Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2
Identifieur interne : 000143 ( Istex/Corpus ); précédent : 000142; suivant : 000144Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2
Auteurs : Seyyed Mahmoud Ebrahimi ; Majid Tebianian ; Khosrow Aghaiypour ; Hassan Nili ; Ali MirjaliliSource :
- Molecular Biology Reports [ 0301-4851 ] ; 2010-07-01.
English descriptors
- KwdEn :
Abstract
Abstract: The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.
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DOI: 10.1007/s11033-009-9851-5
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<Affiliation ID="Aff1"><OrgDivision>Department of Biotechnology</OrgDivision>
<OrgName>Razi Vaccine and Serum Research Institute</OrgName>
<OrgAddress><City>Karaj</City>
<State>Tehran</State>
<Country Code="IR">Iran</Country>
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</Affiliation>
<Affiliation ID="Aff2"><OrgDivision>Department of Avian Medicine, School of Veterinary Medicine</OrgDivision>
<OrgName>Shiraz University</OrgName>
<OrgAddress><City>Shiraz</City>
<Country Code="IR">Iran</Country>
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<Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading>
<Para>The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) <Emphasis Type="Italic">M2</Emphasis>
gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in <Emphasis Type="Italic">E.</Emphasis>
<Emphasis Type="Italic">coli</Emphasis>
BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.
</Para>
</Abstract>
<KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading>
<Keyword>M2 protein</Keyword>
<Keyword>pAED4</Keyword>
<Keyword>SDS-PAGE</Keyword>
<Keyword>Expression</Keyword>
<Keyword>Avian influenza</Keyword>
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<titleInfo type="alternative" contentType="CDATA"><title>Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2</title>
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<name type="personal" displayLabel="corresp"><namePart type="given">Seyyed</namePart>
<namePart type="given">Mahmoud</namePart>
<namePart type="family">Ebrahimi</namePart>
<affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</affiliation>
<affiliation>E-mail: smebrahimi@shirazu.ac.ir</affiliation>
<affiliation>E-mail: s.ebrahimi@rvsri.ir</affiliation>
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<name type="personal"><namePart type="given">Majid</namePart>
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<affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</affiliation>
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<name type="personal"><namePart type="given">Khosrow</namePart>
<namePart type="family">Aghaiypour</namePart>
<affiliation>Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Tehran, Iran</affiliation>
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<name type="personal"><namePart type="given">Hassan</namePart>
<namePart type="family">Nili</namePart>
<affiliation>Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, Shiraz, Iran</affiliation>
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<name type="personal"><namePart type="given">Ali</namePart>
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<abstract lang="en">Abstract: The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.</abstract>
<subject lang="en"><genre>Keywords</genre>
<topic>M2 protein</topic>
<topic>pAED4</topic>
<topic>SDS-PAGE</topic>
<topic>Expression</topic>
<topic>Avian influenza</topic>
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<dateIssued encoding="w3cdtf">2010-07-08</dateIssued>
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<subject><genre>Life Sciences</genre>
<topic>Animal Biochemistry</topic>
<topic>Animal Anatomy / Morphology / Histology</topic>
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<identifier type="ISSN">0301-4851</identifier>
<identifier type="eISSN">1573-4978</identifier>
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