Functional definition of HLA-DR and -DQ epitopes specific for DR2-short, DwFJO haplotype
Identifieur interne : 000315 ( France/Analysis ); précédent : 000314; suivant : 000316Functional definition of HLA-DR and -DQ epitopes specific for DR2-short, DwFJO haplotype
Auteurs : D. Zeliszewski [France] ; G. Sterkers [France] ; C. Freidel [France] ; L. Gebuhrer [France] ; H. Betuel [France] ; J. P. Levy [France]Source :
- Immunogenetics [ 0093-7711 ] ; 1986-06-01.
English descriptors
- Teeft :
- Antigenic, Antigenic specificities, Assay, Autologous, Betuel, Biochemical studies, Broad class, Cell lines, Clone, Clone iiig, Culture medium, Determinant, Different epitopes, Different patterns, Dqwl molecules, Dwfjo, Dwfjo haplotype, Epitope, Freidel, Functional definition, Functional inhibition studies, Gebuhrer, Haplotype, Histocompatibility, Iiig, Immunol, Influenza, Influenza virus, Influenza viruses, Mabs, Monoclonal antibodies, Negative control, Other hand, Positive results, Proliferation assays, Reinsmoen, Specific antigen, Sterkers, Tissue antigens, Triplicate cultures, Ucla tissue typing laboratory, Virus strains, Zeliszewski.
Abstract
Abstract: T-lymphocyte clones specific for the influenza A/Texas virus were obtained by limiting dilution of activated T cells from an HLA A2/3, B7/39, Cw -/-, DR2-short/2 short, DQw1/w1, DwFJO/FJO donor. Among the proliferating clones studied, and irrespective of their antigenic specificities, most of them were restricted by epitope(s) on HLA-DR molecules present only on DR2-short/DwFJO cells but not on DR2-negative or DR2-long positive (Dw2, Dw12, Dw-) cells. Two clones were restricted by epitopes borne by DQ products. Here again, these epitopes were present on DR2-short/DwFJO but not on DR2-long, DQw1 (Dw2, Dw12) cells, indicating that the DQwl molecules of DR2-long and DR2-short haplotypes are different. Taken together, these results indicate that the DR2-short, DwFJO haplotype is characterized by both HLA-DR- and DQ-specific molecules. Finally, one clone was restricted by an epitope shared by DR products from DR2 short/DwFJO, DRw11, and DRw13 haplotypes. This latter functional determinant has never been described until now.
Url:
DOI: 10.1007/BF00372671
Affiliations:
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ISTEX:C1EE22A358B8C7AA84805C7E4C24B7BABE5CFCA0Le document en format XML
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Levy</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Immunologie et Virologie des Tumeurs, INSERM U 152, Hôpital Cochin, 27, rue du Faubourg Saint-Jacques, 75674, Paris Cédex 14</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris Cédex 14</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunogenetics</title><title level="j" type="abbrev">Immunogenetics</title><idno type="ISSN">0093-7711</idno><idno type="eISSN">1432-1211</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1986-06-01">1986-06-01</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="385">385</biblScope><biblScope unit="page" to="392">392</biblScope></imprint><idno type="ISSN">0093-7711</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0093-7711</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antigenic</term><term>Antigenic specificities</term><term>Assay</term><term>Autologous</term><term>Betuel</term><term>Biochemical studies</term><term>Broad class</term><term>Cell lines</term><term>Clone</term><term>Clone iiig</term><term>Culture medium</term><term>Determinant</term><term>Different epitopes</term><term>Different patterns</term><term>Dqwl molecules</term><term>Dwfjo</term><term>Dwfjo haplotype</term><term>Epitope</term><term>Freidel</term><term>Functional definition</term><term>Functional inhibition studies</term><term>Gebuhrer</term><term>Haplotype</term><term>Histocompatibility</term><term>Iiig</term><term>Immunol</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Mabs</term><term>Monoclonal antibodies</term><term>Negative control</term><term>Other hand</term><term>Positive results</term><term>Proliferation assays</term><term>Reinsmoen</term><term>Specific antigen</term><term>Sterkers</term><term>Tissue antigens</term><term>Triplicate cultures</term><term>Ucla tissue typing laboratory</term><term>Virus strains</term><term>Zeliszewski</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: T-lymphocyte clones specific for the influenza A/Texas virus were obtained by limiting dilution of activated T cells from an HLA A2/3, B7/39, Cw -/-, DR2-short/2 short, DQw1/w1, DwFJO/FJO donor. Among the proliferating clones studied, and irrespective of their antigenic specificities, most of them were restricted by epitope(s) on HLA-DR molecules present only on DR2-short/DwFJO cells but not on DR2-negative or DR2-long positive (Dw2, Dw12, Dw-) cells. Two clones were restricted by epitopes borne by DQ products. Here again, these epitopes were present on DR2-short/DwFJO but not on DR2-long, DQw1 (Dw2, Dw12) cells, indicating that the DQwl molecules of DR2-long and DR2-short haplotypes are different. Taken together, these results indicate that the DR2-short, DwFJO haplotype is characterized by both HLA-DR- and DQ-specific molecules. Finally, one clone was restricted by an epitope shared by DR products from DR2 short/DwFJO, DRw11, and DRw13 haplotypes. This latter functional determinant has never been described until now.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li><li>Île-de-France</li></region><settlement><li>Lyon Cédex 7</li><li>Paris Cédex 14</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Zeliszewski, D" sort="Zeliszewski, D" uniqKey="Zeliszewski D" first="D." last="Zeliszewski">D. Zeliszewski</name></region><name sortKey="Betuel, H" sort="Betuel, H" uniqKey="Betuel H" first="H." last="Betuel">H. Betuel</name><name sortKey="Freidel, C" sort="Freidel, C" uniqKey="Freidel C" first="C." last="Freidel">C. Freidel</name><name sortKey="Gebuhrer, L" sort="Gebuhrer, L" uniqKey="Gebuhrer L" first="L." last="Gebuhrer">L. Gebuhrer</name><name sortKey="Levy, J P" sort="Levy, J P" uniqKey="Levy J" first="J. P." last="Levy">J. P. Levy</name><name sortKey="Sterkers, G" sort="Sterkers, G" uniqKey="Sterkers G" first="G." last="Sterkers">G. Sterkers</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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