MONOCLONAL ANTIBODIES FOR THE RAPID DIAGNOSIS OF INFLUENZA A AND B VIRUS INFECTIONS BY IMMUNOFLUORESCENCE
Identifieur interne : 000866 ( Istex/Corpus ); précédent : 000865; suivant : 000867MONOCLONAL ANTIBODIES FOR THE RAPID DIAGNOSIS OF INFLUENZA A AND B VIRUS INFECTIONS BY IMMUNOFLUORESCENCE
Auteurs : J. Mcquillin ; C. R Madeley ; A. P KendalSource :
- The Lancet [ 0140-6736 ] ; 1985.
English descriptors
- Teeft :
- Acceptable quality, Antibody, Antiserum, Baboon kidney cells, Cell cultures, Clin microbiol, Clinical specimens, Human kidney, Immunofluorescence, Indirect immunofluorescence, Influenza, Intranuclear fluorescence, Kidney antigens, Mcquillin, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoclonal hybridoma antibodies, Monoclonal pool, Monoclonal pools, Nasopharyngeal, Nasopharyngeal secretion, Nasopharyngeal secretions, Polyclonal, Polyclonal rabbit antiserum, Preliminary testing, Rapid diagnosis, Rapid virus diagnosis, Reproducible quality, Respiratory syncytial virus, Royal victoria infirmary, Satisfactory results, Secretion, Specific fluorescence, Specific influenza, Sputum specimens, Sublethal dose, Summary introduction, Technical assistance, Urinary excretion, Virus infections, World health organisation.
Abstract
Abstract: Mouse monoclonal antibodies, directed against antigenic sites on influenza A and B viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. The influenza A antibodies were directed against nucleoprotein or matrix protein antigens and the influenza B antibodies against nucleoprotein and haemagglutinin antigens. The influenza A anti-matrix monoclonal antibody was found to give a strong intranuclear particulate fluorescence in normal baboon kidney cells and cells from nasopharyngeal secretions negative for influenza A virus, including those from a patient infected with respiratory syncytial virus. Pools of the remaining monoclonal antibodies gave satisfactory results on 25 specimens from patients with influenza A H1N1 and H3N2 subtypes and 12 from patients with influenza B.
Url:
DOI: 10.1016/S0140-6736(85)90849-9
Links to Exploration step
ISTEX:182802BC0A614194808A18318E427042143870FELe document en format XML
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P Kendal</name><affiliation><mods:affiliation>Influenza Branch, Center for Infectious Disease, Centers for Disease Control, Atlanta GA 30333, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">The Lancet</title><title level="j" type="abbrev">LANCET</title><idno type="ISSN">0140-6736</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985">1985</date><biblScope unit="volume">326</biblScope><biblScope unit="issue">8461</biblScope><biblScope unit="page" from="911">911</biblScope><biblScope unit="page" to="914">914</biblScope></imprint><idno type="ISSN">0140-6736</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0140-6736</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acceptable quality</term><term>Antibody</term><term>Antiserum</term><term>Baboon kidney cells</term><term>Cell cultures</term><term>Clin microbiol</term><term>Clinical specimens</term><term>Human kidney</term><term>Immunofluorescence</term><term>Indirect immunofluorescence</term><term>Influenza</term><term>Intranuclear fluorescence</term><term>Kidney antigens</term><term>Mcquillin</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Monoclonal hybridoma antibodies</term><term>Monoclonal pool</term><term>Monoclonal pools</term><term>Nasopharyngeal</term><term>Nasopharyngeal secretion</term><term>Nasopharyngeal secretions</term><term>Polyclonal</term><term>Polyclonal rabbit antiserum</term><term>Preliminary testing</term><term>Rapid diagnosis</term><term>Rapid virus diagnosis</term><term>Reproducible quality</term><term>Respiratory syncytial virus</term><term>Royal victoria infirmary</term><term>Satisfactory results</term><term>Secretion</term><term>Specific fluorescence</term><term>Specific influenza</term><term>Sputum specimens</term><term>Sublethal dose</term><term>Summary introduction</term><term>Technical assistance</term><term>Urinary excretion</term><term>Virus infections</term><term>World health organisation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Mouse monoclonal antibodies, directed against antigenic sites on influenza A and B viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. The influenza A antibodies were directed against nucleoprotein or matrix protein antigens and the influenza B antibodies against nucleoprotein and haemagglutinin antigens. The influenza A anti-matrix monoclonal antibody was found to give a strong intranuclear particulate fluorescence in normal baboon kidney cells and cells from nasopharyngeal secretions negative for influenza A virus, including those from a patient infected with respiratory syncytial virus. Pools of the remaining monoclonal antibodies gave satisfactory results on 25 specimens from patients with influenza A H1N1 and H3N2 subtypes and 12 from patients with influenza B.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>monoclonal</json:string><json:string>monoclonal antibodies</json:string><json:string>nasopharyngeal</json:string><json:string>influenza</json:string><json:string>nasopharyngeal secretions</json:string><json:string>polyclonal</json:string><json:string>immunofluorescence</json:string><json:string>mcquillin</json:string><json:string>polyclonal rabbit antiserum</json:string><json:string>urinary excretion</json:string><json:string>rapid diagnosis</json:string><json:string>clinical specimens</json:string><json:string>respiratory syncytial virus</json:string><json:string>cell cultures</json:string><json:string>baboon kidney cells</json:string><json:string>monoclonal pools</json:string><json:string>sputum specimens</json:string><json:string>specific influenza</json:string><json:string>secretion</json:string><json:string>antibody</json:string><json:string>nasopharyngeal secretion</json:string><json:string>acceptable quality</json:string><json:string>world health organisation</json:string><json:string>summary introduction</json:string><json:string>preliminary testing</json:string><json:string>sublethal dose</json:string><json:string>satisfactory results</json:string><json:string>monoclonal antibody</json:string><json:string>indirect immunofluorescence</json:string><json:string>intranuclear fluorescence</json:string><json:string>clin microbiol</json:string><json:string>specific fluorescence</json:string><json:string>monoclonal pool</json:string><json:string>reproducible quality</json:string><json:string>royal victoria infirmary</json:string><json:string>technical assistance</json:string><json:string>virus infections</json:string><json:string>human kidney</json:string><json:string>kidney antigens</json:string><json:string>rapid virus diagnosis</json:string><json:string>monoclonal hybridoma antibodies</json:string><json:string>antiserum</json:string></teeft></keywords><author><json:item><name>J Mcquillin</name><affiliations><json:string>Department of Virology, Royal Victoria Infirmary and University of Newcastle upon Tyne, United Kingdom</json:string></affiliations></json:item><json:item><name>C.R Madeley</name><affiliations><json:string>Department of Virology, Royal Victoria Infirmary and University of Newcastle upon Tyne, United Kingdom</json:string></affiliations></json:item><json:item><name>A.P Kendal</name><affiliations><json:string>Influenza Branch, Center for Infectious Disease, Centers for Disease Control, Atlanta GA 30333, U.S.A.</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-JX433JB5-8</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Mouse monoclonal antibodies, directed against antigenic sites on influenza A and B viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. The influenza A antibodies were directed against nucleoprotein or matrix protein antigens and the influenza B antibodies against nucleoprotein and haemagglutinin antigens. The influenza A anti-matrix monoclonal antibody was found to give a strong intranuclear particulate fluorescence in normal baboon kidney cells and cells from nasopharyngeal secretions negative for influenza A virus, including those from a patient infected with respiratory syncytial virus. Pools of the remaining monoclonal antibodies gave satisfactory results on 25 specimens from patients with influenza A H1N1 and H3N2 subtypes and 12 from patients with influenza B.</abstract><qualityIndicators><score>6.363</score><pdfWordCount>2839</pdfWordCount><pdfCharCount>19663</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>4</pdfPageCount><pdfPageSize>607 x 943 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>127</abstractWordCount><abstractCharCount>906</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>MONOCLONAL ANTIBODIES FOR THE RAPID DIAGNOSIS OF INFLUENZA A AND B VIRUS INFECTIONS BY IMMUNOFLUORESCENCE</title><pmid><json:string>2865418</json:string></pmid><pii><json:string>S0140-6736(85)90849-9</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc 3rd Int symp rapid methods and automation in microbiology.</title><language><json:string>unknown</json:string></language><pages><first>236</first><last>240</last></pages></serie><host><title>The Lancet</title><language><json:string>unknown</json:string></language><publicationDate>1985</publicationDate><issn><json:string>0140-6736</json:string></issn><pii><json:string>S0140-6736(00)X6386-6</json:string></pii><volume>326</volume><issue>8461</issue><pages><first>911</first><last>914</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1979</json:string><json:string>1982</json:string><json:string>1940</json:string><json:string>1985</json:string></date><geogName></geogName><orgName><json:string>Department of Occupational Medicine, Laboratory of Industrial Toxicology, and Institute of Microbiology, University of Parma, Italy Mouse</json:string><json:string>Department of Virology, Royal Victoria Infirmary</json:string><json:string>FRANCHINI Institute of Internal Medicine and Nephrology</json:string><json:string>Department of Virology, Royal Victoria Infirmary, Newcastle</json:string><json:string>Tyne University</json:string><json:string>Wellcome Diagnostics</json:string><json:string>World Health Organisation</json:string><json:string>Department of Virology, University of Newcastle</json:string><json:string>Common Cold Research Unit, Harvard Hospital, Salisbury</json:string><json:string>WHO Collaborating Center for Influenza</json:string><json:string>Medical Research Council</json:string><json:string>World Health Organisation</json:string></orgName><orgName_funder><json:string>Medical Research Council</json:string><json:string>World Health Organisation</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Newcastle</json:string><json:string>Hong Kong</json:string><json:string>A. 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The influenza A antibodies were directed against nucleoprotein or matrix protein antigens and the influenza B antibodies against nucleoprotein and haemagglutinin antigens. The influenza A anti-matrix monoclonal antibody was found to give a strong intranuclear particulate fluorescence in normal baboon kidney cells and cells from nasopharyngeal secretions negative for influenza A virus, including those from a patient infected with respiratory syncytial virus. Pools of the remaining monoclonal antibodies gave satisfactory results on 25 specimens from patients with influenza A H1N1 and H3N2 subtypes and 12 from patients with influenza B.</p></abstract></profileDesc><revisionDesc><change when="1985">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-JX433JB5-8/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>LANCET</jid><aid>85908499</aid><ce:pii>S0140-6736(85)90849-9</ce:pii><ce:doi>10.1016/S0140-6736(85)90849-9</ce:doi><ce:copyright type="unknown" year="1985"></ce:copyright></item-info><head><ce:title>MONOCLONAL ANTIBODIES FOR THE RAPID DIAGNOSIS OF INFLUENZA A AND B VIRUS INFECTIONS BY IMMUNOFLUORESCENCE</ce:title><ce:author-group><ce:author><ce:given-name>J</ce:given-name><ce:surname>Mcquillin</ce:surname><ce:cross-ref refid="ART2-AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C.R</ce:given-name><ce:surname>Madeley</ce:surname><ce:cross-ref refid="ART2-AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.P</ce:given-name><ce:surname>Kendal</ce:surname><ce:cross-ref refid="ART2-AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="ART2-AFF1"><ce:label>a</ce:label><ce:textfn>Department of Virology, Royal Victoria Infirmary and University of Newcastle upon Tyne, United Kingdom</ce:textfn></ce:affiliation><ce:affiliation id="ART2-AFF2"><ce:label>b</ce:label><ce:textfn>Influenza Branch, Center for Infectious Disease, Centers for Disease Control, Atlanta GA 30333, U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Mouse monoclonal antibodies, directed against antigenic sites on influenza A and B viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. The influenza A antibodies were directed against nucleoprotein or matrix protein antigens and the influenza B antibodies against nucleoprotein and haemagglutinin antigens. The influenza A anti-matrix monoclonal antibody was found to give a strong intranuclear particulate fluorescence in normal baboon kidney cells and cells from nasopharyngeal secretions negative for influenza A virus, including those from a patient infected with respiratory syncytial virus. Pools of the remaining monoclonal antibodies gave satisfactory results on 25 specimens from patients with influenza A H1N1 and H3N2 subtypes and 12 from patients with influenza B.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>MONOCLONAL ANTIBODIES FOR THE RAPID DIAGNOSIS OF INFLUENZA A AND B VIRUS INFECTIONS BY IMMUNOFLUORESCENCE</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>MONOCLONAL ANTIBODIES FOR THE RAPID DIAGNOSIS OF INFLUENZA A AND B VIRUS INFECTIONS BY IMMUNOFLUORESCENCE</title></titleInfo><name type="personal"><namePart type="given">J</namePart><namePart type="family">Mcquillin</namePart><affiliation>Department of Virology, Royal Victoria Infirmary and University of Newcastle upon Tyne, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.R</namePart><namePart type="family">Madeley</namePart><affiliation>Department of Virology, Royal Victoria Infirmary and University of Newcastle upon Tyne, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.P</namePart><namePart type="family">Kendal</namePart><affiliation>Influenza Branch, Center for Infectious Disease, Centers for Disease Control, Atlanta GA 30333, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued><copyrightDate encoding="w3cdtf">1985</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Mouse monoclonal antibodies, directed against antigenic sites on influenza A and B viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. The influenza A antibodies were directed against nucleoprotein or matrix protein antigens and the influenza B antibodies against nucleoprotein and haemagglutinin antigens. The influenza A anti-matrix monoclonal antibody was found to give a strong intranuclear particulate fluorescence in normal baboon kidney cells and cells from nasopharyngeal secretions negative for influenza A virus, including those from a patient infected with respiratory syncytial virus. Pools of the remaining monoclonal antibodies gave satisfactory results on 25 specimens from patients with influenza A H1N1 and H3N2 subtypes and 12 from patients with influenza B.</abstract><relatedItem type="host"><titleInfo><title>The Lancet</title></titleInfo><titleInfo type="abbreviated"><title>LANCET</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued></originInfo><identifier type="ISSN">0140-6736</identifier><identifier type="PII">S0140-6736(00)X6386-6</identifier><part><date>1985</date><detail type="issue"><title>Originally published as Volume 2, Issue 8461</title></detail><detail type="volume"><number>326</number><caption>vol.</caption></detail><detail type="issue"><number>8461</number><caption>no.</caption></detail><extent unit="issue-pages"><start>907</start><end>964</end></extent><extent unit="pages"><start>911</start><end>914</end></extent></part></relatedItem><identifier type="istex">182802BC0A614194808A18318E427042143870FE</identifier><identifier type="ark">ark:/67375/6H6-JX433JB5-8</identifier><identifier type="DOI">10.1016/S0140-6736(85)90849-9</identifier><identifier type="PII">S0140-6736(85)90849-9</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-JX433JB5-8/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= MONOCLONAL ANTIBODIES FOR THE RAPID DIAGNOSIS OF INFLUENZA A AND B VIRUS INFECTIONS BY IMMUNOFLUORESCENCE
}}
| This area was generated with Dilib version V0.6.33. |  |