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Comparison of neuraminidases of the same subtype but from different species using a new method of titration

Identifieur interne : 000044 ( Istex/Corpus ); précédent : 000043; suivant : 000045

Comparison of neuraminidases of the same subtype but from different species using a new method of titration

Auteurs : B. Fiszon ; C. Hannoun

Source :

RBID : ISTEX:FB607767D382D4F3B0C37AA73D90A67B95B4A589

English descriptors

Abstract

Abstract: Neuraminidase is one of the two surface glycoproteins of influenza virions. In order to compare neuraminidases of the same subtype but isolated from different species (man, birds, pig), a new and simple method was adapted and optimized using peanut hemagglutinin. Results were very similar to those obtained with the classical method recommended by the WHO, using fetuin as a substrate. The technique was used to examine the relationship between animal and human neuraminidases belonging to serotypes N1 and N2. The resulte confirm the possible role of ducks as a reservoir for influenza viruses and the eventuality of interspecific exchanges.

Url:
DOI: 10.1016/0166-0934(90)90148-9

Links to Exploration step

ISTEX:FB607767D382D4F3B0C37AA73D90A67B95B4A589

Le document en format XML

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<abstract lang="en">Abstract: Neuraminidase is one of the two surface glycoproteins of influenza virions. In order to compare neuraminidases of the same subtype but isolated from different species (man, birds, pig), a new and simple method was adapted and optimized using peanut hemagglutinin. Results were very similar to those obtained with the classical method recommended by the WHO, using fetuin as a substrate. The technique was used to examine the relationship between animal and human neuraminidases belonging to serotypes N1 and N2. The resulte confirm the possible role of ducks as a reservoir for influenza viruses and the eventuality of interspecific exchanges.</abstract>
<subject>
<genre>Keywords</genre>
<topic>Influenza virus</topic>
<topic>Neuraminidase</topic>
<topic>Neuraminidase assay</topic>
<topic>Peanut hemagglutinin</topic>
<topic>Wild duck</topic>
</subject>
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<title>Journal of Virological Methods</title>
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<title>VIRMET</title>
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<originInfo>
<publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1990</dateIssued>
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<identifier type="ISSN">0166-0934</identifier>
<identifier type="PII">S0166-0934(00)X0154-1</identifier>
<part>
<date>1990</date>
<detail type="volume">
<number>27</number>
<caption>vol.</caption>
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<detail type="issue">
<number>1</number>
<caption>no.</caption>
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<extent unit="issue-pages">
<start>1</start>
<end>124</end>
</extent>
<extent unit="pages">
<start>79</start>
<end>90</end>
</extent>
</part>
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<identifier type="ark">ark:/67375/6H6-1FK54WQZ-P</identifier>
<identifier type="DOI">10.1016/0166-0934(90)90148-9</identifier>
<identifier type="PII">0166-0934(90)90148-9</identifier>
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