Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS1/HA2 influenza virus protein
Identifieur interne : 000612 ( Istex/Corpus ); précédent : 000611; suivant : 000613Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS1/HA2 influenza virus protein
Auteurs : Innocent N. Mbawuike ; Susan B. Dillon ; Sandra G. Demuth ; Christopher S. Jones ; Thomas R. Cate ; Robert B. CouchSource :
- Vaccine [ 0264-410X ] ; 1994.
English descriptors
- KwdEn :
- Teeft :
- Active immunization, Adoptive transfer, Agents chemother, Alum, Amino acids, Antibody responses, Antibody treatment, Antigenic, Antigenic drift, Braciale, Cell response, Chimeric, Chimeric protein, Clone, Control mice, Cotton rats, Cumulative mortality rates, Cytotoxic, Effector, Effector cells, Ennis, Experiments mice, Further evaluation, Haplotype dependence, Immune, Immunization, Immunodominant epitope, Immunol, Inbred, Inbred mice, Infection, Influenza, Influenza nucleoprotein, Influenza pneumonia, Influenza vaccines, Influenza virus, Influenza virus challenge, Influenza virus infection, Influenza viruses, Last injection, Ldso, Lethal dose, Lung virus, Lung virus infection, Lymphocyte, Lymphocyte clones, Mabs, Major histocompatibility, Maximal release, Mbawuike, Mdck cells, Microneutralization test, Mouse, Natl acad, Neutralizing, Neutralizing activity, Neutralizing antibody, Ntab, Nucleoprotein, Outbred, Outbred mice, Outbred swiss, Passive transfer, Peptide, Present results, Present study, Previous studies, Protection rate, Protective efficacy, Protective immunity, Protein, Proteinimmunized mice, Recombinant, Respiratory syncytial virus, Significant protection, Spleen cells, Splenic lymphocytes, Squirrel monkeys, Stimulator cells, Subunit, Swiss mice, Syncytial virus, Synthetic peptides, Target cells, Tract infection, Unimmunized mice, Unpublished results, Vaccine, Viral, Viral clearance, Virol, Virus, Virus challenge, Virus infection, Virus infections, Virus titres, Vivo depletion.
Abstract
Influenza A/PR/8/34-derived chimeric (D) protein (SK&F 106160) composed of the first 81 amino acids (aa) of NS1 fused to the conserved 157 C-terminal aa of HA2 (NS11-81-HA 265-222) was previously shown to induce H-2d-restricted protective cytotoxic T-lymphocyte (CTL) immunity in inbred mice. However, D protein, like other small peptides, exhibited haplotype dependence and was not immunogenic in H-2b and H-2K mice. A potential use of this antigen in humans and the role of T cells in any protection were evaluated in outbred Swiss and inbred CBF6F1 (H-2d/b) mice. Mice immunized with D protein and challenged by small-particle aerosol with a lethal dose of influenza virus were significantly protected against mortality from influenza A/HINI and A/H2N2 (p < 0.05- < 0.0000001), but not from A/H3N2 and influenza B viruses when compared with control mice. D protein did not induce serum virus-neutralizing antibody but caused virus to be cleared faster in immunized mice. Protection was long-lasting. In vivo depletion of either Lyt2 (CD8+) or L3T4 (CD4+) T cells with monoclonal antibodies led to abrogation of in vitro-generated CTL activity in CF6F1 mice and significant reduction in the protective efficacy of D protein against virus challenge in both Swiss and CF6F1 mice. These results suggest that protection was mediated by CD8+ and/or CD4+ cells and not antibody. Thus D protein, via a conserved sequence on the HA2 polypeptide, has the potential to induce partially cross-reactive CTL that may protect against influenza virus disease in humans.
Url:
DOI: 10.1016/S0264-410X(94)80063-6
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Influenza A/PR/8/34-derived chimeric (D) protein (SK&F 106160) composed of the first 81 amino acids (aa) of NS1 fused to the conserved 157 C-terminal aa of HA2 (NS11-81-HA 265-222) was previously shown to induce H-2d-restricted protective cytotoxic T-lymphocyte (CTL) immunity in inbred mice. However, D protein, like other small peptides, exhibited haplotype dependence and was not immunogenic in H-2b and H-2K mice. A potential use of this antigen in humans and the role of T cells in any protection were evaluated in outbred Swiss and inbred CBF6F1 (H-2d/b) mice. Mice immunized with D protein and challenged by small-particle aerosol with a lethal dose of influenza virus were significantly protected against mortality from influenza A/HINI and A/H2N2 (p < 0.05- < 0.0000001), but not from A/H3N2 and influenza B viruses when compared with control mice. D protein did not induce serum virus-neutralizing antibody but caused virus to be cleared faster in immunized mice. Protection was long-lasting. In vivo depletion of either Lyt2 (CD8+) or L3T4 (CD4+) T cells with monoclonal antibodies led to abrogation of in vitro-generated CTL activity in CF6F1 mice and significant reduction in the protective efficacy of D protein against virus challenge in both Swiss and CF6F1 mice. These results suggest that protection was mediated by CD8+ and/or CD4+ cells and not antibody. Thus D protein, via a conserved sequence on the HA2 polypeptide, has the potential to induce partially cross-reactive CTL that may protect against influenza virus disease in humans.</div>
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<surname>Mbawuike</surname>
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<affiliation>To whom all correspondence should be addressed</affiliation>
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<affiliation>Department of Molecular Virology and Host Defense SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA</affiliation>
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<abstract xml:lang="en"><p>Influenza A/PR/8/34-derived chimeric (D) protein (SK&F 106160) composed of the first 81 amino acids (aa) of NS1 fused to the conserved 157 C-terminal aa of HA2 (NS11-81-HA 265-222) was previously shown to induce H-2d-restricted protective cytotoxic T-lymphocyte (CTL) immunity in inbred mice. However, D protein, like other small peptides, exhibited haplotype dependence and was not immunogenic in H-2b and H-2K mice. A potential use of this antigen in humans and the role of T cells in any protection were evaluated in outbred Swiss and inbred CBF6F1 (H-2d/b) mice. Mice immunized with D protein and challenged by small-particle aerosol with a lethal dose of influenza virus were significantly protected against mortality from influenza A/HINI and A/H2N2 (p < 0.05- < 0.0000001), but not from A/H3N2 and influenza B viruses when compared with control mice. D protein did not induce serum virus-neutralizing antibody but caused virus to be cleared faster in immunized mice. Protection was long-lasting. In vivo depletion of either Lyt2 (CD8+) or L3T4 (CD4+) T cells with monoclonal antibodies led to abrogation of in vitro-generated CTL activity in CF6F1 mice and significant reduction in the protective efficacy of D protein against virus challenge in both Swiss and CF6F1 mice. These results suggest that protection was mediated by CD8+ and/or CD4+ cells and not antibody. Thus D protein, via a conserved sequence on the HA2 polypeptide, has the potential to induce partially cross-reactive CTL that may protect against influenza virus disease in humans.</p>
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<head><ce:title>Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS<ce:inf loc="post">1</ce:inf>
/HA<ce:inf loc="post">2</ce:inf>
influenza virus protein</ce:title>
<ce:author-group><ce:author><ce:given-name>Innocent N.</ce:given-name>
<ce:surname>Mbawuike</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>*</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="cor1"><ce:sup>∞</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Susan B.</ce:given-name>
<ce:surname>Dillon</ce:surname>
<ce:cross-ref refid="aff2"><ce:sup>†</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Sandra G.</ce:given-name>
<ce:surname>Demuth</ce:surname>
<ce:cross-ref refid="aff2"><ce:sup>†</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Christopher S.</ce:given-name>
<ce:surname>Jones</ce:surname>
<ce:cross-ref refid="aff2"><ce:sup>†</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Thomas R.</ce:given-name>
<ce:surname>Cate</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>*</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Robert B.</ce:given-name>
<ce:surname>Couch</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>*</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:affiliation id="aff1"><ce:label>*</ce:label>
<ce:textfn>Acute Viral Respiratory Disease Unit, Influenza Research Center, Department of Microbiology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030-3498, USA</ce:textfn>
</ce:affiliation>
<ce:affiliation id="aff2"><ce:label>†</ce:label>
<ce:textfn>Department of Molecular Virology and Host Defense SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA</ce:textfn>
</ce:affiliation>
<ce:affiliation id="aff3"><ce:label>‡</ce:label>
<ce:textfn>Department of Protein Biochemistry, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA</ce:textfn>
</ce:affiliation>
<ce:correspondence id="cor1"><ce:label>∞</ce:label>
<ce:text>To whom all correspondence should be addressed</ce:text>
</ce:correspondence>
</ce:author-group>
<ce:date-received day="4" month="10" year="1993"></ce:date-received>
<ce:date-revised day="29" month="3" year="1994"></ce:date-revised>
<ce:date-accepted day="30" month="3" year="1994"></ce:date-accepted>
<ce:abstract id="ab1" class="author" xml:lang="en"><ce:abstract-sec><ce:simple-para>Influenza A/PR/8/34-derived chimeric (D) protein (SK&F 106160) composed of the first 81 amino acids (aa) of NS<ce:inf loc="post">1</ce:inf>
fused to the conserved 157 C-terminal aa of HA<ce:inf loc="post">2</ce:inf>
(NS<ce:inf loc="post">11-81-HA 265-222</ce:inf>
) was previously shown to induce H-2<ce:sup loc="post">d</ce:sup>
-restricted protective cytotoxic T-lymphocyte (CTL) immunity in inbred mice. However, D protein, like other small peptides, exhibited haplotype dependence and was not immunogenic in H-2<ce:sup loc="post">b</ce:sup>
and H-2<ce:sup loc="post">K</ce:sup>
mice. A potential use of this antigen in humans and the role of T cells in any protection were evaluated in outbred Swiss and inbred CBF6F<ce:inf loc="post">1</ce:inf>
(H-2<ce:sup loc="post">d/b</ce:sup>
) mice. Mice immunized with D protein and challenged by small-particle aerosol with a lethal dose of influenza virus were significantly protected against mortality from influenza A/HINI and A/H2N2 (p < 0.05- < 0.0000001), but not from A/H3N2 and influenza B viruses when compared with control mice. D protein did not induce serum virus-neutralizing antibody but caused virus to be cleared faster in immunized mice. Protection was long-lasting. In vivo depletion of either Lyt2 (CD8+) or L<ce:inf loc="post">3</ce:inf>
T<ce:inf loc="post">4</ce:inf>
(CD4+) T cells with monoclonal antibodies led to abrogation of <ce:italic>in vitro</ce:italic>
-generated CTL activity in CF6F<ce:inf loc="post">1</ce:inf>
mice and significant reduction in the protective efficacy of D protein against virus challenge in both Swiss and CF6F<ce:inf loc="post">1</ce:inf>
mice. These results suggest that protection was mediated by CD8+ and/or CD4+ cells and not antibody. Thus D protein, via a conserved sequence on the HA<ce:inf loc="post">2</ce:inf>
polypeptide, has the potential to induce partially cross-reactive CTL that may protect against influenza virus disease in humans.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
<ce:keywords class="keyword" xml:lang="en"><ce:section-title>Keywords</ce:section-title>
<ce:keyword><ce:text>Chimeric influenza protein</ce:text>
</ce:keyword>
<ce:keyword><ce:text>influenza A subtype</ce:text>
</ce:keyword>
<ce:keyword><ce:text>cross-protection</ce:text>
</ce:keyword>
</ce:keywords>
</head>
<tail><ce:bibliography><ce:section-title>References</ce:section-title>
<ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>1</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>McMichael</ce:surname>
<ce:given-name>J.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Gotch</ce:surname>
<ce:given-name>F.M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Nobel</ce:surname>
<ce:given-name>G.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Beare</ce:surname>
<ce:given-name>A.S.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cytotoxic T-cell immunity to influenza</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>N. Engl. J. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>309</sb:volume-nr>
</sb:series>
<sb:date>1983</sb:date>
</sb:issue>
<sb:pages><sb:first-page>13</sb:first-page>
<sb:last-page>17</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib2"><ce:label>2</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yap</ce:surname>
<ce:given-name>K.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ada</ce:surname>
<ce:given-name>G.L.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>The recovery of mice from influenza virus infection: adoptive transfer of immunity with immune T lymphocytes</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Scand. J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>7</sb:volume-nr>
</sb:series>
<sb:date>1978</sb:date>
</sb:issue>
<sb:pages><sb:first-page>389</sb:first-page>
<sb:last-page>397</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib3"><ce:label>3</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yap</ce:surname>
<ce:given-name>K.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ada</ce:surname>
<ce:given-name>G.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>McKenzie</ce:surname>
<ce:given-name>I.F.C.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Transfer of specific cytotoxic T lymphocytes protects mice inoculated with influenza virus</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle>
</sb:title>
<sb:volume-nr>273</sb:volume-nr>
</sb:series>
<sb:date>1978</sb:date>
</sb:issue>
<sb:pages><sb:first-page>238</sb:first-page>
<sb:last-page>239</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib4"><ce:label>4</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wells</ce:surname>
<ce:given-name>M.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Albrecht</ce:surname>
<ce:given-name>P.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ennis</ce:surname>
<ce:given-name>F.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Recovery from a viral respiratory tract infection. I. Influenza pneumonia in normal and T deficient mice</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>126</sb:volume-nr>
</sb:series>
<sb:date>1981</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1036</sb:first-page>
<sb:last-page>1041</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib5"><ce:label>5</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wells</ce:surname>
<ce:given-name>M.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ennis</ce:surname>
<ce:given-name>F.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Albrecht</ce:surname>
<ce:given-name>P.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Recovery from a viral respiratory tract infection. II. Passive transfer of immune spleen cells to mice with influenza pneumonia</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>126</sb:volume-nr>
</sb:series>
<sb:date>1981</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1042</sb:first-page>
<sb:last-page>1046</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib6"><ce:label>6</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yewdell</ce:surname>
<ce:given-name>J.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Bennink</ce:surname>
<ce:given-name>J.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Smith</ce:surname>
<ce:given-name>G.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Moss</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:edited-book><sb:book-series><sb:series><sb:title><sb:maintitle>Proc. Natl Acad. Sci. USA</sb:maintitle>
</sb:title>
<sb:volume-nr>82</sb:volume-nr>
</sb:series>
</sb:book-series>
<sb:date>1985</sb:date>
</sb:edited-book>
<sb:pages><sb:first-page>1785</sb:first-page>
<sb:last-page>1789</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib7"><ce:label>7</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yewdell</ce:surname>
<ce:given-name>J.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Hackett</ce:surname>
<ce:given-name>C.J.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>The specificity and function of T lymphocytes induced by influenza A viruses</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Krug</ce:surname>
<ce:given-name>R.</ce:given-name>
</sb:editor>
</sb:editors>
<sb:title><sb:maintitle>The Influenza Viruses</sb:maintitle>
</sb:title>
<sb:date>1989</sb:date>
<sb:publisher><sb:name>Plenum Press</sb:name>
<sb:location>New York</sb:location>
</sb:publisher>
</sb:edited-book>
<sb:pages><sb:first-page>361</sb:first-page>
<sb:last-page>429</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib8"><ce:label>8</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Kees</ce:surname>
<ce:given-name>U.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Hammer</ce:surname>
<ce:given-name>P.H.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Most influenza A virus-specific memory cytotoxic T lymphocytes react with antigenic epitopes associated with internal virus determinants</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>159</sb:volume-nr>
</sb:series>
<sb:date>1984</sb:date>
</sb:issue>
<sb:pages><sb:first-page>365</sb:first-page>
<sb:last-page>377</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib9"><ce:label>9</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Gotch</ce:surname>
<ce:given-name>F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>McMichael</ce:surname>
<ce:given-name>A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Smith</ce:surname>
<ce:given-name>G.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Moss</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Identification of viral molecules recognized by influenza-specific human cytotoxic T-lymphocytes</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>165</sb:volume-nr>
</sb:series>
<sb:date>1987</sb:date>
</sb:issue>
<sb:pages><sb:first-page>406</sb:first-page>
<sb:last-page>416</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib10"><ce:label>10</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yap</ce:surname>
<ce:given-name>K.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ada</ce:surname>
<ce:given-name>G.L.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>An analysis of the effector T cell generation and function in mice exposed to influenza A or Sendai virus</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Immunol. Rev.</sb:maintitle>
</sb:title>
<sb:volume-nr>58</sb:volume-nr>
</sb:series>
<sb:date>1981</sb:date>
</sb:issue>
<sb:pages><sb:first-page>5</sb:first-page>
<sb:last-page>24</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib11"><ce:label>11</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>T.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Yap</ce:surname>
<ce:given-name>K.L.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Role of viral infectivity in the induction of influenza virus specific cytotoxic T-cells</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>147</sb:volume-nr>
</sb:series>
<sb:date>1978</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1236</sb:first-page>
<sb:last-page>1252</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib12"><ce:label>12</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Reiss</ce:surname>
<ce:given-name>C.S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Schulman</ce:surname>
<ce:given-name>J.L.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cellular immune responses of mice to influenza virus vaccines</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>125</sb:volume-nr>
</sb:series>
<sb:date>1980</sb:date>
</sb:issue>
<sb:pages><sb:first-page>2182</sb:first-page>
<sb:last-page>2188</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib13"><ce:label>13</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Armerding</ce:surname>
<ce:given-name>D.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Liehl</ce:surname>
<ce:given-name>E.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Induction of homotypic and heterotypic T-and B-cell immunity with influenza A virus in mice</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Cell. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>60</sb:volume-nr>
</sb:series>
<sb:date>1981</sb:date>
</sb:issue>
<sb:pages><sb:first-page>119</sb:first-page>
<sb:last-page>135</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib14"><ce:label>14</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wiley</ce:surname>
<ce:given-name>D.C.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Wilson</ce:surname>
<ce:given-name>I.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Skehel</ce:surname>
<ce:given-name>J.J.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Structural identification of the antibody binding sites of Hong Kong influenza hemagglutinin and their involvement in antigenic variation</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle>
</sb:title>
<sb:volume-nr>289</sb:volume-nr>
</sb:series>
<sb:date>1981</sb:date>
</sb:issue>
<sb:pages><sb:first-page>373</sb:first-page>
<sb:last-page>378</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib15"><ce:label>15</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Both</ce:surname>
<ce:given-name>G.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Sleigh</ce:surname>
<ce:given-name>M.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Cox</ce:surname>
<ce:given-name>N.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Kendal</ce:surname>
<ce:given-name>A.P.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Antigenic drift in influenza virus H3 hemagglutinin from 1968 to 1980: multiple evolutionary pathways and sequence amino acid changes at key antigenic sites</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Virol.</sb:maintitle>
</sb:title>
<sb:volume-nr>48</sb:volume-nr>
</sb:series>
<sb:date>1983</sb:date>
</sb:issue>
<sb:pages><sb:first-page>52</sb:first-page>
<sb:last-page>60</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib16"><ce:label>16</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Palese</ce:surname>
<ce:given-name>P.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Young</ce:surname>
<ce:given-name>J.F.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Variation of influenza A, B, and C viruses</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Science</sb:maintitle>
</sb:title>
<sb:volume-nr>215</sb:volume-nr>
</sb:series>
<sb:date>1982</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1468</sb:first-page>
<sb:last-page>1473</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib17"><ce:label>17</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Shaw</ce:surname>
<ce:given-name>M.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Lamon</ce:surname>
<ce:given-name>E.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Compass</ce:surname>
<ce:given-name>R.W.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Immunologic studies on the influenza A virus nonstructural protein NS<ce:inf loc="post">1</ce:inf>
</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>156</sb:volume-nr>
</sb:series>
<sb:date>1982</sb:date>
</sb:issue>
<sb:pages><sb:first-page>243</sb:first-page>
<sb:last-page>254</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib18"><ce:label>18</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Torres</ce:surname>
<ce:given-name>J.V.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Wyde</ce:surname>
<ce:given-name>P.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Atassi</ce:surname>
<ce:given-name>M.Z.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cytotoxic T lymphocyte recognition sites on influenza virus hemagglutinin</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Immunol. Lett.</sb:maintitle>
</sb:title>
<sb:volume-nr>19</sb:volume-nr>
</sb:series>
<sb:date>1988</sb:date>
</sb:issue>
<sb:pages><sb:first-page>49</sb:first-page>
<sb:last-page>54</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib19"><ce:label>19</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Hioe</ce:surname>
<ce:given-name>C.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Hinshaw</ce:surname>
<ce:given-name>V.S.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Induction of class II restricted Lyt-2<ce:sup loc="post">+</ce:sup>
cytotoxic T lympohcytes specific for the influenza H5 hemagglutinin</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>142</sb:volume-nr>
</sb:series>
<sb:date>1989</sb:date>
</sb:issue>
<sb:pages><sb:first-page>2482</sb:first-page>
<sb:last-page>2488</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib20"><ce:label>20</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Morrison</ce:surname>
<ce:given-name>L.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Lukacher</ce:surname>
<ce:given-name>A.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>V.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Fan</ce:surname>
<ce:given-name>D.P.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>T.J.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Differences in antigen presentation to MHC class I- and class II-restricted influenza virus-specific cytolytic T lymphocyte clones</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>163</sb:volume-nr>
</sb:series>
<sb:date>1986</sb:date>
</sb:issue>
<sb:pages><sb:first-page>903</sb:first-page>
<sb:last-page>921</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib21"><ce:label>21</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lukacher</ce:surname>
<ce:given-name>A.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Morrison</ce:surname>
<ce:given-name>L.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>V.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Malissen</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>T.J.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Expression of specific cytolytic activity by H-21 region-restricted, influenza virus-specific T lymphocyte clones</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>162</sb:volume-nr>
</sb:series>
<sb:date>1985</sb:date>
</sb:issue>
<sb:pages><sb:first-page>171</sb:first-page>
<sb:last-page>187</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib22"><ce:label>22</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Gould</ce:surname>
<ce:given-name>K.G.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Scotney</ce:surname>
<ce:given-name>H.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Townsend</ce:surname>
<ce:given-name>A.R.M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Bastin</ce:surname>
<ce:given-name>J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Brownlee</ce:surname>
<ce:given-name>G.G.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Mouse H-2<ce:sup loc="post">K</ce:sup>
-restricted cytotoxic T cells recognize antigenic determinants in the HA1 and HA2 subunits of the influenza A/PR/8/34 hemagglutinin</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>166</sb:volume-nr>
</sb:series>
<sb:date>1987</sb:date>
</sb:issue>
<sb:pages><sb:first-page>693</sb:first-page>
<sb:last-page>701</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib23"><ce:label>23</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Zweerink</ce:surname>
<ce:given-name>H.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Askonas</ce:surname>
<ce:given-name>B.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Millican</ce:surname>
<ce:given-name>D.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Courtneige</ce:surname>
<ce:given-name>S.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Skehel</ce:surname>
<ce:given-name>J.J.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cytotoxic T cells to type A influenza virus: viral hemagglutinin induces A strain specificity while infected cells confer cross-reactive cytotoxicity</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Eur. J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>7</sb:volume-nr>
</sb:series>
<sb:date>1977</sb:date>
</sb:issue>
<sb:pages><sb:first-page>630</sb:first-page>
<sb:last-page>635</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib24"><ce:label>24</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yamada</ce:surname>
<ce:given-name>A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ziese</ce:surname>
<ce:given-name>M.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Young</ce:surname>
<ce:given-name>J.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Yamada</ce:surname>
<ce:given-name>Y.K.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ennis</ce:surname>
<ce:given-name>F.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Influenza virus hemagglutinin-specific cytotoxic T cell response induced by polypeptide produced in Escherichia coli</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>162</sb:volume-nr>
</sb:series>
<sb:date>1985</sb:date>
</sb:issue>
<sb:pages><sb:first-page>663</sb:first-page>
<sb:last-page>674</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib25"><ce:label>25</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Kuwano</ce:surname>
<ce:given-name>K.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Scott</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Young</ce:surname>
<ce:given-name>J.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ennis</ce:surname>
<ce:given-name>F.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Active immunization against virus infections due to antigenic drift by induction of cross-reactive cytotoxic T lymphocytes</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>169</sb:volume-nr>
</sb:series>
<sb:date>1989</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1361</sb:first-page>
<sb:last-page>1371</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib26"><ce:label>26</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Dillon</ce:surname>
<ce:given-name>S.B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Demuth</ce:surname>
<ce:given-name>S.G.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Schneider</ce:surname>
<ce:given-name>M.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Weston</ce:surname>
<ce:given-name>C.B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Jones</ce:surname>
<ce:given-name>C.S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Young</ce:surname>
<ce:given-name>J.F.</ce:given-name>
</sb:author>
<sb:et-al></sb:et-al>
</sb:authors>
<sb:title><sb:maintitle>Induction of protective class I MHC-restricted CTL in mice by a recombinant influenza vaccine in aluminum hydroxide adjuvant</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Vaccine</sb:maintitle>
</sb:title>
<sb:volume-nr>10</sb:volume-nr>
</sb:series>
<sb:date>1992</sb:date>
</sb:issue>
<sb:pages><sb:first-page>309</sb:first-page>
<sb:last-page>318</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib27"><ce:label>27</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Kuwano</ce:surname>
<ce:given-name>K.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Scott</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Young</ce:surname>
<ce:given-name>J.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ennis</ce:surname>
<ce:given-name>F.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>HA<ce:inf loc="post">2</ce:inf>
subunit of influenza HA1 and H2 subtypes induces a protective cross-reactive cytotoxic T lymphocyte response</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>140</sb:volume-nr>
</sb:series>
<sb:date>1988</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1264</sb:first-page>
<sb:last-page>1268</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib28"><ce:label>28</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Schwartz</ce:surname>
<ce:given-name>R.H.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>The value of synthetic peptides as vaccines for eliciting T-cell immunity</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Curr. Top. Microbiol. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>130</sb:volume-nr>
</sb:series>
<sb:date>1986</sb:date>
</sb:issue>
<sb:pages><sb:first-page>79</sb:first-page>
<sb:last-page>85</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib29"><ce:label>29</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Reneger</ce:surname>
<ce:given-name>K.B.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Influenza virus infections and immunity: A review of human and animal models</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Lab. Animal Science</sb:maintitle>
</sb:title>
<sb:volume-nr>42</sb:volume-nr>
</sb:series>
<sb:date>1992</sb:date>
</sb:issue>
<sb:pages><sb:first-page>222</sb:first-page>
<sb:last-page>232</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib30"><ce:label>30</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Schulman</ce:surname>
<ce:given-name>J.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Kilbourne</ce:surname>
<ce:given-name>E.D.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Correlated studies of a recombinant influenza virus vaccine. II Definition of antigenicity in experimental animals</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Infect. Dis.</sb:maintitle>
</sb:title>
<sb:volume-nr>124</sb:volume-nr>
</sb:series>
<sb:date>1971</sb:date>
</sb:issue>
<sb:pages><sb:first-page>463</sb:first-page>
<sb:last-page>472</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib31"><ce:label>31</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Berendt</ce:surname>
<ce:given-name>R.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Scott</ce:surname>
<ce:given-name>G.H.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Evaluation of commercially prepared vaccines for experimentally induced type A/New Jersey/8/76 influenza virus infections in mice and squirrel monkeys</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Infect. Dis.</sb:maintitle>
</sb:title>
<sb:volume-nr>136</sb:volume-nr>
</sb:series>
<sb:date>1977</sb:date>
</sb:issue>
<sb:pages><sb:first-page>712</sb:first-page>
<sb:last-page>717</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib32"><ce:label>32</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>McLaren</ce:surname>
<ce:given-name>C.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Verbonitz</ce:surname>
<ce:given-name>M.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Daniel</ce:surname>
<ce:given-name>S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Grubbs</ce:surname>
<ce:given-name>G.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ennis</ce:surname>
<ce:given-name>F.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Effect of priming infection on serologic response to whole and subunit influenza virus vaccines in animals</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Infect. Dis.</sb:maintitle>
</sb:title>
<sb:volume-nr>136</sb:volume-nr>
</sb:series>
<sb:date>1977</sb:date>
</sb:issue>
<sb:pages><sb:first-page>706</sb:first-page>
<sb:last-page>711</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib33"><ce:label>33</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Mbawuike</ce:surname>
<ce:given-name>I.N.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Wyde</ce:surname>
<ce:given-name>P.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Anderson</ce:surname>
<ce:given-name>P.M.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Enhancement of the protective efficacy of inactivated influenza A virus vaccine in aged mice by IL-2 liposomes</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Vaccine</sb:maintitle>
</sb:title>
<sb:volume-nr>8</sb:volume-nr>
</sb:series>
<sb:date>1990</sb:date>
</sb:issue>
<sb:pages><sb:first-page>347</sb:first-page>
<sb:last-page>352</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib34"><ce:label>34</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wilson</ce:surname>
<ce:given-name>S.Z.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Knight</ce:surname>
<ce:given-name>V.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Wyde</ce:surname>
<ce:given-name>P.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Drake</ce:surname>
<ce:given-name>S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Couch</ce:surname>
<ce:given-name>R.B.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Amantadine and ribavirin aerosol treatment of influenza A and B infection in mice</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Antimicrob. Agents Chemother.</sb:maintitle>
</sb:title>
<sb:volume-nr>17</sb:volume-nr>
</sb:series>
<sb:date>1980</sb:date>
</sb:issue>
<sb:pages><sb:first-page>642</sb:first-page>
<sb:last-page>648</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib35"><ce:label>35</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wyde</ce:surname>
<ce:given-name>P.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Wilson</ce:surname>
<ce:given-name>M.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Cate</ce:surname>
<ce:given-name>T.R.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Interferon production by leukocytes infiltrating the lungs of mice during primary influenza virus infection</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Infect. Immun.</sb:maintitle>
</sb:title>
<sb:volume-nr>38</sb:volume-nr>
</sb:series>
<sb:date>1982</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1249</sb:first-page>
<sb:last-page>1255</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib36"><ce:label>36</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wilson</ce:surname>
<ce:given-name>S.Z.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Gilbert</ce:surname>
<ce:given-name>B.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Quarles</ce:surname>
<ce:given-name>J.M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Knight</ce:surname>
<ce:given-name>V.</ce:given-name>
</sb:author>
<sb:author><ce:surname>McClung</ce:surname>
<ce:given-name>H.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Moore</ce:surname>
<ce:given-name>R.V.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Couch</ce:surname>
<ce:given-name>R.B.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Treatment of influenza A(H1N1) virus infection with ribavirin aerosol</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Antimicrob. Agents Chemother.</sb:maintitle>
</sb:title>
<sb:volume-nr>26</sb:volume-nr>
</sb:series>
<sb:date>1984</sb:date>
</sb:issue>
<sb:pages><sb:first-page>200</sb:first-page>
<sb:last-page>203</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib37"><ce:label>37</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Frank</ce:surname>
<ce:given-name>A.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Puck</ce:surname>
<ce:given-name>J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Hughes</ce:surname>
<ce:given-name>B.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Cate</ce:surname>
<ce:given-name>T.R.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Microneutralization test for influenza A and B parainfluenza 1 and 2 viruses that uses continuous cell lines and fresh serum enhancement</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Clin. Microbiol.</sb:maintitle>
</sb:title>
<sb:volume-nr>12</sb:volume-nr>
</sb:series>
<sb:date>1980</sb:date>
</sb:issue>
<sb:pages><sb:first-page>426</sb:first-page>
<sb:last-page>432</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib38"><ce:label>38</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lewis</ce:surname>
<ce:given-name>D.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Barron</ce:surname>
<ce:given-name>K.S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Miller</ce:surname>
<ce:given-name>G.P.G.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Rich</ce:surname>
<ce:given-name>R.R.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Multiparameter analysis of human lymphocyte subpopulations using flow cytometry</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Surv. Synth. Pathol. Res.</sb:maintitle>
</sb:title>
<sb:volume-nr>4</sb:volume-nr>
</sb:series>
<sb:date>1985</sb:date>
</sb:issue>
<sb:pages><sb:first-page>237</sb:first-page>
<sb:last-page>247</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib39"><ce:label>39</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Zinkernagel</ce:surname>
<ce:given-name>R.M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Doherty</ce:surname>
<ce:given-name>P.C.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>MHC-restricted cytotoxic T cells</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Adv. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>27</sb:volume-nr>
</sb:series>
<sb:date>1979</sb:date>
</sb:issue>
<sb:pages><sb:first-page>51</sb:first-page>
<sb:last-page>106</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib40"><ce:label>40</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lin</ce:surname>
<ce:given-name>Y.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Askonas</ce:surname>
<ce:given-name>B.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Biological properties of an influenza A virus-specific killer T cell clone. Inhibition of virus replication and induction of delayed-type hypersensitivity reactions</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>154</sb:volume-nr>
</sb:series>
<sb:date>1981</sb:date>
</sb:issue>
<sb:pages><sb:first-page>225</sb:first-page>
<sb:last-page>234</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib41"><ce:label>41</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wraith</ce:surname>
<ce:given-name>D.C.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Vessey</ce:surname>
<ce:given-name>A.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Askonas</ce:surname>
<ce:given-name>B.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Purified influenza nucleoprotein protects mice from lethal infection</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Gen. Virol.</sb:maintitle>
</sb:title>
<sb:volume-nr>68</sb:volume-nr>
</sb:series>
<sb:date>1987</sb:date>
</sb:issue>
<sb:pages><sb:first-page>433</sb:first-page>
<sb:last-page>440</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib42"><ce:label>42</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>T.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Lukacher</ce:surname>
<ce:given-name>A.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Sweetser</ce:surname>
<ce:given-name>M.T.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>V.L.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cytolytic T lymphocyte clones in antiviral immunity; effector function <ce:italic>in vivo</ce:italic>
and mechanism of action</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Podack</ce:surname>
<ce:given-name>E.R.</ce:given-name>
</sb:editor>
</sb:editors>
<sb:title><sb:maintitle>Cytolytic Lymphocytes and Complement: Effectors of the Immune System. Vol. 2</sb:maintitle>
</sb:title>
<sb:date>1988</sb:date>
<sb:publisher><sb:name>CRC Press</sb:name>
<sb:location>Boca Raton, FL</sb:location>
</sb:publisher>
</sb:edited-book>
<sb:pages><sb:first-page>161</sb:first-page>
<sb:last-page>172</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib43"><ce:label>43</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>McMichael</ce:surname>
<ce:given-name>A.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Askonas</ce:surname>
<ce:given-name>B.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Webster</ce:surname>
<ce:given-name>R.G.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Laver</ce:surname>
<ce:given-name>W.G.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Vaccination against influenza: B cell or T cell immunity?</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Immunol. Today</sb:maintitle>
</sb:title>
<sb:volume-nr>3</sb:volume-nr>
</sb:series>
<sb:date>1982</sb:date>
</sb:issue>
<sb:pages><sb:first-page>256</sb:first-page>
<sb:last-page>260</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib44"><ce:label>44</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Fries</ce:surname>
<ce:given-name>L.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Dillon</ce:surname>
<ce:given-name>S.B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Hildreth</ce:surname>
<ce:given-name>J.E.K.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Karron</ce:surname>
<ce:given-name>R.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Funkhouser</ce:surname>
<ce:given-name>A.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Friedman</ce:surname>
<ce:given-name>C.J.</ce:given-name>
</sb:author>
<sb:et-al></sb:et-al>
</sb:authors>
<sb:title><sb:maintitle>Safety and immunogenicity of a recombinant protein influenza A vaccine in adult human volunteers and protective efficacy against wild-type H<ce:inf loc="post">1</ce:inf>
N<ce:inf loc="post">1</ce:inf>
virus challenge</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Infect. Dis.</sb:maintitle>
</sb:title>
<sb:volume-nr>167</sb:volume-nr>
</sb:series>
<sb:date>1993</sb:date>
</sb:issue>
<sb:pages><sb:first-page>593</sb:first-page>
<sb:last-page>601</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib45"><ce:label>45</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lightman</ce:surname>
<ce:given-name>S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Cobbold</ce:surname>
<ce:given-name>S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Waldmann</ce:surname>
<ce:given-name>H.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Askonas</ce:surname>
<ce:given-name>B.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Do L3T4+ T cells act as effector cells in protection against influenza virus infection?</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Immunology</sb:maintitle>
</sb:title>
<sb:volume-nr>62</sb:volume-nr>
</sb:series>
<sb:date>1987</sb:date>
</sb:issue>
<sb:pages><sb:first-page>139</sb:first-page>
<sb:last-page>144</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib46"><ce:label>46</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Allan</ce:surname>
<ce:given-name>W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Tabi</ce:surname>
<ce:given-name>Z.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Cleary</ce:surname>
<ce:given-name>A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Doherty</ce:surname>
<ce:given-name>P.C.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cellular events in the lymph node and lung of mice with influenza</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>144</sb:volume-nr>
</sb:series>
<sb:date>1990</sb:date>
</sb:issue>
<sb:pages><sb:first-page>3980</sb:first-page>
<sb:last-page>3986</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib47"><ce:label>47</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Eichelberger</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Allen</ce:surname>
<ce:given-name>W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Zijlstra</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Jaenisch</ce:surname>
<ce:given-name>R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Doherty</ce:surname>
<ce:given-name>P.C.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Clearance of influenza virus respiratory infection in mice lacking Class I major histocompatibility complex-restricted CD8+ T cells</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>174</sb:volume-nr>
</sb:series>
<sb:date>1991</sb:date>
</sb:issue>
<sb:pages><sb:first-page>875</sb:first-page>
<sb:last-page>880</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib48"><ce:label>48</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Scherle</ce:surname>
<ce:given-name>P.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Palladino</ce:surname>
<ce:given-name>G.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Gerhard</ce:surname>
<ce:given-name>W.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Mice can recover from pulmonary influenza virus infection in the absence of Class I-restricted cytotoxic T cells</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>148</sb:volume-nr>
</sb:series>
<sb:date>1992</sb:date>
</sb:issue>
<sb:pages><sb:first-page>212</sb:first-page>
<sb:last-page>217</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib49"><ce:label>49</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Bender</ce:surname>
<ce:given-name>B.S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Coghan</ce:surname>
<ce:given-name>T.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Zhang</ce:surname>
<ce:given-name>L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Small</ce:surname>
<ce:given-name>P.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Transgenic mice lacking class I major histocompatibility complex-restricted T cells have delayed viral clearance and increased mortality after influenza virus challenge</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>175</sb:volume-nr>
</sb:series>
<sb:date>1992</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1143</sb:first-page>
<sb:last-page>1145</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib50"><ce:label>50</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Aichele</ce:surname>
<ce:given-name>P.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Hengartner</ce:surname>
<ce:given-name>H.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Zinkernagel</ce:surname>
<ce:given-name>R.M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Schulz</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Antiviral cytotoxic T cell response induced by <ce:italic>in vivo</ce:italic>
priming with a free synthetic peptide</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Med.</sb:maintitle>
</sb:title>
<sb:volume-nr>171</sb:volume-nr>
</sb:series>
<sb:date>1990</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1815</sb:first-page>
<sb:last-page>1820</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib51"><ce:label>51</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Jones</ce:surname>
<ce:given-name>P.D.</ce:given-name>
</sb:author>
<sb:author><ce:surname>His</ce:surname>
<ce:given-name>R.T.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Morein</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Lougren</ce:surname>
<ce:given-name>K.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ada</ce:surname>
<ce:given-name>G.L.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cellular immune responses in the immune lung to local immunization with influenza A virus glycoproteins in micelles and immunostimulatory complexes (iscoms)</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Scand. J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>27</sb:volume-nr>
</sb:series>
<sb:date>1988</sb:date>
</sb:issue>
<sb:pages><sb:first-page>645</sb:first-page>
<sb:last-page>652</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib52"><ce:label>52</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Takahashi</ce:surname>
<ce:given-name>H.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Takeshita</ce:surname>
<ce:given-name>T.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Morein</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Putney</ce:surname>
<ce:given-name>S.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Germain</ce:surname>
<ce:given-name>R.N.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Berzofsky</ce:surname>
<ce:given-name>J.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Induction of CD8<ce:sup loc="post">+</ce:sup>
cytotoxic T cells by immunization with purified HIVA envelope protein in ISCOMs</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle>
</sb:title>
<sb:volume-nr>344</sb:volume-nr>
</sb:series>
<sb:date>1990</sb:date>
</sb:issue>
<sb:pages><sb:first-page>873</sb:first-page>
<sb:last-page>875</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib53"><ce:label>53</ce:label>
<sb:reference><sb:comment>(Abstract no. 2912)</sb:comment>
<sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Mbawuike</ce:surname>
<ce:given-name>I.N.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Galarza</ce:surname>
<ce:given-name>J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Summers</ce:surname>
<ce:given-name>D.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Couch</ce:surname>
<ce:given-name>R.B.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Protection of outbred mice against influenza A/H3N2 and A/H1N1 viruses after immunization with baculovirus-expressed influenza A/Udorn nucleoprotein</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>FASEB J.</sb:maintitle>
</sb:title>
<sb:volume-nr>6</sb:volume-nr>
</sb:series>
<sb:date>1992</sb:date>
</sb:issue>
<sb:pages><sb:first-page>A1438</sb:first-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib54"><ce:label>54</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Randall</ce:surname>
<ce:given-name>R.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Young</ce:surname>
<ce:given-name>D.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Southern</ce:surname>
<ce:given-name>J.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Immunization with solid matrix-antibody-antigen complexes containing surface or internal virus structural proteins protects mice from infection with the paramixovirus, simian virus 5</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Gen. Virol.</sb:maintitle>
</sb:title>
<sb:volume-nr>69</sb:volume-nr>
</sb:series>
<sb:date>1988</sb:date>
</sb:issue>
<sb:pages><sb:first-page>2517</sb:first-page>
<sb:last-page>2526</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib55"><ce:label>55</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Fu</ce:surname>
<ce:given-name>Z.F.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Dietzschold</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Schumacher</ce:surname>
<ce:given-name>C.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Wunner</ce:surname>
<ce:given-name>W.H.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ertl</ce:surname>
<ce:given-name>H.C.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Koprowski</ce:surname>
<ce:given-name>H.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Rabies virus nucleoprotein expressed in and purified from insect cells is efficacious as a vaccine</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:edited-book><sb:book-series><sb:series><sb:title><sb:maintitle>Proc. Natl Acad. Sci. USA</sb:maintitle>
</sb:title>
<sb:volume-nr>88</sb:volume-nr>
</sb:series>
</sb:book-series>
<sb:date>1991</sb:date>
</sb:edited-book>
<sb:pages><sb:first-page>2001</sb:first-page>
<sb:last-page>2005</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib56"><ce:label>56</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Sumner</ce:surname>
<ce:given-name>J.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Fekadu</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Shaddock</ce:surname>
<ce:given-name>J.H.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Esposito</ce:surname>
<ce:given-name>J.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Bellini</ce:surname>
<ce:given-name>W.J.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Protection of mice with vaccinia virus recombinants that express the rabies nucleoprotein</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Virology</sb:maintitle>
</sb:title>
<sb:volume-nr>183</sb:volume-nr>
</sb:series>
<sb:date>1991</sb:date>
</sb:issue>
<sb:pages><sb:first-page>703</sb:first-page>
<sb:last-page>710</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib57"><ce:label>57</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Brinckmann</ce:surname>
<ce:given-name>U.G.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Bankamp</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Reich</ce:surname>
<ce:given-name>A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Meulen</ce:surname>
<ce:given-name>V.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Liebert</ce:surname>
<ce:given-name>U.G.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Efficacy of individual measles virus structural proteins in the protection of rats from measles encephalitis</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Gen. Virol.</sb:maintitle>
</sb:title>
<sb:volume-nr>72</sb:volume-nr>
</sb:series>
<sb:date>1991</sb:date>
</sb:issue>
<sb:pages><sb:first-page>2491</sb:first-page>
<sb:last-page>2500</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib58"><ce:label>58</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Kast</ce:surname>
<ce:given-name>W.M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Roux</ce:surname>
<ce:given-name>L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Curren</ce:surname>
<ce:given-name>J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Blom</ce:surname>
<ce:given-name>H.J.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Voordouw</ce:surname>
<ce:given-name>A.C.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Meloen</ce:surname>
<ce:given-name>R.H.</ce:given-name>
</sb:author>
<sb:et-al></sb:et-al>
</sb:authors>
<sb:title><sb:maintitle>Protection against lethal Sendai virus infection by in vivo priming of virus-specific cytotoxic T lymphocytes with a free synthetic peptide</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:edited-book><sb:book-series><sb:series><sb:title><sb:maintitle>Proc. Natl Acad. Sci. U S A</sb:maintitle>
</sb:title>
<sb:volume-nr>88</sb:volume-nr>
</sb:series>
</sb:book-series>
<sb:date>1991</sb:date>
</sb:edited-book>
<sb:pages><sb:first-page>2283</sb:first-page>
<sb:last-page>2287</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib59"><ce:label>59</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Connors</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Collins</ce:surname>
<ce:given-name>P.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Firestone</ce:surname>
<ce:given-name>C.Y.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Murphy</ce:surname>
<ce:given-name>B.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Respiratory syncytial virus (RSV) F.G.M2 (22K), and N proteins each induce resistance to RSV challenge, but resistance induced by M2 and N proteins is relatively short-lived</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Virol.</sb:maintitle>
</sb:title>
<sb:volume-nr>65</sb:volume-nr>
</sb:series>
<sb:date>1991</sb:date>
</sb:issue>
<sb:pages><sb:first-page>1634</sb:first-page>
<sb:last-page>1637</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib60"><ce:label>60</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Morrison</ce:surname>
<ce:given-name>L.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>V.L.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Braciale</ce:surname>
<ce:given-name>T.J.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Antigen form influences induction and frequency of influenza-specific class I and class II MHC-restricted cytolytic T lymphocytes</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle>
</sb:title>
<sb:volume-nr>141</sb:volume-nr>
</sb:series>
<sb:date>1988</sb:date>
</sb:issue>
<sb:pages><sb:first-page>363</sb:first-page>
<sb:last-page>368</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib61"><ce:label>61</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Kuwano</ce:surname>
<ce:given-name>K.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Tanura</ce:surname>
<ce:given-name>M.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Ennis</ce:surname>
<ce:given-name>F.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Cross-reactive protection against influenza A virus infections by an NS<ce:inf loc="post">1</ce:inf>
-specific CTL clone</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Virology</sb:maintitle>
</sb:title>
<sb:volume-nr>178</sb:volume-nr>
</sb:series>
<sb:date>1990</sb:date>
</sb:issue>
<sb:pages><sb:first-page>174</sb:first-page>
<sb:last-page>179</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib62"><ce:label>62</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wathen</ce:surname>
<ce:given-name>M.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Brideau</ce:surname>
<ce:given-name>R.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Thomsen</ce:surname>
<ce:given-name>D.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Murphy</ce:surname>
<ce:given-name>B.R.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Characterization of a novel human respiratory syncytial virus chimeric FG glycoprotein expressed using a baculovirus vector</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Gen. Virol.</sb:maintitle>
</sb:title>
<sb:volume-nr>70</sb:volume-nr>
</sb:series>
<sb:date>1989</sb:date>
</sb:issue>
<sb:pages><sb:first-page>2632</sb:first-page>
<sb:last-page>2635</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib63"><ce:label>63</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Brideau</ce:surname>
<ce:given-name>R.J.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Walters</ce:surname>
<ce:given-name>R.R.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Stier</ce:surname>
<ce:given-name>M.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Wathen</ce:surname>
<ce:given-name>M.W.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Protection of cotton rats against human respiratory syncytial virus by vaccination with a novel chimeric FG glycoprotein</sb:maintitle>
</sb:title>
</sb:contribution>
<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Gen. Virol.</sb:maintitle>
</sb:title>
<sb:volume-nr>70</sb:volume-nr>
</sb:series>
<sb:date>1989</sb:date>
</sb:issue>
<sb:pages><sb:first-page>2637</sb:first-page>
<sb:last-page>2644</sb:last-page>
</sb:pages>
</sb:host>
</sb:reference>
</ce:bib-reference>
<ce:bib-reference id="bib64"><ce:label>64</ce:label>
<sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Ievely</ce:surname>
<ce:given-name>M.E.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Bannow</ce:surname>
<ce:given-name>C.A.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Smith</ce:surname>
<ce:given-name>C.W.</ce:given-name>
</sb:author>
<sb:author><ce:surname>Nicholas</ce:surname>
<ce:given-name>J.A.</ce:given-name>
</sb:author>
</sb:authors>
<sb:title><sb:maintitle>Immunodominant T-cell epitope on the F protein of respiratory syncytial virus recognized by human lymphocytes</sb:maintitle>
</sb:title>
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<sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Virol</sb:maintitle>
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<sb:volume-nr>65</sb:volume-nr>
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<sb:date>1991</sb:date>
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<sb:pages><sb:first-page>3789</sb:first-page>
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<mods version="3.6"><titleInfo lang="en"><title>Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS1/HA2 influenza virus protein</title>
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<titleInfo type="alternative" lang="en" contentType="CDATA"><title>Influenza A subtype cross-protection after immunization of outbred mice with a purified chimeric NS</title>
</titleInfo>
<name type="personal"><namePart type="given">Innocent N.</namePart>
<namePart type="family">Mbawuike</namePart>
<affiliation>Acute Viral Respiratory Disease Unit, Influenza Research Center, Department of Microbiology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030-3498, USA</affiliation>
<affiliation>To whom all correspondence should be addressed</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Susan B.</namePart>
<namePart type="family">Dillon</namePart>
<affiliation>Department of Molecular Virology and Host Defense SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Sandra G.</namePart>
<namePart type="family">Demuth</namePart>
<affiliation>Department of Molecular Virology and Host Defense SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Christopher S.</namePart>
<namePart type="family">Jones</namePart>
<affiliation>Department of Molecular Virology and Host Defense SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Thomas R.</namePart>
<namePart type="family">Cate</namePart>
<affiliation>Acute Viral Respiratory Disease Unit, Influenza Research Center, Department of Microbiology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030-3498, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Robert B.</namePart>
<namePart type="family">Couch</namePart>
<affiliation>Acute Viral Respiratory Disease Unit, Influenza Research Center, Department of Microbiology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030-3498, USA</affiliation>
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<dateIssued encoding="w3cdtf">1994</dateIssued>
<dateModified encoding="w3cdtf">1994-03-29</dateModified>
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<abstract lang="en">Influenza A/PR/8/34-derived chimeric (D) protein (SK&F 106160) composed of the first 81 amino acids (aa) of NS1 fused to the conserved 157 C-terminal aa of HA2 (NS11-81-HA 265-222) was previously shown to induce H-2d-restricted protective cytotoxic T-lymphocyte (CTL) immunity in inbred mice. However, D protein, like other small peptides, exhibited haplotype dependence and was not immunogenic in H-2b and H-2K mice. A potential use of this antigen in humans and the role of T cells in any protection were evaluated in outbred Swiss and inbred CBF6F1 (H-2d/b) mice. Mice immunized with D protein and challenged by small-particle aerosol with a lethal dose of influenza virus were significantly protected against mortality from influenza A/HINI and A/H2N2 (p < 0.05- < 0.0000001), but not from A/H3N2 and influenza B viruses when compared with control mice. D protein did not induce serum virus-neutralizing antibody but caused virus to be cleared faster in immunized mice. Protection was long-lasting. In vivo depletion of either Lyt2 (CD8+) or L3T4 (CD4+) T cells with monoclonal antibodies led to abrogation of in vitro-generated CTL activity in CF6F1 mice and significant reduction in the protective efficacy of D protein against virus challenge in both Swiss and CF6F1 mice. These results suggest that protection was mediated by CD8+ and/or CD4+ cells and not antibody. Thus D protein, via a conserved sequence on the HA2 polypeptide, has the potential to induce partially cross-reactive CTL that may protect against influenza virus disease in humans.</abstract>
<subject lang="en"><genre>Keywords</genre>
<topic>Chimeric influenza protein</topic>
<topic>influenza A subtype</topic>
<topic>cross-protection</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Vaccine</title>
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<titleInfo type="abbreviated"><title>JVAC</title>
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<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1994</dateIssued>
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<identifier type="ISSN">0264-410X</identifier>
<identifier type="PII">S0264-410X(94)X8024-7</identifier>
<part><date>1994</date>
<detail type="volume"><number>12</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>14</number>
<caption>no.</caption>
</detail>
<extent unit="issue-pages"><start>1251</start>
<end>1360</end>
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<extent unit="pages"><start>1340</start>
<end>1348</end>
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<identifier type="DOI">10.1016/S0264-410X(94)80063-6</identifier>
<identifier type="PII">S0264-410X(94)80063-6</identifier>
<identifier type="ArticleID">94800636</identifier>
<accessCondition type="use and reproduction" contentType="copyright">©1994 Butterworth-Heinemann Ltd</accessCondition>
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