Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2
Identifieur interne : 000143 ( Istex/Corpus ); précédent : 000142; suivant : 000144Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2
Auteurs : Seyyed Mahmoud Ebrahimi ; Majid Tebianian ; Khosrow Aghaiypour ; Hassan Nili ; Ali MirjaliliSource :
- Molecular Biology Reports [ 0301-4851 ] ; 2010-07-01.
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Abstract
Abstract: The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.
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DOI: 10.1007/s11033-009-9851-5
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ISTEX:3C7B3C5886657DD76BADC567CA649A180EE99947Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2</title><author><name sortKey="Ebrahimi, Seyyed Mahmoud" sort="Ebrahimi, Seyyed Mahmoud" uniqKey="Ebrahimi S" first="Seyyed Mahmoud" last="Ebrahimi">Seyyed Mahmoud Ebrahimi</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: smebrahimi@shirazu.ac.ir</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: s.ebrahimi@rvsri.ir</mods:affiliation></affiliation></author><author><name sortKey="Tebianian, Majid" sort="Tebianian, Majid" uniqKey="Tebianian M" first="Majid" last="Tebianian">Majid Tebianian</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation></author><author><name sortKey="Aghaiypour, Khosrow" sort="Aghaiypour, Khosrow" uniqKey="Aghaiypour K" first="Khosrow" last="Aghaiypour">Khosrow Aghaiypour</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation></author><author><name sortKey="Nili, Hassan" sort="Nili, Hassan" uniqKey="Nili H" first="Hassan" last="Nili">Hassan Nili</name><affiliation><mods:affiliation>Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz, Iran</mods:affiliation></affiliation></author><author><name sortKey="Mirjalili, Ali" sort="Mirjalili, Ali" uniqKey="Mirjalili A" first="Ali" last="Mirjalili">Ali Mirjalili</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3C7B3C5886657DD76BADC567CA649A180EE99947</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1007/s11033-009-9851-5</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-SJRQ299J-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000143</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000143</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2</title><author><name sortKey="Ebrahimi, Seyyed Mahmoud" sort="Ebrahimi, Seyyed Mahmoud" uniqKey="Ebrahimi S" first="Seyyed Mahmoud" last="Ebrahimi">Seyyed Mahmoud Ebrahimi</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: smebrahimi@shirazu.ac.ir</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: s.ebrahimi@rvsri.ir</mods:affiliation></affiliation></author><author><name sortKey="Tebianian, Majid" sort="Tebianian, Majid" uniqKey="Tebianian M" first="Majid" last="Tebianian">Majid Tebianian</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation></author><author><name sortKey="Aghaiypour, Khosrow" sort="Aghaiypour, Khosrow" uniqKey="Aghaiypour K" first="Khosrow" last="Aghaiypour">Khosrow Aghaiypour</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation></author><author><name sortKey="Nili, Hassan" sort="Nili, Hassan" uniqKey="Nili H" first="Hassan" last="Nili">Hassan Nili</name><affiliation><mods:affiliation>Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz, Iran</mods:affiliation></affiliation></author><author><name sortKey="Mirjalili, Ali" sort="Mirjalili, Ali" uniqKey="Mirjalili A" first="Ali" last="Mirjalili">Ali Mirjalili</name><affiliation><mods:affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Biology Reports</title><title level="j" type="sub">An International Journal on Molecular and Cellular Biology</title><title level="j" type="abbrev">Mol Biol Rep</title><idno type="ISSN">0301-4851</idno><idno type="eISSN">1573-4978</idno><imprint><publisher>Springer Netherlands</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2010-07-01">2010-07-01</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="2909">2909</biblScope><biblScope unit="page" to="2914">2914</biblScope></imprint><idno type="ISSN">0301-4851</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0301-4851</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Avian influenza</term><term>Expression</term><term>M2 protein</term><term>SDS-PAGE</term><term>pAED4</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Seyyed Mahmoud Ebrahimi</name><affiliations><json:string>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</json:string><json:string>E-mail: smebrahimi@shirazu.ac.ir</json:string><json:string>E-mail: s.ebrahimi@rvsri.ir</json:string></affiliations></json:item><json:item><name>Majid Tebianian</name><affiliations><json:string>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</json:string></affiliations></json:item><json:item><name>Khosrow Aghaiypour</name><affiliations><json:string>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</json:string></affiliations></json:item><json:item><name>Hassan Nili</name><affiliations><json:string>Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz, Iran</json:string></affiliations></json:item><json:item><name>Ali Mirjalili</name><affiliations><json:string>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>M2 protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pAED4</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SDS-PAGE</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Expression</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Avian influenza</value></json:item></subject><articleId><json:string>9851</json:string><json:string>s11033-009-9851-5</json:string></articleId><arkIstex>ark:/67375/VQC-SJRQ299J-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.</abstract><qualityIndicators><score>6.866</score><pdfWordCount>2910</pdfWordCount><pdfCharCount>18271</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>6</pdfPageCount><pdfPageSize>595.276 x 790.866 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>163</abstractWordCount><abstractCharCount>1157</abstractCharCount><keywordCount>5</keywordCount></qualityIndicators><title>Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2</title><genre><json:string>research-article</json:string></genre><host><title>Molecular Biology Reports</title><language><json:string>unknown</json:string></language><publicationDate>2010</publicationDate><copyrightDate>2010</copyrightDate><issn><json:string>0301-4851</json:string></issn><eissn><json:string>1573-4978</json:string></eissn><journalId><json:string>11033</json:string></journalId><volume>37</volume><issue>6</issue><pages><first>2909</first><last>2914</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Animal Biochemistry</value></json:item><json:item><value>Animal Anatomy / Morphology / Histology</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-SJRQ299J-V</json:string></ark><publicationDate>2010</publicationDate><copyrightDate>2009</copyrightDate><doi><json:string>10.1007/s11033-009-9851-5</json:string></doi><id>3C7B3C5886657DD76BADC567CA649A180EE99947</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-SJRQ299J-V/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-SJRQ299J-V/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/VQC-SJRQ299J-V/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; 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In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>M2 protein</term></item><item><term>pAED4</term></item><item><term>SDS-PAGE</term></item><item><term>Expression</term></item><item><term>Avian influenza</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Animal Biochemistry</term></item><item><term>Animal Anatomy / Morphology / Histology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2009-05-09">Created</change><change when="2010-07-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-SJRQ299J-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer Netherlands</PublisherName><PublisherLocation>Dordrecht</PublisherLocation></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11033</JournalID><JournalPrintISSN>0301-4851</JournalPrintISSN><JournalElectronicISSN>1573-4978</JournalElectronicISSN><JournalTitle>Molecular Biology Reports</JournalTitle><JournalSubTitle>An International Journal on Molecular and Cellular Biology</JournalSubTitle><JournalAbbreviatedTitle>Mol Biol Rep</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Animal Biochemistry</JournalSubject><JournalSubject Type="Secondary">Animal Anatomy / Morphology / Histology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>37</VolumeIDStart><VolumeIDEnd>37</VolumeIDEnd><VolumeIssueCount>8</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>6</IssueIDStart><IssueIDEnd>6</IssueIDEnd><IssueArticleCount>60</IssueArticleCount><IssueHistory><OnlineDate><Year>2010</Year><Month>7</Month><Day>8</Day></OnlineDate><PrintDate><Year>2010</Year><Month>7</Month><Day>7</Day></PrintDate><CoverDate><Year>2010</Year><Month>7</Month></CoverDate><PricelistYear>2010</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2010</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s11033-009-9851-5" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>9851</ArticleID><ArticleDOI>10.1007/s11033-009-9851-5</ArticleDOI><ArticleSequenceNumber>43</ArticleSequenceNumber><ArticleTitle Language="En" OutputMedium="All">Prokaryotic expression and characterization of avian influenza A virus <Emphasis Type="Italic">M2</Emphasis> gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2</ArticleTitle><ArticleFirstPage>2909</ArticleFirstPage><ArticleLastPage>2914</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2009</Year><Month>9</Month><Day>28</Day></RegistrationDate><Received><Year>2009</Year><Month>5</Month><Day>9</Day></Received><Accepted><Year>2009</Year><Month>9</Month><Day>28</Day></Accepted><OnlineDate><Year>2009</Year><Month>10</Month><Day>7</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media B.V.</CopyrightHolderName><CopyrightYear>2009</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Seyyed</GivenName><GivenName>Mahmoud</GivenName><FamilyName>Ebrahimi</FamilyName></AuthorName><Contact><Phone>+98-261-4570038</Phone><Fax>+98-261-4552194</Fax><Email>smebrahimi@shirazu.ac.ir</Email><Email>s.ebrahimi@rvsri.ir</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Majid</GivenName><FamilyName>Tebianian</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Khosrow</GivenName><FamilyName>Aghaiypour</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Hassan</GivenName><FamilyName>Nili</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Ali</GivenName><FamilyName>Mirjalili</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Department of Biotechnology</OrgDivision><OrgName>Razi Vaccine and Serum Research Institute</OrgName><OrgAddress><City>Karaj</City><State>Tehran</State><Country Code="IR">Iran</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Avian Medicine, School of Veterinary Medicine</OrgDivision><OrgName>Shiraz University</OrgName><OrgAddress><City>Shiraz</City><Country Code="IR">Iran</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) <Emphasis Type="Italic">M2</Emphasis> gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in <Emphasis Type="Italic">E.</Emphasis> <Emphasis Type="Italic">coli</Emphasis> BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.
</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>M2 protein</Keyword><Keyword>pAED4</Keyword><Keyword>SDS-PAGE</Keyword><Keyword>Expression</Keyword><Keyword>Avian influenza</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Seyyed</namePart><namePart type="given">Mahmoud</namePart><namePart type="family">Ebrahimi</namePart><affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</affiliation><affiliation>E-mail: smebrahimi@shirazu.ac.ir</affiliation><affiliation>E-mail: s.ebrahimi@rvsri.ir</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Majid</namePart><namePart type="family">Tebianian</namePart><affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Khosrow</namePart><namePart type="family">Aghaiypour</namePart><affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hassan</namePart><namePart type="family">Nili</namePart><affiliation>Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ali</namePart><namePart type="family">Mirjalili</namePart><affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Netherlands</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateCreated encoding="w3cdtf">2009-05-09</dateCreated><dateIssued encoding="w3cdtf">2010-07-01</dateIssued><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.</abstract><subject lang="en"><genre>Keywords</genre><topic>M2 protein</topic><topic>pAED4</topic><topic>SDS-PAGE</topic><topic>Expression</topic><topic>Avian influenza</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Biology Reports</title><subTitle>An International Journal on Molecular and Cellular Biology</subTitle></titleInfo><titleInfo type="abbreviated"><title>Mol Biol Rep</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2010-07-08</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Animal Biochemistry</topic><topic>Animal Anatomy / Morphology / Histology</topic></subject><identifier type="ISSN">0301-4851</identifier><identifier type="eISSN">1573-4978</identifier><identifier type="JournalID">11033</identifier><identifier type="IssueArticleCount">60</identifier><identifier type="VolumeIssueCount">8</identifier><part><date>2010</date><detail type="volume"><number>37</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>2909</start><end>2914</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media B.V., 2010</recordOrigin></recordInfo></relatedItem><identifier type="istex">3C7B3C5886657DD76BADC567CA649A180EE99947</identifier><identifier type="ark">ark:/67375/VQC-SJRQ299J-V</identifier><identifier type="DOI">10.1007/s11033-009-9851-5</identifier><identifier type="ArticleID">9851</identifier><identifier type="ArticleID">s11033-009-9851-5</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media B.V., 2009</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media B.V., 2009</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-SJRQ299J-V/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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