Natural and ‘ In vitro ’ selected antigenic variants of influenza A virus (H2N2)
Identifieur interne : 000014 ( Istex/Corpus ); précédent : 000013; suivant : 000015Natural and ‘ In vitro ’ selected antigenic variants of influenza A virus (H2N2)
Auteurs : A. Natali ; E. Pilotti ; P. O. Valcavi ; C. Chezzi ; J. S. OxfordSource :
- Journal of Infection [ 0163-4453 ] ; 1998.
English descriptors
- Teeft :
- Antibody, Antigenic, Antigenic site, Antigenic sites, Antigenic variant, Antigenic variants, Antigenic variation, Epitope, Haemolysis, Haemolysis area, Influenza, Influenza infection, Influenza virus, Influenza virus hemagglutinin, Influenza viruses, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Monoclonal antibody pressure, Natural variant, Natural variants, Original antigenic, Pandemic influenza, Parental, Parental virus, Positive sera, Previous studies, Serological, Serological analysis, Serological reactivity, Single epitope, Unilateral test, Variant, Virus, World health organization.
Abstract
We provide data on the prevalence of SRH antibody to influenza A/Singapore/1/57 (H2N2). Approximately 10.3% of sera had antibody to the influenza A (H2N2) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H3N2) and 31.5% to influenza A (H2N1) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H2N2) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H2N2) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in vitro variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.
Url:
DOI: 10.1016/S0163-4453(98)90260-2
Links to Exploration step
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<ce:author-group><ce:author><ce:given-name>A.</ce:given-name>
<ce:surname>Natali</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>E.</ce:given-name>
<ce:surname>Pilotti</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>P.O.</ce:given-name>
<ce:surname>Valcavi</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>C.</ce:given-name>
<ce:surname>Chezzi</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>J.S.</ce:given-name>
<ce:surname>Oxford</ce:surname>
<ce:cross-ref refid="aff2"><ce:sup>2</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="cor1"><ce:sup>*</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:affiliation id="aff1"><ce:label>1</ce:label>
<ce:textfn>Institute of Microbiology, University of Parma, Parma 43100, Italy</ce:textfn>
</ce:affiliation>
<ce:affiliation id="aff2"><ce:label>2</ce:label>
<ce:textfn>Academic Virology, Department of Medical Microbilogy, St Bartholomew's and the Royal London School of Medicine, Turner Street, London E1 2AD, U.K.</ce:textfn>
</ce:affiliation>
<ce:correspondence id="cor1"><ce:label>*</ce:label>
<ce:text>Please address all correspondence to: J. S. Oxford.</ce:text>
</ce:correspondence>
</ce:author-group>
<ce:date-accepted day="12" month="11" year="1997"></ce:date-accepted>
<ce:abstract id="ab1" class="author" xml:lang="en"><ce:abstract-sec><ce:simple-para>We provide data on the prevalence of SRH antibody to influenza A/Singapore/1/57 (H<ce:inf loc="post">2</ce:inf>
N<ce:inf loc="post">2</ce:inf>
). Approximately 10.3% of sera had antibody to the influenza A (H<ce:inf loc="post">2</ce:inf>
N<ce:inf loc="post">2</ce:inf>
) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H<ce:inf loc="post">3</ce:inf>
N<ce:inf loc="post">2</ce:inf>
) and 31.5% to influenza A (H<ce:inf loc="post">2</ce:inf>
N<ce:inf loc="post">1</ce:inf>
) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H<ce:inf loc="post">2</ce:inf>
N<ce:inf loc="post">2</ce:inf>
) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H<ce:inf loc="post">2</ce:inf>
N<ce:inf loc="post">2</ce:inf>
) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in <ce:italic>vitro</ce:italic>
variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
</head>
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<mods version="3.6"><titleInfo lang="en"><title>Natural and ‘ In vitro ’ selected antigenic variants of influenza A virus (H2N2)</title>
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<name type="personal"><namePart type="given">A.</namePart>
<namePart type="family">Natali</namePart>
<affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation>
<role><roleTerm type="text">author</roleTerm>
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</name>
<name type="personal"><namePart type="given">E.</namePart>
<namePart type="family">Pilotti</namePart>
<affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation>
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<name type="personal"><namePart type="given">P.O.</namePart>
<namePart type="family">Valcavi</namePart>
<affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation>
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<name type="personal"><namePart type="given">C.</namePart>
<namePart type="family">Chezzi</namePart>
<affiliation>Institute of Microbiology, University of Parma, Parma 43100, Italy</affiliation>
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<name type="personal"><namePart type="given">J.S.</namePart>
<namePart type="family">Oxford</namePart>
<affiliation>Academic Virology, Department of Medical Microbilogy, St Bartholomew's and the Royal London School of Medicine, Turner Street, London E1 2AD, U.K.</affiliation>
<affiliation>Please address all correspondence to: J. S. Oxford.</affiliation>
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<abstract lang="en">We provide data on the prevalence of SRH antibody to influenza A/Singapore/1/57 (H2N2). Approximately 10.3% of sera had antibody to the influenza A (H2N2) subtype virus in comparison to the 36.90% of positive sera to a representative influenza A (H3N2) and 31.5% to influenza A (H2N1) viruses. The percentage of subjects with antibody constantly decreased from the older to the younger age groups. Persons born after 1968 were essentially seronegative, whereas subjects born before 1900, and in the decade 1950–1959, showed the highest antibody levels to influenza A (H2N2) viruses. These age groups also appared to have ‘protective’ levels of anti-HA antibody to influenza A (H2N2) virus. An antigenic variant of A/Singapore/1/57 virus was selected in the laboratory using a monoclonal antibody to HA. Serological comparison of the new in vitro variant with the parental virus and two naturally occurring viruses, namely A/England/12/64 and Tokyo/3/67, showed that certain human sera were able to distinguish the variant, indicating a restricted antibody repertoire in these adult and children's sera, providing an explanation of how such variants could actually arise in nature.</abstract>
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