Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions
Identifieur interne : 000306 ( Istex/Corpus ); précédent : 000305; suivant : 000307Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions
Auteurs : P. Pothier ; G. A. Denoyel ; S. Ghim ; G. Prudhomme De Saint Maur ; F. FreymuthSource :
- European Journal of Clinical Microbiology [ 0722-2211 ] ; 1986-06-01.
English descriptors
- Teeft :
- Antibody, Antibody titer, Antigenic, Antigenic variation, Ascitic fluid, Cases supernatants, Clinical microbiology, Clinical specimens, Culture medium, Diagnostics pasteur, Epidemic period, Human subtypes, Immunofluorescence, Immunofluorescence assay, Influenza, Influenza virus, Institut pasteur, Internal antigen, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoelonal antibodies, Multicentre study, Nasal smears, Nasopharyngeal, Nasopharyngeal samples, Nasopharyngeal secretions, Nosocomial influenza infections, Polyclonal, Polyclonal antibodies, Rapid detection, Rapid diagnosis, Respiratory agents, Respiratory syncytial virus, Such antibodies, Supernatant, Surface epitope, Surface proteins, Viral, Viral culture, Viral isolation, Virus infections, Virus nucleoprotein.
Abstract
Abstract: Two monoclonal antibodies against influenza A virus were assessed for use as diagnostic reagents in an indirect immunofluorescence assay (IFA) of nasopharyngeal secretions. Monoclonal antibody IA-52, directed at an internal antigen, reacted with all influenza A tested. The high stability of this epitope permitted its use in a rapid IFA test, which gave results comparable to those obtained with polyclonal antibodies and viral isolation. The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H3 subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1,H3N2).
Url:
DOI: 10.1007/BF02017792
Links to Exploration step
ISTEX:ED3F373AC320FC327D8E04170F1622530F4D3EA8Le document en format XML
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Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1,H3N2).</div></front></TEI><istex><corpusName>springer-journals</corpusName><keywords><teeft><json:string>monoclonal</json:string><json:string>monoclonal antibodies</json:string><json:string>monoclonal antibody</json:string><json:string>nasopharyngeal</json:string><json:string>immunofluorescence</json:string><json:string>viral</json:string><json:string>influenza</json:string><json:string>viral isolation</json:string><json:string>nasopharyngeal secretions</json:string><json:string>polyclonal</json:string><json:string>antigenic</json:string><json:string>rapid diagnosis</json:string><json:string>polyclonal antibodies</json:string><json:string>rapid detection</json:string><json:string>clinical microbiology</json:string><json:string>antigenic variation</json:string><json:string>supernatant</json:string><json:string>multicentre study</json:string><json:string>nasopharyngeal samples</json:string><json:string>immunofluorescence assay</json:string><json:string>surface proteins</json:string><json:string>influenza virus</json:string><json:string>antibody</json:string><json:string>monoelonal antibodies</json:string><json:string>antibody titer</json:string><json:string>cases supernatants</json:string><json:string>diagnostics pasteur</json:string><json:string>culture medium</json:string><json:string>ascitic fluid</json:string><json:string>institut pasteur</json:string><json:string>respiratory syncytial virus</json:string><json:string>viral culture</json:string><json:string>clinical specimens</json:string><json:string>nosocomial influenza infections</json:string><json:string>such antibodies</json:string><json:string>human subtypes</json:string><json:string>epidemic period</json:string><json:string>surface epitope</json:string><json:string>nasal smears</json:string><json:string>respiratory agents</json:string><json:string>internal antigen</json:string><json:string>virus infections</json:string><json:string>virus nucleoprotein</json:string></teeft></keywords><author><json:item><name>P. 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The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H3 subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1,H3N2).</abstract><qualityIndicators><refBibsNative>false</refBibsNative><abstractWordCount>106</abstractWordCount><abstractCharCount>750</abstractCharCount><keywordCount>0</keywordCount><score>6.026</score><pdfWordCount>2754</pdfWordCount><pdfCharCount>16973</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>4</pdfPageCount><pdfPageSize>540 x 792 pts</pdfPageSize></qualityIndicators><title>Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions</title><pmid><json:string>3527703</json:string></pmid><genre><json:string>research-article</json:string></genre><host><title>European Journal of Clinical Microbiology</title><language><json:string>unknown</json:string></language><publicationDate>1986</publicationDate><copyrightDate>1986</copyrightDate><issn><json:string>0722-2211</json:string></issn><eissn><json:string>1435-4373</json:string></eissn><journalId><json:string>10096</json:string></journalId><volume>5</volume><issue>3</issue><pages><first>336</first><last>339</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Medical Microbiology</value></json:item><json:item><value>Internal Medicine</value></json:item></subject></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/1BB-60133THC-M</json:string></ark><categories><wos></wos><scienceMetrix></scienceMetrix><scopus><json:string>1 - 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J, Clin. Microbiol.</title><idno type="pISSN">0722-2211</idno><idno type="eISSN">1435-4373</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">28</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1986-06-01"></date><biblScope unit="volume">5</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="336">336</biblScope><biblScope unit="page" to="339">339</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1986</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Two monoclonal antibodies against influenza A virus were assessed for use as diagnostic reagents in an indirect immunofluorescence assay (IFA) of nasopharyngeal secretions. Monoclonal antibody IA-52, directed at an internal antigen, reacted with all influenza A tested. The high stability of this epitope permitted its use in a rapid IFA test, which gave results comparable to those obtained with polyclonal antibodies and viral isolation. The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H3 subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1,H3N2).</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Internal Medicine</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1986-06-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-60133THC-M/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>10096</JournalID><JournalPrintISSN>0722-2211</JournalPrintISSN><JournalElectronicISSN>1435-4373</JournalElectronicISSN><JournalTitle>European Journal of Clinical Microbiology</JournalTitle><JournalAbbreviatedTitle>Eur. 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Microbiol.</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Medical Microbiology</JournalSubject><JournalSubject Type="Secondary">Internal Medicine</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>5</VolumeIDStart><VolumeIDEnd>5</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>3</IssueIDStart><IssueIDEnd>3</IssueIDEnd><IssueArticleCount>28</IssueArticleCount><IssueHistory><CoverDate><Year>1986</Year><Month>6</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Friedr, Vieweg & Sohn Verlagsgesellschaft mbH</CopyrightHolderName><CopyrightYear>1986</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art14"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF02017792</ArticleID><ArticleDOI>10.1007/BF02017792</ArticleDOI><ArticleSequenceNumber>14</ArticleSequenceNumber><ArticleTitle Language="En">Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions</ArticleTitle><ArticleCategory>Articles</ArticleCategory><ArticleFirstPage>336</ArticleFirstPage><ArticleLastPage>339</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>7</Month><Day>15</Day></RegistrationDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Friedr, Vieweg & Sohn Verlagsgesellschaft mbH</CopyrightHolderName><CopyrightYear>1986</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>10096</JournalID><VolumeIDStart>5</VolumeIDStart><VolumeIDEnd>5</VolumeIDEnd><IssueIDStart>3</IssueIDStart><IssueIDEnd>3</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>P.</GivenName><FamilyName>Pothier</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>G.</GivenName><GivenName>A.</GivenName><FamilyName>Denoyel</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>S.</GivenName><FamilyName>Ghim</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>G.</GivenName><GivenName>Prudhomme</GivenName><Particle>de</Particle><FamilyName>Saint Maur</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>F.</GivenName><FamilyName>Freymuth</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Faculté de Médecine</OrgDivision><OrgName>Laboratoire de Bactériologie-Virologie</OrgName><OrgAddress><Street>7 Boulevard Jeanne d'Arc</Street><Postcode>21033</Postcode><City>Dijon Cedex</City><Country>France</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Service des Virus</OrgDivision><OrgName>Institut Pasteur de Lyon</OrgName><OrgAddress><Postcode>69365</Postcode><City>Lyon Cedex 7</City><Country>France</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Laboratoire de Virologie</OrgDivision><OrgName>Hôpital Trousseau</OrgName><OrgAddress><Postcode>75571</Postcode><City>Paris Cedex 12</City><Country>France</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgDivision>Laboratoire de Virologie</OrgDivision><OrgName>Centre Hospitalier Universitaire</OrgName><OrgAddress><Postcode>14033</Postcode><City>Caen Cedex</City><Country>France</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Two monoclonal antibodies against influenza A virus were assessed for use as diagnostic reagents in an indirect immunofluorescence assay (IFA) of nasopharyngeal secretions. Monoclonal antibody IA-52, directed at an internal antigen, reacted with all influenza A tested. The high stability of this epitope permitted its use in a rapid IFA test, which gave results comparable to those obtained with polyclonal antibodies and viral isolation. The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H<Subscript>3</Subscript> subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H<Subscript>1</Subscript>N<Subscript>1</Subscript>,H<Subscript>3</Subscript>N<Subscript>2</Subscript>).</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Use of monoclonal antibodies for rapid detection of influenza a viras in nasopharyngeal secretions</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">P.</namePart><namePart type="family">Pothier</namePart><affiliation>Faculté de Médecine, Laboratoire de Bactériologie-Virologie, 7 Boulevard Jeanne d'Arc, 21033, Dijon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="given">A.</namePart><namePart type="family">Denoyel</namePart><affiliation>Service des Virus, Institut Pasteur de Lyon, 69365, Lyon Cedex 7, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Ghim</namePart><affiliation>Faculté de Médecine, Laboratoire de Bactériologie-Virologie, 7 Boulevard Jeanne d'Arc, 21033, Dijon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="given">Prudhomme</namePart><namePart type="family">de Saint Maur</namePart><affiliation>Laboratoire de Virologie, Hôpital Trousseau, 75571, Paris Cedex 12, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Freymuth</namePart><affiliation>Laboratoire de Virologie, Centre Hospitalier Universitaire, 14033, Caen Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateIssued encoding="w3cdtf">1986-06-01</dateIssued><copyrightDate encoding="w3cdtf">1986</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Two monoclonal antibodies against influenza A virus were assessed for use as diagnostic reagents in an indirect immunofluorescence assay (IFA) of nasopharyngeal secretions. Monoclonal antibody IA-52, directed at an internal antigen, reacted with all influenza A tested. The high stability of this epitope permitted its use in a rapid IFA test, which gave results comparable to those obtained with polyclonal antibodies and viral isolation. The second monoclonal antibody, IA-279 was directed at a surface epitope (hemagglutinin); it reacted with almost all H3 subtype strains. Positive IFA using these monoclonal antibodies permitted rapid preliminary differentiation between the current two major subtypes of influenza A virus (H1N1,H3N2).</abstract><note>Articles</note><relatedItem type="host"><titleInfo><title>European Journal of Clinical Microbiology</title></titleInfo><titleInfo type="abbreviated"><title>Eur. J, Clin. Microbiol.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">1986-06-01</dateIssued><copyrightDate encoding="w3cdtf">1986</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Internal Medicine</topic></subject><identifier type="ISSN">0722-2211</identifier><identifier type="eISSN">1435-4373</identifier><identifier type="JournalID">10096</identifier><identifier type="IssueArticleCount">28</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1986</date><detail type="volume"><number>5</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="pages"><start>336</start><end>339</end></extent></part><recordInfo><recordOrigin>Friedr, Vieweg & Sohn Verlagsgesellschaft mbH, 1986</recordOrigin></recordInfo></relatedItem><identifier type="istex">ED3F373AC320FC327D8E04170F1622530F4D3EA8</identifier><identifier type="ark">ark:/67375/1BB-60133THC-M</identifier><identifier type="DOI">10.1007/BF02017792</identifier><identifier type="ArticleID">BF02017792</identifier><identifier type="ArticleID">Art14</identifier><accessCondition type="use and reproduction" contentType="copyright">Friedr, Vieweg & Sohn Verlagsgesellschaft mbH, 1986</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Friedr, Vieweg & Sohn Verlagsgesellschaft mbH, 1986</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/1BB-60133THC-M/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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