Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples
Identifieur interne : 000692 ( Istex/Corpus ); précédent : 000691; suivant : 000693Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples
Auteurs : Chad M. Fuller ; Lina Brodd ; Richard M. Irvine ; Dennis J. Alexander ; Elizabeth W. AldousSource :
- Archives of Virology [ 0304-8608 ] ; 2010-06-01.
Abstract
Abstract: A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls’ eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls’ eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.
Url:
DOI: 10.1007/s00705-010-0632-1
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ISTEX:C80306F31F82FC356D7A9F6F6AB34D590F2B6049Le document en format XML
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A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls’ eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls’ eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Chad M. 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A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls’ eggs. 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TocLevels="0"><ArticleID>632</ArticleID><ArticleDOI>10.1007/s00705-010-0632-1</ArticleDOI><ArticleSequenceNumber>1</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples</ArticleTitle><ArticleCategory>Original Article</ArticleCategory><ArticleFirstPage>817</ArticleFirstPage><ArticleLastPage>823</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2010</Year><Month>3</Month><Day>1</Day></RegistrationDate><Received><Year>2009</Year><Month>10</Month><Day>1</Day></Received><Accepted><Year>2010</Year><Month>3</Month><Day>15</Day></Accepted><OnlineDate><Year>2010</Year><Month>3</Month><Day>16</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Chad</GivenName><GivenName>M.</GivenName><FamilyName>Fuller</FamilyName></AuthorName><Contact><Email>c.fuller@vla.defra.gsi.gov.uk</Email></Contact></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Lina</GivenName><FamilyName>Brodd</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Richard</GivenName><GivenName>M.</GivenName><FamilyName>Irvine</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Dennis</GivenName><GivenName>J.</GivenName><FamilyName>Alexander</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Elizabeth</GivenName><GivenName>W.</GivenName><FamilyName>Aldous</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgName>Veterinary Laboratories Agency</OrgName><OrgAddress><City>Surrey</City><Country Code="GB">UK</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>Guy’s Hospital, Genetics Centre</OrgName><OrgAddress><City>London</City><Country Code="GB">UK</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls’ eggs, were tested. A range of positive and negative clinical samples (<Emphasis Type="Italic">n</Emphasis> = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls’ eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Development of an L gene real-time reverse-transcription PCR assay for the detection of avian paramyxovirus type 1 RNA in clinical samples</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Chad</namePart><namePart type="given">M.</namePart><namePart type="family">Fuller</namePart><affiliation>Veterinary Laboratories Agency, Surrey, UK</affiliation><affiliation>E-mail: c.fuller@vla.defra.gsi.gov.uk</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lina</namePart><namePart type="family">Brodd</namePart><affiliation>Guy’s Hospital, Genetics Centre, London, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Richard</namePart><namePart type="given">M.</namePart><namePart type="family">Irvine</namePart><affiliation>Veterinary Laboratories Agency, Surrey, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dennis</namePart><namePart type="given">J.</namePart><namePart type="family">Alexander</namePart><affiliation>Veterinary Laboratories Agency, Surrey, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Elizabeth</namePart><namePart type="given">W.</namePart><namePart type="family">Aldous</namePart><affiliation>Veterinary Laboratories Agency, Surrey, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Vienna</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">2009-10-01</dateCreated><dateIssued encoding="w3cdtf">2010-06-01</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity of the APMV-1 population with a bias towards the more recent European strains, which had been grown in embryonated fowls’ eggs, were tested. A range of positive and negative clinical samples (n = 350) provided by the National Reference Laboratory and International Reference Laboratory at VLA Weybridge were also tested. Positive clinical material included samples considered representative of lineages 3, 4 and 5 obtained from chickens, ducks, pigeons and partridges. The negative sample population was obtained from chickens, turkeys and ducks. The APMV-1 L gene rRT-PCR gave high relative sensitivity (96.05%) and specificity (98.18%) when compared with virus isolation in embryonated fowls’ eggs. It is proposed that this assay could provide a first-line screening tool for the detection of APMV-1 in clinical samples.</abstract><note>Original Article</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title><subTitle>Official Journal of the Virology Division of the International Union of Microbiological Societies</subTitle></titleInfo><titleInfo type="abbreviated"><title>Arch Virol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2010-06-03</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Infectious Diseases</topic><topic>Medical Microbiology</topic><topic>Virology</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">25</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2010</date><detail type="volume"><number>155</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>817</start><end>823</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 2010</recordOrigin></recordInfo></relatedItem><identifier type="istex">C80306F31F82FC356D7A9F6F6AB34D590F2B6049</identifier><identifier type="ark">ark:/67375/VQC-71WBB8Z8-5</identifier><identifier type="DOI">10.1007/s00705-010-0632-1</identifier><identifier type="ArticleID">632</identifier><identifier type="ArticleID">s00705-010-0632-1</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 2010</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 2010</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-71WBB8Z8-5/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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