The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses
Identifieur interne : 000114 ( Istex/Corpus ); précédent : 000113; suivant : 000115The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses
Auteurs : Kathryn L. Deshpande ; Clayton W. Naeve ; Robert G. WebsterSource :
- Virology [ 0042-6822 ] ; 1985.
English descriptors
- Teeft :
- Acid deletion, Amino, Amino acid, Amino acid change, Amino acid changes, Amino acid sequence, Amino acids, Antigenic, Antigenic analysis, Antigenic sites, Antigenically, Avian, Avian influenza viruses, Avian source, Avian viruses, Avirulent, Blok, Deletion, Deshpande, Gene constellation, Hemagglutinin, Homology, Influenza, Influenza virus, Influenza virus neuraminidase, Influenza viruses, Laver, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Naeve, Natl acad, Neuraminidase, Neuraminidase gene, Nucleotide, Nucleotide sequence, Other viruses, Rott, Scholtissek, Viral, Viral membrane, Virology, Virulent, Virulent chick, Virulent virus, Virulent viruses, Virus, Webster.
Abstract
Abstract: To define the sequence changes that occurred in an avian influenza virus neuraminidase (NA) during the evolution of virulence, we have studied the NA of the virulent and avirulent A/Chick/Penn/83 (H5N2) influenza viruses. A comparison of the deduced amino acid sequence from these viruses shows that the virulent strain, which evolved from the avirulent by the accumulation of point mutations (Bean et al., 1985), acquired four amino acid changes in the NA: one in the transmembrane segment, one in the stalk, and two in the head. A comparison of the deduced amino acid sequences with those of the human N2 NAs indicates a 20-amino acid deletion in the stalk of the Chick/Penn/83 NA. Antigenic analysis of the NAs from the avirulent and virulent Chick/Penn/83 virus shows they are antigenically very closely related, but can be distinguished with two monoclonal antibodies at a site which probably involves at least one of the amino acid changes in the NA head. Antigenic analysis also shows the Chick/Penn/83 NAs are closely related to the NAs of other N2 avian influenza viruses isolated between 1965 and 1984, supporting previous studies which indicate a relative antigenic stability of the NA among avian N2 influenza viruses. The Chick/Penn/83 NAs are the first N2 NA genes of an avian virus to be sequenced. These NAs are antigenically closely related to the 1957 human N2 NAs, and show a high degree of amino acid sequence homology with the prototype 1957 human N2 NA. These data give further support to the view that the 1957 human H2N2 viruses were at least partially derived from an avian source.
Url:
DOI: 10.1016/0042-6822(85)90226-0
Links to Exploration step
ISTEX:98890D352E6201C3F1A934CF5C78E3B7B3C36F27Le document en format XML
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Box 318, Memphis, Tennessee 38101, USA</mods:affiliation></affiliation></author><author><name sortKey="Webster, Robert G" sort="Webster, Robert G" uniqKey="Webster R" first="Robert G." last="Webster">Robert G. Webster</name><affiliation><mods:affiliation>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. 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A comparison of the deduced amino acid sequence from these viruses shows that the virulent strain, which evolved from the avirulent by the accumulation of point mutations (Bean et al., 1985), acquired four amino acid changes in the NA: one in the transmembrane segment, one in the stalk, and two in the head. A comparison of the deduced amino acid sequences with those of the human N2 NAs indicates a 20-amino acid deletion in the stalk of the Chick/Penn/83 NA. Antigenic analysis of the NAs from the avirulent and virulent Chick/Penn/83 virus shows they are antigenically very closely related, but can be distinguished with two monoclonal antibodies at a site which probably involves at least one of the amino acid changes in the NA head. Antigenic analysis also shows the Chick/Penn/83 NAs are closely related to the NAs of other N2 avian influenza viruses isolated between 1965 and 1984, supporting previous studies which indicate a relative antigenic stability of the NA among avian N2 influenza viruses. The Chick/Penn/83 NAs are the first N2 NA genes of an avian virus to be sequenced. These NAs are antigenically closely related to the 1957 human N2 NAs, and show a high degree of amino acid sequence homology with the prototype 1957 human N2 NA. These data give further support to the view that the 1957 human H2N2 viruses were at least partially derived from an avian source.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>neuraminidase</json:string><json:string>avian</json:string><json:string>antigenic</json:string><json:string>avirulent</json:string><json:string>monoclonal</json:string><json:string>virology</json:string><json:string>monoclonal antibodies</json:string><json:string>deletion</json:string><json:string>amino</json:string><json:string>virulent</json:string><json:string>influenza</json:string><json:string>laver</json:string><json:string>influenza viruses</json:string><json:string>homology</json:string><json:string>influenza virus</json:string><json:string>antigenically</json:string><json:string>naeve</json:string><json:string>blok</json:string><json:string>rott</json:string><json:string>antigenic analysis</json:string><json:string>amino acids</json:string><json:string>viral</json:string><json:string>amino acid sequence</json:string><json:string>hemagglutinin</json:string><json:string>scholtissek</json:string><json:string>deshpande</json:string><json:string>virulent virus</json:string><json:string>amino acid changes</json:string><json:string>virus</json:string><json:string>other viruses</json:string><json:string>acid deletion</json:string><json:string>virulent viruses</json:string><json:string>neuraminidase gene</json:string><json:string>avian influenza viruses</json:string><json:string>influenza virus neuraminidase</json:string><json:string>nucleotide sequence</json:string><json:string>nucleotide</json:string><json:string>webster</json:string><json:string>virulent chick</json:string><json:string>monoclonal antibody</json:string><json:string>avian viruses</json:string><json:string>amino acid change</json:string><json:string>viral membrane</json:string><json:string>gene constellation</json:string><json:string>antigenic sites</json:string><json:string>natl acad</json:string><json:string>amino acid</json:string><json:string>avian source</json:string></teeft></keywords><author><json:item><name>Kathryn L. 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Antigenic analysis of the NAs from the avirulent and virulent Chick/Penn/83 virus shows they are antigenically very closely related, but can be distinguished with two monoclonal antibodies at a site which probably involves at least one of the amino acid changes in the NA head. Antigenic analysis also shows the Chick/Penn/83 NAs are closely related to the NAs of other N2 avian influenza viruses isolated between 1965 and 1984, supporting previous studies which indicate a relative antigenic stability of the NA among avian N2 influenza viruses. The Chick/Penn/83 NAs are the first N2 NA genes of an avian virus to be sequenced. These NAs are antigenically closely related to the 1957 human N2 NAs, and show a high degree of amino acid sequence homology with the prototype 1957 human N2 NA. These data give further support to the view that the 1957 human H2N2 viruses were at least partially derived from an avian source.</abstract><qualityIndicators><score>10</score><pdfWordCount>5150</pdfWordCount><pdfCharCount>36521</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>504 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>268</abstractWordCount><abstractCharCount>1616</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses</title><pmid><json:string>2414922</json:string></pmid><pii><json:string>0042-6822(85)90226-0</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. 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(1979)</json:string><json:string>Bosch et al., 1979</json:string><json:string>Webster et al., 1982</json:string><json:string>Rott et ah, 1976, 1979</json:string><json:string>Elleman et al., 1982</json:string><json:string>Blok and Air, 1982b</json:string><json:string>Webster et al. (1982)</json:string><json:string>Kilbourne et al., 1968</json:string><json:string>Aymard-Henry et al. (1973)</json:string><json:string>Steuler et al. (1984)</json:string><json:string>Amit et al., 1985</json:string><json:string>Maxam and Gilbert, 1977</json:string><json:string>Rott et ab, 1976</json:string><json:string>Webster, 1972</json:string><json:string>October 1983</json:string><json:string>Blok and Air, 1982a</json:string><json:string>Jackson and Webster (1982)</json:string><json:string>Bean et oL, 1935</json:string><json:string>Ward et al. (1982)</json:string><json:string>Steuler et aL, 1984</json:string><json:string>Rott et ah, 1979</json:string><json:string>Bean et ab, 1980</json:string><json:string>Elleman et aL, 1982</json:string><json:string>Kawaoka et al. (1984)</json:string><json:string>van Rompuy et al., 1982</json:string><json:string>Bean and Webster, 1978</json:string><json:string>Air et al., 1985</json:string><json:string>Kohler and Milstein (1976)</json:string><json:string>Scholtissek et al., 1978</json:string><json:string>Bean et al., 1980</json:string><json:string>Martinez et aL, 1983</json:string><json:string>April and October 1983</json:string><json:string>Varghese et al, 1983</json:string><json:string>Bean et al., 1985</json:string><json:string>April 1983</json:string><json:string>Bentley and Brownlee, 1982</json:string><json:string>Seto and Rott, 1966</json:string><json:string>Bean et al, 1985</json:string><json:string>Sugiura and Ueda, 1980</json:string><json:string>Laver and Webster, 1972</json:string><json:string>Scholtissek et al., 1977</json:string><json:string>Steuler et al., 1984</json:string><json:string>Staden, 1982</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-1WF8SBVZ-T</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1985</publicationDate><copyrightDate>1985</copyrightDate><doi><json:string>10.1016/0042-6822(85)90226-0</json:string></doi><id>98890D352E6201C3F1A934CF5C78E3B7B3C36F27</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-1WF8SBVZ-T/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-1WF8SBVZ-T/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-1WF8SBVZ-T/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1985</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses</title><author xml:id="author-0000"><persName><forename type="first">Kathryn L.</forename><surname>Deshpande</surname></persName><affiliation>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. Box 318, Memphis, Tennessee 38101, USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Clayton W.</forename><surname>Naeve</surname></persName><affiliation>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. Box 318, Memphis, Tennessee 38101, USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Robert G.</forename><surname>Webster</surname></persName><note type="biography">Author to whom requests for reprints should be</note><affiliation>Author to whom requests for reprints should be</affiliation><affiliation>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. Box 318, Memphis, Tennessee 38101, USA</affiliation></author><idno type="istex">98890D352E6201C3F1A934CF5C78E3B7B3C36F27</idno><idno type="ark">ark:/67375/6H6-1WF8SBVZ-T</idno><idno type="DOI">10.1016/0042-6822(85)90226-0</idno><idno type="PII">0042-6822(85)90226-0</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0659-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985"></date><biblScope unit="volume">147</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="49">49</biblScope><biblScope unit="page" to="60">60</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1985</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: To define the sequence changes that occurred in an avian influenza virus neuraminidase (NA) during the evolution of virulence, we have studied the NA of the virulent and avirulent A/Chick/Penn/83 (H5N2) influenza viruses. A comparison of the deduced amino acid sequence from these viruses shows that the virulent strain, which evolved from the avirulent by the accumulation of point mutations (Bean et al., 1985), acquired four amino acid changes in the NA: one in the transmembrane segment, one in the stalk, and two in the head. A comparison of the deduced amino acid sequences with those of the human N2 NAs indicates a 20-amino acid deletion in the stalk of the Chick/Penn/83 NA. Antigenic analysis of the NAs from the avirulent and virulent Chick/Penn/83 virus shows they are antigenically very closely related, but can be distinguished with two monoclonal antibodies at a site which probably involves at least one of the amino acid changes in the NA head. Antigenic analysis also shows the Chick/Penn/83 NAs are closely related to the NAs of other N2 avian influenza viruses isolated between 1965 and 1984, supporting previous studies which indicate a relative antigenic stability of the NA among avian N2 influenza viruses. The Chick/Penn/83 NAs are the first N2 NA genes of an avian virus to be sequenced. These NAs are antigenically closely related to the 1957 human N2 NAs, and show a high degree of amino acid sequence homology with the prototype 1957 human N2 NA. These data give further support to the view that the 1957 human H2N2 viruses were at least partially derived from an avian source.</p></abstract></profileDesc><revisionDesc><change when="1985">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-1WF8SBVZ-T/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>85902260</aid><ce:pii>0042-6822(85)90226-0</ce:pii><ce:doi>10.1016/0042-6822(85)90226-0</ce:doi><ce:copyright type="unknown" year="1985"></ce:copyright></item-info><head><ce:title>The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses</ce:title><ce:author-group><ce:author><ce:given-name>Kathryn L.</ce:given-name><ce:surname>Deshpande</ce:surname></ce:author><ce:author><ce:given-name>Clayton W.</ce:given-name><ce:surname>Naeve</ce:surname></ce:author><ce:author><ce:given-name>Robert G.</ce:given-name><ce:surname>Webster</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. Box 318, Memphis, Tennessee 38101, USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>Author to whom requests for reprints should be</ce:text></ce:correspondence></ce:author-group><ce:date-received day="28" month="5" year="1985"></ce:date-received><ce:date-accepted day="8" month="7" year="1985"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>To define the sequence changes that occurred in an avian influenza virus neuraminidase (NA) during the evolution of virulence, we have studied the NA of the virulent and avirulent A/Chick/Penn/83 (H5N2) influenza viruses. A comparison of the deduced amino acid sequence from these viruses shows that the virulent strain, which evolved from the avirulent by the accumulation of point mutations (Bean <ce:italic>et al.</ce:italic>, 1985), acquired four amino acid changes in the NA: one in the transmembrane segment, one in the stalk, and two in the head. A comparison of the deduced amino acid sequences with those of the human N2 NAs indicates a 20-amino acid deletion in the stalk of the Chick/Penn/83 NA. Antigenic analysis of the NAs from the avirulent and virulent Chick/Penn/83 virus shows they are antigenically very closely related, but can be distinguished with two monoclonal antibodies at a site which probably involves at least one of the amino acid changes in the NA head. Antigenic analysis also shows the Chick/Penn/83 NAs are closely related to the NAs of other N2 avian influenza viruses isolated between 1965 and 1984, supporting previous studies which indicate a relative antigenic stability of the NA among avian N2 influenza viruses. The Chick/Penn/83 NAs are the first N2 NA genes of an avian virus to be sequenced. These NAs are antigenically closely related to the 1957 human N2 NAs, and show a high degree of amino acid sequence homology with the prototype 1957 human N2 NA. These data give further support to the view that the 1957 human H2N2 viruses were at least partially derived from an avian source.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The neuraminidases of the virulent and avirulent A/Chicken/Pennsylvania/ 83 (H5N2) influenza a viruses: Sequence and antigenic analyses</title></titleInfo><name type="personal"><namePart type="given">Kathryn L.</namePart><namePart type="family">Deshpande</namePart><affiliation>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. Box 318, Memphis, Tennessee 38101, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Clayton W.</namePart><namePart type="family">Naeve</namePart><affiliation>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. Box 318, Memphis, Tennessee 38101, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Robert G.</namePart><namePart type="family">Webster</namePart><affiliation>St. Jude Children's Research Hospital, Department of Virology and Molecular Biology, 332 N. Lauderdale, P.O. Box 318, Memphis, Tennessee 38101, USA</affiliation><description>Author to whom requests for reprints should be</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued><copyrightDate encoding="w3cdtf">1985</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: To define the sequence changes that occurred in an avian influenza virus neuraminidase (NA) during the evolution of virulence, we have studied the NA of the virulent and avirulent A/Chick/Penn/83 (H5N2) influenza viruses. A comparison of the deduced amino acid sequence from these viruses shows that the virulent strain, which evolved from the avirulent by the accumulation of point mutations (Bean et al., 1985), acquired four amino acid changes in the NA: one in the transmembrane segment, one in the stalk, and two in the head. A comparison of the deduced amino acid sequences with those of the human N2 NAs indicates a 20-amino acid deletion in the stalk of the Chick/Penn/83 NA. Antigenic analysis of the NAs from the avirulent and virulent Chick/Penn/83 virus shows they are antigenically very closely related, but can be distinguished with two monoclonal antibodies at a site which probably involves at least one of the amino acid changes in the NA head. Antigenic analysis also shows the Chick/Penn/83 NAs are closely related to the NAs of other N2 avian influenza viruses isolated between 1965 and 1984, supporting previous studies which indicate a relative antigenic stability of the NA among avian N2 influenza viruses. The Chick/Penn/83 NAs are the first N2 NA genes of an avian virus to be sequenced. These NAs are antigenically closely related to the 1957 human N2 NAs, and show a high degree of amino acid sequence homology with the prototype 1957 human N2 NA. These data give further support to the view that the 1957 human H2N2 viruses were at least partially derived from an avian source.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0659-9</identifier><part><date>1985</date><detail type="volume"><number>147</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>242</end></extent><extent unit="pages"><start>49</start><end>60</end></extent></part></relatedItem><identifier type="istex">98890D352E6201C3F1A934CF5C78E3B7B3C36F27</identifier><identifier type="ark">ark:/67375/6H6-1WF8SBVZ-T</identifier><identifier type="DOI">10.1016/0042-6822(85)90226-0</identifier><identifier type="PII">0042-6822(85)90226-0</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-1WF8SBVZ-T/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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