Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans
Identifieur interne : 000529 ( Istex/Corpus ); précédent : 000528; suivant : 000530Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans
Auteurs : Kuniaki Nerome ; Yasuaki Hiromoto ; Stephen E. Lindstrom ; Shigeo SugitaSource :
- International congress series [ 0531-5131 ] ; 2001.
English descriptors
- Teeft :
- Acid changes, Amino, Amino acid, Amino acid changes, Amino acid residues, Amino acid sequences, Amino acid substitutions, Amino acids, Avian, Avian influenza, Avian species, Brain clones, Causative viruses, Clone, Elsevier science, Evolutionary divergence, Experimental infections, Fatal infection, Gene constellations, Genetic basis, Hong kong, Host range, Human influenza, Human influenza viruses, Human strains, Human virus, Human viruses, Influenza, Influenza virus, Influenza viruses, Internal genes, International congress series, Intracerebral routes, Lineage, Mdck, Mdck cells, Minor lineage, Minor lineages, Mouse, Mouse brain, Negative control, Nerome, Neurovirulence, Nucleotide, Nucleotide sequences, Parental, Parental virus, Parental viruses, Particular interest, Pathogenic, Pathogenic strains, Pathogenicity, Phylogenetic, Phylogenetic analysis, Phylogenetic trees, Present study, Southern china, Variant groups, Virol, Virology, Virulence, Virus, Virus titers.
Abstract
Abstract: Nucleotide and amino acid sequence homologies of the entire RNA segments of six H5N1 human influenza viruses were divided into two or three variant groups. This result was compatible with that of phylogenetic analysis, which showed that trees of the eight genes were composed of two or three minor branch clusters. The PB2, PA, NP and M proteins of human H5N1 viruses were further characterized by the presence of previously reported amino acid sequences peculiar to those of human strains. The virulence of A/Hong Kong/483/97 (HK483) in mice exhibited a sharp contrast to that of A/Hong Kong/156/97 (HK156). Virulence of the former even remained after constant repeated passage in eggs, while the latter rapidly decreased in virulence for mice after only a few passages in eggs. It became apparent that Madin–Darby canine kidney (MDCK) cell-grown virus (HK156-CK) and its clones derived from mouse brain produced a fatal infection in mice through intranasal (i.n.) or intracerebral (i.c.) routes. Conversely, the pathogenicity shown by the egg-derived parental virus (HK156-E3) and its clones were considerably lower in mice after i.n. or i.c. infection. A series of experimental infections revealed that the marked differences in neurovirulence among viruses could be attributed to whether or not viruses were transmitted from the lung to the brain. In amino acid sequence comparison of the entire proteins, it was suggested that a total of six amino acid differences in the PB1(residues 456 and 712), PA (residue 631), HA (residue 211), NP (residue 127) and NS1 (residue101) proteins related closely to changes in pathogenicity or neurovirulence of the HK156 virus in mice. As a result, it was evident that less pathogenic and neurovirulent strains appeared through adaptation and selection of variants during passage in eggs.
Url:
DOI: 10.1016/S0531-5131(01)00402-2
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ISTEX:C08051B7FFEA25D538719895C40B3CE7CE76C9BBLe document en format XML
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Lindstrom</name><affiliation><mods:affiliation>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</mods:affiliation></affiliation></author><author><name sortKey="Sugita, Shigeo" sort="Sugita, Shigeo" uniqKey="Sugita S" first="Shigeo" last="Sugita">Shigeo Sugita</name><affiliation><mods:affiliation>Epizootic Research Station, Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Kokubunji-machi, Shimotuga-gun, Tochigi, Japan</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">International congress series</title><title level="j" type="abbrev">ICS</title><idno type="ISSN">0531-5131</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">1219</biblScope><biblScope unit="supplement">C</biblScope><biblScope unit="page" from="213">213</biblScope><biblScope unit="page" to="223">223</biblScope></imprint><idno type="ISSN">0531-5131</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0531-5131</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acid changes</term><term>Amino</term><term>Amino acid</term><term>Amino acid changes</term><term>Amino acid residues</term><term>Amino acid sequences</term><term>Amino acid substitutions</term><term>Amino acids</term><term>Avian</term><term>Avian influenza</term><term>Avian species</term><term>Brain clones</term><term>Causative viruses</term><term>Clone</term><term>Elsevier science</term><term>Evolutionary divergence</term><term>Experimental infections</term><term>Fatal infection</term><term>Gene constellations</term><term>Genetic basis</term><term>Hong kong</term><term>Host range</term><term>Human influenza</term><term>Human influenza viruses</term><term>Human strains</term><term>Human virus</term><term>Human viruses</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Internal genes</term><term>International congress series</term><term>Intracerebral routes</term><term>Lineage</term><term>Mdck</term><term>Mdck cells</term><term>Minor lineage</term><term>Minor lineages</term><term>Mouse</term><term>Mouse brain</term><term>Negative control</term><term>Nerome</term><term>Neurovirulence</term><term>Nucleotide</term><term>Nucleotide sequences</term><term>Parental</term><term>Parental virus</term><term>Parental viruses</term><term>Particular interest</term><term>Pathogenic</term><term>Pathogenic strains</term><term>Pathogenicity</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Phylogenetic trees</term><term>Present study</term><term>Southern china</term><term>Variant groups</term><term>Virol</term><term>Virology</term><term>Virulence</term><term>Virus</term><term>Virus titers</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Nucleotide and amino acid sequence homologies of the entire RNA segments of six H5N1 human influenza viruses were divided into two or three variant groups. This result was compatible with that of phylogenetic analysis, which showed that trees of the eight genes were composed of two or three minor branch clusters. The PB2, PA, NP and M proteins of human H5N1 viruses were further characterized by the presence of previously reported amino acid sequences peculiar to those of human strains. The virulence of A/Hong Kong/483/97 (HK483) in mice exhibited a sharp contrast to that of A/Hong Kong/156/97 (HK156). Virulence of the former even remained after constant repeated passage in eggs, while the latter rapidly decreased in virulence for mice after only a few passages in eggs. It became apparent that Madin–Darby canine kidney (MDCK) cell-grown virus (HK156-CK) and its clones derived from mouse brain produced a fatal infection in mice through intranasal (i.n.) or intracerebral (i.c.) routes. Conversely, the pathogenicity shown by the egg-derived parental virus (HK156-E3) and its clones were considerably lower in mice after i.n. or i.c. infection. A series of experimental infections revealed that the marked differences in neurovirulence among viruses could be attributed to whether or not viruses were transmitted from the lung to the brain. In amino acid sequence comparison of the entire proteins, it was suggested that a total of six amino acid differences in the PB1(residues 456 and 712), PA (residue 631), HA (residue 211), NP (residue 127) and NS1 (residue101) proteins related closely to changes in pathogenicity or neurovirulence of the HK156 virus in mice. 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Lindstrom</name><affiliations><json:string>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</json:string></affiliations></json:item><json:item><name>Shigeo Sugita</name><affiliations><json:string>Epizootic Research Station, Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Kokubunji-machi, Shimotuga-gun, Tochigi, Japan</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>H5N1 viruses</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Evolution</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Virulence</value></json:item></subject><arkIstex>ark:/67375/6H6-3TGZ0G8S-B</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Nucleotide and amino acid sequence homologies of the entire RNA segments of six H5N1 human influenza viruses were divided into two or three variant groups. This result was compatible with that of phylogenetic analysis, which showed that trees of the eight genes were composed of two or three minor branch clusters. The PB2, PA, NP and M proteins of human H5N1 viruses were further characterized by the presence of previously reported amino acid sequences peculiar to those of human strains. The virulence of A/Hong Kong/483/97 (HK483) in mice exhibited a sharp contrast to that of A/Hong Kong/156/97 (HK156). Virulence of the former even remained after constant repeated passage in eggs, while the latter rapidly decreased in virulence for mice after only a few passages in eggs. It became apparent that Madin–Darby canine kidney (MDCK) cell-grown virus (HK156-CK) and its clones derived from mouse brain produced a fatal infection in mice through intranasal (i.n.) or intracerebral (i.c.) routes. Conversely, the pathogenicity shown by the egg-derived parental virus (HK156-E3) and its clones were considerably lower in mice after i.n. or i.c. infection. A series of experimental infections revealed that the marked differences in neurovirulence among viruses could be attributed to whether or not viruses were transmitted from the lung to the brain. In amino acid sequence comparison of the entire proteins, it was suggested that a total of six amino acid differences in the PB1(residues 456 and 712), PA (residue 631), HA (residue 211), NP (residue 127) and NS1 (residue101) proteins related closely to changes in pathogenicity or neurovirulence of the HK156 virus in mice. As a result, it was evident that less pathogenic and neurovirulent strains appeared through adaptation and selection of variants during passage in eggs.</abstract><qualityIndicators><score>9.039</score><pdfWordCount>4039</pdfWordCount><pdfCharCount>24104</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>544 x 743 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>287</abstractWordCount><abstractCharCount>1839</abstractCharCount><keywordCount>3</keywordCount></qualityIndicators><title>Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans</title><pii><json:string>S0531-5131(01)00402-2</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>International congress series</title><language><json:string>unknown</json:string></language><publicationDate>2001</publicationDate><issn><json:string>0531-5131</json:string></issn><pii><json:string>S0531-5131(00)X0003-9</json:string></pii><volume>1219</volume><pages><first>213</first><last>223</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1-1-1</json:string><json:string>1957</json:string><json:string>1997</json:string><json:string>2001</json:string><json:string>1-1-2</json:string><json:string>1918</json:string><json:string>3-1-1</json:string><json:string>during the 20th century</json:string><json:string>1967</json:string><json:string>7-4-1</json:string></date><geogName></geogName><orgName><json:string>Japan SLC Shizuoka, Japan</json:string><json:string>Japan Abstract Nucleotide and amino acid</json:string><json:string>International Congress Series</json:string><json:string>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Hong Kong</json:string><json:string>Lys Lys</json:string><json:string>Brain Brain</json:string><json:string>Ala Tyr</json:string><json:string>Although</json:string></persName><placeName><json:string>Hong Kong</json:string><json:string>China</json:string><json:string>Tokyo</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>K. Nerome et al.</json:string><json:string>[4]</json:string><json:string>[18]</json:string><json:string>[13,15,20]</json:string><json:string>[13,15]</json:string><json:string>Lu et al. [16]</json:string><json:string>[2,3]</json:string><json:string>[17]</json:string><json:string>[5,6]</json:string><json:string>[1]</json:string><json:string>[10,13]</json:string><json:string>[11,15,16]</json:string><json:string>[15]</json:string><json:string>[7]</json:string><json:string>[19,20]</json:string><json:string>[14]</json:string><json:string>Epizootic Research Station, Equine Research Institute, Japan Racing Association, 1400</json:string><json:string>November and December 1997</json:string><json:string>Zhou et al. [19]</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-3TGZ0G8S-B</json:string></ark><categories><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1016/S0531-5131(01)00402-2</json:string></doi><id>C08051B7FFEA25D538719895C40B3CE7CE76C9BB</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-3TGZ0G8S-B/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-3TGZ0G8S-B/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-3TGZ0G8S-B/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©2001 Elsevier Science B.V.</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>2001</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Fig. 1: Phylogenetic trees of PB2 (a) and NP (b) genes of influenza A viruses including those of Hong Kong H5N1 influenza viruses isolated from humans and chickens. Internal branching probabilities were determined by boostrap analysis using 500 replications and are indicated as percentages at each branch point.</note><note type="content">Table 1: Genomic constellation and amino acids in the internal genes of Hong Kong H5N1 viruses similar to those of avian or human viruses</note><note type="content">Table 2: Growth comparison and virulence of HK156 and its cloned viruses prepared in the study</note><note type="content">Table 3: Comparison of the mortality rate of mouse brain- and chick embryo cell-cloned viruses after i.n. and i.c. infection in mice</note><note type="content">Table 4: Comparative growth profiles of HK156 and its cloned viruses in mouse organs after i.n. or i.c. infectiona</note><note type="content">Table 5: Amino acid changes of HK156 and its cloned viruses in relation to the pathogenicity and neurovirulence</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans</title><author xml:id="author-0000"><persName><forename type="first">Kuniaki</forename><surname>Nerome</surname></persName><note type="correspondence"><p>Corresponding author</p></note><affiliation>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Yasuaki</forename><surname>Hiromoto</surname></persName><affiliation>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Stephen E.</forename><surname>Lindstrom</surname></persName><affiliation>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Shigeo</forename><surname>Sugita</surname></persName><affiliation>Epizootic Research Station, Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Kokubunji-machi, Shimotuga-gun, Tochigi, Japan</affiliation></author><idno type="istex">C08051B7FFEA25D538719895C40B3CE7CE76C9BB</idno><idno type="ark">ark:/67375/6H6-3TGZ0G8S-B</idno><idno type="DOI">10.1016/S0531-5131(01)00402-2</idno><idno type="PII">S0531-5131(01)00402-2</idno></analytic><monogr><title level="j">International congress series</title><title level="j" type="abbrev">ICS</title><idno type="pISSN">0531-5131</idno><idno type="PII">S0531-5131(00)X0003-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">1219</biblScope><biblScope unit="supplement">C</biblScope><biblScope unit="page" from="213">213</biblScope><biblScope unit="page" to="223">223</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Nucleotide and amino acid sequence homologies of the entire RNA segments of six H5N1 human influenza viruses were divided into two or three variant groups. This result was compatible with that of phylogenetic analysis, which showed that trees of the eight genes were composed of two or three minor branch clusters. The PB2, PA, NP and M proteins of human H5N1 viruses were further characterized by the presence of previously reported amino acid sequences peculiar to those of human strains. The virulence of A/Hong Kong/483/97 (HK483) in mice exhibited a sharp contrast to that of A/Hong Kong/156/97 (HK156). Virulence of the former even remained after constant repeated passage in eggs, while the latter rapidly decreased in virulence for mice after only a few passages in eggs. It became apparent that Madin–Darby canine kidney (MDCK) cell-grown virus (HK156-CK) and its clones derived from mouse brain produced a fatal infection in mice through intranasal (i.n.) or intracerebral (i.c.) routes. Conversely, the pathogenicity shown by the egg-derived parental virus (HK156-E3) and its clones were considerably lower in mice after i.n. or i.c. infection. A series of experimental infections revealed that the marked differences in neurovirulence among viruses could be attributed to whether or not viruses were transmitted from the lung to the brain. In amino acid sequence comparison of the entire proteins, it was suggested that a total of six amino acid differences in the PB1(residues 456 and 712), PA (residue 631), HA (residue 211), NP (residue 127) and NS1 (residue101) proteins related closely to changes in pathogenicity or neurovirulence of the HK156 virus in mice. As a result, it was evident that less pathogenic and neurovirulent strains appeared through adaptation and selection of variants during passage in eggs.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>H5N1 viruses</term></item><item><term>Evolution</term></item><item><term>Virulence</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-3TGZ0G8S-B/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>ICS</jid><aid>427</aid><ce:pii>S0531-5131(01)00402-2</ce:pii><ce:doi>10.1016/S0531-5131(01)00402-2</ce:doi><ce:copyright type="full-transfer" year="2001">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans</ce:title><ce:author-group><ce:author><ce:given-name>Kuniaki</ce:given-name><ce:surname>Nerome</ce:surname><ce:cross-ref refid="COR1">*</ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Yasuaki</ce:given-name><ce:surname>Hiromoto</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Stephen E.</ce:given-name><ce:surname>Lindstrom</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Shigeo</ce:given-name><ce:surname>Sugita</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Epizootic Research Station, Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Kokubunji-machi, Shimotuga-gun, Tochigi, Japan</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>*</ce:label><ce:text>Corresponding author</ce:text></ce:correspondence></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Nucleotide and amino acid sequence homologies of the entire RNA segments of six H5N1 human influenza viruses were divided into two or three variant groups. This result was compatible with that of phylogenetic analysis, which showed that trees of the eight genes were composed of two or three minor branch clusters. The PB2, PA, NP and M proteins of human H5N1 viruses were further characterized by the presence of previously reported amino acid sequences peculiar to those of human strains. The virulence of A/Hong Kong/483/97 (HK483) in mice exhibited a sharp contrast to that of A/Hong Kong/156/97 (HK156). Virulence of the former even remained after constant repeated passage in eggs, while the latter rapidly decreased in virulence for mice after only a few passages in eggs. It became apparent that Madin–Darby canine kidney (MDCK) cell-grown virus (HK156-CK) and its clones derived from mouse brain produced a fatal infection in mice through intranasal (i.n.) or intracerebral (i.c.) routes. Conversely, the pathogenicity shown by the egg-derived parental virus (HK156-E3) and its clones were considerably lower in mice after i.n. or i.c. infection. A series of experimental infections revealed that the marked differences in neurovirulence among viruses could be attributed to whether or not viruses were transmitted from the lung to the brain. In amino acid sequence comparison of the entire proteins, it was suggested that a total of six amino acid differences in the PB1(residues 456 and 712), PA (residue 631), HA (residue 211), NP (residue 127) and NS1 (residue101) proteins related closely to changes in pathogenicity or neurovirulence of the HK156 virus in mice. As a result, it was evident that less pathogenic and neurovirulent strains appeared through adaptation and selection of variants during passage in eggs.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>H5N1 viruses</ce:text></ce:keyword><ce:keyword><ce:text>Evolution</ce:text></ce:keyword><ce:keyword><ce:text>Virulence</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Analyses of evolutionary and virulence divergency of Hong Kong H5N1 influenza A viruses isolated from humans</title></titleInfo><name type="personal"><namePart type="given">Kuniaki</namePart><namePart type="family">Nerome</namePart><affiliation>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation><description>Corresponding author</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yasuaki</namePart><namePart type="family">Hiromoto</namePart><affiliation>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephen E.</namePart><namePart type="family">Lindstrom</namePart><affiliation>Laboratory of Respiratory Viruses, Department of Virology I, National Institute of Infectious Diseases, 23-1 Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shigeo</namePart><namePart type="family">Sugita</namePart><affiliation>Epizootic Research Station, Equine Research Institute, Japan Racing Association, 1400-4, Shiba, Kokubunji-machi, Shimotuga-gun, Tochigi, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Nucleotide and amino acid sequence homologies of the entire RNA segments of six H5N1 human influenza viruses were divided into two or three variant groups. This result was compatible with that of phylogenetic analysis, which showed that trees of the eight genes were composed of two or three minor branch clusters. The PB2, PA, NP and M proteins of human H5N1 viruses were further characterized by the presence of previously reported amino acid sequences peculiar to those of human strains. The virulence of A/Hong Kong/483/97 (HK483) in mice exhibited a sharp contrast to that of A/Hong Kong/156/97 (HK156). Virulence of the former even remained after constant repeated passage in eggs, while the latter rapidly decreased in virulence for mice after only a few passages in eggs. It became apparent that Madin–Darby canine kidney (MDCK) cell-grown virus (HK156-CK) and its clones derived from mouse brain produced a fatal infection in mice through intranasal (i.n.) or intracerebral (i.c.) routes. Conversely, the pathogenicity shown by the egg-derived parental virus (HK156-E3) and its clones were considerably lower in mice after i.n. or i.c. infection. A series of experimental infections revealed that the marked differences in neurovirulence among viruses could be attributed to whether or not viruses were transmitted from the lung to the brain. In amino acid sequence comparison of the entire proteins, it was suggested that a total of six amino acid differences in the PB1(residues 456 and 712), PA (residue 631), HA (residue 211), NP (residue 127) and NS1 (residue101) proteins related closely to changes in pathogenicity or neurovirulence of the HK156 virus in mice. As a result, it was evident that less pathogenic and neurovirulent strains appeared through adaptation and selection of variants during passage in eggs.</abstract><note type="content">Fig. 1: Phylogenetic trees of PB2 (a) and NP (b) genes of influenza A viruses including those of Hong Kong H5N1 influenza viruses isolated from humans and chickens. Internal branching probabilities were determined by boostrap analysis using 500 replications and are indicated as percentages at each branch point.</note><note type="content">Table 1: Genomic constellation and amino acids in the internal genes of Hong Kong H5N1 viruses similar to those of avian or human viruses</note><note type="content">Table 2: Growth comparison and virulence of HK156 and its cloned viruses prepared in the study</note><note type="content">Table 3: Comparison of the mortality rate of mouse brain- and chick embryo cell-cloned viruses after i.n. and i.c. infection in mice</note><note type="content">Table 4: Comparative growth profiles of HK156 and its cloned viruses in mouse organs after i.n. or i.c. infectiona</note><note type="content">Table 5: Amino acid changes of HK156 and its cloned viruses in relation to the pathogenicity and neurovirulence</note><subject><genre>Keywords</genre><topic>H5N1 viruses</topic><topic>Evolution</topic><topic>Virulence</topic></subject><relatedItem type="host"><titleInfo><title>International congress series</title></titleInfo><titleInfo type="abbreviated"><title>ICS</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued></originInfo><identifier type="ISSN">0531-5131</identifier><identifier type="PII">S0531-5131(00)X0003-9</identifier><part><date>2001</date><detail type="volume"><number>1219</number><caption>vol.</caption></detail><detail type="supplement"><number>C</number><caption>Suppl.</caption></detail><extent unit="issue-pages"><start>1</start><end>1048</end></extent><extent unit="pages"><start>213</start><end>223</end></extent></part></relatedItem><identifier type="istex">C08051B7FFEA25D538719895C40B3CE7CE76C9BB</identifier><identifier type="ark">ark:/67375/6H6-3TGZ0G8S-B</identifier><identifier type="DOI">10.1016/S0531-5131(01)00402-2</identifier><identifier type="PII">S0531-5131(01)00402-2</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Elsevier Science B.V., ©2001</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-3TGZ0G8S-B/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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