Study of molecular mechanisms of Influenza Virus genetic reassortment
Identifieur interne : 000272 ( Hal/Corpus ); précédent : 000271; suivant : 000273Study of molecular mechanisms of Influenza Virus genetic reassortment
Auteurs : Boris EssereSource :
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Abstract
The Flu is a frequent viral infectious disease caused by the Influenza viruses. Their genomes are composed by eight negative single-stranded RNA organised as vRNPs. During the viral cycle, the terminal non-coding and coding regions of viral genome have been shown to be crucial for the selective incorporation of a complete set of the eight vRNPs into influenza viral particles. Band shift assay and electron tomography allowed us to show that all gene segments interact together by RNA/RNA interactions involving their packaging region. Our results suggest that the eight genomic vRNAs are selected and packaged as an organized supramolecular complex held together between identified packaging regions into neosynthesized virions. Due to genome segmented nature, genetic reassortment can occur during co-infection. In order to identify molecular mechanisms responsible for the observed restriction during the genetic reassortment, we have developed a new competitive reverse genetic strategy allowing us to evaluate the genetic reassortment between H3N2 and H5N2 viruses. Our results suggest that mechanism controlling the packaging should regulate genetic reassortment. We have shown that the modulation of RNA/RNA interaction between HA and M gene segment have allowed us to increase HA H5 gene segment incorporation rate into a viral human genetic background, prerequisite for pandemic virus emergency
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Study of molecular mechanisms of Influenza Virus genetic reassortment</title><title xml:lang="fr">Étude des mécanismes moléculaires gouvernant le réassortiment génétique des virus Influenza de type A</title><author><name sortKey="Essere, Boris" sort="Essere, Boris" uniqKey="Essere B" first="Boris" last="Essere">Boris Essere</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-508819" status="OLD"> <idno type="RNSR">201119499B</idno> <orgName>Virologie et pathologie humaine</orgName> <orgName type="acronym">VirPath</orgName> <date type="start">2011-01-01</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation name="EA 4610" active="#struct-194495" type="direct"></relation> <relation active="#struct-301088" type="indirect"></relation> </listRelation> <tutelles><tutelle name="EA 4610" active="#struct-194495" type="direct"><org type="institution" xml:id="struct-194495" status="VALID"> <idno type="IdRef">026402823</idno> <idno type="ISNI">0000000121686185</idno> <orgName>Université Claude Bernard Lyon 1</orgName> <orgName type="acronym">UCBL</orgName> <desc> <address> <addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-lyon1.fr/</ref> </desc> <listRelation> <relation active="#struct-301088" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301088" type="indirect"><org type="regroupinstitution" xml:id="struct-301088" status="VALID"> <orgName>Université de Lyon</orgName> <desc> <address> <addrLine>92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.universite-lyon.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:tel-00867756</idno><idno type="halId">tel-00867756</idno><idno type="halUri">https://tel.archives-ouvertes.fr/tel-00867756</idno><idno type="url">https://tel.archives-ouvertes.fr/tel-00867756</idno><date when="2011-06-16">2011-06-16</date><idno type="wicri:Area/Hal/Corpus">000272</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Study of molecular mechanisms of Influenza Virus genetic reassortment</title><title xml:lang="fr">Étude des mécanismes moléculaires gouvernant le réassortiment génétique des virus Influenza de type A</title><author><name sortKey="Essere, Boris" sort="Essere, Boris" uniqKey="Essere B" first="Boris" last="Essere">Boris Essere</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-508819" status="OLD"> <idno type="RNSR">201119499B</idno> <orgName>Virologie et pathologie humaine</orgName> <orgName type="acronym">VirPath</orgName> <date type="start">2011-01-01</date> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation name="EA 4610" active="#struct-194495" type="direct"></relation> <relation active="#struct-301088" type="indirect"></relation> </listRelation> <tutelles><tutelle name="EA 4610" active="#struct-194495" type="direct"><org type="institution" xml:id="struct-194495" status="VALID"> <idno type="IdRef">026402823</idno> <idno type="ISNI">0000000121686185</idno> <orgName>Université Claude Bernard Lyon 1</orgName> <orgName type="acronym">UCBL</orgName> <desc> <address> <addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-lyon1.fr/</ref> </desc> <listRelation> <relation active="#struct-301088" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301088" type="indirect"><org type="regroupinstitution" xml:id="struct-301088" status="VALID"> <orgName>Université de Lyon</orgName> <desc> <address> <addrLine>92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.universite-lyon.fr/</ref> </desc> </org></tutelle></tutelles></hal:affiliation></affiliation></author></analytic></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Avian virus</term><term>Bundling</term><term>Genetic reassortment</term><term>Influenza A virus</term><term>RNA/RNA interaction</term></keywords><keywords scheme="mix" xml:lang="fr"><term>Empaquetage</term><term>Interaction ARN/ARN</term><term>Réassortiment génétique</term><term>Virus Influenza de type A</term><term>Virus aviaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>The Flu is a frequent viral infectious disease caused by the Influenza viruses. Their genomes are composed by eight negative single-stranded RNA organised as vRNPs. During the viral cycle, the terminal non-coding and coding regions of viral genome have been shown to be crucial for the selective incorporation of a complete set of the eight vRNPs into influenza viral particles. Band shift assay and electron tomography allowed us to show that all gene segments interact together by RNA/RNA interactions involving their packaging region. Our results suggest that the eight genomic vRNAs are selected and packaged as an organized supramolecular complex held together between identified packaging regions into neosynthesized virions. Due to genome segmented nature, genetic reassortment can occur during co-infection. In order to identify molecular mechanisms responsible for the observed restriction during the genetic reassortment, we have developed a new competitive reverse genetic strategy allowing us to evaluate the genetic reassortment between H3N2 and H5N2 viruses. Our results suggest that mechanism controlling the packaging should regulate genetic reassortment. We have shown that the modulation of RNA/RNA interaction between HA and M gene segment have allowed us to increase HA H5 gene segment incorporation rate into a viral human genetic background, prerequisite for pandemic virus emergency</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Study of molecular mechanisms of Influenza Virus genetic reassortment</title> <title xml:lang="fr">Étude des mécanismes moléculaires gouvernant le réassortiment génétique des virus Influenza de type A</title> <author role="aut"> <persName> <forename type="first">Boris</forename> <surname>Essere</surname> </persName> <idno type="halauthorid">781143</idno> <affiliation ref="#struct-508819"></affiliation> </author> <editor role="depositor"> <persName> <forename>ABES</forename> <surname>STAR</surname> </persName> <email type="md5">f5aa7f563b02bb6adbba7496989af39a</email> <email type="domain">abes.fr</email> </editor> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2013-09-30 14:53:07</date> <date type="whenModified">2019-11-20 03:04:26</date> <date type="whenReleased">2013-10-01 13:56:22</date> <date type="whenProduced">2011-06-16</date> <date type="whenEndEmbargoed">2013-09-30</date> <ref type="file" target="https://tel.archives-ouvertes.fr/tel-00867756/document"> <date notBefore="2013-09-30"></date> </ref> <ref type="file" subtype="author" n="1" target="https://tel.archives-ouvertes.fr/tel-00867756/file/TH2011_Essere_Boris.pdf"> <date notBefore="2013-09-30"></date> </ref> </edition> <respStmt> <resp>contributor</resp> <name key="131274"> <persName> <forename>ABES</forename> <surname>STAR</surname> </persName> <email type="md5">f5aa7f563b02bb6adbba7496989af39a</email> <email type="domain">abes.fr</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">tel-00867756</idno> <idno type="halUri">https://tel.archives-ouvertes.fr/tel-00867756</idno> <idno type="halBibtex">essere:tel-00867756</idno> <idno type="halRefHtml">Sciences agricoles. Université Claude Bernard - Lyon I, 2011. Français. ⟨NNT : 2011LYO10119⟩</idno> <idno type="halRef">Sciences agricoles. Université Claude Bernard - Lyon I, 2011. Français. ⟨NNT : 2011LYO10119⟩</idno> </publicationStmt> <seriesStmt> <idno type="stamp" n="STAR">STAR - Dépôt national des thèses électroniques</idno> <idno type="stamp" n="UNIV-LYON1">Université Claude Bernard - Lyon I</idno> <idno type="stamp" n="THESES_LYON1" corresp="UNIV-LYON1">Thèses Université Claude Bernard Lyon 1</idno> <idno type="stamp" n="UDL">UDL</idno> <idno type="stamp" n="UNIV-LYON">Université de Lyon</idno> </seriesStmt> <notesStmt></notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Study of molecular mechanisms of Influenza Virus genetic reassortment</title> <title xml:lang="fr">Étude des mécanismes moléculaires gouvernant le réassortiment génétique des virus Influenza de type A</title> <author role="aut"> <persName> <forename type="first">Boris</forename> <surname>Essere</surname> </persName> <idno type="halauthorid">781143</idno> <affiliation ref="#struct-508819"></affiliation> </author> </analytic> <monogr> <idno type="nnt">2011LYO10119</idno> <imprint> <date type="dateDefended">2011-06-16</date> </imprint> <authority type="institution">Université Claude Bernard - Lyon I</authority> <authority type="school">École Doctorale Evolution Ecosystèmes Microbiologie Modélisation</authority> <authority type="supervisor">Bruno Lina</authority> <authority type="supervisor">Vincent Moulès</authority> <authority type="jury">Sylvie Van der Werf [Rapporteur]</authority> <authority type="jury">Gaëlle Kuntz-Simon [Rapporteur]</authority> <authority type="jury">Jean-Claude Desenclos</authority> <authority type="jury">Isabelle Legastelois</authority> </monogr> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="fr">French</language> </langUsage> <textClass> <keywords scheme="author"> <term xml:lang="en">RNA/RNA interaction</term> <term xml:lang="en">Bundling</term> <term xml:lang="en">Genetic reassortment</term> <term xml:lang="en">Avian virus</term> <term xml:lang="en">Influenza A virus</term> <term xml:lang="fr">Virus Influenza de type A</term> <term xml:lang="fr">Virus aviaire</term> <term xml:lang="fr">Réassortiment génétique</term> <term xml:lang="fr">Empaquetage</term> <term xml:lang="fr">Interaction ARN/ARN</term> </keywords> <classCode scheme="halDomain" n="sdv.sa">Life Sciences [q-bio]/Agricultural sciences</classCode> <classCode scheme="halTypology" n="THESE">Theses</classCode> </textClass> <abstract xml:lang="en"> <p>The Flu is a frequent viral infectious disease caused by the Influenza viruses. Their genomes are composed by eight negative single-stranded RNA organised as vRNPs. During the viral cycle, the terminal non-coding and coding regions of viral genome have been shown to be crucial for the selective incorporation of a complete set of the eight vRNPs into influenza viral particles. Band shift assay and electron tomography allowed us to show that all gene segments interact together by RNA/RNA interactions involving their packaging region. Our results suggest that the eight genomic vRNAs are selected and packaged as an organized supramolecular complex held together between identified packaging regions into neosynthesized virions. Due to genome segmented nature, genetic reassortment can occur during co-infection. In order to identify molecular mechanisms responsible for the observed restriction during the genetic reassortment, we have developed a new competitive reverse genetic strategy allowing us to evaluate the genetic reassortment between H3N2 and H5N2 viruses. Our results suggest that mechanism controlling the packaging should regulate genetic reassortment. We have shown that the modulation of RNA/RNA interaction between HA and M gene segment have allowed us to increase HA H5 gene segment incorporation rate into a viral human genetic background, prerequisite for pandemic virus emergency</p> </abstract> <abstract xml:lang="fr"> <p>La grippe, infection respiratoire virale fréquente, est due aux virus Influenza. Leur génome est constitué par huit molécules d’ARN de polarité négative retrouvés sous la forme de complexe ribonucléique (RNPv). Au cours du cycle viral, il a été démontré que les régions terminales des segments de gène étaient cruciales pour l’incorporation sélective des huit RNPv à l’intérieur des particules virales. Par des techniques d’interaction in vitro et de tomographie électronique, nous avons montré que les segments de gène du virus H3N2 interagissaient entre eux par des interactions ARN/ARN impliquant leurs régions de packaging. Nos résultats suggèrent que la mise en place de ce réseau permettrait la formation d’un complexe supra macromoléculaire multi-segmenté permettant l’incorporation d’un jeu complet des huit RNPv dans les particules virales néosynthetisées. En raison de la nature segmentée du génome viral, des phénomènes de réassortiment génétique peuvent avoir lieu lors d’une co-infection. Afin de définir les mécanismes responsables de la restriction observée lors de ce phénomène, nous avons évalué le taux de réassortiment génétique in vitro entre le virus humain H3N2 et le virus aviaire H5N2. Nos résultats suggèrent que le mécanisme gouvernant l’incorporation sélective des segments de gènes, régulerait le réassortiment génétique. Nous avons montré que la modulation de l’interaction ARN/ARN entre les segments de gènes HA et M permet d’augmenter le taux d’incorporation du segment de gène HA H5 dans le fond génétique du virus humain, prérequis pour l’émergence de virus pandémique</p> </abstract> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
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