Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class
Identifieur interne : 000A86 ( Istex/Corpus ); précédent : 000A85; suivant : 000A87Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class
Auteurs : R. Stachan ; J. DrescherSource :
- Journal of Virological Methods [ 0166-0934 ] ; 1987.
English descriptors
- Teeft :
- Acta pathol, Additional method, Antibody, Antibody concentration, Antibody concentrations, Antibody dilution, Antibody molecule, Antibody molecules, Antibody preparation, Antibody preparations, Antigenic, Antigenic determinants, Aqueous uranyl acetate solution, Binding avidity, Colloidal gold, Corresponding concentration, Corresponding values, Dilution, Drescher, Electron microscope, Electron microscopically, Electron microscopy, Gold particle, Gold particles, Influenza, Influenza virus, Influenza virus antibody, Influenza virus hemagglutinin, Molecule, Monoclonal, Monoclonal antibodies, Particle counts, Pattern test, Protein content, Room temperature, Screen magnification, Test sample, Twofold dilutions, Unbound, Unbound antibody, Unbound antibody molecules, Viral, Viral antigens, Virus, Virus particle, Virus particles, Virus surface.
Abstract
Abstract: A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.
Url:
DOI: 10.1016/0166-0934(87)90123-6
Links to Exploration step
ISTEX:A8961656933BB06B181F1FA46783DFC163B2B7D4Le document en format XML
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Drescher</name><affiliation><mods:affiliation>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987">1987</date><biblScope unit="volume">18</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="179">179</biblScope><biblScope unit="page" to="192">192</biblScope></imprint><idno type="ISSN">0166-0934</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acta pathol</term><term>Additional method</term><term>Antibody</term><term>Antibody concentration</term><term>Antibody concentrations</term><term>Antibody dilution</term><term>Antibody molecule</term><term>Antibody molecules</term><term>Antibody preparation</term><term>Antibody preparations</term><term>Antigenic</term><term>Antigenic determinants</term><term>Aqueous uranyl acetate solution</term><term>Binding avidity</term><term>Colloidal gold</term><term>Corresponding concentration</term><term>Corresponding values</term><term>Dilution</term><term>Drescher</term><term>Electron microscope</term><term>Electron microscopically</term><term>Electron microscopy</term><term>Gold particle</term><term>Gold particles</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus antibody</term><term>Influenza virus hemagglutinin</term><term>Molecule</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Particle counts</term><term>Pattern test</term><term>Protein content</term><term>Room temperature</term><term>Screen magnification</term><term>Test sample</term><term>Twofold dilutions</term><term>Unbound</term><term>Unbound antibody</term><term>Unbound antibody molecules</term><term>Viral</term><term>Viral antigens</term><term>Virus</term><term>Virus particle</term><term>Virus particles</term><term>Virus surface</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. 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Stachan</name><affiliations><json:string>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</json:string></affiliations></json:item><json:item><name>J. Drescher</name><affiliations><json:string>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Electron microscopy</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Protein A-gold</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Influenza virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Antibody molecule</value></json:item></subject><arkIstex>ark:/67375/6H6-8T58C0BF-H</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.</abstract><qualityIndicators><score>6.933</score><pdfWordCount>3385</pdfWordCount><pdfCharCount>24491</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>14</pdfPageCount><pdfPageSize>432 x 648 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>129</abstractWordCount><abstractCharCount>890</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class</title><pmid><json:string>3429603</json:string></pmid><pii><json:string>0166-0934(87)90123-6</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Journal of Virological Methods</title><language><json:string>unknown</json:string></language><publicationDate>1987</publicationDate><issn><json:string>0166-0934</json:string></issn><pii><json:string>S0166-0934(00)X0142-5</json:string></pii><volume>18</volume><issue>2–3</issue><pages><first>179</first><last>192</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1987</json:string></date><geogName></geogName><orgName><json:string>Biotronik Inc.</json:string><json:string>Institute of Viroiogy</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>S. Moller</json:string><json:string>R. Stachan</json:string><json:string>T. Table</json:string><json:string>V. Ehrhardt</json:string><json:string>C. Ellguth</json:string><json:string>A. Bottcher</json:string></persName><placeName><json:string>Heidelberg</json:string><json:string>Brazil</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Cremer and Riggs, 1979</json:string><json:string>Drescher and Desselberger, 1976</json:string><json:string>Breschkin et al., 1981</json:string><json:string>Drescher, 1957</json:string><json:string>Drescher et al.</json:string><json:string>Laver, 1960</json:string><json:string>Frankel and Gerhard, 1979</json:string><json:string>Best et al. (1969)</json:string><json:string>Rornantt and Romano, 1977</json:string><json:string>Worisberger and Rosset ( 1977)</json:string><json:string>Horisberger, 1979</json:string><json:string>Laver et al. (1974)</json:string><json:string>Flik, 1983</json:string><json:string>Drescher et al.. 1987</json:string><json:string>Fazekas and Webster, 1961</json:string><json:string>Palmer et al.</json:string><json:string>Lubeck and Gerhard, 1981</json:string><json:string>Horisberger and Rosset (1977)</json:string><json:string>Miiller et al.</json:string><json:string>Pavan et al.. 1987</json:string><json:string>Freundlich, 1909</json:string><json:string>Drescher et al., 1987</json:string><json:string>Roman0 and Romano, 1977</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-8T58C0BF-H</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string><json:string>2 - biotechnology & applied microbiology</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>1987</publicationDate><copyrightDate>1987</copyrightDate><doi><json:string>10.1016/0166-0934(87)90123-6</json:string></doi><id>A8961656933BB06B181F1FA46783DFC163B2B7D4</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8T58C0BF-H/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8T58C0BF-H/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-8T58C0BF-H/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1987</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class</title><author xml:id="author-0000"><persName><forename type="first">R.</forename><surname>Stachan</surname></persName><note type="correspondence"><p>Correspondence to: R. Stachan, Institute of Virology, Medical School of Hannover, Konstanty Gutschow-Strasse, 8. D-3000 Hannover/Kleefeld, F.R.G..</p></note><affiliation>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">J.</forename><surname>Drescher</surname></persName><affiliation>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</affiliation></author><idno type="istex">A8961656933BB06B181F1FA46783DFC163B2B7D4</idno><idno type="ark">ark:/67375/6H6-8T58C0BF-H</idno><idno type="DOI">10.1016/0166-0934(87)90123-6</idno><idno type="PII">0166-0934(87)90123-6</idno></analytic><monogr><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="pISSN">0166-0934</idno><idno type="PII">S0166-0934(00)X0142-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987"></date><biblScope unit="volume">18</biblScope><biblScope unit="issue">2–3</biblScope><biblScope unit="page" from="179">179</biblScope><biblScope unit="page" to="192">192</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1987</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Electron microscopy</term></item><item><term>Protein A-gold</term></item><item><term>Influenza virus</term></item><item><term>Antibody molecule</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1987">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8T58C0BF-H/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VIRMET</jid><aid>87901236</aid><ce:pii>0166-0934(87)90123-6</ce:pii><ce:doi>10.1016/0166-0934(87)90123-6</ce:doi><ce:copyright type="unknown" year="1987"></ce:copyright></item-info><head><ce:title>Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class</ce:title><ce:author-group><ce:author><ce:given-name>R.</ce:given-name><ce:surname>Stachan</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Drescher</ce:surname></ce:author><ce:affiliation><ce:textfn>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: R. Stachan, Institute of Virology, Medical School of Hannover, Konstanty Gutschow-Strasse, 8. D-3000 Hannover/Kleefeld, F.R.G..</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="10" month="7" year="1987"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Electron microscopy</ce:text></ce:keyword><ce:keyword><ce:text>Protein A-gold</ce:text></ce:keyword><ce:keyword><ce:text>Influenza virus</ce:text></ce:keyword><ce:keyword><ce:text>Antibody molecule</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Electron microscopic method for determining the concentration of influenza virus antihemagglutinin antibodies of the IgG class</title></titleInfo><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Stachan</namePart><affiliation>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</affiliation><description>Correspondence to: R. Stachan, Institute of Virology, Medical School of Hannover, Konstanty Gutschow-Strasse, 8. D-3000 Hannover/Kleefeld, F.R.G..</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Drescher</namePart><affiliation>Institute of Virology, Medical School of Hannover, Hannover, F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1987</dateIssued><copyrightDate encoding="w3cdtf">1987</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A novel technique for the determination of the concentration of influenza virus antihemagglutinin antibody of the IgG class is described which is based on the following principle: graded doses of purified antibody are allowed to react with homologous virus, resulting in binding of a portion of antibody. The virus-bound antibody is electron microscopically counted after labeling with protein A-gold. Since Freundlich's λ-formula is valid for describing the relationship between antibody bound per virus particle and the corresponding concentration of unbound antibody, the values of bound antibody recorded can be used for calculating the concentration of unbound antibody. The antibody concentration of the test sample is obtained as sum of bound and unbound antibody. The antibody concentrations measured by use of this technique agreed well with chemically determined values.</abstract><subject><genre>Keywords</genre><topic>Electron microscopy</topic><topic>Protein A-gold</topic><topic>Influenza virus</topic><topic>Antibody molecule</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Virological Methods</title></titleInfo><titleInfo type="abbreviated"><title>VIRMET</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1987</dateIssued></originInfo><identifier type="ISSN">0166-0934</identifier><identifier type="PII">S0166-0934(00)X0142-5</identifier><part><date>1987</date><detail type="volume"><number>18</number><caption>vol.</caption></detail><detail type="issue"><number>2–3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>77</start><end>206</end></extent><extent unit="pages"><start>179</start><end>192</end></extent></part></relatedItem><identifier type="istex">A8961656933BB06B181F1FA46783DFC163B2B7D4</identifier><identifier type="ark">ark:/67375/6H6-8T58C0BF-H</identifier><identifier type="DOI">10.1016/0166-0934(87)90123-6</identifier><identifier type="PII">0166-0934(87)90123-6</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-8T58C0BF-H/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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