Comparison of biological and physical properties of human and animal A(H1N1) influenza viruses
Identifieur interne : 000191 ( Istex/Corpus ); précédent : 000190; suivant : 000192Comparison of biological and physical properties of human and animal A(H1N1) influenza viruses
Auteurs : B. Fiszon ; C. Hannoun ; A. Garcia-Sastre ; E. Villar ; J. A. CabezasSource :
- Research in Virology [ 0923-2516 ] ; 1989.
English descriptors
- KwdEn :
- Teeft :
- Allantoic fluid, Assay, Avian, Avian species, Avian strains, Avian viruses, Body temperature, Enzyme activity, Hannoun, High temperature, Human porcine, Infectious titres, Infectivity, Influenza, Influenza virus, Influenza virus strains, Influenza viruses, Leibowitz medium, Neuraminidase, Neuraminidase activity, Optimal genome composition, Other hand, Phosphate buffer, Physical properties, Porcine, Residual infectivity, Residual sialidase activity, Sialidase, Sialidase activity, Sialidase activity assays, Swine strains, Titre, Viral, Viral sialidase, Viral sialidases, Virol, Virus, Virus grippal, Wild ducks.
Abstract
Summary: The study of biological properties of influenza virus strains belonging to the same subtype A(H1N1) and closely antigenically related, but isolated from different animal species (man, pig and duck), demonstrated that avian strains were more resistant than those isolated from mammals to high temperature and low pH, as shown by titration of residual infectivity in cell cultures (MDCK) and by sialidase assay.The difference in behaviour could be correlated to biological adaptation of the virus to its host. Avian body temperature is 40°C and influenza virus, in ducks, is enterotropic and therefore capable of passing through the low pH values in the upper digestive tract of the animal.These results do not contradict the hypothesis of a possible filiation between avian and mammalian orthomyxoviruses.
Résumé: L'étude des propriétés biologiques et physiques de souches de virus grippaux appartenant au sérotype A(H1N1) isolées d'espèces différentes (homme, porc ou oiseaux) a permis la mise en évidence d'importantes différences. Les souches d'origine aviaire se révèlent plus résistantes à l'action inactivante de la chaleur et des pH acides ainsi que le démontrent les résultats des titrages de pouvoir infectant et les épreuves d'activité de la sialidase. A 40°C, l'activité de la sialidase des souches aviaires n'est pas altérée, même après 90 min d'exposition, alors que la plupart de celles de l'homme ou du porc sont totalement inactivées en 60 min. De même, l'activité enzymatique des virus aviaires n'est pas totalement abolie après 30 min à pH 3,0 alors que 15 min à pH 5,0 suffisent à inactiver toutes les souches de mammifères.Ces différences de comportement peuvent être mises en rapport avec l'adaptation nécessaire de ces virus à leurs hôtes. La température interne des oiseaux est supérieure à 40°C et, chez eux, le virus grippal a un tropisme entérique, ce qui implique un passage par de segments du tube digestif où le pH est très acide.Ces résultats ne contredisent cependant pas les hypothèses de filiation entre les orthomyxovirus d'oiseaux et ceux de mammifères.
Url:
DOI: 10.1016/S0923-2516(89)80118-9
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ISTEX:F17340AB9BF47A5FB3079275005B31037ADC71F9Le document en format XML
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Avian body temperature is 40°C and influenza virus, in ducks, is enterotropic and therefore capable of passing through the low pH values in the upper digestive tract of the animal.These results do not contradict the hypothesis of a possible filiation between avian and mammalian orthomyxoviruses.</div><div type="abstract" xml:lang="fr">Résumé: L'étude des propriétés biologiques et physiques de souches de virus grippaux appartenant au sérotype A(H1N1) isolées d'espèces différentes (homme, porc ou oiseaux) a permis la mise en évidence d'importantes différences. Les souches d'origine aviaire se révèlent plus résistantes à l'action inactivante de la chaleur et des pH acides ainsi que le démontrent les résultats des titrages de pouvoir infectant et les épreuves d'activité de la sialidase. A 40°C, l'activité de la sialidase des souches aviaires n'est pas altérée, même après 90 min d'exposition, alors que la plupart de celles de l'homme ou du porc sont totalement inactivées en 60 min. De même, l'activité enzymatique des virus aviaires n'est pas totalement abolie après 30 min à pH 3,0 alors que 15 min à pH 5,0 suffisent à inactiver toutes les souches de mammifères.Ces différences de comportement peuvent être mises en rapport avec l'adaptation nécessaire de ces virus à leurs hôtes. La température interne des oiseaux est supérieure à 40°C et, chez eux, le virus grippal a un tropisme entérique, ce qui implique un passage par de segments du tube digestif où le pH est très acide.Ces résultats ne contredisent cependant pas les hypothèses de filiation entre les orthomyxovirus d'oiseaux et ceux de mammifères.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>avian</json:string><json:string>sialidase</json:string><json:string>influenza</json:string><json:string>influenza virus</json:string><json:string>neuraminidase</json:string><json:string>porcine</json:string><json:string>virol</json:string><json:string>influenza virus strains</json:string><json:string>infectivity</json:string><json:string>viral</json:string><json:string>hannoun</json:string><json:string>avian viruses</json:string><json:string>influenza viruses</json:string><json:string>assay</json:string><json:string>virus</json:string><json:string>residual sialidase activity</json:string><json:string>sialidase activity</json:string><json:string>viral sialidase</json:string><json:string>wild ducks</json:string><json:string>phosphate buffer</json:string><json:string>other hand</json:string><json:string>swine strains</json:string><json:string>allantoic fluid</json:string><json:string>physical properties</json:string><json:string>virus grippal</json:string><json:string>sialidase activity assays</json:string><json:string>enzyme activity</json:string><json:string>avian species</json:string><json:string>body temperature</json:string><json:string>leibowitz medium</json:string><json:string>residual infectivity</json:string><json:string>human porcine</json:string><json:string>high temperature</json:string><json:string>infectious titres</json:string><json:string>viral sialidases</json:string><json:string>avian strains</json:string><json:string>optimal genome composition</json:string><json:string>neuraminidase activity</json:string><json:string>titre</json:string></teeft></keywords><subject><json:item><lang><json:string>eng</json:string></lang><value>Orthomyxovirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Influenza virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>A(H1N1) subtype</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Physicochemical criteria</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Birds</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Mammals</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Orthomyxovirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Virus grippal</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Critères physico-chimiques</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Oiseaux</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Mammifères</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Sous-type A(H1N1)</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><qualityIndicators><score>6.676</score><pdfWordCount>3200</pdfWordCount><pdfCharCount>18960</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>468 x 684 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>123</abstractWordCount><abstractCharCount>813</abstractCharCount><keywordCount>12</keywordCount></qualityIndicators><title>Comparison of biological and physical properties of human and animal A(H1N1) influenza viruses</title><pii><json:string>S0923-2516(89)80118-9</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Research in Virology</title><language><json:string>unknown</json:string></language><publicationDate>1989</publicationDate><issn><json:string>0923-2516</json:string></issn><pii><json:string>S0923-2516(89)X8001-3</json:string></pii><volume>140</volume><pages><first>395</first><last>404</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1968</json:string><json:string>1976</json:string><json:string>1930s</json:string><json:string>1989</json:string><json:string>1980</json:string></date><geogName></geogName><orgName><json:string>Spain and France</json:string><json:string>"Integrated Action"</json:string></orgName><orgName_funder><json:string>"Integrated Action"</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Paris Cedex</json:string><json:string>E. 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(1983)</json:string><json:string>Murty and Webster, 1986</json:string><json:string>Golubev et al., 1973</json:string><json:string>Nerome et al., 1984</json:string><json:string>Huang et aL, 1985</json:string><json:string>Mancini et al., 1985</json:string><json:string>H a n n o u n and Devaux, 1983</json:string><json:string>Hinshaw et al., 1984</json:string><json:string>Fiszon, 1988</json:string><json:string>Palese et al., 1974</json:string><json:string>Hannoun, 1977</json:string><json:string>De Jong et al., 1988</json:string><json:string>Rott et al., 1982</json:string><json:string>Breuning and Scholtissek, 1974</json:string><json:string>Webster et al., 1978</json:string><json:string>Warner and O'Brien, 1979</json:string><json:string>Rott and Klenk (1986)</json:string><json:string>Kilbourne, 1987</json:string><json:string>Glathe et al., 1982</json:string><json:string>Warner and O'Brien (1979)</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-5D4RSTW8-1</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>1989</publicationDate><author><json:item><name>B. Fiszon</name><affiliations><json:string>Unité d'Ecologie Virale, Institut Pasteur, 75724 Paris Cedex 15, France</json:string></affiliations></json:item><json:item><name>C. Hannoun</name><affiliations><json:string>Unité d'Ecologie Virale, Institut Pasteur, 75724 Paris Cedex 15, France</json:string><json:string>Corresponding author.</json:string></affiliations></json:item><json:item><name>A. Garcia-Sastre</name><affiliations><json:string>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</json:string></affiliations></json:item><json:item><name>E. Villar</name><affiliations><json:string>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</json:string></affiliations></json:item><json:item><name>J.A. Cabezas</name><affiliations><json:string>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</json:string></affiliations></json:item></author><articleId><json:string>80118</json:string></articleId><arkIstex>ark:/67375/6H6-5D4RSTW8-1</arkIstex><abstract>Summary: The study of biological properties of influenza virus strains belonging to the same subtype A(H1N1) and closely antigenically related, but isolated from different animal species (man, pig and duck), demonstrated that avian strains were more resistant than those isolated from mammals to high temperature and low pH, as shown by titration of residual infectivity in cell cultures (MDCK) and by sialidase assay.The difference in behaviour could be correlated to biological adaptation of the virus to its host. Avian body temperature is 40°C and influenza virus, in ducks, is enterotropic and therefore capable of passing through the low pH values in the upper digestive tract of the animal.These results do not contradict the hypothesis of a possible filiation between avian and mammalian orthomyxoviruses.</abstract><pmid><json:string>2587848</json:string></pmid><serie><title>Proc. nat. Acad. Sci. (Wash.)</title><language><json:string>unknown</json:string></language><volume>75</volume><pages><first>3341</first><last>3345</last></pages></serie><copyrightDate>1989</copyrightDate><doi><json:string>10.1016/S0923-2516(89)80118-9</json:string></doi><id>F17340AB9BF47A5FB3079275005B31037ADC71F9</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5D4RSTW8-1/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5D4RSTW8-1/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-5D4RSTW8-1/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Comparison of biological and physical properties of human and animal A(H1N1) influenza viruses</title><title level="a" type="alt" xml:lang="fr">Comparaison entre les propriétés biologiques et physiques des virus grippaux A(H1N1) humains et animaux</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1989 Elsevier</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1989</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Comparison of biological and physical properties of human and animal A(H1N1) influenza viruses</title><title level="a" type="alt" xml:lang="fr">Comparaison entre les propriétés biologiques et physiques des virus grippaux A(H1N1) humains et animaux</title><author xml:id="author-0000"><persName><forename type="first">B.</forename><surname>Fiszon</surname></persName><affiliation>Unité d'Ecologie Virale, Institut Pasteur, 75724 Paris Cedex 15, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">C.</forename><surname>Hannoun</surname></persName><affiliation>Unité d'Ecologie Virale, Institut Pasteur, 75724 Paris Cedex 15, France</affiliation><affiliation>Corresponding author.</affiliation></author><author xml:id="author-0002"><persName><forename type="first">A.</forename><surname>Garcia-Sastre</surname></persName><affiliation>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</affiliation></author><author xml:id="author-0003"><persName><forename type="first">E.</forename><surname>Villar</surname></persName><affiliation>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</affiliation></author><author xml:id="author-0004"><persName><forename type="first">J.A.</forename><surname>Cabezas</surname></persName><affiliation>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</affiliation></author><idno type="istex">F17340AB9BF47A5FB3079275005B31037ADC71F9</idno><idno type="ark">ark:/67375/6H6-5D4RSTW8-1</idno><idno type="DOI">10.1016/S0923-2516(89)80118-9</idno><idno type="PII">S0923-2516(89)80118-9</idno><idno type="article-id">80118</idno></analytic><monogr><title level="j">Research in Virology</title><title level="j" type="abbrev">X135</title><idno type="pISSN">0923-2516</idno><idno type="PII">S0923-2516(89)X8001-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989"></date><biblScope unit="volume">140</biblScope><biblScope unit="supplement">C</biblScope><biblScope unit="page" from="395">395</biblScope><biblScope unit="page" to="404">404</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1989</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Summary: The study of biological properties of influenza virus strains belonging to the same subtype A(H1N1) and closely antigenically related, but isolated from different animal species (man, pig and duck), demonstrated that avian strains were more resistant than those isolated from mammals to high temperature and low pH, as shown by titration of residual infectivity in cell cultures (MDCK) and by sialidase assay.The difference in behaviour could be correlated to biological adaptation of the virus to its host. Avian body temperature is 40°C and influenza virus, in ducks, is enterotropic and therefore capable of passing through the low pH values in the upper digestive tract of the animal.These results do not contradict the hypothesis of a possible filiation between avian and mammalian orthomyxoviruses.</p></abstract><abstract xml:lang="fr"><p>Résumé: L'étude des propriétés biologiques et physiques de souches de virus grippaux appartenant au sérotype A(H1N1) isolées d'espèces différentes (homme, porc ou oiseaux) a permis la mise en évidence d'importantes différences. Les souches d'origine aviaire se révèlent plus résistantes à l'action inactivante de la chaleur et des pH acides ainsi que le démontrent les résultats des titrages de pouvoir infectant et les épreuves d'activité de la sialidase. A 40°C, l'activité de la sialidase des souches aviaires n'est pas altérée, même après 90 min d'exposition, alors que la plupart de celles de l'homme ou du porc sont totalement inactivées en 60 min. De même, l'activité enzymatique des virus aviaires n'est pas totalement abolie après 30 min à pH 3,0 alors que 15 min à pH 5,0 suffisent à inactiver toutes les souches de mammifères.Ces différences de comportement peuvent être mises en rapport avec l'adaptation nécessaire de ces virus à leurs hôtes. La température interne des oiseaux est supérieure à 40°C et, chez eux, le virus grippal a un tropisme entérique, ce qui implique un passage par de segments du tube digestif où le pH est très acide.Ces résultats ne contredisent cependant pas les hypothèses de filiation entre les orthomyxovirus d'oiseaux et ceux de mammifères.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key-words</head><item><term>Orthomyxovirus</term></item><item><term>Influenza virus</term></item><item><term>A(H1N1) subtype</term></item><item><term>Physicochemical criteria</term></item><item><term>Birds</term></item><item><term>Mammals</term></item></list></keywords></textClass><textClass xml:lang="fr"><keywords scheme="keyword"><list><head>Mots-clés</head><item><term>Orthomyxovirus</term></item><item><term>Virus grippal</term></item><item><term>Critères physico-chimiques</term></item><item><term>Oiseaux</term></item><item><term>Mammifères</term></item><item><term>Sous-type A(H1N1)</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1989">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5D4RSTW8-1/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="fla"><item-info><jid>X135</jid><aid>80118</aid><ce:pii>S0923-2516(89)80118-9</ce:pii><ce:doi>10.1016/S0923-2516(89)80118-9</ce:doi><ce:copyright type="unknown" year="1989">Elsevier</ce:copyright></item-info><head><ce:title>Comparison of biological and physical properties of human and animal A(H1N1) influenza viruses</ce:title><ce:alt-title xml:lang="fr">Comparaison entre les propriétés biologiques et physiques des virus grippaux A(H1N1) humains et animaux</ce:alt-title><ce:author-group><ce:author><ce:given-name>B.</ce:given-name><ce:surname>Fiszon</ce:surname><ce:cross-ref refid="aff1"><ce:sup>(1)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C.</ce:given-name><ce:surname>Hannoun</ce:surname><ce:cross-ref refid="aff1"><ce:sup>(1)</ce:sup></ce:cross-ref><ce:cross-ref refid="cor1"><ce:sup>(*)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Garcia-Sastre</ce:surname><ce:cross-ref refid="aff2"><ce:sup>(2)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Villar</ce:surname><ce:cross-ref refid="aff2"><ce:sup>(2)</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.A.</ce:given-name><ce:surname>Cabezas</ce:surname><ce:cross-ref refid="aff2"><ce:sup>(2)</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:label>(1)</ce:label><ce:textfn>Unité d'Ecologie Virale, Institut Pasteur, 75724 Paris Cedex 15, France</ce:textfn></ce:affiliation><ce:affiliation id="aff2"><ce:label>(2)</ce:label><ce:textfn>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>(*)</ce:label><ce:text>Corresponding author.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="25" month="4" year="1989"></ce:date-received><ce:date-accepted day="31" month="8" year="1989"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Summary</ce:section-title><ce:abstract-sec><ce:simple-para>The study of biological properties of influenza virus strains belonging to the same subtype A(H1N1) and closely antigenically related, but isolated from different animal species (man, pig and duck), demonstrated that avian strains were more resistant than those isolated from mammals to high temperature and low pH, as shown by titration of residual infectivity in cell cultures (MDCK) and by sialidase assay.</ce:simple-para><ce:simple-para>The difference in behaviour could be correlated to biological adaptation of the virus to its host. Avian body temperature is 40°C and influenza virus, in ducks, is enterotropic and therefore capable of passing through the low pH values in the upper digestive tract of the animal.</ce:simple-para><ce:simple-para>These results do not contradict the hypothesis of a possible filiation between avian and mammalian orthomyxoviruses.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:abstract id="ab2" class="author" xml:lang="fr"><ce:section-title>Résumé</ce:section-title><ce:abstract-sec><ce:simple-para>L'étude des propriétés biologiques et physiques de souches de virus grippaux appartenant au sérotype A(H1N1) isolées d'espèces différentes (homme, porc ou oiseaux) a permis la mise en évidence d'importantes différences. Les souches d'origine aviaire se révèlent plus résistantes à l'action inactivante de la chaleur et des pH acides ainsi que le démontrent les résultats des titrages de pouvoir infectant et les épreuves d'activité de la sialidase. A 40°C, l'activité de la sialidase des souches aviaires n'est pas altérée, même après 90 min d'exposition, alors que la plupart de celles de l'homme ou du porc sont totalement inactivées en 60 min. De même, l'activité enzymatique des virus aviaires n'est pas totalement abolie après 30 min à pH 3,0 alors que 15 min à pH 5,0 suffisent à inactiver toutes les souches de mammifères.</ce:simple-para><ce:simple-para>Ces différences de comportement peuvent être mises en rapport avec l'adaptation nécessaire de ces virus à leurs hôtes. La température interne des oiseaux est supérieure à 40°C et, chez eux, le virus grippal a un tropisme entérique, ce qui implique un passage par de segments du tube digestif où le pH est très acide.</ce:simple-para><ce:simple-para>Ces résultats ne contredisent cependant pas les hypothèses de filiation entre les orthomyxovirus d'oiseaux et ceux de mammifères.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword" xml:lang="en"><ce:section-title>Key-words</ce:section-title><ce:keyword><ce:text><ce:italic>Orthomyxovirus</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Influenza virus</ce:text></ce:keyword><ce:keyword><ce:text>A(H1N1) subtype</ce:text></ce:keyword><ce:keyword><ce:text>Physicochemical criteria</ce:text></ce:keyword><ce:keyword><ce:text>Birds</ce:text></ce:keyword><ce:keyword><ce:text>Mammals</ce:text></ce:keyword></ce:keywords><ce:keywords class="keyword" xml:lang="fr"><ce:section-title>Mots-clés</ce:section-title><ce:keyword><ce:text><ce:italic>Orthomyxovirus</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Virus grippal</ce:text></ce:keyword><ce:keyword><ce:text>Critères physico-chimiques</ce:text></ce:keyword><ce:keyword><ce:text>Oiseaux</ce:text></ce:keyword><ce:keyword><ce:text>Mammifères</ce:text></ce:keyword><ce:keyword><ce:text>Sous-type A(H1N1)</ce:text></ce:keyword></ce:keywords></head><tail><ce:bibliography><ce:section-title>References</ce:section-title><ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>Breuning and Scholtissek, 1974</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Breuning</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Scholtissek</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>A reassortant between influenza A viruses (H7N2) synthesizing an enzymatically inactive neuraminidase at 40°C which is not incorporated into infectious particles</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Virology</sb:maintitle></sb:title><sb:volume-nr>150</sb:volume-nr></sb:series><sb:date>1974</sb:date></sb:issue><sb:pages><sb:first-page>65</sb:first-page><sb:last-page>74</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib2"><ce:label>Cabezas, et al, 1983</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Cabezas</ce:surname><ce:given-name>J.A.</ce:given-name></sb:author><sb:author><ce:surname>Reglero</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Hannoun</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>A fluorimetric procedure for measuring the neuraminidase activity: its application to the determination of this activity in influenza and parainfluenza viruses</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Analyt. 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Merced 1, 37008 Salamanca (Spain)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Villar</namePart><affiliation>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.A.</namePart><namePart type="family">Cabezas</namePart><affiliation>Department of Biochemistry and Molecular Biology, University of Salamanca, Pl. Merced 1, 37008 Salamanca (Spain)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Summary: The study of biological properties of influenza virus strains belonging to the same subtype A(H1N1) and closely antigenically related, but isolated from different animal species (man, pig and duck), demonstrated that avian strains were more resistant than those isolated from mammals to high temperature and low pH, as shown by titration of residual infectivity in cell cultures (MDCK) and by sialidase assay.The difference in behaviour could be correlated to biological adaptation of the virus to its host. Avian body temperature is 40°C and influenza virus, in ducks, is enterotropic and therefore capable of passing through the low pH values in the upper digestive tract of the animal.These results do not contradict the hypothesis of a possible filiation between avian and mammalian orthomyxoviruses.</abstract><abstract lang="fr">Résumé: L'étude des propriétés biologiques et physiques de souches de virus grippaux appartenant au sérotype A(H1N1) isolées d'espèces différentes (homme, porc ou oiseaux) a permis la mise en évidence d'importantes différences. Les souches d'origine aviaire se révèlent plus résistantes à l'action inactivante de la chaleur et des pH acides ainsi que le démontrent les résultats des titrages de pouvoir infectant et les épreuves d'activité de la sialidase. A 40°C, l'activité de la sialidase des souches aviaires n'est pas altérée, même après 90 min d'exposition, alors que la plupart de celles de l'homme ou du porc sont totalement inactivées en 60 min. De même, l'activité enzymatique des virus aviaires n'est pas totalement abolie après 30 min à pH 3,0 alors que 15 min à pH 5,0 suffisent à inactiver toutes les souches de mammifères.Ces différences de comportement peuvent être mises en rapport avec l'adaptation nécessaire de ces virus à leurs hôtes. La température interne des oiseaux est supérieure à 40°C et, chez eux, le virus grippal a un tropisme entérique, ce qui implique un passage par de segments du tube digestif où le pH est très acide.Ces résultats ne contredisent cependant pas les hypothèses de filiation entre les orthomyxovirus d'oiseaux et ceux de mammifères.</abstract><subject lang="en"><genre>Key-words</genre><topic>Orthomyxovirus</topic><topic>Influenza virus</topic><topic>A(H1N1) subtype</topic><topic>Physicochemical criteria</topic><topic>Birds</topic><topic>Mammals</topic></subject><subject lang="fr"><genre>Mots-clés</genre><topic>Orthomyxovirus</topic><topic>Virus grippal</topic><topic>Critères physico-chimiques</topic><topic>Oiseaux</topic><topic>Mammifères</topic><topic>Sous-type A(H1N1)</topic></subject><relatedItem type="host"><titleInfo><title>Research in Virology</title></titleInfo><titleInfo type="abbreviated"><title>X135</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued></originInfo><identifier type="ISSN">0923-2516</identifier><identifier type="PII">S0923-2516(89)X8001-3</identifier><part><date>1989</date><detail type="volume"><number>140</number><caption>vol.</caption></detail><detail type="supplement"><number>C</number><caption>Suppl.</caption></detail><extent unit="issue-pages"><start>5</start><end>623</end></extent><extent unit="pages"><start>395</start><end>404</end></extent></part></relatedItem><identifier type="istex">F17340AB9BF47A5FB3079275005B31037ADC71F9</identifier><identifier type="ark">ark:/67375/6H6-5D4RSTW8-1</identifier><identifier type="DOI">10.1016/S0923-2516(89)80118-9</identifier><identifier type="PII">S0923-2516(89)80118-9</identifier><identifier type="ArticleID">80118</identifier><accessCondition type="use and reproduction" contentType="copyright">©1989 Elsevier</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Elsevier, ©1989</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5D4RSTW8-1/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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