Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones
Identifieur interne : 000C11 ( Istex/Corpus ); précédent : 000C10; suivant : 000C12Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones
Auteurs : Jacqueline M. Katz ; Lorena E. Brown ; Rosemary A. Ffrench ; David O. WhiteSource :
- Vaccine [ 0264-410X ] ; 1985.
English descriptors
- Teeft :
- Antibody response, Assay, Cell clone, Cell clones, Cell determinants, Cell line, Cell lines, Clone, Culture medium, Determinant, Different subtypes, Different type, Haemagglutinin, Helper, Immunol, Influenza, Influenza virus, Influenza virus haemagglutinin, Influenza viruses, Katz, Monoclonal antibodies, Proliferation assays, Proliferative, Proliferative response, Same subtype, Spleen, Spleen cells, Subtypes, Various doses, Virus.
Abstract
Abstract: Helper T cell lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.
Url:
DOI: 10.1016/0264-410X(85)90118-5
Links to Exploration step
ISTEX:E418096C719D6591FB4BB3CB69B19BD4FE3978CALe document en format XML
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Katz</name><affiliation><mods:affiliation>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</mods:affiliation></affiliation></author><author><name sortKey="Brown, Lorena E" sort="Brown, Lorena E" uniqKey="Brown L" first="Lorena E." last="Brown">Lorena E. Brown</name><affiliation><mods:affiliation>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</mods:affiliation></affiliation></author><author><name sortKey="Ffrench, Rosemary A" sort="Ffrench, Rosemary A" uniqKey="Ffrench R" first="Rosemary A." last="Ffrench">Rosemary A. Ffrench</name><affiliation><mods:affiliation>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</mods:affiliation></affiliation></author><author><name sortKey="White, David O" sort="White, David O" uniqKey="White D" first="David O." last="White">David O. 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At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. 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Katz</name><affiliations><json:string>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</json:string></affiliations></json:item><json:item><name>Lorena E. Brown</name><affiliations><json:string>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</json:string></affiliations></json:item><json:item><name>Rosemary A. Ffrench</name><affiliations><json:string>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</json:string></affiliations></json:item><json:item><name>David O. 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At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.</abstract><qualityIndicators><score>8.71</score><pdfWordCount>4190</pdfWordCount><pdfCharCount>24475</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>6</pdfPageCount><pdfPageSize>620 x 864 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>210</abstractWordCount><abstractCharCount>1315</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones</title><pmid><json:string>2414940</json:string></pmid><pii><json:string>0264-410X(85)90118-5</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>II. Specificity of helper T cells recognizing haemagglutinin</title><language><json:string>unknown</json:string></language><pages><first>669</first></pages></serie><host><title>Vaccine</title><language><json:string>unknown</json:string></language><publicationDate>1985</publicationDate><issn><json:string>0264-410X</json:string></issn><pii><json:string>S0264-410X(00)X0159-4</json:string></pii><volume>3</volume><issue>3</issue><pages><first>257</first><last>262</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1985</json:string></date><geogName></geogName><orgName><json:string>Division of Protein Chemistry</json:string><json:string>Postdoctoral Research Fellowship</json:string><json:string>Medical Research, ANU</json:string><json:string>Flow Laboratories</json:string><json:string>Medical Research Council of Australia</json:string><json:string>S</json:string><json:string>National Vaccine, Vol. 3</json:string></orgName><orgName_funder><json:string>S</json:string><json:string>National Vaccine, Vol. 3</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Clones</json:string><json:string>C. 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At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Viruses</term></item><item><term>influenza</term></item><item><term>T lymphocytes</term></item><item><term>haemagglutinin</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1985">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-2DCKL1FW-M/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JVAC</jid><aid>85901185</aid><ce:pii>0264-410X(85)90118-5</ce:pii><ce:doi>10.1016/0264-410X(85)90118-5</ce:doi><ce:copyright type="unknown" year="1985"></ce:copyright></item-info><head><ce:title>Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones</ce:title><ce:author-group><ce:author><ce:given-name>Jacqueline M.</ce:given-name><ce:surname>Katz</ce:surname></ce:author><ce:author><ce:given-name>Lorena E.</ce:given-name><ce:surname>Brown</ce:surname></ce:author><ce:author><ce:given-name>Rosemary A.</ce:given-name><ce:surname>Ffrench</ce:surname></ce:author><ce:author><ce:given-name>David O.</ce:given-name><ce:surname>White</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Helper T cell lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. At line stimulated <ce:italic>in vitro</ce:italic> with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated <ce:italic>in vitro</ce:italic> with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA<ce:inf>1</ce:inf> chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells <ce:italic>in vitro</ce:italic>. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Viruses</ce:text></ce:keyword><ce:keyword><ce:text>influenza</ce:text></ce:keyword><ce:keyword><ce:text>T lymphocytes</ce:text></ce:keyword><ce:keyword><ce:text>haemagglutinin</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Murine helper T lymphocyte response to influenza virus: recognition of haemagglutinin by subtype-specific and cross-reactive T cell clones</title></titleInfo><name type="personal"><namePart type="given">Jacqueline M.</namePart><namePart type="family">Katz</namePart><affiliation>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lorena E.</namePart><namePart type="family">Brown</namePart><affiliation>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rosemary A.</namePart><namePart type="family">Ffrench</namePart><affiliation>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David O.</namePart><namePart type="family">White</namePart><affiliation>Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued><copyrightDate encoding="w3cdtf">1985</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Helper T cell lines specific for influenza virus were established by continuous culture of Mem 71-Bel (H3) virus-immune spleen cells in the presence of virus and antigen-presenting cells and their specificity assessed in proliferation experiments. At line stimulated in vitro with Mem 71-Bel virus was able to proliferate in response to viruses of the same, and also of different, type A haemagglutinin (HA) subtypes as the immunizing virus but not to a type B influenza virus. A component of this cross-reactivity was due to recognition of the HA molecule. Lines stimulated in vitro with purified disrupted H3 or H2 viruses showed a higher degree of cross-reactivity. Of nine clones isolated from these lines, seven were directed against the HA molecule and recognized the HA1 chain. The HA-specific T cell clones were either subtype-specific, recognizing only viruses of the H3 subtype, or cross-reactive, also recognizing viruses of the H2 subtype of type A (but not type B). Subtype-specific and cross-reactive T cell clones were shown to function as helper T cells in vitro. In addition to collaborating with H3 virus-primed B cells responding to H3 virus in culture, the cross-reactive T cell clone could also provide help for H2 virus-primed B cells making anti-HA antibody in response to H2 virus.</abstract><subject><genre>Keywords</genre><topic>Viruses</topic><topic>influenza</topic><topic>T lymphocytes</topic><topic>haemagglutinin</topic></subject><relatedItem type="host"><titleInfo><title>Vaccine</title></titleInfo><titleInfo type="abbreviated"><title>JVAC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued></originInfo><identifier type="ISSN">0264-410X</identifier><identifier type="PII">S0264-410X(00)X0159-4</identifier><part><date>1985</date><detail type="volume"><number>3</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>161</start><end>280</end></extent><extent unit="pages"><start>257</start><end>262</end></extent></part></relatedItem><identifier type="istex">E418096C719D6591FB4BB3CB69B19BD4FE3978CA</identifier><identifier type="ark">ark:/67375/6H6-2DCKL1FW-M</identifier><identifier type="DOI">10.1016/0264-410X(85)90118-5</identifier><identifier type="PII">0264-410X(85)90118-5</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-2DCKL1FW-M/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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