Fine specificity analysis of human influenza-specific cloned cell lines
Identifieur interne : 000319 ( Istex/Corpus ); précédent : 000318; suivant : 000320Fine specificity analysis of human influenza-specific cloned cell lines
Auteurs : Ghislaine Sterkers ; Jean Michon ; Yvette Henin ; Elisabeth Gomard ; Claude Hannoun ; Jean Paul LévySource :
- Cellular Immunology [ 0008-8749 ] ; 1985.
English descriptors
- Teeft :
- Antibody synthesis, Antigenic, Antigenic specificities, Antigenic specificity, Assay, Avian strains, Braciale, Cell line, Cell lines, Clone, Cotc2, Cotc2 cells, Cotc6, Cotc8, Culture medium, Cytolytic, Cytolytic activity, Determinant, Different donors, Different influenza virus strains, Different strains, Drw8 phenotype, Effector cells, Epitope, External components, Fine specificity analysis, Functional properties, Hemagglutinin, Hemagglutinin units, Human strains, Immunol, Infectious viruses, Influenza, Influenza strains, Influenza virus, Influenza viruses, Internal components, Large panel, Matrix protein, Neuraminidase, Peripheral blood, Proliferation assay, Proliferation assays, Proliferative response, Recombinant strain, Same pattern, Specific helper activity, Sterkers, Surface glycoproteins, Talo, Target cells, Triplicate cultures, Various numbers, Virus, Virus strain, Virus strains.
Abstract
Abstract: Influenza-specific human-T-cell clones, proliferating in the presence of virus-infected cells with restriction by class II molecules and displaying class II-restricted CTL activity or specific helper activity in antibody synthesis, have been analyzed for antigenic specificities. All of them were obtained by in vitro stimulation against influenza A/Texas virus. In all cases the virus specificity appeared identical in cytolytic and proliferative responses. Three of the clones were broadly cross-reactive, recognizing all or almost all type A influenza strains. The three remaining clones were subtype specific when tested with human strains and recognized the surface glycoproteins of influenza virus. One of these lines reacted with an epitope of the neuraminidase N2 while the other two recognized the hemagglutinin H3. By using a large panel of mammalian and avian influenza strains, it can be demonstrated that hemagglutinin-specific human T cells can recognize a cross-reacting determinant shared by H3 and H4 subtypes of hemagglutinin which has never been detected with antibodies.
Url:
DOI: 10.1016/0008-8749(85)90263-1
Links to Exploration step
ISTEX:98B82D674AD4B78D1FC8A51F6F4CC87E6E947346Le document en format XML
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All of them were obtained by in vitro stimulation against influenza A/Texas virus. In all cases the virus specificity appeared identical in cytolytic and proliferative responses. Three of the clones were broadly cross-reactive, recognizing all or almost all type A influenza strains. The three remaining clones were subtype specific when tested with human strains and recognized the surface glycoproteins of influenza virus. One of these lines reacted with an epitope of the neuraminidase N2 while the other two recognized the hemagglutinin H3. 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All of them were obtained by in vitro stimulation against influenza A/Texas virus. In all cases the virus specificity appeared identical in cytolytic and proliferative responses. Three of the clones were broadly cross-reactive, recognizing all or almost all type A influenza strains. The three remaining clones were subtype specific when tested with human strains and recognized the surface glycoproteins of influenza virus. One of these lines reacted with an epitope of the neuraminidase N2 while the other two recognized the hemagglutinin H3. By using a large panel of mammalian and avian influenza strains, it can be demonstrated that hemagglutinin-specific human T cells can recognize a cross-reacting determinant shared by H3 and H4 subtypes of hemagglutinin which has never been detected with antibodies.</abstract><qualityIndicators><score>7.546</score><pdfWordCount>3650</pdfWordCount><pdfCharCount>23301</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>468 x 684 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>158</abstractWordCount><abstractCharCount>1100</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Fine specificity analysis of human influenza-specific cloned cell lines</title><pmid><json:string>2411429</json:string></pmid><pii><json:string>0008-8749(85)90263-1</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Cellular Immunology</title><language><json:string>unknown</json:string></language><publicationDate>1985</publicationDate><issn><json:string>0008-8749</json:string></issn><pii><json:string>S0008-8749(00)X0342-5</json:string></pii><volume>94</volume><issue>2</issue><pages><first>394</first><last>405</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1985</json:string></date><geogName></geogName><orgName><json:string>National Influenza Reference Center</json:string><json:string>France/MT</json:string><json:string>World Influenza Center, London</json:string><json:string>France/MJ</json:string><json:string>Academic Press,Inc.</json:string><json:string>WHO Collaborating Center, Munich</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Hong Kong</json:string><json:string>Van Reganmortel</json:string><json:string>V. Hinshaw</json:string><json:string>P. Bachman</json:string><json:string>D. 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sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1985</publicationDate><copyrightDate>1985</copyrightDate><doi><json:string>10.1016/0008-8749(85)90263-1</json:string></doi><id>98B82D674AD4B78D1FC8A51F6F4CC87E6E947346</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-VCSW2VWH-L/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-VCSW2VWH-L/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-VCSW2VWH-L/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Fine specificity analysis of human influenza-specific cloned cell lines</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1985</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Fine specificity analysis of human influenza-specific cloned cell lines</title><author xml:id="author-0000"><persName><forename type="first">Ghislaine</forename><surname>Sterkers</surname></persName><affiliation>To whom correspondence should be sent.</affiliation><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Jean</forename><surname>Michon</surname></persName><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Yvette</forename><surname>Henin</surname></persName><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Elisabeth</forename><surname>Gomard</surname></persName><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Claude</forename><surname>Hannoun</surname></persName><affiliation>Unité d'Ecologie Virale (Centre National de la grippe), Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Jean Paul</forename><surname>Lévy</surname></persName><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation></author><idno type="istex">98B82D674AD4B78D1FC8A51F6F4CC87E6E947346</idno><idno type="ark">ark:/67375/6H6-VCSW2VWH-L</idno><idno type="DOI">10.1016/0008-8749(85)90263-1</idno><idno type="PII">0008-8749(85)90263-1</idno><idno type="article-id">85902631</idno></analytic><monogr><title level="j">Cellular Immunology</title><title level="j" type="abbrev">YCIMM</title><idno type="pISSN">0008-8749</idno><idno type="PII">S0008-8749(00)X0342-5</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985"></date><biblScope unit="volume">94</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="394">394</biblScope><biblScope unit="page" to="405">405</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1985</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Influenza-specific human-T-cell clones, proliferating in the presence of virus-infected cells with restriction by class II molecules and displaying class II-restricted CTL activity or specific helper activity in antibody synthesis, have been analyzed for antigenic specificities. All of them were obtained by in vitro stimulation against influenza A/Texas virus. In all cases the virus specificity appeared identical in cytolytic and proliferative responses. Three of the clones were broadly cross-reactive, recognizing all or almost all type A influenza strains. The three remaining clones were subtype specific when tested with human strains and recognized the surface glycoproteins of influenza virus. One of these lines reacted with an epitope of the neuraminidase N2 while the other two recognized the hemagglutinin H3. By using a large panel of mammalian and avian influenza strains, it can be demonstrated that hemagglutinin-specific human T cells can recognize a cross-reacting determinant shared by H3 and H4 subtypes of hemagglutinin which has never been detected with antibodies.</p></abstract></profileDesc><revisionDesc><change when="1985">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-VCSW2VWH-L/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YCIMM</jid><aid>85902631</aid><ce:pii>0008-8749(85)90263-1</ce:pii><ce:doi>10.1016/0008-8749(85)90263-1</ce:doi><ce:copyright type="unknown" year="1985"></ce:copyright></item-info><head><ce:title>Fine specificity analysis of human influenza-specific cloned cell lines</ce:title><ce:author-group><ce:author><ce:given-name>Ghislaine</ce:given-name><ce:surname>Sterkers</ce:surname><ce:cross-ref refid="COR1"><ce:sup loc="post">1</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup loc="post">∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jean</ce:given-name><ce:surname>Michon</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Yvette</ce:given-name><ce:surname>Henin</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Elisabeth</ce:given-name><ce:surname>Gomard</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Claude</ce:given-name><ce:surname>Hannoun</ce:surname><ce:cross-ref refid="AFF2"><ce:sup loc="post">†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jean Paul</ce:given-name><ce:surname>Lévy</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Unité d'Ecologie Virale (Centre National de la grippe), Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom correspondence should be sent.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="22" month="1" year="1985"></ce:date-received><ce:date-accepted day="16" month="4" year="1985"></ce:date-accepted><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">Influenza-specific human-T-cell clones, proliferating in the presence of virus-infected cells with restriction by class II molecules and displaying class II-restricted CTL activity or specific helper activity in antibody synthesis, have been analyzed for antigenic specificities. All of them were obtained by <ce:italic>in vitro</ce:italic> stimulation against influenza A/Texas virus. In all cases the virus specificity appeared identical in cytolytic and proliferative responses. Three of the clones were broadly cross-reactive, recognizing all or almost all type A influenza strains. The three remaining clones were subtype specific when tested with human strains and recognized the surface glycoproteins of influenza virus. One of these lines reacted with an epitope of the neuraminidase N2 while the other two recognized the hemagglutinin H3. By using a large panel of mammalian and avian influenza strains, it can be demonstrated that hemagglutinin-specific human T cells can recognize a cross-reacting determinant shared by H3 and H4 subtypes of hemagglutinin which has never been detected with antibodies.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Fine specificity analysis of human influenza-specific cloned cell lines</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Fine specificity analysis of human influenza-specific cloned cell lines</title></titleInfo><name type="personal"><namePart type="given">Ghislaine</namePart><namePart type="family">Sterkers</namePart><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation><description>To whom correspondence should be sent.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean</namePart><namePart type="family">Michon</namePart><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yvette</namePart><namePart type="family">Henin</namePart><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Elisabeth</namePart><namePart type="family">Gomard</namePart><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Claude</namePart><namePart type="family">Hannoun</namePart><affiliation>Unité d'Ecologie Virale (Centre National de la grippe), Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean Paul</namePart><namePart type="family">Lévy</namePart><affiliation>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27 rue du Faubourg Saint Jacques, 75014, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued><copyrightDate encoding="w3cdtf">1985</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Influenza-specific human-T-cell clones, proliferating in the presence of virus-infected cells with restriction by class II molecules and displaying class II-restricted CTL activity or specific helper activity in antibody synthesis, have been analyzed for antigenic specificities. All of them were obtained by in vitro stimulation against influenza A/Texas virus. In all cases the virus specificity appeared identical in cytolytic and proliferative responses. Three of the clones were broadly cross-reactive, recognizing all or almost all type A influenza strains. The three remaining clones were subtype specific when tested with human strains and recognized the surface glycoproteins of influenza virus. One of these lines reacted with an epitope of the neuraminidase N2 while the other two recognized the hemagglutinin H3. By using a large panel of mammalian and avian influenza strains, it can be demonstrated that hemagglutinin-specific human T cells can recognize a cross-reacting determinant shared by H3 and H4 subtypes of hemagglutinin which has never been detected with antibodies.</abstract><relatedItem type="host"><titleInfo><title>Cellular Immunology</title></titleInfo><titleInfo type="abbreviated"><title>YCIMM</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued></originInfo><identifier type="ISSN">0008-8749</identifier><identifier type="PII">S0008-8749(00)X0342-5</identifier><part><date>1985</date><detail type="volume"><number>94</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>299</start><end>627</end></extent><extent unit="pages"><start>394</start><end>405</end></extent></part></relatedItem><identifier type="istex">98B82D674AD4B78D1FC8A51F6F4CC87E6E947346</identifier><identifier type="ark">ark:/67375/6H6-VCSW2VWH-L</identifier><identifier type="DOI">10.1016/0008-8749(85)90263-1</identifier><identifier type="PII">0008-8749(85)90263-1</identifier><identifier type="ArticleID">85902631</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-VCSW2VWH-L/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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