Cold-sensitivity of polymerase complexes from human or avian influenza A viruses
Identifieur interne : 000680 ( Istex/Corpus ); précédent : 000679; suivant : 000681Cold-sensitivity of polymerase complexes from human or avian influenza A viruses
Auteurs : Pascale Massin ; Nadia Naffakh ; Sylvie Van Der WerfSource :
- International congress series [ 0531-5131 ] ; 2001.
English descriptors
- Teeft :
- Avian, Avian influenza, Avian influenza viruses, Avian origin, Avian viruses, Cell extracts, Determinant, Human influenza viruses, Human virus, Human viruses, Influenza, Influenza virus, Influenza viruses, International congress series, Massin, Mdck, Mdck cells, Plaque assays, Polymerase, Polymerase complexes, Receptor specificity, Reconstituted rnps, Replication, Virol, Virus.
Abstract
Abstract: Background: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. Methods: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. Results: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. Conclusions: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.
Url:
DOI: 10.1016/S0531-5131(01)00399-5
Links to Exploration step
ISTEX:B3B792E8CF5AD9D09BD63AFE024C516F24E6362FLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</title><author><name sortKey="Massin, Pascale" sort="Massin, Pascale" uniqKey="Massin P" first="Pascale" last="Massin">Pascale Massin</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</mods:affiliation></affiliation></author><author><name sortKey="Naffakh, Nadia" sort="Naffakh, Nadia" uniqKey="Naffakh N" first="Nadia" last="Naffakh">Nadia Naffakh</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</mods:affiliation></affiliation></author><author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: svdwerf@pasteur.fr</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:B3B792E8CF5AD9D09BD63AFE024C516F24E6362F</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1016/S0531-5131(01)00399-5</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-W0TQWL0P-D/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000680</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000680</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</title><author><name sortKey="Massin, Pascale" sort="Massin, Pascale" uniqKey="Massin P" first="Pascale" last="Massin">Pascale Massin</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</mods:affiliation></affiliation></author><author><name sortKey="Naffakh, Nadia" sort="Naffakh, Nadia" uniqKey="Naffakh N" first="Nadia" last="Naffakh">Nadia Naffakh</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</mods:affiliation></affiliation></author><author><name sortKey="Van Der Werf, Sylvie" sort="Van Der Werf, Sylvie" uniqKey="Van Der Werf S" first="Sylvie" last="Van Der Werf">Sylvie Van Der Werf</name><affiliation><mods:affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: svdwerf@pasteur.fr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">International congress series</title><title level="j" type="abbrev">ICS</title><idno type="ISSN">0531-5131</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">1219</biblScope><biblScope unit="supplement">C</biblScope><biblScope unit="page" from="471">471</biblScope><biblScope unit="page" to="477">477</biblScope></imprint><idno type="ISSN">0531-5131</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0531-5131</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Avian</term><term>Avian influenza</term><term>Avian influenza viruses</term><term>Avian origin</term><term>Avian viruses</term><term>Cell extracts</term><term>Determinant</term><term>Human influenza viruses</term><term>Human virus</term><term>Human viruses</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>International congress series</term><term>Massin</term><term>Mdck</term><term>Mdck cells</term><term>Plaque assays</term><term>Polymerase</term><term>Polymerase complexes</term><term>Receptor specificity</term><term>Reconstituted rnps</term><term>Replication</term><term>Virol</term><term>Virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Background: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. Methods: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. Results: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. Conclusions: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>avian</json:string><json:string>polymerase</json:string><json:string>avian viruses</json:string><json:string>influenza</json:string><json:string>virol</json:string><json:string>mdck</json:string><json:string>massin</json:string><json:string>international congress series</json:string><json:string>avian origin</json:string><json:string>influenza viruses</json:string><json:string>mdck cells</json:string><json:string>avian influenza viruses</json:string><json:string>replication</json:string><json:string>determinant</json:string><json:string>polymerase complexes</json:string><json:string>influenza virus</json:string><json:string>avian influenza</json:string><json:string>plaque assays</json:string><json:string>receptor specificity</json:string><json:string>reconstituted rnps</json:string><json:string>cell extracts</json:string><json:string>human influenza viruses</json:string><json:string>human viruses</json:string><json:string>human virus</json:string><json:string>virus</json:string></teeft></keywords><author><json:item><name>Pascale Massin</name><affiliations><json:string>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</json:string></affiliations></json:item><json:item><name>Nadia Naffakh</name><affiliations><json:string>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</json:string></affiliations></json:item><json:item><name>Sylvie van der Werf</name><affiliations><json:string>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</json:string><json:string>E-mail: svdwerf@pasteur.fr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Transcription/replication</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Host-specificity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PB2 protein</value></json:item></subject><arkIstex>ark:/67375/6H6-W0TQWL0P-D</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Background: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. Methods: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. Results: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. Conclusions: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.</abstract><qualityIndicators><score>7.165</score><pdfWordCount>2657</pdfWordCount><pdfCharCount>14757</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>544 x 743 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>209</abstractWordCount><abstractCharCount>1342</abstractCharCount><keywordCount>3</keywordCount></qualityIndicators><title>Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</title><pii><json:string>S0531-5131(01)00399-5</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>International congress series</title><language><json:string>unknown</json:string></language><publicationDate>2001</publicationDate><issn><json:string>0531-5131</json:string></issn><pii><json:string>S0531-5131(00)X0003-9</json:string></pii><volume>1219</volume><pages><first>471</first><last>477</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1968</json:string><json:string>1957</json:string><json:string>1997</json:string><json:string>2001</json:string><json:string>1999</json:string></date><geogName></geogName><orgName><json:string>France Abstract Background</json:string><json:string>Medical Center</json:string><json:string>International Congress Series</json:string><json:string>Ministere</json:string></orgName><orgName_funder><json:string>Ministere</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Ida Rijks</json:string><json:string>Claudine Rousseaux</json:string><json:string>Maryse Tardy-Panit</json:string><json:string>Paris Cedex</json:string><json:string>Hong Kong</json:string><json:string>S. van der Werf</json:string><json:string>ELISA Kit</json:string><json:string>J. Pavlovic</json:string><json:string>Nadia Naffakh</json:string><json:string>Roux</json:string><json:string>Sylvie van der Werf</json:string><json:string>J. Ortin</json:string><json:string>P. Palese</json:string></persName><placeName><json:string>Switzerland</json:string><json:string>Hong Kong</json:string><json:string>Madrid</json:string><json:string>Puerto Rico</json:string><json:string>Rostock</json:string><json:string>York</json:string><json:string>Spain</json:string><json:string>Zurich</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>[15,16]</json:string><json:string>Pleschka et al. [18]</json:string><json:string>[17]</json:string><json:string>[1]</json:string><json:string>[16]</json:string><json:string>P. Massin et al.</json:string><json:string>[15]</json:string><json:string>[2]</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-W0TQWL0P-D</json:string></ark><categories><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1016/S0531-5131(01)00399-5</json:string></doi><id>B3B792E8CF5AD9D09BD63AFE024C516F24E6362F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W0TQWL0P-D/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W0TQWL0P-D/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-W0TQWL0P-D/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©2001 Elsevier Science B.V.</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>2001</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Fig. 1: Plaque assays on human and avian influenza viruses at 37 °C and 33 °C. MDCK cells were infected in duplicate with various dilutions of human A/Puerto-Rico/8/34 (PR8) or A/Paris/908/97 (P908), or avian A/FPV/Rostock/34 (FPV) or A/Mallard/NY/6750/78 (MAL) influenza viruses. For each virus, MDCK cells infected with the same dilution at either 37 °C or 33 °C are shown. Titers were determined from dilutions that gave a minimum of 10 plaques. The mean plaque diameters were determined by the measurement of a minimum of 16 plaques. The results of one representative experiment out of two are shown.</note><note type="content">Fig. 2: Transcription/replication at 37 °C and 33 °C of a viral-like CAT reporter RNA with homospecific or heterospecific polymerase complexes. COS-1 cells were co-transfected in duplicate with pPolI-CAT-RT and four plasmids expressing the PB1, PB2, PA and NP proteins derived either from the PR8, FPV, or HK viruses. Cell extracts were prepared and tested for CAT expression following 48 h of incubation at 37 °C or 33 °C. For a given combination of PB1, PB2, PA and NP, the CAT levels measured at 33 °C were compared to the CAT levels measured at 37°C (100%, dashed line). The results are expressed as percentage values, and as the average±SD of two independent experiments.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</title><author xml:id="author-0000"><persName><forename type="first">Pascale</forename><surname>Massin</surname></persName><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Nadia</forename><surname>Naffakh</surname></persName><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Sylvie</forename><surname>van der Werf</surname></persName><email>svdwerf@pasteur.fr</email><note type="correspondence"><p>Corresponding author. Tel.: +33-1-45-68-87-25; fax: +33-1-40-61-32-41</p></note><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</affiliation></author><idno type="istex">B3B792E8CF5AD9D09BD63AFE024C516F24E6362F</idno><idno type="ark">ark:/67375/6H6-W0TQWL0P-D</idno><idno type="DOI">10.1016/S0531-5131(01)00399-5</idno><idno type="PII">S0531-5131(01)00399-5</idno></analytic><monogr><title level="j">International congress series</title><title level="j" type="abbrev">ICS</title><idno type="pISSN">0531-5131</idno><idno type="PII">S0531-5131(00)X0003-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">1219</biblScope><biblScope unit="supplement">C</biblScope><biblScope unit="page" from="471">471</biblScope><biblScope unit="page" to="477">477</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Background: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. Methods: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. Results: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. Conclusions: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Transcription/replication</term></item><item><term>Host-specificity</term></item><item><term>PB2 protein</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W0TQWL0P-D/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>ICS</jid><aid>424</aid><ce:pii>S0531-5131(01)00399-5</ce:pii><ce:doi>10.1016/S0531-5131(01)00399-5</ce:doi><ce:copyright type="full-transfer" year="2001">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</ce:title><ce:author-group><ce:author><ce:given-name>Pascale</ce:given-name><ce:surname>Massin</ce:surname></ce:author><ce:author><ce:given-name>Nadia</ce:given-name><ce:surname>Naffakh</ce:surname></ce:author><ce:author><ce:given-name>Sylvie</ce:given-name><ce:surname>van der Werf</ce:surname><ce:cross-ref refid="COR1">*</ce:cross-ref><ce:e-address>svdwerf@pasteur.fr</ce:e-address></ce:author><ce:affiliation><ce:textfn>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +33-1-45-68-87-25; fax: +33-1-40-61-32-41</ce:text></ce:correspondence></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para><ce:italic>Background</ce:italic>: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. <ce:italic>Methods</ce:italic>: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. <ce:italic>Results</ce:italic>: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. <ce:italic>Conclusions</ce:italic>: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Transcription/replication</ce:text></ce:keyword><ce:keyword><ce:text>Host-specificity</ce:text></ce:keyword><ce:keyword><ce:text>PB2 protein</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Cold-sensitivity of polymerase complexes from human or avian influenza A viruses</title></titleInfo><name type="personal"><namePart type="given">Pascale</namePart><namePart type="family">Massin</namePart><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nadia</namePart><namePart type="family">Naffakh</namePart><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sylvie</namePart><namePart type="family">van der Werf</namePart><affiliation>Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France</affiliation><affiliation>E-mail: svdwerf@pasteur.fr</affiliation><description>Corresponding author. Tel.: +33-1-45-68-87-25; fax: +33-1-40-61-32-41</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Background: Human influenza A viruses replicate in the upper respiratory tract at about 33 °C, whereas avian viruses replicate in the intestinal tract at a temperature close to 41 °C. Methods: The influence of low temperature on virus growth and RNA replication of avian and human viruses was analyzed in MDCK cells. The influence of temperature on the functional efficiency of homo- or heterospecific polymerase complexes derived from human or avian viruses, was examined by making use of a genetic system for the in vivo reconstitution of functional ribonucleoproteins. Results: Virus growth, RNA replication kinetics as well as the functional efficiency of the polymerase complex were found to be impaired at 33 °C as compared to 37 °C in the case of the avian A/FPV/Rostock/34 or A/Mallard/NY/6750/78 viruses, whereas they were comparable in the case of the human A/Puerto Rico/8/34 virus. The polymerase complex from the human virus A/Hong Kong/156/97 of avian origin exhibited an intermediate cold-sensitivity. The cold-sensitivity of the complexes of avian origin was determined mostly by residue 627 of PB2. Conclusions: Our results suggest that a reduced ability of the polymerase complex of avian viruses to ensure replication of the viral genome at 33 °C could contribute to their inability to grow efficiently in humans.</abstract><note type="content">Fig. 1: Plaque assays on human and avian influenza viruses at 37 °C and 33 °C. MDCK cells were infected in duplicate with various dilutions of human A/Puerto-Rico/8/34 (PR8) or A/Paris/908/97 (P908), or avian A/FPV/Rostock/34 (FPV) or A/Mallard/NY/6750/78 (MAL) influenza viruses. For each virus, MDCK cells infected with the same dilution at either 37 °C or 33 °C are shown. Titers were determined from dilutions that gave a minimum of 10 plaques. The mean plaque diameters were determined by the measurement of a minimum of 16 plaques. The results of one representative experiment out of two are shown.</note><note type="content">Fig. 2: Transcription/replication at 37 °C and 33 °C of a viral-like CAT reporter RNA with homospecific or heterospecific polymerase complexes. COS-1 cells were co-transfected in duplicate with pPolI-CAT-RT and four plasmids expressing the PB1, PB2, PA and NP proteins derived either from the PR8, FPV, or HK viruses. Cell extracts were prepared and tested for CAT expression following 48 h of incubation at 37 °C or 33 °C. For a given combination of PB1, PB2, PA and NP, the CAT levels measured at 33 °C were compared to the CAT levels measured at 37°C (100%, dashed line). The results are expressed as percentage values, and as the average±SD of two independent experiments.</note><subject><genre>Keywords</genre><topic>Transcription/replication</topic><topic>Host-specificity</topic><topic>PB2 protein</topic></subject><relatedItem type="host"><titleInfo><title>International congress series</title></titleInfo><titleInfo type="abbreviated"><title>ICS</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued></originInfo><identifier type="ISSN">0531-5131</identifier><identifier type="PII">S0531-5131(00)X0003-9</identifier><part><date>2001</date><detail type="volume"><number>1219</number><caption>vol.</caption></detail><detail type="supplement"><number>C</number><caption>Suppl.</caption></detail><extent unit="issue-pages"><start>1</start><end>1048</end></extent><extent unit="pages"><start>471</start><end>477</end></extent></part></relatedItem><identifier type="istex">B3B792E8CF5AD9D09BD63AFE024C516F24E6362F</identifier><identifier type="ark">ark:/67375/6H6-W0TQWL0P-D</identifier><identifier type="DOI">10.1016/S0531-5131(01)00399-5</identifier><identifier type="PII">S0531-5131(01)00399-5</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Elsevier Science B.V., ©2001</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-W0TQWL0P-D/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000680 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000680 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:B3B792E8CF5AD9D09BD63AFE024C516F24E6362F
|texte= Cold-sensitivity of polymerase complexes from human or avian influenza A viruses
}}
| This area was generated with Dilib version V0.6.33. |  |