The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit
Identifieur interne : 001606 ( Istex/Corpus ); précédent : 001605; suivant : 001607The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit
Auteurs : Nadia Naffakh ; Pascale Massin ; Sylvie Van Der WerfSource :
- Virology [ 0042-6822 ] ; 2001.
English descriptors
- KwdEn :
- Teeft :
- Amino, Amino acid, Amino acid substitutions, Amino acids, Assay, Avian, Avian origin, Cdna, Cell extracts, Cells cotransfected, Cells transfected, Coexpressed proteins, Control phmg plasmid, Encoding, Experiment representative, Galactosidase, Galactosidase levels, Galactosidase proteolysis, Galactosidase proteolyzed, Influenza, Influenza replication, Influenza virus, Influenza viruses, Krug, Molecular dynamics, Mrna, Mutant, Naffakh, Optical scanner, Ortin, Perales, Phmg, Phmg plasmid, Plasmid, Polyclonal serum, Polymerase, Polymerase activity, Posttransfection, Present study, Proteolysis, Proteolytic, Proteolytic activity, Proteolyzed, Replication, Replication activity, Reporter plasmid, Rna, Substitution, Subunit, Transcription, Transfected, Transfected cells, Virol, Western blot analysis.
Abstract
Abstract: The PA subunit of the influenza virus polymerase has been shown to induce degradation of coexpressed proteins, but its role in the replication activity of the polymerase is not fully understood. Here, PA proteins derived from several influenza A viruses were examined at 37 and 33°C for both the level of proteolysis they induced and the efficiency with which they ensured transcription/replication of a viral-like RNA within a polymerase complex reconstituted in vivo from cloned cDNAs. Two mutants of A/Victoria/3/75 PA showed a decreased ability to induce proteolysis as compared to the wild-type PA, but still appeared to be as active as the wild-type protein with respect to the polymerase activity. Furthermore, we observed that the ability of PR8-PA to induce proteolysis was severely impaired at 33°C as compared to 37°C, while the efficiency with which the PR8-derived polymerase complex ensured transcription/replication of the viral-like RNA was similar at both temperatures. Taken together, our observations suggest that the transcription/replication activity of the polymerase of influenza A viruses is not correlated with the level of proteolysis induced by the PA subunit.
Url:
DOI: 10.1006/viro.2001.0956
Links to Exploration step
ISTEX:1FDEC5165A79FB0E12F3968DC1930157F5E8559DLe document en format XML
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uniqKey="Naffakh N" first="Nadia" last="Naffakh">Nadia Naffakh</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Massin, Pascale" sort="Massin, Pascale" uniqKey="Massin P" first="Pascale" last="Massin">Pascale Massin</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" 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plasmid</term><term>Encoding</term><term>Experiment representative</term><term>Galactosidase</term><term>Galactosidase levels</term><term>Galactosidase proteolysis</term><term>Galactosidase proteolyzed</term><term>Influenza</term><term>Influenza replication</term><term>Influenza virus</term><term>Influenza viruses</term><term>Krug</term><term>Molecular dynamics</term><term>Mrna</term><term>Mutant</term><term>Naffakh</term><term>Optical scanner</term><term>Ortin</term><term>Perales</term><term>Phmg</term><term>Phmg plasmid</term><term>Plasmid</term><term>Polyclonal serum</term><term>Polymerase</term><term>Polymerase activity</term><term>Posttransfection</term><term>Present study</term><term>Proteolysis</term><term>Proteolytic</term><term>Proteolytic activity</term><term>Proteolyzed</term><term>Replication</term><term>Replication activity</term><term>Reporter plasmid</term><term>Rna</term><term>Substitution</term><term>Subunit</term><term>Transcription</term><term>Transfected</term><term>Transfected cells</term><term>Virol</term><term>Western blot analysis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The PA subunit of the influenza virus polymerase has been shown to induce degradation of coexpressed proteins, but its role in the replication activity of the polymerase is not fully understood. Here, PA proteins derived from several influenza A viruses were examined at 37 and 33°C for both the level of proteolysis they induced and the efficiency with which they ensured transcription/replication of a viral-like RNA within a polymerase complex reconstituted in vivo from cloned cDNAs. Two mutants of A/Victoria/3/75 PA showed a decreased ability to induce proteolysis as compared to the wild-type PA, but still appeared to be as active as the wild-type protein with respect to the polymerase activity. Furthermore, we observed that the ability of PR8-PA to induce proteolysis was severely impaired at 33°C as compared to 37°C, while the efficiency with which the PR8-derived polymerase complex ensured transcription/replication of the viral-like RNA was similar at both temperatures. Taken together, our observations suggest that the transcription/replication activity of the polymerase of influenza A viruses is not correlated with the level of proteolysis induced by the PA subunit.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>plasmid</json:string><json:string>polymerase</json:string><json:string>mutant</json:string><json:string>galactosidase</json:string><json:string>subunit</json:string><json:string>transfected</json:string><json:string>mrna</json:string><json:string>proteolysis</json:string><json:string>virol</json:string><json:string>phmg</json:string><json:string>posttransfection</json:string><json:string>influenza</json:string><json:string>replication</json:string><json:string>influenza virus</json:string><json:string>avian</json:string><json:string>perales</json:string><json:string>encoding</json:string><json:string>cdna</json:string><json:string>ortin</json:string><json:string>proteolytic activity</json:string><json:string>rna</json:string><json:string>naffakh</json:string><json:string>galactosidase proteolysis</json:string><json:string>amino acids</json:string><json:string>amino</json:string><json:string>proteolyzed</json:string><json:string>replication activity</json:string><json:string>krug</json:string><json:string>avian origin</json:string><json:string>polymerase activity</json:string><json:string>molecular dynamics</json:string><json:string>galactosidase proteolyzed</json:string><json:string>proteolytic</json:string><json:string>influenza replication</json:string><json:string>transfected cells</json:string><json:string>optical scanner</json:string><json:string>western blot analysis</json:string><json:string>cell extracts</json:string><json:string>experiment representative</json:string><json:string>assay</json:string><json:string>cells transfected</json:string><json:string>influenza viruses</json:string><json:string>amino acid substitutions</json:string><json:string>amino acid</json:string><json:string>present study</json:string><json:string>control phmg plasmid</json:string><json:string>polyclonal serum</json:string><json:string>galactosidase levels</json:string><json:string>reporter plasmid</json:string><json:string>phmg plasmid</json:string><json:string>coexpressed proteins</json:string><json:string>cells cotransfected</json:string><json:string>substitution</json:string><json:string>transcription</json:string></teeft></keywords><author><json:item><name>Nadia Naffakh</name><affiliations><json:string>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</json:string></affiliations></json:item><json:item><name>Pascale Massin</name><affiliations><json:string>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</json:string></affiliations></json:item><json:item><name>Sylvie van der Werf</name><affiliations><json:string>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>influenza virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PA protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>replication</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>proteolysis</value></json:item></subject><arkIstex>ark:/67375/6H6-W3XCQQX4-Q</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The PA subunit of the influenza virus polymerase has been shown to induce degradation of coexpressed proteins, but its role in the replication activity of the polymerase is not fully understood. Here, PA proteins derived from several influenza A viruses were examined at 37 and 33°C for both the level of proteolysis they induced and the efficiency with which they ensured transcription/replication of a viral-like RNA within a polymerase complex reconstituted in vivo from cloned cDNAs. Two mutants of A/Victoria/3/75 PA showed a decreased ability to induce proteolysis as compared to the wild-type PA, but still appeared to be as active as the wild-type protein with respect to the polymerase activity. Furthermore, we observed that the ability of PR8-PA to induce proteolysis was severely impaired at 33°C as compared to 37°C, while the efficiency with which the PR8-derived polymerase complex ensured transcription/replication of the viral-like RNA was similar at both temperatures. Taken together, our observations suggest that the transcription/replication activity of the polymerase of influenza A viruses is not correlated with the level of proteolysis induced by the PA subunit.</abstract><qualityIndicators><score>9.172</score><pdfWordCount>6380</pdfWordCount><pdfCharCount>38560</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>534 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>181</abstractWordCount><abstractCharCount>1197</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit</title><pmid><json:string>11437659</json:string></pmid><pii><json:string>S0042-6822(01)90956-0</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>2001</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0015-3</json:string></pii><volume>285</volume><issue>2</issue><pages><first>244</first><last>252</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1997</json:string><json:string>2001</json:string></date><geogName></geogName><orgName><json:string>Medical Center</json:string><json:string>Ministere</json:string></orgName><orgName_funder><json:string>Ministere</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Paris Cedex</json:string><json:string>Hong Kong</json:string><json:string>ELISA Kit</json:string><json:string>J. Pavlovic</json:string><json:string>C. Analysis</json:string><json:string>A. Portela</json:string><json:string>Rita Medeiros</json:string><json:string>Pascale Massin</json:string><json:string>C. Estimation</json:string><json:string>Roux</json:string><json:string>To</json:string><json:string>Sylvie van der Werf</json:string><json:string>J. Ortin</json:string><json:string>Marco Vignuzzi</json:string><json:string>P. Palese</json:string></persName><placeName><json:string>COS</json:string><json:string>Korea</json:string><json:string>Hong Kong</json:string><json:string>Madrid</json:string><json:string>Puerto Rico</json:string><json:string>Rostock</json:string><json:string>France</json:string><json:string>Spain</json:string><json:string>Zurich</json:string></placeName><ref_url><json:string>http://www.idealibrary.com</json:string></ref_url><ref_bibl><json:string>Pleschka et al. (1996)</json:string><json:string>Perales et al., 2000</json:string><json:string>Sanz-Ezquerro et al., 1998</json:string><json:string>Nakagawa et al., 1995</json:string><json:string>Pleschka et al., 1996</json:string><json:string>Toyoda et al., 1996</json:string><json:string>Huang et al., 1990</json:string><json:string>Digard et al., 1989</json:string><json:string>Perales and Ortin, 1997</json:string><json:string>Hay, 1998</json:string><json:string>Sanz-Ezquerro et al., 1996</json:string><json:string>Sanz-Ezquerro et al. (1996)</json:string><json:string>Poon et al., 1999</json:string><json:string>Claas et al., 1998</json:string><json:string>Perales et al.</json:string><json:string>Gonzalez et al., 1996</json:string><json:string>Perales et al. (2000)</json:string><json:string>Luo et al., 1991</json:string><json:string>Zurcher et al., 1996</json:string><json:string>Sanz-Ezquerro et al., 1995</json:string><json:string>Plotch et al., 1981</json:string><json:string>Sanz-Ezquerro et al.</json:string><json:string>reviewed in Mahy, 1983</json:string><json:string>Biswas and Nayak, 1994</json:string><json:string>Blaas et al., 1982</json:string><json:string>Shi et al., 1996, 1995</json:string><json:string>Pogulis et al., 1996</json:string><json:string>Subbarao et al., 1998</json:string><json:string>Biswas et al., 1998</json:string><json:string>Lamb and Krug, 1996</json:string><json:string>Braam et al., 1983</json:string><json:string>SanzEzquerro et al., 1996</json:string><json:string>Blok et al., 1996</json:string><json:string>Bouloy et al., 1978</json:string><json:string>Naffakh et al., 2000</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-W3XCQQX4-Q</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1006/viro.2001.0956</json:string></doi><id>1FDEC5165A79FB0E12F3968DC1930157F5E8559D</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W3XCQQX4-Q/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W3XCQQX4-Q/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-W3XCQQX4-Q/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©2001 Academic Press</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>2001</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit</title><author xml:id="author-0000"><persName><forename type="first">Nadia</forename><surname>Naffakh</surname></persName><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Pascale</forename><surname>Massin</surname></persName><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Sylvie</forename><surname>van der Werf</surname></persName><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</affiliation></author><idno type="istex">1FDEC5165A79FB0E12F3968DC1930157F5E8559D</idno><idno type="ark">ark:/67375/6H6-W3XCQQX4-Q</idno><idno type="DOI">10.1006/viro.2001.0956</idno><idno type="PII">S0042-6822(01)90956-0</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0015-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">285</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="244">244</biblScope><biblScope unit="page" to="252">252</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The PA subunit of the influenza virus polymerase has been shown to induce degradation of coexpressed proteins, but its role in the replication activity of the polymerase is not fully understood. Here, PA proteins derived from several influenza A viruses were examined at 37 and 33°C for both the level of proteolysis they induced and the efficiency with which they ensured transcription/replication of a viral-like RNA within a polymerase complex reconstituted in vivo from cloned cDNAs. Two mutants of A/Victoria/3/75 PA showed a decreased ability to induce proteolysis as compared to the wild-type PA, but still appeared to be as active as the wild-type protein with respect to the polymerase activity. Furthermore, we observed that the ability of PR8-PA to induce proteolysis was severely impaired at 33°C as compared to 37°C, while the efficiency with which the PR8-derived polymerase complex ensured transcription/replication of the viral-like RNA was similar at both temperatures. Taken together, our observations suggest that the transcription/replication activity of the polymerase of influenza A viruses is not correlated with the level of proteolysis induced by the PA subunit.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>influenza virus</term></item><item><term>PA protein</term></item><item><term>replication</term></item><item><term>proteolysis</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001-02-13">Modified</change><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W3XCQQX4-Q/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YVIRO</jid><aid>90956</aid><ce:pii>S0042-6822(01)90956-0</ce:pii><ce:doi>10.1006/viro.2001.0956</ce:doi><ce:copyright type="full-transfer" year="2001">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit</ce:title><ce:author-group><ce:author><ce:given-name>Nadia</ce:given-name><ce:surname>Naffakh</ce:surname></ce:author><ce:author><ce:given-name>Pascale</ce:given-name><ce:surname>Massin</ce:surname></ce:author><ce:author><ce:given-name>Sylvie</ce:given-name><ce:surname>van der Werf</ce:surname><ce:cross-ref refid="FN1">1<ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>To whom correspondence and reprint requests should be addressed at Unité de Génétique Moléculaire des Virus Respiratoires, URA CNRS 1966, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Fax : +33-1-40-61-32-41. E-mail: svdwerf@pasteur.fr.</ce:note-para></ce:footnote></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="2" month="1" year="2001"></ce:date-received><ce:date-revised day="13" month="2" year="2001"></ce:date-revised><ce:date-accepted day="13" month="4" year="2001"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The PA subunit of the influenza virus polymerase has been shown to induce degradation of coexpressed proteins, but its role in the replication activity of the polymerase is not fully understood. Here, PA proteins derived from several influenza A viruses were examined at 37 and 33°C for both the level of proteolysis they induced and the efficiency with which they ensured transcription/replication of a viral-like RNA within a polymerase complex reconstituted <ce:italic>in vivo</ce:italic> from cloned cDNAs. Two mutants of A/Victoria/3/75 PA showed a decreased ability to induce proteolysis as compared to the wild-type PA, but still appeared to be as active as the wild-type protein with respect to the polymerase activity. Furthermore, we observed that the ability of PR8-PA to induce proteolysis was severely impaired at 33°C as compared to 37°C, while the efficiency with which the PR8-derived polymerase complex ensured transcription/replication of the viral-like RNA was similar at both temperatures. Taken together, our observations suggest that the transcription/replication activity of the polymerase of influenza A viruses is not correlated with the level of proteolysis induced by the PA subunit.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>influenza virus</ce:text></ce:keyword><ce:keyword><ce:text>PA protein</ce:text></ce:keyword><ce:keyword><ce:text>replication</ce:text></ce:keyword><ce:keyword><ce:text>proteolysis</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>The Transcription/Replication Activity of the Polymerase of Influenza A Viruses Is Not Correlated with the Level of Proteolysis Induced by the PA Subunit</title></titleInfo><name type="personal"><namePart type="given">Nadia</namePart><namePart type="family">Naffakh</namePart><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pascale</namePart><namePart type="family">Massin</namePart><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sylvie</namePart><namePart type="family">van der Werf</namePart><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, Paris, France</affiliation><description>To whom correspondence and reprint requests should be addressed at Unité de Génétique Moléculaire des Virus Respiratoires, URA CNRS 1966, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Fax : +33-1-40-61-32-41. E-mail: svdwerf@pasteur.fr.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><dateModified encoding="w3cdtf">2001-02-13</dateModified><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The PA subunit of the influenza virus polymerase has been shown to induce degradation of coexpressed proteins, but its role in the replication activity of the polymerase is not fully understood. Here, PA proteins derived from several influenza A viruses were examined at 37 and 33°C for both the level of proteolysis they induced and the efficiency with which they ensured transcription/replication of a viral-like RNA within a polymerase complex reconstituted in vivo from cloned cDNAs. Two mutants of A/Victoria/3/75 PA showed a decreased ability to induce proteolysis as compared to the wild-type PA, but still appeared to be as active as the wild-type protein with respect to the polymerase activity. Furthermore, we observed that the ability of PR8-PA to induce proteolysis was severely impaired at 33°C as compared to 37°C, while the efficiency with which the PR8-derived polymerase complex ensured transcription/replication of the viral-like RNA was similar at both temperatures. Taken together, our observations suggest that the transcription/replication activity of the polymerase of influenza A viruses is not correlated with the level of proteolysis induced by the PA subunit.</abstract><note type="content">Section title: Regular Article</note><subject lang="en"><genre>Keywords</genre><topic>influenza virus</topic><topic>PA protein</topic><topic>replication</topic><topic>proteolysis</topic></subject><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0015-3</identifier><part><date>2001</date><detail type="volume"><number>285</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>177</start><end>377</end></extent><extent unit="pages"><start>244</start><end>252</end></extent></part></relatedItem><identifier type="istex">1FDEC5165A79FB0E12F3968DC1930157F5E8559D</identifier><identifier type="ark">ark:/67375/6H6-W3XCQQX4-Q</identifier><identifier type="DOI">10.1006/viro.2001.0956</identifier><identifier type="PII">S0042-6822(01)90956-0</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©2001</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W3XCQQX4-Q/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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