Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure
Identifieur interne : 000B08 ( Istex/Corpus ); précédent : 000B07; suivant : 000B09Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure
Auteurs : Jürgen Werner ; Olaf Thraenhart ; Ernst KuwertSource :
- Medical Microbiology and Immunology [ 0300-8584 ] ; 1978-11-01.
English descriptors
- Teeft :
- Antigenic, Antigenic drift, Antigenic relationship, Antigenic relationships, Antigenic variation, Antigenic variations, Assay, Biol, Enzyme activity, Fetuin, Hongkong, Hongkong virus, Influenza, Influenza virus, Influenza virus neuraminidase, Influenza viruses, Kuwert, Major variations, Nana, National influenza center, Neuraminidase, Neuraminidase antigens, Niso titers, Port chalmers, Recombinant, Recombinant strains, Representative influenza, Sialic acids, Specific antiserums, Standard procedure, Surface antigens, Test procedures, Thiobarbituric acid assay, Viral neuraminidase.
Abstract
Abstract: A new rapid modified micro-neuraminidase-inhibition test (ESSEN-NIT) has recently been described. This test was originally devised to facilitate largescale screening for serum antibodies to influenza virus neuraminidase. It was shown that this test yielded results comparable to those obtained with the WHO standard procedure. Our report presents data on the comparison between the WHO method and the ESSEN-NIT with respect to their capability in detecting antigenic differences in neuraminidase of various strains of influenza A viruses belonging to the H3N2 subtype family. Two antiserums against the N2 antigens of the A/Hongkong/1/68 (X15HK) and of the A/Port Chalmers/1/73 (X42) recombinant strains were used in characterization experiments. The results obtained indicate that the ESSEN-NIT is at least as sensitive in detecting antigenic variations of neuraminidase as the WHO standard procedure. The drift of the N2 enzyme which occurred as early as 1969 and is known to continue to date, could be clearly demonstrated. Major and minor antigenic changes of neuraminidase in representative influenza isolates were equally well detected by both assays. The ESSEN-NIT offers the advantage of speed, economy, and computerized evalutaion of data.
Url:
DOI: 10.1007/BF02121146
Links to Exploration step
ISTEX:34F0F6AB9F0A0EB1B5B5A7D8B5F301EFBDBA519ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</title><author><name sortKey="Werner, Jurgen" sort="Werner, Jurgen" uniqKey="Werner J" first="Jürgen" last="Werner">Jürgen Werner</name><affiliation><mods:affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</mods:affiliation></affiliation></author><author><name sortKey="Thraenhart, Olaf" sort="Thraenhart, Olaf" uniqKey="Thraenhart O" first="Olaf" last="Thraenhart">Olaf Thraenhart</name><affiliation><mods:affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</mods:affiliation></affiliation></author><author><name sortKey="Kuwert, Ernst" sort="Kuwert, Ernst" uniqKey="Kuwert E" first="Ernst" last="Kuwert">Ernst Kuwert</name><affiliation><mods:affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:34F0F6AB9F0A0EB1B5B5A7D8B5F301EFBDBA519A</idno><date when="1978" year="1978">1978</date><idno type="doi">10.1007/BF02121146</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-BS9C2SWL-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000B08</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B08</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</title><author><name sortKey="Werner, Jurgen" sort="Werner, Jurgen" uniqKey="Werner J" first="Jürgen" last="Werner">Jürgen Werner</name><affiliation><mods:affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</mods:affiliation></affiliation></author><author><name sortKey="Thraenhart, Olaf" sort="Thraenhart, Olaf" uniqKey="Thraenhart O" first="Olaf" last="Thraenhart">Olaf Thraenhart</name><affiliation><mods:affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</mods:affiliation></affiliation></author><author><name sortKey="Kuwert, Ernst" sort="Kuwert, Ernst" uniqKey="Kuwert E" first="Ernst" last="Kuwert">Ernst Kuwert</name><affiliation><mods:affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Medical Microbiology and Immunology</title><title level="j" type="abbrev">Med Microbiol Immunol</title><idno type="ISSN">0300-8584</idno><idno type="eISSN">1432-1831</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1978-11-01">1978-11-01</date><biblScope unit="volume">166</biblScope><biblScope unit="issue">1-4</biblScope><biblScope unit="page" from="165">165</biblScope><biblScope unit="page" to="171">171</biblScope></imprint><idno type="ISSN">0300-8584</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0300-8584</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antigenic</term><term>Antigenic drift</term><term>Antigenic relationship</term><term>Antigenic relationships</term><term>Antigenic variation</term><term>Antigenic variations</term><term>Assay</term><term>Biol</term><term>Enzyme activity</term><term>Fetuin</term><term>Hongkong</term><term>Hongkong virus</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus neuraminidase</term><term>Influenza viruses</term><term>Kuwert</term><term>Major variations</term><term>Nana</term><term>National influenza center</term><term>Neuraminidase</term><term>Neuraminidase antigens</term><term>Niso titers</term><term>Port chalmers</term><term>Recombinant</term><term>Recombinant strains</term><term>Representative influenza</term><term>Sialic acids</term><term>Specific antiserums</term><term>Standard procedure</term><term>Surface antigens</term><term>Test procedures</term><term>Thiobarbituric acid assay</term><term>Viral neuraminidase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A new rapid modified micro-neuraminidase-inhibition test (ESSEN-NIT) has recently been described. This test was originally devised to facilitate largescale screening for serum antibodies to influenza virus neuraminidase. It was shown that this test yielded results comparable to those obtained with the WHO standard procedure. Our report presents data on the comparison between the WHO method and the ESSEN-NIT with respect to their capability in detecting antigenic differences in neuraminidase of various strains of influenza A viruses belonging to the H3N2 subtype family. Two antiserums against the N2 antigens of the A/Hongkong/1/68 (X15HK) and of the A/Port Chalmers/1/73 (X42) recombinant strains were used in characterization experiments. The results obtained indicate that the ESSEN-NIT is at least as sensitive in detecting antigenic variations of neuraminidase as the WHO standard procedure. The drift of the N2 enzyme which occurred as early as 1969 and is known to continue to date, could be clearly demonstrated. Major and minor antigenic changes of neuraminidase in representative influenza isolates were equally well detected by both assays. The ESSEN-NIT offers the advantage of speed, economy, and computerized evalutaion of data.</div></front></TEI><istex><corpusName>springer-journals</corpusName><keywords><teeft><json:string>neuraminidase</json:string><json:string>antigenic</json:string><json:string>antigenic variation</json:string><json:string>influenza</json:string><json:string>fetuin</json:string><json:string>hongkong</json:string><json:string>influenza virus neuraminidase</json:string><json:string>influenza viruses</json:string><json:string>nana</json:string><json:string>kuwert</json:string><json:string>biol</json:string><json:string>recombinant</json:string><json:string>assay</json:string><json:string>specific antiserums</json:string><json:string>influenza virus</json:string><json:string>hongkong virus</json:string><json:string>recombinant strains</json:string><json:string>viral neuraminidase</json:string><json:string>surface antigens</json:string><json:string>representative influenza</json:string><json:string>port chalmers</json:string><json:string>national influenza center</json:string><json:string>antigenic variations</json:string><json:string>major variations</json:string><json:string>test procedures</json:string><json:string>enzyme activity</json:string><json:string>niso titers</json:string><json:string>standard procedure</json:string><json:string>antigenic drift</json:string><json:string>neuraminidase antigens</json:string><json:string>antigenic relationships</json:string><json:string>thiobarbituric acid assay</json:string><json:string>antigenic relationship</json:string><json:string>sialic acids</json:string></teeft></keywords><author><json:item><name>Jürgen Werner</name><affiliations><json:string>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</json:string></affiliations></json:item><json:item><name>Olaf Thraenhart</name><affiliations><json:string>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</json:string></affiliations></json:item><json:item><name>Ernst Kuwert</name><affiliations><json:string>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</json:string></affiliations></json:item></author><articleId><json:string>BF02121146</json:string><json:string>Art20</json:string></articleId><arkIstex>ark:/67375/1BB-BS9C2SWL-S</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: A new rapid modified micro-neuraminidase-inhibition test (ESSEN-NIT) has recently been described. This test was originally devised to facilitate largescale screening for serum antibodies to influenza virus neuraminidase. It was shown that this test yielded results comparable to those obtained with the WHO standard procedure. Our report presents data on the comparison between the WHO method and the ESSEN-NIT with respect to their capability in detecting antigenic differences in neuraminidase of various strains of influenza A viruses belonging to the H3N2 subtype family. Two antiserums against the N2 antigens of the A/Hongkong/1/68 (X15HK) and of the A/Port Chalmers/1/73 (X42) recombinant strains were used in characterization experiments. The results obtained indicate that the ESSEN-NIT is at least as sensitive in detecting antigenic variations of neuraminidase as the WHO standard procedure. The drift of the N2 enzyme which occurred as early as 1969 and is known to continue to date, could be clearly demonstrated. Major and minor antigenic changes of neuraminidase in representative influenza isolates were equally well detected by both assays. The ESSEN-NIT offers the advantage of speed, economy, and computerized evalutaion of data.</abstract><qualityIndicators><score>6.14</score><pdfWordCount>1944</pdfWordCount><pdfCharCount>12451</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>450 x 702 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>183</abstractWordCount><abstractCharCount>1258</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</title><pmid><json:string>364282</json:string></pmid><genre><json:string>research-article</json:string></genre><host><title>Medical Microbiology and Immunology</title><language><json:string>unknown</json:string></language><publicationDate>1978</publicationDate><copyrightDate>1978</copyrightDate><issn><json:string>0300-8584</json:string></issn><eissn><json:string>1432-1831</json:string></eissn><journalId><json:string>430</json:string></journalId><volume>166</volume><issue>1-4</issue><pages><first>165</first><last>171</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Immunology</value></json:item><json:item><value>Medical Microbiology</value></json:item></subject></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/1BB-BS9C2SWL-S</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - microbiology</json:string><json:string>2 - immunology</json:string></wos><scienceMetrix></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Microbiology (medical)</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Immunology and Allergy</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string><json:string>4 - psychopathologie. psychiatrie</json:string></inist></categories><publicationDate>1978</publicationDate><copyrightDate>1978</copyrightDate><doi><json:string>10.1007/BF02121146</json:string></doi><id>34F0F6AB9F0A0EB1B5B5A7D8B5F301EFBDBA519A</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-BS9C2SWL-S/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-BS9C2SWL-S/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/1BB-BS9C2SWL-S/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><availability><licence><p>Springer-Verlag, 1978</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>1978</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</title><author xml:id="author-0000" corresp="yes"><persName><forename type="first">Jürgen</forename><surname>Werner</surname></persName><affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Olaf</forename><surname>Thraenhart</surname></persName><affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Ernst</forename><surname>Kuwert</surname></persName><affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</affiliation></author><idno type="istex">34F0F6AB9F0A0EB1B5B5A7D8B5F301EFBDBA519A</idno><idno type="ark">ark:/67375/1BB-BS9C2SWL-S</idno><idno type="DOI">10.1007/BF02121146</idno><idno type="article-id">BF02121146</idno><idno type="article-id">Art20</idno></analytic><monogr><title level="j">Medical Microbiology and Immunology</title><title level="j" type="abbrev">Med Microbiol Immunol</title><idno type="pISSN">0300-8584</idno><idno type="eISSN">1432-1831</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">33</idno><idno type="volume-issue-count">4</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1978-11-01"></date><biblScope unit="volume">166</biblScope><biblScope unit="issue">1-4</biblScope><biblScope unit="page" from="165">165</biblScope><biblScope unit="page" to="171">171</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1978</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A new rapid modified micro-neuraminidase-inhibition test (ESSEN-NIT) has recently been described. This test was originally devised to facilitate largescale screening for serum antibodies to influenza virus neuraminidase. It was shown that this test yielded results comparable to those obtained with the WHO standard procedure. Our report presents data on the comparison between the WHO method and the ESSEN-NIT with respect to their capability in detecting antigenic differences in neuraminidase of various strains of influenza A viruses belonging to the H3N2 subtype family. Two antiserums against the N2 antigens of the A/Hongkong/1/68 (X15HK) and of the A/Port Chalmers/1/73 (X42) recombinant strains were used in characterization experiments. The results obtained indicate that the ESSEN-NIT is at least as sensitive in detecting antigenic variations of neuraminidase as the WHO standard procedure. The drift of the N2 enzyme which occurred as early as 1969 and is known to continue to date, could be clearly demonstrated. Major and minor antigenic changes of neuraminidase in representative influenza isolates were equally well detected by both assays. The ESSEN-NIT offers the advantage of speed, economy, and computerized evalutaion of data.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Immunology</term></item><item><term>Medical Microbiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1978-11-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-BS9C2SWL-S/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>430</JournalID><JournalPrintISSN>0300-8584</JournalPrintISSN><JournalElectronicISSN>1432-1831</JournalElectronicISSN><JournalTitle>Medical Microbiology and Immunology</JournalTitle><JournalAbbreviatedTitle>Med Microbiol Immunol</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Immunology</JournalSubject><JournalSubject Type="Secondary">Medical Microbiology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>166</VolumeIDStart><VolumeIDEnd>166</VolumeIDEnd><VolumeIssueCount>4</VolumeIssueCount></VolumeInfo><Issue IssueType="Combined"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>4</IssueIDEnd><IssueArticleCount>33</IssueArticleCount><IssueHistory><CoverDate><DateString>17. XI. 1978</DateString><Year>1978</Year><Month>11</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1978</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art20"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF02121146</ArticleID><ArticleDOI>10.1007/BF02121146</ArticleDOI><ArticleSequenceNumber>20</ArticleSequenceNumber><ArticleTitle Language="En">Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</ArticleTitle><ArticleFirstPage>165</ArticleFirstPage><ArticleLastPage>171</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>8</Month><Day>10</Day></RegistrationDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1978</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>430</JournalID><VolumeIDStart>166</VolumeIDStart><VolumeIDEnd>166</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>4</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Jürgen</GivenName><FamilyName>Werner</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Olaf</GivenName><FamilyName>Thraenhart</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Ernst</GivenName><FamilyName>Kuwert</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Institut für Medizinische Virologie und Immunologie</OrgDivision><OrgName>Universität Essen-Gesamthochschule</OrgName><OrgAddress><Street>Hufelandstraße 55</Street><Postcode>D-4300</Postcode><City>Essen 1</City><Country>Federal Republic of Germany</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>A new rapid modified micro-neuraminidase-inhibition test (ESSEN-NIT) has recently been described. This test was originally devised to facilitate largescale screening for serum antibodies to influenza virus neuraminidase. It was shown that this test yielded results comparable to those obtained with the WHO standard procedure.</Para><Para>Our report presents data on the comparison between the WHO method and the ESSEN-NIT with respect to their capability in detecting antigenic differences in neuraminidase of various strains of influenza A viruses belonging to the H3N2 subtype family. Two antiserums against the N2 antigens of the A/Hongkong/1/68 (X15HK) and of the A/Port Chalmers/1/73 (X42) recombinant strains were used in characterization experiments. The results obtained indicate that the ESSEN-NIT is at least as sensitive in detecting antigenic variations of neuraminidase as the WHO standard procedure. The drift of the N2 enzyme which occurred as early as 1969 and is known to continue to date, could be clearly demonstrated. Major and minor antigenic changes of neuraminidase in representative influenza isolates were equally well detected by both assays.</Para><Para>The ESSEN-NIT offers the advantage of speed, economy, and computerized evalutaion of data.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Jürgen</namePart><namePart type="family">Werner</namePart><affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Olaf</namePart><namePart type="family">Thraenhart</namePart><affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ernst</namePart><namePart type="family">Kuwert</namePart><affiliation>Institut für Medizinische Virologie und Immunologie, Universität Essen-Gesamthochschule, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateIssued encoding="w3cdtf">1978-11-01</dateIssued><copyrightDate encoding="w3cdtf">1978</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A new rapid modified micro-neuraminidase-inhibition test (ESSEN-NIT) has recently been described. This test was originally devised to facilitate largescale screening for serum antibodies to influenza virus neuraminidase. It was shown that this test yielded results comparable to those obtained with the WHO standard procedure. Our report presents data on the comparison between the WHO method and the ESSEN-NIT with respect to their capability in detecting antigenic differences in neuraminidase of various strains of influenza A viruses belonging to the H3N2 subtype family. Two antiserums against the N2 antigens of the A/Hongkong/1/68 (X15HK) and of the A/Port Chalmers/1/73 (X42) recombinant strains were used in characterization experiments. The results obtained indicate that the ESSEN-NIT is at least as sensitive in detecting antigenic variations of neuraminidase as the WHO standard procedure. The drift of the N2 enzyme which occurred as early as 1969 and is known to continue to date, could be clearly demonstrated. Major and minor antigenic changes of neuraminidase in representative influenza isolates were equally well detected by both assays. The ESSEN-NIT offers the advantage of speed, economy, and computerized evalutaion of data.</abstract><relatedItem type="host"><titleInfo><title>Medical Microbiology and Immunology</title></titleInfo><titleInfo type="abbreviated"><title>Med Microbiol Immunol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1978-11-01</dateIssued><copyrightDate encoding="w3cdtf">1978</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Immunology</topic><topic>Medical Microbiology</topic></subject><identifier type="ISSN">0300-8584</identifier><identifier type="eISSN">1432-1831</identifier><identifier type="JournalID">430</identifier><identifier type="IssueArticleCount">33</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>1978</date><detail type="volume"><number>166</number><caption>vol.</caption></detail><detail type="issue"><number>1-4</number><caption>no.</caption></detail><extent unit="pages"><start>165</start><end>171</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1978</recordOrigin></recordInfo></relatedItem><identifier type="istex">34F0F6AB9F0A0EB1B5B5A7D8B5F301EFBDBA519A</identifier><identifier type="ark">ark:/67375/1BB-BS9C2SWL-S</identifier><identifier type="DOI">10.1007/BF02121146</identifier><identifier type="ArticleID">BF02121146</identifier><identifier type="ArticleID">Art20</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1978</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 1978</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-BS9C2SWL-S/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B08 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000B08 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:34F0F6AB9F0A0EB1B5B5A7D8B5F301EFBDBA519A
|texte= Detection of antigenic variation in influenza virus neuraminidase by the ESSEN-NIT and the WHO standard procedure
}}
| This area was generated with Dilib version V0.6.33. |  |