Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans
Identifieur interne : 000717 ( Istex/Corpus ); précédent : 000716; suivant : 000718Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans
Auteurs : Claudia Michael ; Andreas M. RizziSource :
- Rapid Communications in Mass Spectrometry [ 0951-4198 ] ; 2015-07-15.
Abstract
Rationale: Quantitative monitoring of changes in the N‐glycome upon disease has gained significance in the context of biomarker discovery. Separation and quantification of isobaric glycan isomers can be attained by using high‐performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI‐MS). Collision‐induced dissociation (CID)‐based fragmentation of separated isobaric glycans is evaluated in respect to its potential of providing fragment ions specific for the linkage positions of terminal sialic acids and the presence of intersecting GlcNAc moieties, respectively. Methods: N‐Glycans were labeled via reductive amination using 12C6‐aniline and 13C6‐aniline as isotope‐coded labeling reagents. The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase. Identification of structural features of separated isobaric isomers was performed by CID‐based tandem mass spectrometry (MS/MS) carried out in a quadrupole time‐of‐flight (QqTOF) or a quadrupole ion‐trap (IT) mass spectrometer. Results: Working in the negative ion mode, new diagnostic CID fragment ions could be found that are indicative for the α2,6‐type linkage of sialic acids. Other diagnostic ions, identified before as being indicative for the substitution of the 6‐antenna, could be confirmed as being of relevance also in the case of aniline labeling. In the positive ion mode, CID fragment ions indicative for the structure of short neutral N‐glycans were identified. Conclusions: One new diagnostic ion specific for the linkage position of the terminal sialic acids and one for the presence of bisecting GlcNAc in N‐glycans were identified. The aniline label introduced for improved relative quantitation in MS1 was found not to significantly alter the CID fragmentation patterns that were reported previously by other authors for unlabeled/reduced glycans or for glycans with more polar labels. Copyright © 2015 John Wiley & Sons, Ltd.
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DOI: 10.1002/rcm.7208
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans</title><author><name sortKey="Michael, Claudia" sort="Michael, Claudia" uniqKey="Michael C" first="Claudia" last="Michael">Claudia Michael</name><affiliation><mods:affiliation>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Rizzi, Andreas M" sort="Rizzi, Andreas M" uniqKey="Rizzi A" first="Andreas M." last="Rizzi">Andreas M. Rizzi</name><affiliation><mods:affiliation>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence to: A. M. Rizzi, Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: andreas.rizzi@univie.ac.at</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:984228D70F6ABD1EBAEE37D5B9BC5B2B55E72F2C</idno><date when="2015" year="2015">2015</date><idno type="doi">10.1002/rcm.7208</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-7L4XZ275-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000717</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000717</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans</title><author><name sortKey="Michael, Claudia" sort="Michael, Claudia" uniqKey="Michael C" first="Claudia" last="Michael">Claudia Michael</name><affiliation><mods:affiliation>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Rizzi, Andreas M" sort="Rizzi, Andreas M" uniqKey="Rizzi A" first="Andreas M." last="Rizzi">Andreas M. Rizzi</name><affiliation><mods:affiliation>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence to: A. M. Rizzi, Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: andreas.rizzi@univie.ac.at</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Rapid Communications in Mass Spectrometry</title><title level="j" type="alt">RAPID COMMUNICATIONS IN MASS SPECTROMETRY</title><idno type="ISSN">0951-4198</idno><idno type="eISSN">1097-0231</idno><imprint><biblScope unit="vol">29</biblScope><biblScope unit="issue">13</biblScope><biblScope unit="page" from="1268">1268</biblScope><biblScope unit="page" to="1278">1278</biblScope><biblScope unit="page-count">11</biblScope><date type="published" when="2015-07-15">2015-07-15</date></imprint><idno type="ISSN">0951-4198</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0951-4198</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Rationale: Quantitative monitoring of changes in the N‐glycome upon disease has gained significance in the context of biomarker discovery. Separation and quantification of isobaric glycan isomers can be attained by using high‐performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI‐MS). Collision‐induced dissociation (CID)‐based fragmentation of separated isobaric glycans is evaluated in respect to its potential of providing fragment ions specific for the linkage positions of terminal sialic acids and the presence of intersecting GlcNAc moieties, respectively. Methods: N‐Glycans were labeled via reductive amination using 12C6‐aniline and 13C6‐aniline as isotope‐coded labeling reagents. The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase. Identification of structural features of separated isobaric isomers was performed by CID‐based tandem mass spectrometry (MS/MS) carried out in a quadrupole time‐of‐flight (QqTOF) or a quadrupole ion‐trap (IT) mass spectrometer. Results: Working in the negative ion mode, new diagnostic CID fragment ions could be found that are indicative for the α2,6‐type linkage of sialic acids. Other diagnostic ions, identified before as being indicative for the substitution of the 6‐antenna, could be confirmed as being of relevance also in the case of aniline labeling. In the positive ion mode, CID fragment ions indicative for the structure of short neutral N‐glycans were identified. Conclusions: One new diagnostic ion specific for the linkage position of the terminal sialic acids and one for the presence of bisecting GlcNAc in N‐glycans were identified. The aniline label introduced for improved relative quantitation in MS1 was found not to significantly alter the CID fragmentation patterns that were reported previously by other authors for unlabeled/reduced glycans or for glycans with more polar labels. Copyright © 2015 John Wiley & Sons, Ltd.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Claudia Michael</name><affiliations><json:string>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</json:string></affiliations></json:item><json:item><name>Andreas M. Rizzi</name><affiliations><json:string>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</json:string><json:string>Correspondence to: A. M. Rizzi, Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.E‐mail:</json:string><json:string>E-mail: andreas.rizzi@univie.ac.at</json:string></affiliations></json:item></author><articleId><json:string>RCM7208</json:string></articleId><arkIstex>ark:/67375/WNG-7L4XZ275-Z</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Rationale: Quantitative monitoring of changes in the N‐glycome upon disease has gained significance in the context of biomarker discovery. Separation and quantification of isobaric glycan isomers can be attained by using high‐performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI‐MS). Collision‐induced dissociation (CID)‐based fragmentation of separated isobaric glycans is evaluated in respect to its potential of providing fragment ions specific for the linkage positions of terminal sialic acids and the presence of intersecting GlcNAc moieties, respectively. Methods: N‐Glycans were labeled via reductive amination using 12C6‐aniline and 13C6‐aniline as isotope‐coded labeling reagents. The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase. Identification of structural features of separated isobaric isomers was performed by CID‐based tandem mass spectrometry (MS/MS) carried out in a quadrupole time‐of‐flight (QqTOF) or a quadrupole ion‐trap (IT) mass spectrometer. Results: Working in the negative ion mode, new diagnostic CID fragment ions could be found that are indicative for the α2,6‐type linkage of sialic acids. Other diagnostic ions, identified before as being indicative for the substitution of the 6‐antenna, could be confirmed as being of relevance also in the case of aniline labeling. In the positive ion mode, CID fragment ions indicative for the structure of short neutral N‐glycans were identified. Conclusions: One new diagnostic ion specific for the linkage position of the terminal sialic acids and one for the presence of bisecting GlcNAc in N‐glycans were identified. The aniline label introduced for improved relative quantitation in MS1 was found not to significantly alter the CID fragmentation patterns that were reported previously by other authors for unlabeled/reduced glycans or for glycans with more polar labels. 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isomeric N‐glycans separated by PGC‐LC</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher ref="https://scientific-publisher.data.istex.fr/ark:/67375/H02-QW5Q88H5-V">Wiley Publishing Ltd</publisher><availability><licence>Copyright © 2015 John Wiley & Sons, Ltd.</licence></availability><date type="published" when="2015-07-15"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans</title><title level="a" type="short">MS/MS study of isomeric N‐glycans separated by PGC‐LC</title><author xml:id="author-0000"><persName><forename type="first">Claudia</forename><surname>Michael</surname></persName><affiliation><orgName type="division">Institute of Analytical Chemistry</orgName><orgName type="institution">University of Vienna</orgName><address><street>Währinger Straße 38</street><postCode>1090</postCode><settlement>Vienna</settlement><country key="AT" xml:lang="en">AUSTRIA</country></address></affiliation></author><author xml:id="author-0001" role="corresp"><persName><forename type="first">Andreas M.</forename><surname>Rizzi</surname></persName><affiliation><orgName type="division">Institute of Analytical Chemistry</orgName><orgName type="institution">University of Vienna</orgName><address><street>Währinger Straße 38</street><postCode>1090</postCode><settlement>Vienna</settlement><country key="AT" xml:lang="en">AUSTRIA</country></address></affiliation></author><idno 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Collision‐induced dissociation (CID)‐based fragmentation of separated isobaric glycans is evaluated in respect to its potential of providing fragment ions specific for the linkage positions of terminal sialic acids and the presence of intersecting GlcNAc moieties, respectively.</p><head>Methods</head><p><hi rend="italic">N</hi>‐Glycans were labeled <hi rend="italic">via</hi> reductive amination using <hi rend="superscript">12</hi>C<hi rend="subscript">6</hi>‐aniline and <hi rend="superscript">13</hi>C<hi rend="subscript">6</hi>‐aniline as isotope‐coded labeling reagents. The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase. Identification of structural features of separated isobaric isomers was performed by CID‐based tandem mass spectrometry (MS/MS) carried out in a quadrupole time‐of‐flight (QqTOF) or a quadrupole ion‐trap (IT) mass spectrometer.</p><head>Results</head><p>Working in the negative ion mode, new diagnostic CID fragment ions could be found that are indicative for the α2,6‐type linkage of sialic acids. Other diagnostic ions, identified before as being indicative for the substitution of the 6‐antenna, could be confirmed as being of relevance also in the case of aniline labeling. In the positive ion mode, CID fragment ions indicative for the structure of short neutral <hi rend="italic">N</hi>‐glycans were identified.</p><head>Conclusions</head><p>One new diagnostic ion specific for the linkage position of the terminal sialic acids and one for the presence of bisecting GlcNAc in <hi rend="italic">N</hi>‐glycans were identified. The aniline label introduced for improved relative quantitation in MS<hi rend="superscript">1</hi> was found not to significantly alter the CID fragmentation patterns that were reported previously by other authors for unlabeled/reduced glycans or for glycans with more polar labels. Copyright © 2015 John Wiley & Sons, Ltd.</p></abstract><textClass><keywords rend="articleCategory"><term>Research Article</term></keywords><keywords rend="tocHeading1"><term>Research Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-7L4XZ275-Z/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:lang="en" xml:id="rcm7208"><header><publicationMeta level="product"><doi>10.1002/(ISSN)1097-0231</doi><issn type="print">0951-4198</issn><issn type="electronic">1097-0231</issn><idGroup><id type="product" value="RCM"></id></idGroup><titleGroup><title type="main" sort="RAPID COMMUNICATIONS IN MASS SPECTROMETRY">Rapid Communications in Mass Spectrometry</title><title type="short">Rapid Commun. Mass Spectrom.</title></titleGroup></publicationMeta><publicationMeta level="part" position="130"><doi>10.1002/rcm.v29.13</doi><copyright ownership="publisher">Copyright © 2015 John Wiley & Sons, Ltd.</copyright><numberingGroup><numbering type="journalVolume" number="29">29</numbering><numbering type="journalIssue">13</numbering></numberingGroup><coverDate startDate="2015-07-15">15 July 2015</coverDate></publicationMeta><publicationMeta level="unit" position="90" type="article" status="forIssue"><doi>10.1002/rcm.7208</doi><idGroup><id type="unit" value="RCM7208"></id><id type="society" value="RCM-15-0027.R1"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2015 John Wiley & Sons, Ltd.</copyright><eventGroup><event type="manuscriptReceived" date="2015-01-21"></event><event type="manuscriptRevised" date="2015-03-30"></event><event type="manuscriptAccepted" date="2015-04-01"></event><event type="xmlCreated" agent="SPi Global" date="2015-04-30"></event><event type="publishedOnlineFinalForm" date="2015-05-25"></event><event type="firstOnline" date="2015-05-25"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.7.5 mode:FullText" date="2016-01-27"></event></eventGroup><numberingGroup><numbering type="pageFirst">1268</numbering><numbering type="pageLast">1278</numbering></numberingGroup><correspondenceTo><lineatedText><line>Correspondence to: A. M. Rizzi, Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.</line><line>E‐mail: <email>andreas.rizzi@univie.ac.at</email></line></lineatedText></correspondenceTo><selfCitationGroup><citation type="self" xml:id="rcm7208-cit-0000"><author><familyName>Michael</familyName>, <givenNames>C.</givenNames></author>, and <author><familyName>Rizzi</familyName>, <givenNames>A. M.</givenNames></author> (<pubYear year="2015">2015</pubYear>), <articleTitle>Tandem mass spectrometry of isomeric aniline‐labeled <i>N</i>‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans</articleTitle>. <journalTitle>Rapid Commun. Mass Spectrom.</journalTitle>, <vol number="29">29</vol>, <pageFirst>1268</pageFirst>–<pageLast>1278</pageLast>. doi: <accessionId ref="info:doi/10.1002/rcm.7208">10.1002/rcm.7208</accessionId>.</citation></selfCitationGroup><linkGroup><link type="toTypesetVersion" href="file:RCM.RCM7208.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Tandem mass spectrometry of isomeric aniline‐labeled <i>N</i>‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans</title><title type="short">MS/MS study of isomeric <i>N</i>‐glycans separated by PGC‐LC</title><title type="shortAuthors">C. Michael and A. M. Rizzi</title></titleGroup><creators><creator creatorRole="author" xml:id="rcm7208-cr-0001" affiliationRef="#rcm7208-aff-0001"><personName><givenNames>Claudia</givenNames><familyName>Michael</familyName></personName></creator><creator creatorRole="author" xml:id="rcm7208-cr-0002" affiliationRef="#rcm7208-aff-0001" corresponding="yes"><personName><givenNames>Andreas M.</givenNames><familyName>Rizzi</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="AT" type="organization" xml:id="rcm7208-aff-0001"><orgDiv>Institute of Analytical Chemistry</orgDiv><orgName>University of Vienna</orgName><address><street>Währinger Straße 38</street><postCode>1090</postCode><city>Vienna</city><country>Austria</country></address></affiliation></affiliationGroup><supportingInformation xml:id="rcm7208-supinf-0001"><supportingInfoItem xml:id="rcm7208-supitem-0001"><mediaResource alt="supplementary data" mimeType="application/vnd.openxmlformats-officedocument.wordprocessingml.document" rendition="webOriginal" href="urn-x:wiley:09514198:media:rcm7208:rcm7208-sup-0001-Supplementary"></mediaResource><caption>Supporting info item</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main"><section xml:id="rcm7208-sec-0001" numbered="no"><title type="main">Rationale</title><p>Quantitative monitoring of changes in the <i>N</i>‐glycome upon disease has gained significance in the context of biomarker discovery. Separation and quantification of isobaric glycan isomers can be attained by using high‐performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI‐MS). Collision‐induced dissociation (CID)‐based fragmentation of separated isobaric glycans is evaluated in respect to its potential of providing fragment ions specific for the linkage positions of terminal sialic acids and the presence of intersecting GlcNAc moieties, respectively.</p></section><section xml:id="rcm7208-sec-0002" numbered="no"><title type="main">Methods</title><p><i>N</i>‐Glycans were labeled <i>via</i> reductive amination using <sup>12</sup>C<sub>6</sub>‐aniline and <sup>13</sup>C<sub>6</sub>‐aniline as isotope‐coded labeling reagents. The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase. Identification of structural features of separated isobaric isomers was performed by CID‐based tandem mass spectrometry (MS/MS) carried out in a quadrupole time‐of‐flight (QqTOF) or a quadrupole ion‐trap (IT) mass spectrometer.</p></section><section xml:id="rcm7208-sec-0003" numbered="no"><title type="main">Results</title><p>Working in the negative ion mode, new diagnostic CID fragment ions could be found that are indicative for the α2,6‐type linkage of sialic acids. Other diagnostic ions, identified before as being indicative for the substitution of the 6‐antenna, could be confirmed as being of relevance also in the case of aniline labeling. In the positive ion mode, CID fragment ions indicative for the structure of short neutral <i>N</i>‐glycans were identified.</p></section><section xml:id="rcm7208-sec-0004" numbered="no"><title type="main">Conclusions</title><p>One new diagnostic ion specific for the linkage position of the terminal sialic acids and one for the presence of bisecting GlcNAc in <i>N</i>‐glycans were identified. The aniline label introduced for improved relative quantitation in MS<sup>1</sup> was found not to significantly alter the CID fragmentation patterns that were reported previously by other authors for unlabeled/reduced glycans or for glycans with more polar labels. Copyright © 2015 John Wiley & Sons, Ltd.</p></section></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>MS/MS study of isomeric N‐glycans separated by PGC‐LC</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans</title></titleInfo><name type="personal"><namePart type="given">Claudia</namePart><namePart type="family">Michael</namePart><affiliation>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Andreas M.</namePart><namePart type="family">Rizzi</namePart><affiliation>Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090, Vienna, Austria</affiliation><affiliation>Correspondence to: A. M. Rizzi, Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria.E‐mail:</affiliation><affiliation>E-mail: andreas.rizzi@univie.ac.at</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2015-07-15</dateIssued><dateCreated encoding="w3cdtf">2015-04-30</dateCreated><dateCaptured encoding="w3cdtf">2015-01-21</dateCaptured><dateValid encoding="w3cdtf">2015-04-01</dateValid><edition>Michael, C., and Rizzi, A. M. (2015), Tandem mass spectrometry of isomeric aniline‐labeled N‐glycans separated on porous graphitic carbon: Revealing the attachment position of terminal sialic acids and structures of neutral glycans. Rapid Commun. Mass Spectrom., 29, 1268–1278. doi: 10.1002/rcm.7208.</edition><copyrightDate encoding="w3cdtf">2015</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract>Rationale: Quantitative monitoring of changes in the N‐glycome upon disease has gained significance in the context of biomarker discovery. Separation and quantification of isobaric glycan isomers can be attained by using high‐performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI‐MS). Collision‐induced dissociation (CID)‐based fragmentation of separated isobaric glycans is evaluated in respect to its potential of providing fragment ions specific for the linkage positions of terminal sialic acids and the presence of intersecting GlcNAc moieties, respectively. Methods: N‐Glycans were labeled via reductive amination using 12C6‐aniline and 13C6‐aniline as isotope‐coded labeling reagents. The differently labeled glycans were merged and separated into various species using a porous graphitic carbon (PGC) stationary phase. Identification of structural features of separated isobaric isomers was performed by CID‐based tandem mass spectrometry (MS/MS) carried out in a quadrupole time‐of‐flight (QqTOF) or a quadrupole ion‐trap (IT) mass spectrometer. Results: Working in the negative ion mode, new diagnostic CID fragment ions could be found that are indicative for the α2,6‐type linkage of sialic acids. Other diagnostic ions, identified before as being indicative for the substitution of the 6‐antenna, could be confirmed as being of relevance also in the case of aniline labeling. In the positive ion mode, CID fragment ions indicative for the structure of short neutral N‐glycans were identified. Conclusions: One new diagnostic ion specific for the linkage position of the terminal sialic acids and one for the presence of bisecting GlcNAc in N‐glycans were identified. The aniline label introduced for improved relative quantitation in MS1 was found not to significantly alter the CID fragmentation patterns that were reported previously by other authors for unlabeled/reduced glycans or for glycans with more polar labels. Copyright © 2015 John Wiley & Sons, Ltd.</abstract><note type="additional physical form">Supporting info item</note><relatedItem type="host"><titleInfo><title>Rapid Communications in Mass Spectrometry</title></titleInfo><titleInfo type="abbreviated"><title>Rapid Commun. 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