Development of a mammalian cell (Vero) derived candidate influenza virus vaccine
Identifieur interne : 000F30 ( Istex/Corpus ); précédent : 000F29; suivant : 000F31Development of a mammalian cell (Vero) derived candidate influenza virus vaccine
Auteurs : O. Kistner ; P. N. Barrett ; W. Mundt ; M. Reiter ; S. Schober-Bendixen ; F. DornerSource :
- Vaccine [ 0264-410X ] ; 1998.
English descriptors
- Teeft :
- Adventitious viruses, Allantoic fluid, Assay, Biol, Blood cells, Cell bank, Cell culture, Cell culture medium, Cell line, Cell lines, Cell number, Chemical accurates, Chorioallantoic membrane, Continuous cell lines, Corresponding strains, Corresponding vero, Culture medium, Direct comparison, Effective dose, Elisa, Elsevier science, Embryonated, Embryonated chicken eggs, Embryonated eggs, Fermenter, Formalin, Gentle agitation, Hemagglutination, Hemagglutinin, Hlnl, Horseradish peroxidase, Host system, Human vaccines, Immunization, Industrial production, Infectious virus, Influenza, Influenza strains, Influenza subunit vaccine, Influenza vaccine, Influenza vaccine production, Influenza vaccines, Influenza virus, Influenza virus preparations, Influenza virus vaccine strains, Influenza viruses, Johannesburg, Kistner, Large quantities, Larger volume, Master cell bank, Maximum virus, Mdck cells, Microtitre plate, Monolayer cultures, More immunogenic, Mouse sera, Nanchang, Neuraminidase, Neuraminidase activity, Nibsc, Optical density, Passage level, Passaged, Peak fractions, Precipitation zones, Protamine sulphate precipitation, Purification procedure, Purification scheme, Purification steps, Rapid production, Reagent, Roller bottles, Room temperature, Specific sera, Standard dose, Sucrose gradient, Tissue culture, Titre, Transcriptase assay, Trypsin, Vaccine, Vaccine production, Vaccine strains, Vcro virus, Vero, Vero cell, Vero cell culture, Vero cell cultures, Vero cell line, Vero cell system, Vero cells, Vero nanchang, Vero vaccine, Viral, Viral contamination, Virology, Virus, Virus antigen, Virus preparations, Virus strains, Virus vaccine, Western blot analysis, Westernblot analysis, Wide range.
Abstract
Abstract: Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation in a mouse model.
Url:
DOI: 10.1016/S0264-410X(97)00301-0
Links to Exploration step
ISTEX:1AE5DDAE72479A46D53ACAF0C702D8A3B7A6AB2FLe document en format XML
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Mundt</name><affiliation><mods:affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</mods:affiliation></affiliation></author><author><name sortKey="Reiter, M" sort="Reiter, M" uniqKey="Reiter M" first="M." last="Reiter">M. Reiter</name><affiliation><mods:affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</mods:affiliation></affiliation></author><author><name sortKey="Schober Bendixen, S" sort="Schober Bendixen, S" uniqKey="Schober Bendixen S" first="S." last="Schober-Bendixen">S. Schober-Bendixen</name><affiliation><mods:affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</mods:affiliation></affiliation></author><author><name sortKey="Dorner, F" sort="Dorner, F" uniqKey="Dorner F" first="F." last="Dorner">F. 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Kistner</name><affiliation><mods:affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</mods:affiliation></affiliation></author><author><name sortKey="Barrett, P N" sort="Barrett, P N" uniqKey="Barrett P" first="P. N." last="Barrett">P. N. Barrett</name><affiliation><mods:affiliation>Author to whom all correspondence should be addressed.</mods:affiliation></affiliation><affiliation><mods:affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</mods:affiliation></affiliation></author><author><name sortKey="Mundt, W" sort="Mundt, W" uniqKey="Mundt W" first="W." last="Mundt">W. Mundt</name><affiliation><mods:affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</mods:affiliation></affiliation></author><author><name sortKey="Reiter, M" sort="Reiter, M" uniqKey="Reiter M" first="M." last="Reiter">M. 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This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. 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Barrett</name><affiliations><json:string>Author to whom all correspondence should be addressed.</json:string><json:string>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</json:string></affiliations></json:item><json:item><name>W. Mundt</name><affiliations><json:string>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</json:string></affiliations></json:item><json:item><name>M. Reiter</name><affiliations><json:string>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</json:string></affiliations></json:item><json:item><name>S. Schober-Bendixen</name><affiliations><json:string>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</json:string></affiliations></json:item><json:item><name>F. Dorner</name><affiliations><json:string>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Vero cell</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>influenza vaccine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>serum free</value></json:item></subject><arkIstex>ark:/67375/6H6-DJ5TG46Q-G</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation in a mouse model.</abstract><qualityIndicators><score>8.5</score><pdfWordCount>5608</pdfWordCount><pdfCharCount>40708</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>614 x 857 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>125</abstractWordCount><abstractCharCount>813</abstractCharCount><keywordCount>3</keywordCount></qualityIndicators><title>Development of a mammalian cell (Vero) derived candidate influenza virus vaccine</title><pmid><json:string>9682344</json:string></pmid><pii><json:string>S0264-410X(97)00301-0</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Options for the Control of Influenza III. Proceedings of the Third International Conference on Options for the Control of Influenza</title><language><json:string>unknown</json:string></language><pages><first>683</first><last>693</last></pages><editor><json:item><name>L.E. Brown</name></json:item><json:item><name>A.W. Hampson</name></json:item><json:item><name>R.G. Webster</name></json:item></editor><conference><json:item><name>Options for the Control of Influenza III. Proceedings of the Third International Conference on Options for the Control of Influenza - Cairns, Australia, 4–9 May 1996</name></json:item></conference></serie><host><title>Vaccine</title><language><json:string>unknown</json:string></language><publicationDate>1998</publicationDate><issn><json:string>0264-410X</json:string></issn><pii><json:string>S0264-410X(00)X0059-X</json:string></pii><volume>16</volume><issue>9–10</issue><pages><first>960</first><last>968</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1998</json:string><json:string>1995</json:string><json:string>1962</json:string><json:string>1200</json:string><json:string>1996</json:string><json:string>2560</json:string></date><geogName></geogName><orgName><json:string>WHO</json:string><json:string>Virotech</json:string><json:string>Biomedical Research Center, Baxter</json:string><json:string>UK and Austria</json:string><json:string>American Type Tissue Collection</json:string><json:string>Elsevier Science Ltd.</json:string><json:string>ATCC</json:string><json:string>World Health Organisation</json:string><json:string>National Institute for Biological Standards and Control</json:string><json:string>Dynatech</json:string><json:string>Institute of Virology</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>M. 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Molecular Medicine</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1016/S0264-410X(97)00301-0</json:string></doi><id>1AE5DDAE72479A46D53ACAF0C702D8A3B7A6AB2F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DJ5TG46Q-G/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DJ5TG46Q-G/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-DJ5TG46Q-G/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Development of a mammalian cell (Vero) derived candidate influenza virus vaccine</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1998</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Paper</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Development of a mammalian cell (Vero) derived candidate influenza virus vaccine</title><author xml:id="author-0000"><persName><forename type="first">O</forename><surname>Kistner</surname></persName><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation></author><author xml:id="author-0001"><persName><forename type="first">P.N.</forename><surname>Barrett</surname></persName><affiliation>Author to whom all correspondence should be addressed.</affiliation><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation></author><author xml:id="author-0002"><persName><forename type="first">W.</forename><surname>Mundt</surname></persName><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation></author><author xml:id="author-0003"><persName><forename type="first">M.</forename><surname>Reiter</surname></persName><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation></author><author xml:id="author-0004"><persName><forename type="first">S.</forename><surname>Schober-Bendixen</surname></persName><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation></author><author xml:id="author-0005"><persName><forename type="first">F.</forename><surname>Dorner</surname></persName><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation></author><idno type="istex">1AE5DDAE72479A46D53ACAF0C702D8A3B7A6AB2F</idno><idno type="ark">ark:/67375/6H6-DJ5TG46Q-G</idno><idno type="DOI">10.1016/S0264-410X(97)00301-0</idno><idno type="PII">S0264-410X(97)00301-0</idno></analytic><monogr><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="pISSN">0264-410X</idno><idno type="PII">S0264-410X(00)X0059-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">16</biblScope><biblScope unit="issue">9–10</biblScope><biblScope unit="page" from="960">960</biblScope><biblScope unit="page" to="968">968</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation in a mouse model.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Vero cell</term></item><item><term>influenza vaccine</term></item><item><term>serum free</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1997-10-24">Modified</change><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DJ5TG46Q-G/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JVAC</jid><aid>97003010</aid><ce:pii>S0264-410X(97)00301-0</ce:pii><ce:doi>10.1016/S0264-410X(97)00301-0</ce:doi><ce:copyright type="unknown" year="1998"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Paper</ce:textfn></ce:dochead><ce:title>Development of a mammalian cell (Vero) derived candidate influenza virus vaccine</ce:title><ce:author-group><ce:author><ce:given-name>O</ce:given-name><ce:surname>Kistner</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>P.N.</ce:given-name><ce:surname>Barrett</ce:surname><ce:cross-ref refid="COR1"><ce:sup>†</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>W.</ce:given-name><ce:surname>Mundt</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Reiter</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>Schober-Bendixen</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>F.</ce:given-name><ce:surname>Dorner</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>∗</ce:label><ce:textfn>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>†</ce:label><ce:text>Author to whom all correspondence should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="15" month="7" year="1997"></ce:date-received><ce:date-revised day="24" month="10" year="1997"></ce:date-revised><ce:date-accepted day="28" month="10" year="1997"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation in a mouse model.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Vero cell</ce:text></ce:keyword><ce:keyword><ce:text>influenza vaccine</ce:text></ce:keyword><ce:keyword><ce:text>serum free</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Development of a mammalian cell (Vero) derived candidate influenza virus vaccine</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Development of a mammalian cell (Vero) derived candidate influenza virus vaccine</title></titleInfo><name type="personal"><namePart type="given">O</namePart><namePart type="family">Kistner</namePart><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.N.</namePart><namePart type="family">Barrett</namePart><affiliation>Author to whom all correspondence should be addressed.</affiliation><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Mundt</namePart><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Reiter</namePart><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Schober-Bendixen</namePart><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Dorner</namePart><affiliation>Biomedical Research Center, Baxter-Immuno, Uferstr. 15, A-2304 Orth/Donau, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><dateModified encoding="w3cdtf">1997-10-24</dateModified><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The World Health Organisation (WHO) approved Vero cell line was used in serum-free culture to grow a multitude of influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter volume. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation in a mouse model.</abstract><note type="content">Section title: Paper</note><subject><genre>Keywords</genre><topic>Vero cell</topic><topic>influenza vaccine</topic><topic>serum free</topic></subject><relatedItem type="host"><titleInfo><title>Vaccine</title></titleInfo><titleInfo type="abbreviated"><title>JVAC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued></originInfo><identifier type="ISSN">0264-410X</identifier><identifier type="PII">S0264-410X(00)X0059-X</identifier><part><date>1998</date><detail type="volume"><number>16</number><caption>vol.</caption></detail><detail type="issue"><number>9–10</number><caption>no.</caption></detail><extent unit="issue-pages"><start>871</start><end>1064</end></extent><extent unit="pages"><start>960</start><end>968</end></extent></part></relatedItem><identifier type="istex">1AE5DDAE72479A46D53ACAF0C702D8A3B7A6AB2F</identifier><identifier type="ark">ark:/67375/6H6-DJ5TG46Q-G</identifier><identifier type="DOI">10.1016/S0264-410X(97)00301-0</identifier><identifier type="PII">S0264-410X(97)00301-0</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-DJ5TG46Q-G/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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