Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.
Identifieur interne : 000172 ( Hal/Checkpoint ); précédent : 000171; suivant : 000173Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.
Auteurs : Wafa Tombari [Tunisie] ; Mathilde Paul [France] ; Jihene Bettaieb [Tunisie] ; Imen Larbi [Tunisie] ; Jihene Nsiri [Tunisie] ; Imen Elbehi [Tunisie] ; Latifa Gribaa [Tunisie] ; Abdeljelil Ghram [Tunisie]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
OBJECTIVE: Estimate the seroprevalence of influenza A virus in various commercial poultry farms and evaluate specific risk factors as well as analyze their genetic nature using molecular assays. MATERIALS AND METHODS: This report summarizes the findings of a national survey realized from October 2010 to May 2011 on 800 flocks in 20 governorates. Serum samples were screened for the presence of specific influenza virus antibodies using cELISA test. Additionally, swab samples were tested by real time and conventional RT-PCR and compared with results obtained by others assays. Phylogenetic and genetic analyses of the glycoproteins were established for some strains. RESULTS: Out of the 800 chicken and turkey flocks tested by cELISA, 223 showed positive anti-NP antibodies (28.7%, 95% CI: 25.6-32.1). Significantly higher seroprevalence was found among the coastal areas compared to inland and during the autumn and winter. Broiler flocks showed significantly lower seroprevalence than layers and broiler breeders. The influenza virus infection prevalence increased after the laying phase among layer flocks. In addition, AIV seropositivity was significantly associated with low biosecurity measures. The Ag EIA and rRT-PCR tests revealed significantly higher numbers of AI positive samples as compared to cell cultures or egg inoculation. All new strains were subtyped as H9N2 by real time and conventional RT-PCR. Drift mutations, addition or deletion of glycosylation sites were likely to have occurred in the HA and NA glycoproteins of Tunisian strains resulting in multiple new amino acid substitutions. This fact may reflect different evolutionary pressures affecting these glycoproteins. The role of these newly detected substitutions should be tested. CONCLUSION: Our findings highlight the potential risk of AIV to avian health. Strict enforcement of biosecurity measures and possible vaccination of all poultry flocks with continuous monitoring of poultry stations may ensure reduction of AIV prevalence and avoid emergence of more pathogenic strains.
Url:
DOI: 10.1371/journal.pone.0053524
Links toward previous steps (curation, corpus...)
Links to Exploration step
Hal:pasteur-00859255Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.</title><author><name sortKey="Tombari, Wafa" sort="Tombari, Wafa" uniqKey="Tombari W" first="Wafa" last="Tombari">Wafa Tombari</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Paul, Mathilde" sort="Paul, Mathilde" uniqKey="Paul M" first="Mathilde" last="Paul">Mathilde Paul</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName> <orgName type="acronym">INRA</orgName> <date type="start">1946-05-18</date> <date type="end">2019-12-31</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.inra.fr</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Bettaieb, Jihene" sort="Bettaieb, Jihene" uniqKey="Bettaieb J" first="Jihene" last="Bettaieb">Jihene Bettaieb</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-163147" status="VALID"> <orgName>Laboratoire d'Entomologie Médicale [Tunis, Tunisie]</orgName> <desc> <address> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Larbi, Imen" sort="Larbi, Imen" uniqKey="Larbi I" first="Imen" last="Larbi">Imen Larbi</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Nsiri, Jihene" sort="Nsiri, Jihene" uniqKey="Nsiri J" first="Jihene" last="Nsiri">Jihene Nsiri</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Elbehi, Imen" sort="Elbehi, Imen" uniqKey="Elbehi I" first="Imen" last="Elbehi">Imen Elbehi</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Gribaa, Latifa" sort="Gribaa, Latifa" uniqKey="Gribaa L" first="Latifa" last="Gribaa">Latifa Gribaa</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Ghram, Abdeljelil" sort="Ghram, Abdeljelil" uniqKey="Ghram A" first="Abdeljelil" last="Ghram">Abdeljelil Ghram</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:pasteur-00859255</idno><idno type="halId">pasteur-00859255</idno><idno type="halUri">https://hal-riip.archives-ouvertes.fr/pasteur-00859255</idno><idno type="url">https://hal-riip.archives-ouvertes.fr/pasteur-00859255</idno><idno type="doi">10.1371/journal.pone.0053524</idno><date when="2013">2013</date><idno type="wicri:Area/Hal/Corpus">000255</idno><idno type="wicri:Area/Hal/Curation">000255</idno><idno type="wicri:Area/Hal/Checkpoint">000172</idno><idno type="wicri:explorRef" wicri:stream="Hal" wicri:step="Checkpoint">000172</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.</title><author><name sortKey="Tombari, Wafa" sort="Tombari, Wafa" uniqKey="Tombari W" first="Wafa" last="Tombari">Wafa Tombari</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Paul, Mathilde" sort="Paul, Mathilde" uniqKey="Paul M" first="Mathilde" last="Paul">Mathilde Paul</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-92114" status="OLD"> <orgName>Institut National de la Recherche Agronomique</orgName> <orgName type="acronym">INRA</orgName> <date type="start">1946-05-18</date> <date type="end">2019-12-31</date> <desc> <address> <country key="FR"></country> </address> <ref type="url">http://www.inra.fr</ref> </desc> </hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Bettaieb, Jihene" sort="Bettaieb, Jihene" uniqKey="Bettaieb J" first="Jihene" last="Bettaieb">Jihene Bettaieb</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-163147" status="VALID"> <orgName>Laboratoire d'Entomologie Médicale [Tunis, Tunisie]</orgName> <desc> <address> <country key="TN"></country> </address> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Larbi, Imen" sort="Larbi, Imen" uniqKey="Larbi I" first="Imen" last="Larbi">Imen Larbi</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Nsiri, Jihene" sort="Nsiri, Jihene" uniqKey="Nsiri J" first="Jihene" last="Nsiri">Jihene Nsiri</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Elbehi, Imen" sort="Elbehi, Imen" uniqKey="Elbehi I" first="Imen" last="Elbehi">Imen Elbehi</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Gribaa, Latifa" sort="Gribaa, Latifa" uniqKey="Gribaa L" first="Latifa" last="Gribaa">Latifa Gribaa</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Ghram, Abdeljelil" sort="Ghram, Abdeljelil" uniqKey="Ghram A" first="Abdeljelil" last="Ghram">Abdeljelil Ghram</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author></analytic><idno type="DOI">10.1371/journal.pone.0053524</idno><series><title level="j">PLoS ONE</title><idno type="ISSN">1932-6203</idno><imprint><date type="datePub">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>OBJECTIVE: Estimate the seroprevalence of influenza A virus in various commercial poultry farms and evaluate specific risk factors as well as analyze their genetic nature using molecular assays. MATERIALS AND METHODS: This report summarizes the findings of a national survey realized from October 2010 to May 2011 on 800 flocks in 20 governorates. Serum samples were screened for the presence of specific influenza virus antibodies using cELISA test. Additionally, swab samples were tested by real time and conventional RT-PCR and compared with results obtained by others assays. Phylogenetic and genetic analyses of the glycoproteins were established for some strains. RESULTS: Out of the 800 chicken and turkey flocks tested by cELISA, 223 showed positive anti-NP antibodies (28.7%, 95% CI: 25.6-32.1). Significantly higher seroprevalence was found among the coastal areas compared to inland and during the autumn and winter. Broiler flocks showed significantly lower seroprevalence than layers and broiler breeders. The influenza virus infection prevalence increased after the laying phase among layer flocks. In addition, AIV seropositivity was significantly associated with low biosecurity measures. The Ag EIA and rRT-PCR tests revealed significantly higher numbers of AI positive samples as compared to cell cultures or egg inoculation. All new strains were subtyped as H9N2 by real time and conventional RT-PCR. Drift mutations, addition or deletion of glycosylation sites were likely to have occurred in the HA and NA glycoproteins of Tunisian strains resulting in multiple new amino acid substitutions. This fact may reflect different evolutionary pressures affecting these glycoproteins. The role of these newly detected substitutions should be tested. CONCLUSION: Our findings highlight the potential risk of AIV to avian health. Strict enforcement of biosecurity measures and possible vaccination of all poultry flocks with continuous monitoring of poultry stations may ensure reduction of AIV prevalence and avoid emergence of more pathogenic strains.</p> </div></front></TEI><hal api="V3"> <titleStmt> <title xml:lang="en">Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.</title> <author role="aut"> <persName> <forename type="first">Wafa</forename> <surname>Tombari</surname> </persName> <idno type="halauthorid">764215</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Mathilde</forename> <surname>Paul</surname> </persName> <idno type="halauthorid">400120</idno> <affiliation ref="#struct-92114"></affiliation> <affiliation ref="#struct-300421"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jihene</forename> <surname>Bettaieb</surname> </persName> <idno type="halauthorid">763219</idno> <affiliation ref="#struct-163147"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Imen</forename> <surname>Larbi</surname> </persName> <idno type="halauthorid">623984</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jihene</forename> <surname>Nsiri</surname> </persName> <idno type="halauthorid">764216</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Imen</forename> <surname>Elbehi</surname> </persName> <idno type="halauthorid">885128</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Latifa</forename> <surname>Gribaa</surname> </persName> <idno type="halauthorid">885129</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="crp"> <persName> <forename type="first">Abdeljelil</forename> <surname>Ghram</surname> </persName> <email type="md5">8607f92a88bf6e43f64f05b5864d50f5</email> <email type="domain">pasteur.rns.tn</email> <idno type="halauthorid">825183</idno> <affiliation ref="#struct-300103"></affiliation> </author> <editor role="depositor"> <persName> <forename>Institut</forename> <surname>Pasteur Tunis</surname> </persName> <email type="md5">3efb30976f844a878562d94d749609ae</email> <email type="domain">pasteur.tn</email> </editor> <funder>This work has been developed under the MEV09 project and the research platform of Veterinary of Epidemiology and Microbiology Laboratory</funder> </titleStmt> <editionStmt> <edition n="v1" type="current"> <date type="whenSubmitted">2013-09-06 16:52:37</date> <date type="whenModified">2020-03-17 02:52:52</date> <date type="whenReleased">2014-01-14 11:51:04</date> <date type="whenProduced">2013</date> <date type="whenEndEmbargoed">2013-09-06</date> <ref type="file" target="https://hal-riip.archives-ouvertes.fr/pasteur-00859255/document"> <date notBefore="2013-09-06"></date> </ref> <ref type="file" subtype="greenPublisher" n="1" target="https://hal-riip.archives-ouvertes.fr/pasteur-00859255/file/journal.pone.0053524.pdf"> <date notBefore="2013-09-06"></date> </ref> <ref type="externalLink" target="https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0053524&type=printable"></ref> </edition> <respStmt> <resp>contributor</resp> <name key="161267"> <persName> <forename>Institut</forename> <surname>Pasteur Tunis</surname> </persName> <email type="md5">3efb30976f844a878562d94d749609ae</email> <email type="domain">pasteur.tn</email> </name> </respStmt> </editionStmt> <publicationStmt> <distributor>CCSD</distributor> <idno type="halId">pasteur-00859255</idno> <idno type="halUri">https://hal-riip.archives-ouvertes.fr/pasteur-00859255</idno> <idno type="halBibtex">tombari:pasteur-00859255</idno> <idno type="halRefHtml">PLoS ONE, Public Library of Science, 2013, 8 (1), pp.e53524. ⟨10.1371/journal.pone.0053524⟩</idno> <idno type="halRef">PLoS ONE, Public Library of Science, 2013, 8 (1), pp.e53524. ⟨10.1371/journal.pone.0053524⟩</idno> </publicationStmt> <seriesStmt> <idno type="stamp" n="RIIP">Institut Pasteur RIIP (Réseau International)</idno> <idno type="stamp" n="RIIP_TUNIS" corresp="RIIP">Institut Pasteur de Tunis</idno> <idno type="stamp" n="AGREENIUM">Archive ouverte en agrobiosciences</idno> <idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno> <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno> </seriesStmt> <notesStmt> <note type="audience" n="2">International</note> <note type="popular" n="0">No</note> <note type="peer" n="1">Yes</note> </notesStmt> <sourceDesc> <biblStruct> <analytic> <title xml:lang="en">Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.</title> <author role="aut"> <persName> <forename type="first">Wafa</forename> <surname>Tombari</surname> </persName> <idno type="halauthorid">764215</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Mathilde</forename> <surname>Paul</surname> </persName> <idno type="halauthorid">400120</idno> <affiliation ref="#struct-92114"></affiliation> <affiliation ref="#struct-300421"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jihene</forename> <surname>Bettaieb</surname> </persName> <idno type="halauthorid">763219</idno> <affiliation ref="#struct-163147"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Imen</forename> <surname>Larbi</surname> </persName> <idno type="halauthorid">623984</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Jihene</forename> <surname>Nsiri</surname> </persName> <idno type="halauthorid">764216</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Imen</forename> <surname>Elbehi</surname> </persName> <idno type="halauthorid">885128</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="aut"> <persName> <forename type="first">Latifa</forename> <surname>Gribaa</surname> </persName> <idno type="halauthorid">885129</idno> <affiliation ref="#struct-409923"></affiliation> </author> <author role="crp"> <persName> <forename type="first">Abdeljelil</forename> <surname>Ghram</surname> </persName> <email type="md5">8607f92a88bf6e43f64f05b5864d50f5</email> <email type="domain">pasteur.rns.tn</email> <idno type="halauthorid">825183</idno> <affiliation ref="#struct-300103"></affiliation> </author> </analytic> <monogr> <idno type="halJournalId" status="VALID">28700</idno> <idno type="issn">1932-6203</idno> <idno type="eissn">1932-6203</idno> <title level="j">PLoS ONE</title> <imprint> <publisher>Public Library of Science</publisher> <biblScope unit="volume">8</biblScope> <biblScope unit="issue">1</biblScope> <biblScope unit="pp">e53524</biblScope> <date type="datePub">2013</date> <date type="dateEpub">2013-01-11</date> </imprint> </monogr> <idno type="doi">10.1371/journal.pone.0053524</idno> <idno type="pubmed">23326449</idno> </biblStruct> </sourceDesc> <profileDesc> <langUsage> <language ident="en">English</language> </langUsage> <textClass> <classCode scheme="mesh">Animals</classCode> <classCode scheme="mesh">Logistic Models</classCode> <classCode scheme="mesh">Molecular Sequence Data</classCode> <classCode scheme="mesh">Multivariate Analysis</classCode> <classCode scheme="mesh">Orthomyxoviridae</classCode> <classCode scheme="mesh">Phylogeny</classCode> <classCode scheme="mesh">Poultry</classCode> <classCode scheme="mesh">Risk Factors</classCode> <classCode scheme="mesh">Seroepidemiologic Studies</classCode> <classCode scheme="mesh">Tunisia</classCode> <classCode scheme="mesh">Turkeys</classCode> <classCode scheme="mesh">Antibodies, Viral</classCode> <classCode scheme="mesh">Viral Proteins</classCode> <classCode scheme="mesh">Amino Acid Sequence</classCode> <classCode scheme="mesh">Chickens</classCode> <classCode scheme="mesh">Commerce</classCode> <classCode scheme="mesh">DNA, Viral</classCode> <classCode scheme="mesh">Enzyme-Linked Immunosorbent Assay</classCode> <classCode scheme="mesh">Genes, Viral</classCode> <classCode scheme="mesh">Geography</classCode> <classCode scheme="mesh">Influenza in Birds</classCode> <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode> <classCode scheme="halTypology" n="ART">Journal articles</classCode> </textClass> <abstract xml:lang="en"> <p>OBJECTIVE: Estimate the seroprevalence of influenza A virus in various commercial poultry farms and evaluate specific risk factors as well as analyze their genetic nature using molecular assays. MATERIALS AND METHODS: This report summarizes the findings of a national survey realized from October 2010 to May 2011 on 800 flocks in 20 governorates. Serum samples were screened for the presence of specific influenza virus antibodies using cELISA test. Additionally, swab samples were tested by real time and conventional RT-PCR and compared with results obtained by others assays. Phylogenetic and genetic analyses of the glycoproteins were established for some strains. RESULTS: Out of the 800 chicken and turkey flocks tested by cELISA, 223 showed positive anti-NP antibodies (28.7%, 95% CI: 25.6-32.1). Significantly higher seroprevalence was found among the coastal areas compared to inland and during the autumn and winter. Broiler flocks showed significantly lower seroprevalence than layers and broiler breeders. The influenza virus infection prevalence increased after the laying phase among layer flocks. In addition, AIV seropositivity was significantly associated with low biosecurity measures. The Ag EIA and rRT-PCR tests revealed significantly higher numbers of AI positive samples as compared to cell cultures or egg inoculation. All new strains were subtyped as H9N2 by real time and conventional RT-PCR. Drift mutations, addition or deletion of glycosylation sites were likely to have occurred in the HA and NA glycoproteins of Tunisian strains resulting in multiple new amino acid substitutions. This fact may reflect different evolutionary pressures affecting these glycoproteins. The role of these newly detected substitutions should be tested. CONCLUSION: Our findings highlight the potential risk of AIV to avian health. Strict enforcement of biosecurity measures and possible vaccination of all poultry flocks with continuous monitoring of poultry stations may ensure reduction of AIV prevalence and avoid emergence of more pathogenic strains.</p> </abstract> </profileDesc> </hal></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Hal/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000172 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Hal/Checkpoint/biblio.hfd -nk 000172 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Hal
|étape= Checkpoint
|type= RBID
|clé= Hal:pasteur-00859255
|texte= Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.
}}
| This area was generated with Dilib version V0.6.33. |  |