Serveur d'exploration sur la grippe aux Pays-Bas

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Measurement of antibodies to avian influenza virus A(H7N7) in humans by hemagglutination inhibition test.

Identifieur interne : 000338 ( Main/Exploration ); précédent : 000337; suivant : 000339

Measurement of antibodies to avian influenza virus A(H7N7) in humans by hemagglutination inhibition test.

Auteurs : Adam Meijer [Pays-Bas] ; Arnold Bosman ; Esther E H M. Van De Kamp ; Berry Wilbrink ; Mirna Du Ry Van Beest Holle ; Marion Koopmans

Source :

RBID : pubmed:16271401

Descripteurs français

English descriptors

Abstract

During the epizootic of highly pathogenic avian influenza A(H7N7) in 2003 in The Netherlands, RT-PCR and culture confirmed infection was detected in 89 persons who were ill. A modified hemagglutination inhibition (HI) test using horse erythrocytes and 2 hemagglutinating units of virus was applied to assess retrospectively the extent of human (subclinical) infection. Validation of the HI-test with sera from 34 RT-PCR and culture confirmed A(H7) infected persons and sera from 100 persons from a human influenza vaccine trial in autumn 2002 showed that this HI-test had a sensitivity of 85% and a specificity of 100% when using a cut-off titer of > or =10. Using this cut-off value, A(H7) specific antibodies were detected in 49% of 508 persons exposed to poultry and in 64% of 63 persons exposed to A(H7) infected persons. Correlation of seropositivity with the occurrence of eye symptoms in exposed persons who had not received antiviral prophylaxis and of reduced seropositivity with taking antiviral prophylaxis provided further evidence that the A(H7) HI antibody titers were real. In conclusion, by applying an HI-test using horse erythrocytes human antibodies against the avian A(H7N7) virus were detected with high sensitivity and specificity in an unexpectedly high proportion of exposed persons.

DOI: 10.1016/j.jviromet.2005.10.001
PubMed: 16271401


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<div type="abstract" xml:lang="en">During the epizootic of highly pathogenic avian influenza A(H7N7) in 2003 in The Netherlands, RT-PCR and culture confirmed infection was detected in 89 persons who were ill. A modified hemagglutination inhibition (HI) test using horse erythrocytes and 2 hemagglutinating units of virus was applied to assess retrospectively the extent of human (subclinical) infection. Validation of the HI-test with sera from 34 RT-PCR and culture confirmed A(H7) infected persons and sera from 100 persons from a human influenza vaccine trial in autumn 2002 showed that this HI-test had a sensitivity of 85% and a specificity of 100% when using a cut-off titer of > or =10. Using this cut-off value, A(H7) specific antibodies were detected in 49% of 508 persons exposed to poultry and in 64% of 63 persons exposed to A(H7) infected persons. Correlation of seropositivity with the occurrence of eye symptoms in exposed persons who had not received antiviral prophylaxis and of reduced seropositivity with taking antiviral prophylaxis provided further evidence that the A(H7) HI antibody titers were real. In conclusion, by applying an HI-test using horse erythrocytes human antibodies against the avian A(H7N7) virus were detected with high sensitivity and specificity in an unexpectedly high proportion of exposed persons.</div>
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<name sortKey="Bosman, Arnold" sort="Bosman, Arnold" uniqKey="Bosman A" first="Arnold" last="Bosman">Arnold Bosman</name>
<name sortKey="Du Ry Van Beest Holle, Mirna" sort="Du Ry Van Beest Holle, Mirna" uniqKey="Du Ry Van Beest Holle M" first="Mirna" last="Du Ry Van Beest Holle">Mirna Du Ry Van Beest Holle</name>
<name sortKey="Koopmans, Marion" sort="Koopmans, Marion" uniqKey="Koopmans M" first="Marion" last="Koopmans">Marion Koopmans</name>
<name sortKey="Van De Kamp, Esther E H M" sort="Van De Kamp, Esther E H M" uniqKey="Van De Kamp E" first="Esther E H M" last="Van De Kamp">Esther E H M. Van De Kamp</name>
<name sortKey="Wilbrink, Berry" sort="Wilbrink, Berry" uniqKey="Wilbrink B" first="Berry" last="Wilbrink">Berry Wilbrink</name>
</noCountry>
<country name="Pays-Bas">
<noRegion>
<name sortKey="Meijer, Adam" sort="Meijer, Adam" uniqKey="Meijer A" first="Adam" last="Meijer">Adam Meijer</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/GrippePaysBasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000338 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000338 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    GrippePaysBasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:16271401
   |texte=   Measurement of antibodies to avian influenza virus A(H7N7) in humans by hemagglutination inhibition test.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:16271401" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a GrippePaysBasV1 

Wicri

This area was generated with Dilib version V0.6.35.
Data generation: Mon Aug 10 10:18:30 2020. Site generation: Sat Mar 27 13:53:34 2021