Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides
Identifieur interne : 000A17 ( Istex/Corpus ); précédent : 000A16; suivant : 000A18Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides
Auteurs : M. Ochoa ; J. Bárcena ; S. De La Luna ; J. A. Melero ; A. R. Douglas ; A. Nieto ; J. Ortín ; J. J. Skehel ; A. PortelaSource :
- Virus Research [ 0168-1702 ] ; 1995.
English descriptors
- Teeft :
- Amino, Amino acids, Antibody, Antigenic, Antigenic areas, Assay, Competitive elisas, Elisa, Epitope, Influenza, Influenza virus, Influenza viruses, Mabs, Molecular weights, Monoclonal, Monoclonal antibodies, Ochoa, Plasmid, Polymerase, Polypeptide, Protein fragments, Protein regions, Syncytial virus, Viral, Viral strains, Virol, Virology, Virus, Virus research.
Abstract
Abstract: Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.
Url:
DOI: 10.1016/0168-1702(95)00039-S
Links to Exploration step
ISTEX:1FD9EB8FD5520D22554D22D1F1CD09AD7B9825DBLe document en format XML
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Fax: +34 (1) 6388206.</mods:affiliation></affiliation><affiliation><mods:affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1FD9EB8FD5520D22554D22D1F1CD09AD7B9825DB</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1016/0168-1702(95)00039-S</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-LMTN2NWD-1/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000A17</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000A17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides</title><author><name sortKey="Ochoa, M" sort="Ochoa, M" uniqKey="Ochoa M" first="M." last="Ochoa">M. Ochoa</name><affiliation><mods:affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Barcena, J" sort="Barcena, J" uniqKey="Barcena J" first="J." last="Bárcena">J. Bárcena</name><affiliation><mods:affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="De La Luna, S" sort="De La Luna, S" uniqKey="De La Luna S" first="S." last="De La Luna">S. De La Luna</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Melero, J A" sort="Melero, J A" uniqKey="Melero J" first="J. A." last="Melero">J. A. Melero</name><affiliation><mods:affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Douglas, A R" sort="Douglas, A R" uniqKey="Douglas A" first="A. R." last="Douglas">A. R. Douglas</name><affiliation><mods:affiliation>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</mods:affiliation></affiliation></author><author><name sortKey="Nieto, A" sort="Nieto, A" uniqKey="Nieto A" first="A." last="Nieto">A. Nieto</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Ortin, J" sort="Ortin, J" uniqKey="Ortin J" first="J." last="Ortín">J. Ortín</name><affiliation><mods:affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Skehel, J J" sort="Skehel, J J" uniqKey="Skehel J" first="J. J." last="Skehel">J. J. Skehel</name><affiliation><mods:affiliation>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</mods:affiliation></affiliation></author><author><name sortKey="Portela, A" sort="Portela, A" uniqKey="Portela A" first="A." last="Portela">A. Portela</name><affiliation><mods:affiliation>Corresponding author. Fax: +34 (1) 6388206.</mods:affiliation></affiliation><affiliation><mods:affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Research</title><title level="j" type="abbrev">VIRUS</title><idno type="ISSN">0168-1702</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="305">305</biblScope><biblScope unit="page" to="315">315</biblScope></imprint><idno type="ISSN">0168-1702</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-1702</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acids</term><term>Antibody</term><term>Antigenic</term><term>Antigenic areas</term><term>Assay</term><term>Competitive elisas</term><term>Elisa</term><term>Epitope</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Mabs</term><term>Molecular weights</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Ochoa</term><term>Plasmid</term><term>Polymerase</term><term>Polypeptide</term><term>Protein fragments</term><term>Protein regions</term><term>Syncytial virus</term><term>Viral</term><term>Viral strains</term><term>Virol</term><term>Virology</term><term>Virus</term><term>Virus research</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>mabs</json:string><json:string>epitope</json:string><json:string>polypeptide</json:string><json:string>viral</json:string><json:string>amino acids</json:string><json:string>antigenic</json:string><json:string>polymerase</json:string><json:string>influenza</json:string><json:string>monoclonal</json:string><json:string>ochoa</json:string><json:string>virol</json:string><json:string>plasmid</json:string><json:string>amino</json:string><json:string>influenza virus</json:string><json:string>virology</json:string><json:string>monoclonal antibodies</json:string><json:string>virus research</json:string><json:string>elisa</json:string><json:string>antigenic areas</json:string><json:string>protein regions</json:string><json:string>influenza viruses</json:string><json:string>assay</json:string><json:string>molecular weights</json:string><json:string>competitive elisas</json:string><json:string>protein fragments</json:string><json:string>syncytial virus</json:string><json:string>viral strains</json:string><json:string>virus</json:string><json:string>antibody</json:string></teeft></keywords><author><json:item><name>M. Ochoa</name><affiliations><json:string>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</json:string></affiliations></json:item><json:item><name>J. Bárcena</name><affiliations><json:string>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</json:string></affiliations></json:item><json:item><name>S. de la Luna</name><affiliations><json:string>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</json:string></affiliations></json:item><json:item><name>J.A. Melero</name><affiliations><json:string>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</json:string></affiliations></json:item><json:item><name>A.R. Douglas</name><affiliations><json:string>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</json:string></affiliations></json:item><json:item><name>A. Nieto</name><affiliations><json:string>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</json:string></affiliations></json:item><json:item><name>J. Ortín</name><affiliations><json:string>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</json:string></affiliations></json:item><json:item><name>J.J. Skehel</name><affiliations><json:string>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</json:string></affiliations></json:item><json:item><name>A. Portela</name><affiliations><json:string>Corresponding author. Fax: +34 (1) 6388206.</json:string><json:string>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Influenza virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Monoclonal antibody</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Polymerase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Antigenic variation</value></json:item></subject><arkIstex>ark:/67375/6H6-LMTN2NWD-1</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>199</abstractWordCount><abstractCharCount>1302</abstractCharCount><keywordCount>4</keywordCount><score>9.388</score><pdfWordCount>5492</pdfWordCount><pdfCharCount>23910</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>461 x 677 pts</pdfPageSize></qualityIndicators><title>Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides</title><pmid><json:string>8533465</json:string></pmid><pii><json:string>0168-1702(95)00039-S</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Virus Research</title><language><json:string>unknown</json:string></language><publicationDate>1995</publicationDate><issn><json:string>0168-1702</json:string></issn><pii><json:string>S0168-1702(00)X0009-3</json:string></pii><volume>37</volume><issue>3</issue><pages><first>305</first><last>315</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>35S</json:string><json:string>1995</json:string></date><geogName></geogName><orgName><json:string>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW</json:string><json:string>Ministerio de Educacidn y Ciencia</json:string><json:string>Turkey/Wisconsin</json:string><json:string>Grant SC1</json:string><json:string>Grant BIO92</json:string><json:string>Comisidn Interministerial</json:string></orgName><orgName_funder><json:string>Grant SC1</json:string><json:string>Grant BIO92</json:string><json:string>Comisidn Interministerial</json:string></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName><json:string>Singapore</json:string><json:string>Fontainebleau</json:string><json:string>Australia</json:string><json:string>Madrid</json:string><json:string>Puerto Rico</json:string><json:string>Philippines</json:string><json:string>Rostock</json:string><json:string>Spain</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Nieto et al., 1992</json:string><json:string>Herlocher et al., 1992</json:string><json:string>Okazaki et al., 1989</json:string><json:string>Gorman et al., 1990</json:string><json:string>Ochoa et al.</json:string><json:string>Yamashita et al., 1989</json:string><json:string>Fauquier et al., 1991</json:string><json:string>Gorman et al.., 1990</json:string><json:string>Ziegler et al., 1995</json:string><json:string>Garcla et al., 1993</json:string><json:string>M. Ochoa et aL</json:string><json:string>Garcla-Barreno et al., 1989</json:string><json:string>Akkina, 1990</json:string><json:string>Chomel et al., 1989</json:string><json:string>Bfircena et al., 1994</json:string><json:string>Rosenberg et al., 1987</json:string><json:string>M. Ochoa et al.</json:string><json:string>A k k i n a , 1990</json:string><json:string>Kawaoka et al., 1989</json:string><json:string>Hiramatsu et al., 1992</json:string><json:string>Williams et al., 1988</json:string><json:string>Walls et al., 1986</json:string><json:string>Portela et al., 1992</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-LMTN2NWD-1</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Cancer Research</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious Diseases</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>1995</publicationDate><copyrightDate>1995</copyrightDate><doi><json:string>10.1016/0168-1702(95)00039-S</json:string></doi><id>1FD9EB8FD5520D22554D22D1F1CD09AD7B9825DB</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LMTN2NWD-1/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LMTN2NWD-1/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-LMTN2NWD-1/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1995</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Research paper</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides</title><author xml:id="author-0000"><persName><forename type="first">M.</forename><surname>Ochoa</surname></persName><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation></author><author xml:id="author-0001"><persName><forename type="first">J.</forename><surname>Bárcena</surname></persName><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation></author><author xml:id="author-0002"><persName><forename type="first">S.</forename><surname>de la Luna</surname></persName><affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</affiliation></author><author xml:id="author-0003"><persName><forename type="first">J.A.</forename><surname>Melero</surname></persName><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation></author><author xml:id="author-0004"><persName><forename type="first">A.R.</forename><surname>Douglas</surname></persName><affiliation>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</affiliation></author><author xml:id="author-0005"><persName><forename type="first">A.</forename><surname>Nieto</surname></persName><affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</affiliation></author><author xml:id="author-0006"><persName><forename type="first">J.</forename><surname>Ortín</surname></persName><affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</affiliation></author><author xml:id="author-0007"><persName><forename type="first">J.J.</forename><surname>Skehel</surname></persName><affiliation>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</affiliation></author><author xml:id="author-0008"><persName><forename type="first">A.</forename><surname>Portela</surname></persName><affiliation>Corresponding author. Fax: +34 (1) 6388206.</affiliation><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation></author><idno type="istex">1FD9EB8FD5520D22554D22D1F1CD09AD7B9825DB</idno><idno type="ark">ark:/67375/6H6-LMTN2NWD-1</idno><idno type="DOI">10.1016/0168-1702(95)00039-S</idno><idno type="PII">0168-1702(95)00039-S</idno></analytic><monogr><title level="j">Virus Research</title><title level="j" type="abbrev">VIRUS</title><idno type="pISSN">0168-1702</idno><idno type="PII">S0168-1702(00)X0009-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995"></date><biblScope unit="volume">37</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="305">305</biblScope><biblScope unit="page" to="315">315</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1995</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Influenza virus</term></item><item><term>Monoclonal antibody</term></item><item><term>Polymerase</term></item><item><term>Antigenic variation</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-03-24">Modified</change><change when="1995">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LMTN2NWD-1/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VIRUS</jid><aid>9500039S</aid><ce:pii>0168-1702(95)00039-S</ce:pii><ce:doi>10.1016/0168-1702(95)00039-S</ce:doi><ce:copyright type="unknown" year="1995"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Research paper</ce:textfn></ce:dochead><ce:title>Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides</ce:title><ce:author-group><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Ochoa</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Bárcena</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>de la Luna</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.A.</ce:given-name><ce:surname>Melero</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.R.</ce:given-name><ce:surname>Douglas</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Nieto</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Ortín</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.J.</ce:given-name><ce:surname>Skehel</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Portela</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Corresponding author. Fax: +34 (1) 6388206.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="8" month="2" year="1995"></ce:date-received><ce:date-revised day="24" month="3" year="1995"></ce:date-revised><ce:date-accepted day="24" month="3" year="1995"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in <ce:italic>E. coli</ce:italic> is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Influenza virus</ce:text></ce:keyword><ce:keyword><ce:text>Monoclonal antibody</ce:text></ce:keyword><ce:keyword><ce:text>Polymerase</ce:text></ce:keyword><ce:keyword><ce:text>Antigenic variation</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Ochoa</namePart><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Bárcena</namePart><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">de la Luna</namePart><affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.A.</namePart><namePart type="family">Melero</namePart><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.R.</namePart><namePart type="family">Douglas</namePart><affiliation>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Nieto</namePart><affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Ortín</namePart><affiliation>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.J.</namePart><namePart type="family">Skehel</namePart><affiliation>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Portela</namePart><affiliation>Corresponding author. Fax: +34 (1) 6388206.</affiliation><affiliation>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued><dateModified encoding="w3cdtf">1995-03-24</dateModified><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.</abstract><note type="content">Section title: Research paper</note><subject><genre>Keywords</genre><topic>Influenza virus</topic><topic>Monoclonal antibody</topic><topic>Polymerase</topic><topic>Antigenic variation</topic></subject><relatedItem type="host"><titleInfo><title>Virus Research</title></titleInfo><titleInfo type="abbreviated"><title>VIRUS</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued></originInfo><identifier type="ISSN">0168-1702</identifier><identifier type="PII">S0168-1702(00)X0009-3</identifier><part><date>1995</date><detail type="volume"><number>37</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>177</start><end>325</end></extent><extent unit="pages"><start>305</start><end>315</end></extent></part></relatedItem><identifier type="istex">1FD9EB8FD5520D22554D22D1F1CD09AD7B9825DB</identifier><identifier type="ark">ark:/67375/6H6-LMTN2NWD-1</identifier><identifier type="DOI">10.1016/0168-1702(95)00039-S</identifier><identifier type="PII">0168-1702(95)00039-S</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-LMTN2NWD-1/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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