Natural and ‘ In vitro ’ selected antigenic variants of influenza A virus (H2N2)
Identifieur interne : 000014 ( Istex/Corpus ); précédent : 000013; suivant : 000015Natural and ‘ In vitro ’ selected antigenic variants of influenza A virus (H2N2)
Auteurs : A. Natali ; E. Pilotti ; P. O. Valcavi ; C. Chezzi ; J. S. OxfordSource :
- Journal of Infection [ 0163-4453 ] ; 1998.
English descriptors
- Teeft :
- Antibody, Antigenic, Antigenic site, Antigenic sites, Antigenic variant, Antigenic variants, Antigenic variation, Epitope, Haemolysis, Haemolysis area, Influenza, Influenza infection, Influenza virus, Influenza virus hemagglutinin, Influenza viruses, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoclonal antibody pressure, Natural variant, Natural variants, Original antigenic, Pandemic influenza, Parental, Parental virus, Positive sera, Previous studies, Serological, Serological analysis, Serological reactivity, Single epitope, Unilateral test, Variant, Virus, World health organization.
Abstract
We provide data on the prevalence of SRH antibody to influenza A/Singapore/1/57 (H2N2). Approximately 10.3% of sera had antibody to the influenza A (H2N2) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H3N2) and 31.5% to influenza A (H2N1) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H2N2) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H2N2) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in vitro variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.
Url:
DOI: 10.1016/S0163-4453(98)90260-2
Links to Exploration step
ISTEX:CAB58BDF7560E54BB40F3369FCD10F8C9667E297Le document en format XML
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Chezzi</name><affiliation><mods:affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</mods:affiliation></affiliation></author><author><name sortKey="Oxford, J S" sort="Oxford, J S" uniqKey="Oxford J" first="J. S." last="Oxford">J. S. Oxford</name><affiliation><mods:affiliation>Academic Virology, Department of Medical Microbilogy, St Bartholomew's and the Royal London School of Medicine, Turner Street, London E1 2AD, U.K.</mods:affiliation></affiliation><affiliation><mods:affiliation>Please address all correspondence to: J. S. 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Approximately 10.3% of sera had antibody to the influenza A (H2N2) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H3N2) and 31.5% to influenza A (H2N1) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H2N2) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H2N2) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in vitro variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>antigenic</json:string><json:string>monoclonal</json:string><json:string>antigenic variants</json:string><json:string>influenza</json:string><json:string>parental virus</json:string><json:string>haemolysis</json:string><json:string>serological reactivity</json:string><json:string>monoclonal antibodies</json:string><json:string>epitope</json:string><json:string>monoclonal antibody</json:string><json:string>natural variants</json:string><json:string>variant</json:string><json:string>haemolysis area</json:string><json:string>antigenic variant</json:string><json:string>positive sera</json:string><json:string>serological</json:string><json:string>virus</json:string><json:string>parental</json:string><json:string>world health organization</json:string><json:string>single epitope</json:string><json:string>influenza viruses</json:string><json:string>antibody</json:string><json:string>antigenic site</json:string><json:string>serological analysis</json:string><json:string>antigenic variation</json:string><json:string>antigenic sites</json:string><json:string>monoclonal antibody pressure</json:string><json:string>unilateral test</json:string><json:string>previous studies</json:string><json:string>original antigenic</json:string><json:string>pandemic influenza</json:string><json:string>influenza infection</json:string><json:string>influenza virus</json:string><json:string>influenza virus hemagglutinin</json:string><json:string>natural variant</json:string></teeft></keywords><author><json:item><name>A. Natali</name><affiliations><json:string>Institute of Microbiology, University of Parma, Parma 43100, Italy</json:string></affiliations></json:item><json:item><name>E. Pilotti</name><affiliations><json:string>Institute of Microbiology, University of Parma, Parma 43100, Italy</json:string></affiliations></json:item><json:item><name>P.O. Valcavi</name><affiliations><json:string>Institute of Microbiology, University of Parma, Parma 43100, Italy</json:string></affiliations></json:item><json:item><name>C. Chezzi</name><affiliations><json:string>Institute of Microbiology, University of Parma, Parma 43100, Italy</json:string></affiliations></json:item><json:item><name>J.S. Oxford</name><affiliations><json:string>Academic Virology, Department of Medical Microbilogy, St Bartholomew's and the Royal London School of Medicine, Turner Street, London E1 2AD, U.K.</json:string><json:string>Please address all correspondence to: J. S. 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An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in vitro variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.</abstract><qualityIndicators><score>7.963</score><pdfWordCount>3815</pdfWordCount><pdfCharCount>19571</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>5</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>179</abstractWordCount><abstractCharCount>1180</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Natural and ‘ In vitro ’ selected antigenic variants of influenza A virus (H2N2)</title><pmid><json:string>9733372</json:string></pmid><pii><json:string>S0163-4453(98)90260-2</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Journal of Infection</title><language><json:string>unknown</json:string></language><publicationDate>1998</publicationDate><issn><json:string>0163-4453</json:string></issn><pii><json:string>S0163-4453(00)X0120-X</json:string></pii><volume>37</volume><issue>1</issue><pages><first>19</first><last>23</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1981</json:string><json:string>1968</json:string><json:string>1993, 1695</json:string><json:string>1957</json:string><json:string>1883</json:string><json:string>1993</json:string><json:string>1969</json:string><json:string>1998</json:string><json:string>20th century</json:string><json:string>1900</json:string><json:string>1980</json:string><json:string>1967</json:string></date><geogName></geogName><orgName><json:string>National Institute for Medical Research, Mill Hill, London NW</json:string><json:string>Department of Medical</json:string><json:string>Institute of Microbiology of the University of Parma, Italy</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Hong Kong</json:string><json:string>R. 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Approximately 10.3% of sera had antibody to the influenza A (H2N2) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H3N2) and 31.5% to influenza A (H2N1) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H2N2) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H2N2) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in vitro variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.</p></abstract></profileDesc><revisionDesc><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-VM1V2P47-B/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="fla"><item-info><jid>YJINF</jid><aid>98902602</aid><ce:pii>S0163-4453(98)90260-2</ce:pii><ce:doi>10.1016/S0163-4453(98)90260-2</ce:doi><ce:copyright type="unknown" year="1998">The British Infection Society</ce:copyright></item-info><head><ce:dochead><ce:textfn>Original article</ce:textfn></ce:dochead><ce:title>Natural and ‘<ce:italic>In vitro</ce:italic>’ selected antigenic variants of influenza A virus (H<ce:inf loc="post">2</ce:inf>N<ce:inf loc="post">2</ce:inf>)</ce:title><ce:author-group><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Natali</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Pilotti</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>P.O.</ce:given-name><ce:surname>Valcavi</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C.</ce:given-name><ce:surname>Chezzi</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.S.</ce:given-name><ce:surname>Oxford</ce:surname><ce:cross-ref refid="aff2"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="cor1"><ce:sup>*</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:label>1</ce:label><ce:textfn>Institute of Microbiology, University of Parma, Parma 43100, Italy</ce:textfn></ce:affiliation><ce:affiliation id="aff2"><ce:label>2</ce:label><ce:textfn>Academic Virology, Department of Medical Microbilogy, St Bartholomew's and the Royal London School of Medicine, Turner Street, London E1 2AD, U.K.</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>*</ce:label><ce:text>Please address all correspondence to: J. S. Oxford.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="12" month="11" year="1997"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:abstract-sec><ce:simple-para>We provide data on the prevalence of SRH antibody to influenza A/Singapore/1/57 (H<ce:inf loc="post">2</ce:inf>N<ce:inf loc="post">2</ce:inf>). Approximately 10.3% of sera had antibody to the influenza A (H<ce:inf loc="post">2</ce:inf>N<ce:inf loc="post">2</ce:inf>) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H<ce:inf loc="post">3</ce:inf>N<ce:inf loc="post">2</ce:inf>) and 31.5% to influenza A (H<ce:inf loc="post">2</ce:inf>N<ce:inf loc="post">1</ce:inf>) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H<ce:inf loc="post">2</ce:inf>N<ce:inf loc="post">2</ce:inf>) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H<ce:inf loc="post">2</ce:inf>N<ce:inf loc="post">2</ce:inf>) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in <ce:italic>vitro</ce:italic> variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.</ce:simple-para></ce:abstract-sec></ce:abstract></head><tail><ce:bibliography><ce:section-title>References</ce:section-title><ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>1</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wiley</ce:surname><ce:given-name>DC</ce:given-name></sb:author><sb:author><ce:surname>Skehell</ce:surname><ce:given-name>JJ</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The structure and function of the hemagglutinin membrane glycoprotein in influenza virus</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Ann Rev Biochem</sb:maintitle></sb:title><sb:volume-nr>56</sb:volume-nr></sb:series><sb:date>1987</sb:date></sb:issue><sb:pages><sb:first-page>365</sb:first-page><sb:last-page>394</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib2"><ce:label>2</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Wilson</ce:surname><ce:given-name>IA</ce:given-name></sb:author><sb:author><ce:surname>Cox</ce:surname><ce:given-name>NJ</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Structural basis of immune recognition of influenza virus hemagglutinin</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Ann Rev Immunol</sb:maintitle></sb:title><sb:volume-nr>8</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>747</sb:first-page><sb:last-page>771</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib3"><ce:label>3</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yewdell</ce:surname><ce:given-name>JW</ce:given-name></sb:author><sb:author><ce:surname>Webster</ce:surname><ce:given-name>RG</ce:given-name></sb:author><sb:author><ce:surname>Gerhard</ce:surname><ce:given-name>WU</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Antigenic variation in three distinct determinants of an influenza type A haemogglutinin molecule</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle></sb:title><sb:volume-nr>279</sb:volume-nr></sb:series><sb:date>1979</sb:date></sb:issue><sb:pages><sb:first-page>246</sb:first-page><sb:last-page>248</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib4"><ce:label>4</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Pereira</ce:surname><ce:given-name>HG</ce:given-name></sb:author><sb:author><ce:surname>Pereira</ce:surname><ce:given-name>MS</ce:given-name></sb:author><sb:author><ce:surname>Law</ce:surname><ce:given-name>VG</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Antigenic variants of influenza A2 virus</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Bulletin of World Health Organization</sb:maintitle></sb:title><sb:volume-nr>31</sb:volume-nr></sb:series><sb:date>1964</sb:date></sb:issue><sb:pages><sb:first-page>129</sb:first-page><sb:last-page>132</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib5"><ce:label>5</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Natali</ce:surname><ce:given-name>A</ce:given-name></sb:author><sb:author><ce:surname>Oxford</ce:surname><ce:given-name>JS</ce:given-name></sb:author><sb:author><ce:surname>Schild</ce:surname><ce:given-name>GC</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Frequency of naturally occurring antibody to influenza virus antigenic variatns selected ‘<ce:italic>in vitro</ce:italic>’ with monoclonal antibody</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J Hyg Cam</sb:maintitle></sb:title><sb:volume-nr>87</sb:volume-nr></sb:series><sb:date>1981</sb:date></sb:issue><sb:pages><sb:first-page>185</sb:first-page><sb:last-page>190</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib6"><ce:label>6</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Stuart-Harris</ce:surname><ce:given-name>CH</ce:given-name></sb:author><sb:author><ce:surname>Schild</ce:surname><ce:given-name>GC</ce:given-name></sb:author><sb:author><ce:surname>Oxford</ce:surname><ce:given-name>JS</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Influenza, the Virus and the Disease</sb:maintitle></sb:title></sb:contribution><sb:host><sb:book><sb:date>1984</sb:date><sb:publisher><sb:name>Edward Arnold</sb:name><sb:location>Villanova, Pa</sb:location></sb:publisher></sb:book></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib7"><ce:label>7</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yamada</ce:surname><ce:given-name>A</ce:given-name></sb:author><sb:author><ce:surname>Brown</ce:surname><ce:given-name>LE</ce:given-name></sb:author><sb:author><ce:surname>Webster</ce:surname><ce:given-name>RC</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Characterisation of H2 influenza virus hemagglutinin with monoclonal antibodies: influence of receptor sepcificity</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Virology</sb:maintitle></sb:title><sb:volume-nr>138</sb:volume-nr></sb:series><sb:date>1984</sb:date></sb:issue><sb:pages><sb:first-page>276</sb:first-page><sb:last-page>286</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib8"><ce:label>8</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Davenpoort</ce:surname><ce:given-name>FM</ce:given-name></sb:author><sb:author><ce:surname>Hennessy</ce:surname><ce:given-name>AV</ce:given-name></sb:author><sb:author><ce:surname>Francis</ce:surname><ce:given-name>T</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Epidemiologic and immunologic significance of age distribution of antibody to antigenic variants of influenza virus</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J Exp Med</sb:maintitle></sb:title><sb:volume-nr>98</sb:volume-nr></sb:series><sb:date>1953</sb:date></sb:issue><sb:pages><sb:first-page>641</sb:first-page><sb:last-page>656</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib9"><ce:label>9</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Marine</ce:surname><ce:given-name>WM</ce:given-name></sb:author><sb:author><ce:surname>Workman</ce:surname><ce:given-name>WM</ce:given-name></sb:author><sb:author><ce:surname>Webster</ce:surname><ce:given-name>RG</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Immunological interrelationship of Hong Kong, Asian and Equi-2 influenza viruses in man</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Bulletin of World Health Organization</sb:maintitle></sb:title><sb:volume-nr>41</sb:volume-nr></sb:series><sb:date>1969</sb:date></sb:issue><sb:pages><sb:first-page>475</sb:first-page><sb:last-page>482</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib10"><ce:label>10</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Golubev</ce:surname><ce:given-name>DB</ce:given-name></sb:author><sb:author><ce:surname>Karpukhin</ce:surname><ce:given-name>GL</ce:given-name></sb:author><sb:author><ce:surname>Galitarov</ce:surname><ce:given-name>SS</ce:given-name></sb:author><sb:author><ce:surname>Denisov</ce:surname><ce:given-name>GM</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Type A influenze (H2N2) viruses isolated in Leningrad in 1980</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J Hyg Camb</sb:maintitle></sb:title><sb:volume-nr>95</sb:volume-nr></sb:series><sb:date>1985</sb:date></sb:issue><sb:pages><sb:first-page>493</sb:first-page><sb:last-page>504</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib11"><ce:label>11</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Schild</ce:surname><ce:given-name>GC</ce:given-name></sb:author><sb:author><ce:surname>Pereira</ce:surname><ce:given-name>MS</ce:given-name></sb:author><sb:author><ce:surname>Chakraverty</ce:surname><ce:given-name>P</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Single-radial-haemolysis: a new method for the assay of antibody to influenza haemagglutinin</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Bulletin of World Health Organization</sb:maintitle></sb:title><sb:volume-nr>52</sb:volume-nr></sb:series><sb:date>1975</sb:date></sb:issue><sb:pages><sb:first-page>43</sb:first-page><sb:last-page>50</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib12"><ce:label>12</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Mulder</ce:surname><ce:given-name>J</ce:given-name></sb:author><sb:author><ce:surname>Masurel</ce:surname><ce:given-name>N</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Pre-epidemic antibody against 1957 strain of asiatic influenza</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Lancet</sb:maintitle></sb:title><sb:volume-nr>1</sb:volume-nr></sb:series><sb:date>1958</sb:date></sb:issue><sb:pages><sb:first-page>810</sb:first-page><sb:last-page>814</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib13"><ce:label>13</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Hope-Simpson</ce:surname><ce:given-name>E</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Transmission of Epidemic Influenza</sb:maintitle></sb:title></sb:contribution><sb:host><sb:book><sb:date>1992</sb:date><sb:publisher><sb:name>Plenum</sb:name><sb:location>London</sb:location></sb:publisher></sb:book></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib14"><ce:label>14</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Kilbourne</ce:surname><ce:given-name>ED</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The Influenza Viruses and Influenza</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:date>1975</sb:date><sb:publisher><sb:name>Academic Press</sb:name><sb:location>N.Y.</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>309</sb:first-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib15"><ce:label>15</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Pyhala</ce:surname><ce:given-name>R</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Antibody status to influenza A/Singapore/1/57 (H2N2) in Finland during a period of outbreaks caused by H3N2 and H1N1 subtype viruses</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J Hyg Camb</sb:maintitle></sb:title><sb:volume-nr>95</sb:volume-nr></sb:series><sb:date>1985</sb:date></sb:issue><sb:pages><sb:first-page>437</sb:first-page><sb:last-page>445</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib16"><ce:label>16</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lin</ce:surname><ce:given-name>YP</ce:given-name></sb:author><sb:author><ce:surname>Shu</ce:surname><ce:given-name>LL</ce:given-name></sb:author><sb:author><ce:surname>Bean</ce:surname><ce:given-name>WJ</ce:given-name></sb:author><sb:author><ce:surname>Sharp</ce:surname><ce:given-name>GB</ce:given-name></sb:author><sb:author><ce:surname>Shortridge</ce:surname><ce:given-name>KF</ce:given-name></sb:author><sb:et-al></sb:et-al></sb:authors><sb:title><sb:maintitle>Analysis of the influenza virus gene pool of avian species from southern China</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Virology</sb:maintitle></sb:title><sb:volume-nr>198</sb:volume-nr></sb:series><sb:date>1994</sb:date></sb:issue><sb:pages><sb:first-page>557</sb:first-page><sb:last-page>566</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib17"><ce:label>17</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Webster</ce:surname><ce:given-name>RG</ce:given-name></sb:author><sb:author><ce:surname>Bean</ce:surname><ce:given-name>WJ</ce:given-name></sb:author><sb:author><ce:surname>Gorman</ce:surname><ce:given-name>OT</ce:given-name></sb:author><sb:author><ce:surname>Chambers</ce:surname><ce:given-name>TM</ce:given-name></sb:author><sb:author><ce:surname>Kawaoka</ce:surname><ce:given-name>Y</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Evolution of ecology of influenza A virus</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Microbiol Rev</sb:maintitle></sb:title><sb:volume-nr>56</sb:volume-nr></sb:series><sb:date>1992</sb:date></sb:issue><sb:pages><sb:first-page>152</sb:first-page><sb:last-page>179</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib18"><ce:label>18</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Patera</ce:surname><ce:given-name>AC</ce:given-name></sb:author><sb:author><ce:surname>Graham</ce:surname><ce:given-name>CM</ce:given-name></sb:author><sb:author><ce:surname>Thomas</ce:surname><ce:given-name>DB</ce:given-name></sb:author><sb:author><ce:surname>Smith</ce:surname><ce:given-name>CA</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Immunodominance with progenitor B cell diversity in the neutralizing antibody repertoire to influenza infection</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Eur J Immunol</sb:maintitle></sb:title><sb:volume-nr>25</sb:volume-nr></sb:series><sb:date>1995</sb:date></sb:issue><sb:pages><sb:first-page>623</sb:first-page><sb:last-page>628</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib19"><ce:label>19</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Natali</ce:surname><ce:given-name>A</ce:given-name></sb:author><sb:author><ce:surname>Chezzi</ce:surname><ce:given-name>C</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Use of monocloonal anti-haemagglutinin antibodies for the <ce:italic>in vitro</ce:italic> selection of a sequential infuenza virus antigenic variant</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Eur J Epidem</sb:maintitle></sb:title><sb:volume-nr>7</sb:volume-nr></sb:series><sb:date>1991</sb:date></sb:issue><sb:pages><sb:first-page>384</sb:first-page><sb:last-page>388</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib20"><ce:label>20</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Yewdell</ce:surname><ce:given-name>JW</ce:given-name></sb:author><sb:author><ce:surname>Catou</ce:surname><ce:given-name>AJ</ce:given-name></sb:author><sb:author><ce:surname>Gerhard</ce:surname><ce:given-name>W</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Selection of influenza A adsorptive mutants by growth in the presence of a mixture of monoclonal anti-haemagglutinin antibodies</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J Virol</sb:maintitle></sb:title><sb:volume-nr>57</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>623</sb:first-page><sb:last-page>628</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Natural and ‘ In vitro ’ selected antigenic variants of influenza A virus (H2N2)</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Natural and ‘</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Natali</namePart><affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Pilotti</namePart><affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.O.</namePart><namePart type="family">Valcavi</namePart><affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Chezzi</namePart><affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.S.</namePart><namePart type="family">Oxford</namePart><affiliation>Academic Virology, Department of Medical Microbilogy, St Bartholomew's and the Royal London School of Medicine, Turner Street, London E1 2AD, U.K.</affiliation><affiliation>Please address all correspondence to: J. S. Oxford.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">We provide data on the prevalence of SRH antibody to influenza A/Singapore/1/57 (H2N2). Approximately 10.3% of sera had antibody to the influenza A (H2N2) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H3N2) and 31.5% to influenza A (H2N1) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H2N2) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H2N2) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in vitro variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.</abstract><note type="content">Section title: Original article</note><relatedItem type="host"><titleInfo><title>Journal of Infection</title></titleInfo><titleInfo type="abbreviated"><title>YJINF</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued></originInfo><identifier type="ISSN">0163-4453</identifier><identifier type="PII">S0163-4453(00)X0120-X</identifier><part><date>1998</date><detail type="volume"><number>37</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>97</end></extent><extent unit="pages"><start>19</start><end>23</end></extent></part></relatedItem><identifier type="istex">CAB58BDF7560E54BB40F3369FCD10F8C9667E297</identifier><identifier type="ark">ark:/67375/6H6-VM1V2P47-B</identifier><identifier type="DOI">10.1016/S0163-4453(98)90260-2</identifier><identifier type="PII">S0163-4453(98)90260-2</identifier><identifier type="ArticleID">98902602</identifier><accessCondition type="use and reproduction" contentType="copyright">©1998 The British Infection Society</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>The British Infection Society, ©1998</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-VM1V2P47-B/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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