protective in mice than subunit vaccine. The mouse model is useful for preclinical evaluation of candidate pandemic influenza vaccines although it requires clinical validation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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