Phenotypic expression of HA-NA combinations in human-avian influenza A virus reassortants
Identifieur interne : 000E70 ( Istex/Corpus ); précédent : 000E69; suivant : 000E71Phenotypic expression of HA-NA combinations in human-avian influenza A virus reassortants
Auteurs : I. A. Rudneva ; E. I. Sklyanskaya ; O. S. Barulina ; S. S. Yamnikova ; V. P. Kovaleva ; I. V. Tsvetkova ; N. V. KaverinSource :
- Archives of Virology [ 0304-8608 ] ; 1996-06-01.
English descriptors
- Teeft :
- Actual virion yields, Aggregation, Allantoic, Allantoic fluid, Chick embryos, Embryonated chicken eggs, Enzymatic activity, Hemagglutinin, Influenza, Influenza virus, Initial reassortants, Monoclonal antibodies, Natural conditions, Neuraminidase, Other genes, Parent virus, Parent viruses, Passage variants, Passaged, Passaged variant, Passaged variants, Previous communication, Reassortant, Reassortants, Rudneva, Rwb1, Serial passages, Subtypes, Such reassortants, Sucrose, Sucrose cushion, Titer, Variant, Virion, Virion aggregates, Virion aggregation, Virus, Virus reassortants, Virus yields.
Abstract
Summary: Human-avian and human-mammalian influenza A virus reassortant clones with the neuraminidase (NA) gene of the A/USSR/90/77 (H1N1) strain and hemagglutinin (HA) genes of H3, H4 and H13 subtypes had been shown in an earlier publication to produce low HA yields in the embryonated chicken eggs. The low HA titers had been shown to be due, at least in part, to the formation of virion clusters at 4°C; the clustering was removed by the treatment with bacterial neuraminidase [Rudneva et al., Arch. Virol (1993) 133: 437–450]. By serial passages of the reassortants in chick embryos non-aggregating variants were selected: the variants produced HA titers of the same order as A/USSR/90/77 parent virus. The assessment of the virus yields by the analysis of the partially purified virus preparations from fixed volumes of the allantoic fluid revealed that actual virion yields of the initial reassortants were lower than the yields of their passaged variants or of the parent viruses. The passaged variant of a reassortant possessing the HA gene of A/Duck/Ukraine/1/63 (H3N2) virus differed from the original (non-passaged) reassortant and from the parent A/Duck/Ukraine/1/63 virus in the reaction with a panel of monoclonal antibodies against H3 hemagglutinin. The data suggest that some HA-NA combinations may lead to an incomplete functional match between HA and NA and to the formation of low-yield reassortants, thus representing a possible limiting factor in the emergence of new HA-NA combinations in natural conditions.
Url:
DOI: 10.1007/BF01718612
Links to Exploration step
ISTEX:9741B3D32289A1CF551D931DBA38DD64F02F0F3FLe document en format XML
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The low HA titers had been shown to be due, at least in part, to the formation of virion clusters at 4°C; the clustering was removed by the treatment with bacterial neuraminidase [Rudneva et al., Arch. Virol (1993) 133: 437–450]. By serial passages of the reassortants in chick embryos non-aggregating variants were selected: the variants produced HA titers of the same order as A/USSR/90/77 parent virus. The assessment of the virus yields by the analysis of the partially purified virus preparations from fixed volumes of the allantoic fluid revealed that actual virion yields of the initial reassortants were lower than the yields of their passaged variants or of the parent viruses. The passaged variant of a reassortant possessing the HA gene of A/Duck/Ukraine/1/63 (H3N2) virus differed from the original (non-passaged) reassortant and from the parent A/Duck/Ukraine/1/63 virus in the reaction with a panel of monoclonal antibodies against H3 hemagglutinin. 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Diseases</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>141</VolumeIDStart><VolumeIDEnd>141</VolumeIDEnd><VolumeIssueCount>12</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>6</IssueIDStart><IssueIDEnd>6</IssueIDEnd><IssueArticleCount>16</IssueArticleCount><IssueHistory><CoverDate><DateString>1996</DateString><Year>1996</Year><Month>6</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1996</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art10"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF01718612</ArticleID><ArticleDOI>10.1007/BF01718612</ArticleDOI><ArticleSequenceNumber>10</ArticleSequenceNumber><ArticleTitle Language="En">Phenotypic expression of HA-NA combinations in human-avian influenza A virus reassortants</ArticleTitle><ArticleCategory>Original Papers</ArticleCategory><ArticleFirstPage>1091</ArticleFirstPage><ArticleLastPage>1099</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>5</Month><Day>3</Day></RegistrationDate><Received><Year>1995</Year><Month>7</Month><Day>15</Day></Received><Accepted><Year>1996</Year><Month>1</Month><Day>19</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1996</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>705</JournalID><VolumeIDStart>141</VolumeIDStart><VolumeIDEnd>141</VolumeIDEnd><IssueIDStart>6</IssueIDStart><IssueIDEnd>6</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>I.</GivenName><GivenName>A.</GivenName><FamilyName>Rudneva</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>E.</GivenName><GivenName>I.</GivenName><FamilyName>Sklyanskaya</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>O.</GivenName><GivenName>S.</GivenName><FamilyName>Barulina</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>S.</GivenName><GivenName>S.</GivenName><FamilyName>Yamnikova</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>V.</GivenName><GivenName>P.</GivenName><FamilyName>Kovaleva</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>I.</GivenName><GivenName>V.</GivenName><FamilyName>Tsvetkova</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>N.</GivenName><GivenName>V.</GivenName><FamilyName>Kaverin</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgName>The D. I. Ivanovsky Institute of Virology</OrgName><OrgAddress><City>Moscow</City><Country>Russia</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>Institute of Biological and Medical Chemistry</OrgName><OrgAddress><City>Moscow</City><Country>Russia</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>Human-avian and human-mammalian influenza A virus reassortant clones with the neuraminidase (NA) gene of the A/USSR/90/77 (H1N1) strain and hemagglutinin (HA) genes of H3, H4 and H13 subtypes had been shown in an earlier publication to produce low HA yields in the embryonated chicken eggs. The low HA titers had been shown to be due, at least in part, to the formation of virion clusters at 4°C; the clustering was removed by the treatment with bacterial neuraminidase [Rudneva et al., Arch. Virol (1993) 133: 437–450]. By serial passages of the reassortants in chick embryos non-aggregating variants were selected: the variants produced HA titers of the same order as A/USSR/90/77 parent virus. The assessment of the virus yields by the analysis of the partially purified virus preparations from fixed volumes of the allantoic fluid revealed that actual virion yields of the initial reassortants were lower than the yields of their passaged variants or of the parent viruses. The passaged variant of a reassortant possessing the HA gene of A/Duck/Ukraine/1/63 (H3N2) virus differed from the original (non-passaged) reassortant and from the parent A/Duck/Ukraine/1/63 virus in the reaction with a panel of monoclonal antibodies against H3 hemagglutinin. The data suggest that some HA-NA combinations may lead to an incomplete functional match between HA and NA and to the formation of low-yield reassortants, thus representing a possible limiting factor in the emergence of new HA-NA combinations in natural conditions.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Phenotypic expression of HA-NA combinations in human-avian influenza A virus reassortants</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Phenotypic expression of HA-NA combinations in human-avian influenza A virus reassortants</title></titleInfo><name type="personal"><namePart type="given">I.</namePart><namePart type="given">A.</namePart><namePart type="family">Rudneva</namePart><affiliation>The D. I. Ivanovsky Institute of Virology, Moscow, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="given">I.</namePart><namePart type="family">Sklyanskaya</namePart><affiliation>The D. I. Ivanovsky Institute of Virology, Moscow, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="given">S.</namePart><namePart type="family">Barulina</namePart><affiliation>The D. I. Ivanovsky Institute of Virology, Moscow, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="given">S.</namePart><namePart type="family">Yamnikova</namePart><affiliation>The D. I. Ivanovsky Institute of Virology, Moscow, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="given">P.</namePart><namePart type="family">Kovaleva</namePart><affiliation>The D. I. Ivanovsky Institute of Virology, Moscow, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="given">V.</namePart><namePart type="family">Tsvetkova</namePart><affiliation>Institute of Biological and Medical Chemistry, Moscow, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.</namePart><namePart type="given">V.</namePart><namePart type="family">Kaverin</namePart><affiliation>The D. I. Ivanovsky Institute of Virology, Moscow, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">1995-07-15</dateCreated><dateIssued encoding="w3cdtf">1996-06-01</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Summary: Human-avian and human-mammalian influenza A virus reassortant clones with the neuraminidase (NA) gene of the A/USSR/90/77 (H1N1) strain and hemagglutinin (HA) genes of H3, H4 and H13 subtypes had been shown in an earlier publication to produce low HA yields in the embryonated chicken eggs. The low HA titers had been shown to be due, at least in part, to the formation of virion clusters at 4°C; the clustering was removed by the treatment with bacterial neuraminidase [Rudneva et al., Arch. Virol (1993) 133: 437–450]. By serial passages of the reassortants in chick embryos non-aggregating variants were selected: the variants produced HA titers of the same order as A/USSR/90/77 parent virus. The assessment of the virus yields by the analysis of the partially purified virus preparations from fixed volumes of the allantoic fluid revealed that actual virion yields of the initial reassortants were lower than the yields of their passaged variants or of the parent viruses. The passaged variant of a reassortant possessing the HA gene of A/Duck/Ukraine/1/63 (H3N2) virus differed from the original (non-passaged) reassortant and from the parent A/Duck/Ukraine/1/63 virus in the reaction with a panel of monoclonal antibodies against H3 hemagglutinin. The data suggest that some HA-NA combinations may lead to an incomplete functional match between HA and NA and to the formation of low-yield reassortants, thus representing a possible limiting factor in the emergence of new HA-NA combinations in natural conditions.</abstract><note>Original Papers</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title></titleInfo><titleInfo type="abbreviated"><title>Archives of Virology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1996-06-01</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Virology</topic><topic>Infectious Diseases</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">16</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>1996</date><detail type="volume"><number>141</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>1091</start><end>1099</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1996</recordOrigin></recordInfo></relatedItem><identifier type="istex">9741B3D32289A1CF551D931DBA38DD64F02F0F3F</identifier><identifier type="ark">ark:/67375/1BB-RTG20C0G-P</identifier><identifier type="DOI">10.1007/BF01718612</identifier><identifier type="ArticleID">BF01718612</identifier><identifier type="ArticleID">Art10</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1996</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1996</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-RTG20C0G-P/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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