Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60- ca
Identifieur interne : 001312 ( Istex/Corpus ); précédent : 001311; suivant : 001313Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60- ca
Auteurs : G. A. Tannock ; Deborah A. Bryce ; Judith A. PaulSource :
- Vaccine [ 0264-410X ] ; 1985.
English descriptors
- KwdEn :
- Teeft :
- Academic press, Allantoic, Allantoic cavity, Allantoicgrown preparations, Assay, Attenuated, Attenuated recombinants, Bead suspension, Bench centrifuge, Bovine kidney epithelial cells, Cell concentration, Cell culture, Cell growth, Cell suspensions, Cell volume, Cell volumes, Chick, Chick embryo, Chick embryos, Chicken embryo kidney, Chicken kidney, Chicken kidneys, Comparable yields, Culture fluid, Culture medium, Diploid cell line, Efficient growth, Embryo, Embryonated eggs, Growth curves, Higher yields, Identical doses, Immunogenicity, Infectious unit, Infectivity titres, Influenza, Influenza vaccines, Influenza virus, Influenza viruses, Initial suspension, Intranasal route, Kidney, Kidney pair, Large numbers, Less protein, Liquid nitrogen, Maintenance medium, Microcarrier, Microcarrier cultures, Microcarrier suspensions, Microcarriers, Monkey kidney cells, Monolayer, Monolayer cultures, Monolayers, Nuclei counts, Overnight trypsinization, Parental virus, Petri, Petri dish cultures, Plaque assays, Primary cells, Relative immunogenicity, Roller, Roller cultures, Same dilution, Separate experiments, Sodium bicarbonate, Standard error, Supernatant, Supernatant fluid, Support growth, Tannock, Total yields, Trypsin, Trypsin solution, Trypsinization, Trypsinized, Vaccine, Various intervals, Virus, Virus assay, Whole embryos.
Abstract
Primary chicken kidney (CK) and chicken embryo kidney (CEK) cells were evaluated as possible substrates for growth of the cold-adapted attenuated influenza vaccine master strain A/Ann Arbor/6/60 (A/AA/6/60-ca). Yields of 106–107 TCID50 per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-ca was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. Allantoic-grown preparations of A/AA/6/60-ca contained a lower protein concentration per infectious unit than those grown in CEK
Url:
DOI: 10.1016/S0264-410X(85)90245-2
Links to Exploration step
ISTEX:BCB81B418980492394F1F1293BD5DBA8B6363938Le document en format XML
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Yields of 106–107 TCID50 per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-ca was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. 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Tannock</name><affiliations><json:string>Faculty of Medicine, The University of Newcastle, New South Wales 2308, Australia</json:string></affiliations></json:item><json:item><name>Deborah A. Bryce</name><affiliations><json:string>Faculty of Medicine, The University of Newcastle, New South Wales 2308, Australia</json:string></affiliations></json:item><json:item><name>Judith A. 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Yields of 106–107 TCID50 per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-ca was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. Allantoic-grown preparations of A/AA/6/60-ca contained a lower protein concentration per infectious unit than those grown in CEK</abstract><qualityIndicators><score>8.706</score><pdfWordCount>4630</pdfWordCount><pdfCharCount>27215</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>562 x 836 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>173</abstractWordCount><abstractCharCount>1172</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60- ca</title><pii><json:string>S0264-410X(85)90245-2</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Negative strand viruses</title><language><json:string>unknown</json:string></language><volume>vol 2</volume><pages><first>755</first></pages><editor><json:item><name>B.W.J. 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Bryce</json:string><json:string>Judith A. Paul</json:string><json:string>C. 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Yields of 106–107 TCID50 per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-ca was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. Allantoic-grown preparations of A/AA/6/60-ca contained a lower protein concentration per infectious unit than those grown in CEK</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>Viruses</term></item><item><term>influenza</term></item><item><term>cell culture</term></item><item><term>attenuated virus</term></item><item><term>large-scale growth</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1985">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-2PQ10N7T-8/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="fla"><item-info><jid>JVAC</jid><aid>85902452</aid><ce:pii>S0264-410X(85)90245-2</ce:pii><ce:doi>10.1016/S0264-410X(85)90245-2</ce:doi><ce:copyright type="unknown" year="1985">Butterworth & Co (Publishers) Ltd.</ce:copyright></item-info><head><ce:dochead><ce:textfn>Paper</ce:textfn></ce:dochead><ce:title>Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60-<ce:italic>ca</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>G.A.</ce:given-name><ce:surname>Tannock</ce:surname></ce:author><ce:author><ce:given-name>Deborah A.</ce:given-name><ce:surname>Bryce</ce:surname></ce:author><ce:author><ce:given-name>Judith A.</ce:given-name><ce:surname>Paul</ce:surname></ce:author><ce:affiliation id="aff1"><ce:textfn>Faculty of Medicine, The University of Newcastle, New South Wales 2308, Australia</ce:textfn></ce:affiliation></ce:author-group><ce:abstract id="ab1" class="author" xml:lang="en"><ce:abstract-sec><ce:simple-para>Primary chicken kidney (CK) and chicken embryo kidney (CEK) cells were evaluated as possible substrates for growth of the cold-adapted attenuated influenza vaccine master strain A/Ann Arbor/6/60 (A/AA/6/60-<ce:italic>ca</ce:italic>). Yields of 10<ce:sup loc="post">6</ce:sup>–10<ce:sup loc="post">7</ce:sup> TCID<ce:inf loc="post">50</ce:inf> per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-<ce:italic>ca</ce:italic> was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. Allantoic-grown preparations of A/AA/6/60-<ce:italic>ca</ce:italic> contained a lower protein concentration per infectious unit than those grown in CEK</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords xml:lang="en"><ce:keyword><ce:text>Viruses</ce:text></ce:keyword><ce:keyword><ce:text>influenza</ce:text></ce:keyword><ce:keyword><ce:text>cell culture</ce:text></ce:keyword><ce:keyword><ce:text>attenuated virus</ce:text></ce:keyword><ce:keyword><ce:text>large-scale growth</ce:text></ce:keyword></ce:keywords></head><tail><ce:bibliography><ce:section-title>References</ce:section-title><ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>1</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Ada</ce:surname><ce:given-name>G.L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Vaccination against influenza 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II. Evaluation in adult volunteers</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Infect. Dis.</sb:maintitle></sb:title><sb:volume-nr>142</sb:volume-nr></sb:series><sb:date>1980</sb:date></sb:issue><sb:pages><sb:first-page>857</sb:first-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60- ca</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60-</title></titleInfo><name type="personal"><namePart type="given">G.A.</namePart><namePart type="family">Tannock</namePart><affiliation>Faculty of Medicine, The University of Newcastle, New South Wales 2308, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Deborah A.</namePart><namePart type="family">Bryce</namePart><affiliation>Faculty of Medicine, The University of Newcastle, New South Wales 2308, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Judith A.</namePart><namePart type="family">Paul</namePart><affiliation>Faculty of Medicine, The University of Newcastle, New South Wales 2308, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued><copyrightDate encoding="w3cdtf">1985</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Primary chicken kidney (CK) and chicken embryo kidney (CEK) cells were evaluated as possible substrates for growth of the cold-adapted attenuated influenza vaccine master strain A/Ann Arbor/6/60 (A/AA/6/60-ca). Yields of 106–107 TCID50 per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-ca was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. Allantoic-grown preparations of A/AA/6/60-ca contained a lower protein concentration per infectious unit than those grown in CEK</abstract><note type="content">Section title: Paper</note><subject lang="en"><topic>Viruses</topic><topic>influenza</topic><topic>cell culture</topic><topic>attenuated virus</topic><topic>large-scale growth</topic></subject><relatedItem type="host"><titleInfo><title>Vaccine</title></titleInfo><titleInfo type="abbreviated"><title>JVAC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued></originInfo><identifier type="ISSN">0264-410X</identifier><identifier type="PII">S0264-410X(00)X0289-7</identifier><part><date>1985</date><detail type="volume"><number>3</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue-pages"><start>282</start><end>352</end></extent><extent unit="pages"><start>333</start><end>339</end></extent></part></relatedItem><identifier type="istex">BCB81B418980492394F1F1293BD5DBA8B6363938</identifier><identifier type="ark">ark:/67375/6H6-2PQ10N7T-8</identifier><identifier type="DOI">10.1016/S0264-410X(85)90245-2</identifier><identifier type="PII">S0264-410X(85)90245-2</identifier><identifier type="ArticleID">85902452</identifier><accessCondition type="use and reproduction" contentType="copyright">©1985 Butterworth & Co (Publishers) Ltd.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Butterworth & Co (Publishers) Ltd., ©1985</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-2PQ10N7T-8/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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