Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys
Identifieur interne : 000706 ( Istex/Corpus ); précédent : 000705; suivant : 000707Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys
Auteurs : Brian R. Murphy ; Alicia J. Buckler-White ; William T. London ; Mark H. SnyderSource :
- Vaccine [ 0264-410X ] ; 1989.
English descriptors
- Teeft :
- Amino, Amino acid difference, Amino acid differences, Amino acid sequence, Amino acid sequences, Amino acids, Attenuation, Attenuation phenotype, Avian, Avian influenza, Avian influenza virus, Avian virus, Gene, Genetic basis, Human influenza, Human influenza strains, Human influenza virus, Human virus, Human virus parent, Influenza, Influenza virus, Internal genes, Matrix protein, National institutes, Nucleoprotein, Nucleoprotein gene characterization, Nucleotide, Nucleotide sequence, Phenotype, Proline, Reassortant, Reassortant influenza, Reassortant virus, Reassortant viruses, Reassortants, Replication, Serine, Squirrel, Squirrel monkeys, Structural basis, Surface antigen genes, Vaccine, Virus.
Abstract
Abstract: A reassortant virus possessing RNA segment 7, which codes for the M1 and M2 proteins, of the avian influenza A/Mallard/New York/6750/78 (H2N2) virus and the other seven RNA segments of the human influenza A/Udorn/307/72 (H3N2) virus had been shown previously to be markedly restricted in replication in the respiratory tract of squirrel monkeys. In contrast, a reassortant possessing segment 7 of another avian influenza virus, A/Pintail/Alberta/119/79 (H4N6), and the seven other RNA segments from the A/Udorn/72 virus was not restricted. The nucleotide and deduced amino acid sequence of the RNA segment 7 of each virus was determined to identify the structural basis for the attenuation phenotype specified by RNA segment 7 of the A/Mallard/78 virus. Analysis of the deduced amino acid sequences revealed only two amino acid differences in the M1 protein and one difference in the M2 protein, suggesting that the attenuation phenotype of a reassortant virus possessing segment 7 of the A/Mallard/78 virus may be specified by one to three amino acids. Reassortant viruses possessing RNA segment 6, which codes for the nucleoprotein, of either avian influenza virus and the other seven RNA segments of a human influenza virus were also restricted in replication in squirrel monkeys. A comparison of the deduced amino acid sequences of the two avian nucleopeoteins demonstrated only three amino acid differences indicating that these two avian viruses possess NP genes that are highly related. The high degree of relatedness of both the NP and M proteins of these two avian viruses contrasts with their divergent surface antigens. The structural basis for the attenuation phenotype of the NP gene of the A/Mallard/78 virus is being investigated.
Url:
DOI: 10.1016/0264-410X(89)90283-1
Links to Exploration step
ISTEX:8020350C4CEB03A8714A943F8959DB9D1242CBACLe document en format XML
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Murphy</name><affiliation><mods:affiliation>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</mods:affiliation></affiliation></author><author><name sortKey="Buckler White, Alicia J" sort="Buckler White, Alicia J" uniqKey="Buckler White A" first="Alicia J." last="Buckler-White">Alicia J. Buckler-White</name><affiliation><mods:affiliation>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</mods:affiliation></affiliation></author><author><name sortKey="London, William T" sort="London, William T" uniqKey="London W" first="William T." last="London">William T. London</name><affiliation><mods:affiliation>National Institutes of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA</mods:affiliation></affiliation></author><author><name sortKey="Snyder, Mark H" sort="Snyder, Mark H" uniqKey="Snyder M" first="Mark H." last="Snyder">Mark H. Snyder</name><affiliation><mods:affiliation>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="ISSN">0264-410X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">7</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="557">557</biblScope><biblScope unit="page" to="561">561</biblScope></imprint><idno type="ISSN">0264-410X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid difference</term><term>Amino acid differences</term><term>Amino acid sequence</term><term>Amino acid sequences</term><term>Amino acids</term><term>Attenuation</term><term>Attenuation phenotype</term><term>Avian</term><term>Avian influenza</term><term>Avian influenza virus</term><term>Avian virus</term><term>Gene</term><term>Genetic basis</term><term>Human influenza</term><term>Human influenza strains</term><term>Human influenza virus</term><term>Human virus</term><term>Human virus parent</term><term>Influenza</term><term>Influenza virus</term><term>Internal genes</term><term>Matrix protein</term><term>National institutes</term><term>Nucleoprotein</term><term>Nucleoprotein gene characterization</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Phenotype</term><term>Proline</term><term>Reassortant</term><term>Reassortant influenza</term><term>Reassortant virus</term><term>Reassortant viruses</term><term>Reassortants</term><term>Replication</term><term>Serine</term><term>Squirrel</term><term>Squirrel monkeys</term><term>Structural basis</term><term>Surface antigen genes</term><term>Vaccine</term><term>Virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A reassortant virus possessing RNA segment 7, which codes for the M1 and M2 proteins, of the avian influenza A/Mallard/New York/6750/78 (H2N2) virus and the other seven RNA segments of the human influenza A/Udorn/307/72 (H3N2) virus had been shown previously to be markedly restricted in replication in the respiratory tract of squirrel monkeys. In contrast, a reassortant possessing segment 7 of another avian influenza virus, A/Pintail/Alberta/119/79 (H4N6), and the seven other RNA segments from the A/Udorn/72 virus was not restricted. The nucleotide and deduced amino acid sequence of the RNA segment 7 of each virus was determined to identify the structural basis for the attenuation phenotype specified by RNA segment 7 of the A/Mallard/78 virus. Analysis of the deduced amino acid sequences revealed only two amino acid differences in the M1 protein and one difference in the M2 protein, suggesting that the attenuation phenotype of a reassortant virus possessing segment 7 of the A/Mallard/78 virus may be specified by one to three amino acids. Reassortant viruses possessing RNA segment 6, which codes for the nucleoprotein, of either avian influenza virus and the other seven RNA segments of a human influenza virus were also restricted in replication in squirrel monkeys. A comparison of the deduced amino acid sequences of the two avian nucleopeoteins demonstrated only three amino acid differences indicating that these two avian viruses possess NP genes that are highly related. The high degree of relatedness of both the NP and M proteins of these two avian viruses contrasts with their divergent surface antigens. The structural basis for the attenuation phenotype of the NP gene of the A/Mallard/78 virus is being investigated.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>avian</json:string><json:string>reassortant</json:string><json:string>nucleoprotein</json:string><json:string>attenuation</json:string><json:string>influenza</json:string><json:string>amino</json:string><json:string>reassortant virus</json:string><json:string>nucleotide sequence</json:string><json:string>human influenza</json:string><json:string>reassortants</json:string><json:string>avian virus</json:string><json:string>avian influenza</json:string><json:string>replication</json:string><json:string>reassortant viruses</json:string><json:string>influenza virus</json:string><json:string>avian influenza virus</json:string><json:string>serine</json:string><json:string>amino acid sequences</json:string><json:string>phenotype</json:string><json:string>nucleotide</json:string><json:string>attenuation phenotype</json:string><json:string>proline</json:string><json:string>amino acids</json:string><json:string>human influenza virus</json:string><json:string>squirrel monkeys</json:string><json:string>virus</json:string><json:string>amino acid differences</json:string><json:string>amino acid sequence</json:string><json:string>squirrel</json:string><json:string>gene</json:string><json:string>human virus</json:string><json:string>vaccine</json:string><json:string>internal genes</json:string><json:string>nucleoprotein gene characterization</json:string><json:string>human virus parent</json:string><json:string>national institutes</json:string><json:string>matrix protein</json:string><json:string>structural basis</json:string><json:string>surface antigen genes</json:string><json:string>amino acid difference</json:string><json:string>human influenza strains</json:string><json:string>genetic basis</json:string><json:string>reassortant influenza</json:string></teeft></keywords><author><json:item><name>Brian R. Murphy</name><affiliations><json:string>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</json:string></affiliations></json:item><json:item><name>Alicia J. Buckler-White</name><affiliations><json:string>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</json:string></affiliations></json:item><json:item><name>William T. London</name><affiliations><json:string>National Institutes of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA</json:string></affiliations></json:item><json:item><name>Mark H. Snyder</name><affiliations><json:string>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Influenza viruses</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>membrane proteins</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>attenuation</value></json:item></subject><arkIstex>ark:/67375/6H6-HTKKZK6Z-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A reassortant virus possessing RNA segment 7, which codes for the M1 and M2 proteins, of the avian influenza A/Mallard/New York/6750/78 (H2N2) virus and the other seven RNA segments of the human influenza A/Udorn/307/72 (H3N2) virus had been shown previously to be markedly restricted in replication in the respiratory tract of squirrel monkeys. In contrast, a reassortant possessing segment 7 of another avian influenza virus, A/Pintail/Alberta/119/79 (H4N6), and the seven other RNA segments from the A/Udorn/72 virus was not restricted. The nucleotide and deduced amino acid sequence of the RNA segment 7 of each virus was determined to identify the structural basis for the attenuation phenotype specified by RNA segment 7 of the A/Mallard/78 virus. Analysis of the deduced amino acid sequences revealed only two amino acid differences in the M1 protein and one difference in the M2 protein, suggesting that the attenuation phenotype of a reassortant virus possessing segment 7 of the A/Mallard/78 virus may be specified by one to three amino acids. Reassortant viruses possessing RNA segment 6, which codes for the nucleoprotein, of either avian influenza virus and the other seven RNA segments of a human influenza virus were also restricted in replication in squirrel monkeys. A comparison of the deduced amino acid sequences of the two avian nucleopeoteins demonstrated only three amino acid differences indicating that these two avian viruses possess NP genes that are highly related. The high degree of relatedness of both the NP and M proteins of these two avian viruses contrasts with their divergent surface antigens. The structural basis for the attenuation phenotype of the NP gene of the A/Mallard/78 virus is being investigated.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>273</abstractWordCount><abstractCharCount>1755</abstractCharCount><keywordCount>3</keywordCount><score>8.525</score><pdfWordCount>3525</pdfWordCount><pdfCharCount>22235</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>5</pdfPageCount><pdfPageSize>648 x 864 pts</pdfPageSize></qualityIndicators><title>Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys</title><pmid><json:string>2609730</json:string></pmid><pii><json:string>0264-410X(89)90283-1</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. 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In contrast, a reassortant possessing segment 7 of another avian influenza virus, A/Pintail/Alberta/119/79 (H4N6), and the seven other RNA segments from the A/Udorn/72 virus was not restricted. The nucleotide and deduced amino acid sequence of the RNA segment 7 of each virus was determined to identify the structural basis for the attenuation phenotype specified by RNA segment 7 of the A/Mallard/78 virus. Analysis of the deduced amino acid sequences revealed only two amino acid differences in the M1 protein and one difference in the M2 protein, suggesting that the attenuation phenotype of a reassortant virus possessing segment 7 of the A/Mallard/78 virus may be specified by one to three amino acids. Reassortant viruses possessing RNA segment 6, which codes for the nucleoprotein, of either avian influenza virus and the other seven RNA segments of a human influenza virus were also restricted in replication in squirrel monkeys. A comparison of the deduced amino acid sequences of the two avian nucleopeoteins demonstrated only three amino acid differences indicating that these two avian viruses possess NP genes that are highly related. The high degree of relatedness of both the NP and M proteins of these two avian viruses contrasts with their divergent surface antigens. The structural basis for the attenuation phenotype of the NP gene of the A/Mallard/78 virus is being investigated.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Influenza viruses</term></item><item><term>membrane proteins</term></item><item><term>attenuation</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1989">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-HTKKZK6Z-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>JVAC</jid><aid>89902831</aid><ce:pii>0264-410X(89)90283-1</ce:pii><ce:doi>10.1016/0264-410X(89)90283-1</ce:doi><ce:copyright type="unknown" year="1989"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Paper</ce:textfn></ce:dochead><ce:title>Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys</ce:title><ce:author-group><ce:author><ce:given-name>Brian R.</ce:given-name><ce:surname>Murphy</ce:surname><ce:cross-ref refid="COR1"><ce:sup>†</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Alicia J.</ce:given-name><ce:surname>Buckler-White</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>‡</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>William T.</ce:given-name><ce:surname>London</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>§</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Mark H.</ce:given-name><ce:surname>Snyder</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>∗</ce:label><ce:textfn>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>§</ce:label><ce:textfn>National Institutes of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>†</ce:label><ce:text>To whom correspondence should be addressed</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>‡</ce:label><ce:note-para>Present address: Division of Molecular Virology and Immunology, Georgetown University, Rockville, MD 20852, USA</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="3" month="8" year="1988"></ce:date-received><ce:date-accepted day="11" month="11" year="1988"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A reassortant virus possessing RNA segment 7, which codes for the M1 and M2 proteins, of the avian influenza A/Mallard/New York/6750/78 (H2N2) virus and the other seven RNA segments of the human influenza A/Udorn/307/72 (H3N2) virus had been shown previously to be markedly restricted in replication in the respiratory tract of squirrel monkeys. In contrast, a reassortant possessing segment 7 of another avian influenza virus, A/Pintail/Alberta/119/79 (H4N6), and the seven other RNA segments from the A/Udorn/72 virus was not restricted. The nucleotide and deduced amino acid sequence of the RNA segment 7 of each virus was determined to identify the structural basis for the attenuation phenotype specified by RNA segment 7 of the A/Mallard/78 virus. Analysis of the deduced amino acid sequences revealed only two amino acid differences in the M1 protein and one difference in the M2 protein, suggesting that the attenuation phenotype of a reassortant virus possessing segment 7 of the A/Mallard/78 virus may be specified by one to three amino acids. Reassortant viruses possessing RNA segment 6, which codes for the nucleoprotein, of either avian influenza virus and the other seven RNA segments of a human influenza virus were also restricted in replication in squirrel monkeys. A comparison of the deduced amino acid sequences of the two avian nucleopeoteins demonstrated only three amino acid differences indicating that these two avian viruses possess NP genes that are highly related. The high degree of relatedness of both the NP and M proteins of these two avian viruses contrasts with their divergent surface antigens. The structural basis for the attenuation phenotype of the NP gene of the A/Mallard/78 virus is being investigated.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Influenza viruses</ce:text></ce:keyword><ce:keyword><ce:text>membrane proteins</ce:text></ce:keyword><ce:keyword><ce:text>attenuation</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys</title></titleInfo><name type="personal"><namePart type="given">Brian R.</namePart><namePart type="family">Murphy</namePart><affiliation>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</affiliation><description>To whom correspondence should be addressed</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alicia J.</namePart><namePart type="family">Buckler-White</namePart><affiliation>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</affiliation><description>Present address: Division of Molecular Virology and Immunology, Georgetown University, Rockville, MD 20852, USA</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William T.</namePart><namePart type="family">London</namePart><affiliation>National Institutes of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mark H.</namePart><namePart type="family">Snyder</namePart><affiliation>Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A reassortant virus possessing RNA segment 7, which codes for the M1 and M2 proteins, of the avian influenza A/Mallard/New York/6750/78 (H2N2) virus and the other seven RNA segments of the human influenza A/Udorn/307/72 (H3N2) virus had been shown previously to be markedly restricted in replication in the respiratory tract of squirrel monkeys. In contrast, a reassortant possessing segment 7 of another avian influenza virus, A/Pintail/Alberta/119/79 (H4N6), and the seven other RNA segments from the A/Udorn/72 virus was not restricted. The nucleotide and deduced amino acid sequence of the RNA segment 7 of each virus was determined to identify the structural basis for the attenuation phenotype specified by RNA segment 7 of the A/Mallard/78 virus. Analysis of the deduced amino acid sequences revealed only two amino acid differences in the M1 protein and one difference in the M2 protein, suggesting that the attenuation phenotype of a reassortant virus possessing segment 7 of the A/Mallard/78 virus may be specified by one to three amino acids. Reassortant viruses possessing RNA segment 6, which codes for the nucleoprotein, of either avian influenza virus and the other seven RNA segments of a human influenza virus were also restricted in replication in squirrel monkeys. A comparison of the deduced amino acid sequences of the two avian nucleopeoteins demonstrated only three amino acid differences indicating that these two avian viruses possess NP genes that are highly related. The high degree of relatedness of both the NP and M proteins of these two avian viruses contrasts with their divergent surface antigens. The structural basis for the attenuation phenotype of the NP gene of the A/Mallard/78 virus is being investigated.</abstract><note type="content">Section title: Paper</note><subject><genre>Keywords</genre><topic>Influenza viruses</topic><topic>membrane proteins</topic><topic>attenuation</topic></subject><relatedItem type="host"><titleInfo><title>Vaccine</title></titleInfo><titleInfo type="abbreviated"><title>JVAC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued></originInfo><identifier type="ISSN">0264-410X</identifier><identifier type="PII">S0264-410X(00)X0171-5</identifier><part><date>1989</date><detail type="volume"><number>7</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="issue-pages"><start>481</start><end>584</end></extent><extent unit="pages"><start>557</start><end>561</end></extent></part></relatedItem><identifier type="istex">8020350C4CEB03A8714A943F8959DB9D1242CBAC</identifier><identifier type="ark">ark:/67375/6H6-HTKKZK6Z-V</identifier><identifier type="DOI">10.1016/0264-410X(89)90283-1</identifier><identifier type="PII">0264-410X(89)90283-1</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-HTKKZK6Z-V/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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