Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test
Identifieur interne : 001178 ( Istex/Corpus ); précédent : 001177; suivant : 001179Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test
Auteurs : J. J. Chomel ; D. Thouvenot ; M. Onno ; C. Kaiser ; J. M. Gourreau ; M. AymardSource :
- Journal of Virological Methods [ 0166-0934 ] ; 1989.
English descriptors
- Teeft :
- Cell cultures, Clin, Clinical specimens, Elisa, Embryonated, Embryonated eggs, Haemagglutinin, Harmon, Immunocapture, Immunocapture elisa, Immunocapture elisa test, Immunofluorescence, Indirect immunofluorescence, Indirect immunofluorescence test, Influenza, Influenza diagnosis, Influenza infection, Microbial, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Nasal swabs, Nasopharyngeal, Nasopharyngeal secretions, Porcine, Porcine strains, Rabbit antiserum, Rapid diagnosis, Swab, Virus isolation.
Abstract
Abstract: An immunocapture ELISA test for the diagnosis of human and animal influenza A and/or B is described. A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. It also permitted diagnosis in frozen samples (− 20°C) or in infected LLCMK2 cells mixed with uninfected nasopharyngeal cells and kept at 20°C for one week.This test can be carried out in 3 h.
Url:
DOI: 10.1016/0166-0934(89)90102-X
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ISTEX:530BE6557D7CEA2C14275DEBC1B1569F1801A53DLe document en format XML
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Gourreau</name><affiliation><mods:affiliation>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</mods:affiliation></affiliation></author><author><name sortKey="Aymard, M" sort="Aymard, M" uniqKey="Aymard M" first="M." last="Aymard">M. Aymard</name><affiliation><mods:affiliation>Laboratoire de Virologie. 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A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. 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Chomel</name><affiliations><json:string>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</json:string></affiliations></json:item><json:item><name>D. Thouvenot</name><affiliations><json:string>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</json:string></affiliations></json:item><json:item><name>M. Onno</name><affiliations><json:string>Station de Pathologie Porcine, Ploufragan, France</json:string></affiliations></json:item><json:item><name>C. Kaiser</name><affiliations><json:string>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</json:string></affiliations></json:item><json:item><name>J.M. Gourreau</name><affiliations><json:string>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</json:string></affiliations></json:item><json:item><name>M. Aymard</name><affiliations><json:string>Laboratoire de Virologie. 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A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. It also permitted diagnosis in frozen samples (− 20°C) or in infected LLCMK2 cells mixed with uninfected nasopharyngeal cells and kept at 20°C for one week.This test can be carried out in 3 h.</abstract><qualityIndicators><score>6.691</score><pdfWordCount>3251</pdfWordCount><pdfCharCount>20678</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>432 x 648 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>120</abstractWordCount><abstractCharCount>792</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test</title><pmid><json:string>2674180</json:string></pmid><pii><json:string>0166-0934(89)90102-X</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Applications of immunofluorescence</title><language><json:string>unknown</json:string></language></serie><host><title>Journal of Virological Methods</title><language><json:string>unknown</json:string></language><publicationDate>1989</publicationDate><issn><json:string>0166-0934</json:string></issn><pii><json:string>S0166-0934(00)X0143-7</json:string></pii><volume>25</volume><issue>1</issue><pages><first>81</first><last>91</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1974</json:string><json:string>10s</json:string><json:string>1987</json:string><json:string>1988</json:string><json:string>1989</json:string><json:string>1985</json:string></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>J.J. 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(1984)</json:string><json:string>Chomel et al., 1986</json:string><json:string>Frank et al., 1979</json:string><json:string>Advanced Laboratory Techniques for Influenza Diagnosis, 1975</json:string><json:string>Minnich and Ray (1980)</json:string><json:string>Harmon and Pawlik (1982)</json:string><json:string>Harmon et al. (1980)</json:string><json:string>Gardner and McQuillin (1974)</json:string><json:string>Gardner and McQuillin, 1974</json:string><json:string>Cook et al. (1988)</json:string><json:string>March-April 1988</json:string><json:string>Coonrod et al., 1984</json:string><json:string>Harmon et al., 1983</json:string><json:string>Sarkkinen et al., 1981</json:string><json:string>Aymard and Sohier, 1975</json:string><json:string>McQuillin et al., 1985</json:string><json:string>Hayden and Monto, 1986</json:string><json:string>Liu, 1956</json:string><json:string>Walls et al., 1986</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-7QBPG8ZB-5</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string><json:string>2 - biotechnology & applied microbiology</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1989</publicationDate><copyrightDate>1989</copyrightDate><doi><json:string>10.1016/0166-0934(89)90102-X</json:string></doi><id>530BE6557D7CEA2C14275DEBC1B1569F1801A53D</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-7QBPG8ZB-5/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-7QBPG8ZB-5/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-7QBPG8ZB-5/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1989</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test</title><author xml:id="author-0000"><persName><forename type="first">J.J.</forename><surname>Chomel</surname></persName><note type="correspondence"><p>Correspondence to: Dr. J.J. Chomel, Laboratoire de Virologie, Faculté de Médecine, 8, avenue Rockefeller, 69373 Lyon Cedex, France.</p></note><affiliation>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">D.</forename><surname>Thouvenot</surname></persName><affiliation>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">M.</forename><surname>Onno</surname></persName><affiliation>Station de Pathologie Porcine, Ploufragan, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">C.</forename><surname>Kaiser</surname></persName><affiliation>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">J.M.</forename><surname>Gourreau</surname></persName><affiliation>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</affiliation></author><author xml:id="author-0005"><persName><forename type="first">M.</forename><surname>Aymard</surname></persName><affiliation>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</affiliation></author><idno type="istex">530BE6557D7CEA2C14275DEBC1B1569F1801A53D</idno><idno type="ark">ark:/67375/6H6-7QBPG8ZB-5</idno><idno type="DOI">10.1016/0166-0934(89)90102-X</idno><idno type="PII">0166-0934(89)90102-X</idno></analytic><monogr><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="pISSN">0166-0934</idno><idno type="PII">S0166-0934(00)X0143-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989"></date><biblScope unit="volume">25</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="81">81</biblScope><biblScope unit="page" to="91">91</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1989</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: An immunocapture ELISA test for the diagnosis of human and animal influenza A and/or B is described. A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. It also permitted diagnosis in frozen samples (− 20°C) or in infected LLCMK2 cells mixed with uninfected nasopharyngeal cells and kept at 20°C for one week.This test can be carried out in 3 h.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Influenza</term></item><item><term>NP antigen</term></item><item><term>Rapid diagnosis</term></item><item><term>Immunocapture ELISA</term></item><item><term>Nasal secretion</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1989">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-7QBPG8ZB-5/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VIRMET</jid><aid>8990102X</aid><ce:pii>0166-0934(89)90102-X</ce:pii><ce:doi>10.1016/0166-0934(89)90102-X</ce:doi><ce:copyright type="unknown" year="1989"></ce:copyright></item-info><head><ce:title>Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test</ce:title><ce:author-group><ce:author><ce:given-name>J.J.</ce:given-name><ce:surname>Chomel</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>D.</ce:given-name><ce:surname>Thouvenot</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Onno</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C.</ce:given-name><ce:surname>Kaiser</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.M.</ce:given-name><ce:surname>Gourreau</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Aymard</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>1</ce:label><ce:textfn>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>2</ce:label><ce:textfn>Station de Pathologie Porcine, Ploufragan, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>3</ce:label><ce:textfn>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: Dr. J.J. Chomel, Laboratoire de Virologie, Faculté de Médecine, 8, avenue Rockefeller, 69373 Lyon Cedex, France.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="24" month="2" year="1989"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>An immunocapture ELISA test for the diagnosis of human and animal influenza A and/or B is described. A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. It also permitted diagnosis in frozen samples (− 20°C) or in infected LLCMK2 cells mixed with uninfected nasopharyngeal cells and kept at 20°C for one week.</ce:simple-para><ce:simple-para>This test can be carried out in 3 h.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Influenza</ce:text></ce:keyword><ce:keyword><ce:text>NP antigen</ce:text></ce:keyword><ce:keyword><ce:text>Rapid diagnosis</ce:text></ce:keyword><ce:keyword><ce:text>Immunocapture ELISA</ce:text></ce:keyword><ce:keyword><ce:text>Nasal secretion</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test</title></titleInfo><name type="personal"><namePart type="given">J.J.</namePart><namePart type="family">Chomel</namePart><affiliation>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</affiliation><description>Correspondence to: Dr. J.J. Chomel, Laboratoire de Virologie, Faculté de Médecine, 8, avenue Rockefeller, 69373 Lyon Cedex, France.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Thouvenot</namePart><affiliation>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Onno</namePart><affiliation>Station de Pathologie Porcine, Ploufragan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Kaiser</namePart><affiliation>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.M.</namePart><namePart type="family">Gourreau</namePart><affiliation>Laboratoire de Recherche Vétérinaire, Maisons Alfort, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Aymard</namePart><affiliation>Laboratoire de Virologie. Faculté de Médecine, Lyon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: An immunocapture ELISA test for the diagnosis of human and animal influenza A and/or B is described. A monoclonal anti-nucleoprotein (NP) antibody was used to capture the NP antigen and the captured antigen was detected by an anti-NP polyclonal rabbit antiserum. Compared with the usual diagnostic method by cultivation in embryonated eggs, this test had a high specificity (97%) and sensitivity when used for diagnosis using clinical nasopharyngeal samples obtained from patients and animals. Immunocapture ELISA permitted an easier reading than the indirect immunofluorescence technique. It also permitted diagnosis in frozen samples (− 20°C) or in infected LLCMK2 cells mixed with uninfected nasopharyngeal cells and kept at 20°C for one week.This test can be carried out in 3 h.</abstract><subject><genre>Keywords</genre><topic>Influenza</topic><topic>NP antigen</topic><topic>Rapid diagnosis</topic><topic>Immunocapture ELISA</topic><topic>Nasal secretion</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Virological Methods</title></titleInfo><titleInfo type="abbreviated"><title>VIRMET</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued></originInfo><identifier type="ISSN">0166-0934</identifier><identifier type="PII">S0166-0934(00)X0143-7</identifier><part><date>1989</date><detail type="volume"><number>25</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>122</end></extent><extent unit="pages"><start>81</start><end>91</end></extent></part></relatedItem><identifier type="istex">530BE6557D7CEA2C14275DEBC1B1569F1801A53D</identifier><identifier type="ark">ark:/67375/6H6-7QBPG8ZB-5</identifier><identifier type="DOI">10.1016/0166-0934(89)90102-X</identifier><identifier type="PII">0166-0934(89)90102-X</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-7QBPG8ZB-5/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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