GrippeCanadaV4, Main, Exploration, bibRecordById, pubmed:25416300

Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.

Identifieur interne : 000278 ( Main/Exploration ); précédent : 000277; suivant : 000279

Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.

Auteurs : Helena Grgi [Canada] ; Marcio Costa [Canada] ; Robert M. Friendship [Canada] ; Susy Carman [Canada] ; Éva Nagy [Canada] ; Greg Wideman [Canada] ; Scott Weese [Canada] ; Zvonimir Poljak [Canada]

Source :

Virology journal [ 1743-422X ] ; 2014.

RBID : pubmed:25416300

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Genetic Variation (MeSH), Genotype (MeSH), Influenza A Virus, H3N2 Subtype (classification), Influenza A Virus, H3N2 Subtype (genetics), Influenza A Virus, H3N2 Subtype (isolation & purification), Molecular Sequence Data (MeSH), Ontario (MeSH), Orthomyxoviridae Infections (veterinary), Orthomyxoviridae Infections (virology), Phylogeny (MeSH), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction (MeSH), Reassortant Viruses (classification), Reassortant Viruses (genetics), Reassortant Viruses (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH), Sequence Homology (MeSH), Swine (MeSH), Swine Diseases (virology).
MESH :
- chemical , genetics : RNA, Viral.
- geographic : Ontario.
- classification : Influenza A Virus, H3N2 Subtype, Reassortant Viruses.
- genetics : Influenza A Virus, H3N2 Subtype, Reassortant Viruses.
- isolation & purification : Influenza A Virus, H3N2 Subtype, Reassortant Viruses.
- veterinary : Orthomyxoviridae Infections.
- virology : Orthomyxoviridae Infections, Swine Diseases.
- Amino Acid Sequence, Animals, Genetic Variation, Genotype, Molecular Sequence Data, Phylogeny, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Swine.

Abstract

BACKGROUND

Data about molecular diversity of commonly circulating type A influenza viruses in Ontario swine are scarce. Yet, this information is essential for surveillance of animal and public health, vaccine updates, and for understanding virus evolution and its large-scale spread.

METHODS

The study population consisted of 21 swine herds with clinical problems due to respiratory disease. Nasal swabs from individual pigs were collected and tested by virus isolation in MDCK cells and by rtRT-PCR. All eight segments of 10 H3N2 viruses were sequenced using high-throughput sequencing and molecularly characterized.

RESULTS

Within-herd prevalence ranged between 2 and 100%. Structurally, Ontario H3N2 viruses could be classified into three different groups. Group 1 was the most similar to the original trH3N2 virus from 2005. Group 2 was the most similar to the Ontario turkey H3N2 isolates with PB1 and NS genes originating from trH3N2 virus and M, PB2, PA and NP genes originating from the A(H1N1)pdm09 virus. All Group 3 internal genes were genetically related to A(H1N1)pdm09. Analysis of antigenic sites of HA1 showed that Group 1 had 8 aa changes within 4 antigenic sites, A(1), B(3), C(2) and E(2). The Group 2 viruses had 8 aa changes within 3 antigenic sites A(3), B(3) and C(2), while Group 3 viruses had 4 aa changes within 3 antigenic sites, B(1), D(1) and E(2), when compared to the cluster IV H3N2 virus [A/swine/Ontario/33853/2005/(H3N2)].

CONCLUSIONS

The characterization of the Ontario H3N2 viruses clearly indicates reassortment of gene segments between the North American swine trH3N2 from cluster IV and the A(H1N1)pdm09 virus.

DOI: 10.1186/s12985-014-0194-z
PubMed: 25416300
PubMed Central: PMC4245826

Affiliations:

Canada

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000250
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Le document en format XML

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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Data about molecular diversity of commonly circulating type A influenza viruses in Ontario swine are scarce. Yet, this information is essential for surveillance of animal and public health, vaccine updates, and for understanding virus evolution and its large-scale spread.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>The study population consisted of 21 swine herds with clinical problems due to respiratory disease. Nasal swabs from individual pigs were collected and tested by virus isolation in MDCK cells and by rtRT-PCR. All eight segments of 10 H3N2 viruses were sequenced using high-throughput sequencing and molecularly characterized.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Within-herd prevalence ranged between 2 and 100%. Structurally, Ontario H3N2 viruses could be classified into three different groups. Group 1 was the most similar to the original trH3N2 virus from 2005. Group 2 was the most similar to the Ontario turkey H3N2 isolates with PB1 and NS genes originating from trH3N2 virus and M, PB2, PA and NP genes originating from the A(H1N1)pdm09 virus. All Group 3 internal genes were genetically related to A(H1N1)pdm09. Analysis of antigenic sites of HA1 showed that Group 1 had 8 aa changes within 4 antigenic sites, A(1), B(3), C(2) and E(2). The Group 2 viruses had 8 aa changes within 3 antigenic sites A(3), B(3) and C(2), while Group 3 viruses had 4 aa changes within 3 antigenic sites, B(1), D(1) and E(2), when compared to the cluster IV H3N2 virus [A/swine/Ontario/33853/2005/(H3N2)].</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The characterization of the Ontario H3N2 viruses clearly indicates reassortment of gene segments between the North American swine trH3N2 from cluster IV and the A(H1N1)pdm09 virus.</p>
</div>
</front>
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<DateCompleted><Year>2015</Year>
<Month>10</Month>
<Day>12</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1743-422X</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>11</Volume>
<PubDate><Year>2014</Year>
<Month>Nov</Month>
<Day>22</Day>
</PubDate>
</JournalIssue>
<Title>Virology journal</Title>
<ISOAbbreviation>Virol. J.</ISOAbbreviation>
</Journal>
<ArticleTitle>Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.</ArticleTitle>
<Pagination><MedlinePgn>194</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s12985-014-0194-z</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Data about molecular diversity of commonly circulating type A influenza viruses in Ontario swine are scarce. Yet, this information is essential for surveillance of animal and public health, vaccine updates, and for understanding virus evolution and its large-scale spread.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">The study population consisted of 21 swine herds with clinical problems due to respiratory disease. Nasal swabs from individual pigs were collected and tested by virus isolation in MDCK cells and by rtRT-PCR. All eight segments of 10 H3N2 viruses were sequenced using high-throughput sequencing and molecularly characterized.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Within-herd prevalence ranged between 2 and 100%. Structurally, Ontario H3N2 viruses could be classified into three different groups. Group 1 was the most similar to the original trH3N2 virus from 2005. Group 2 was the most similar to the Ontario turkey H3N2 isolates with PB1 and NS genes originating from trH3N2 virus and M, PB2, PA and NP genes originating from the A(H1N1)pdm09 virus. All Group 3 internal genes were genetically related to A(H1N1)pdm09. Analysis of antigenic sites of HA1 showed that Group 1 had 8 aa changes within 4 antigenic sites, A(1), B(3), C(2) and E(2). The Group 2 viruses had 8 aa changes within 3 antigenic sites A(3), B(3) and C(2), while Group 3 viruses had 4 aa changes within 3 antigenic sites, B(1), D(1) and E(2), when compared to the cluster IV H3N2 virus [A/swine/Ontario/33853/2005/(H3N2)].</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The characterization of the Ontario H3N2 viruses clearly indicates reassortment of gene segments between the North American swine trH3N2 from cluster IV and the A(H1N1)pdm09 virus.</AbstractText>
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<ForeName>Helena</ForeName>
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<AffiliationInfo><Affiliation>Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2 W1, Canada. hgrgic@uoguelph.ca.</Affiliation>
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<AffiliationInfo><Affiliation>Centre for Public Health and Zoonoses, University of Guelph, Guelph, Canada. hgrgic@uoguelph.ca.</Affiliation>
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<AffiliationInfo><Affiliation>Centre for Public Health and Zoonoses, University of Guelph, Guelph, Canada. costam@uoguelph.ca.</Affiliation>
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<AffiliationInfo><Affiliation>Centre for Public Health and Zoonoses, University of Guelph, Guelph, Canada. rfriends@uoguelph.ca.</Affiliation>
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<ForeName>Éva</ForeName>
<Initials>É</Initials>
<AffiliationInfo><Affiliation>Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2 W1, Canada. enagy@ovc.uoguelph.ca.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Centre for Public Health and Zoonoses, University of Guelph, Guelph, Canada. enagy@ovc.uoguelph.ca.</Affiliation>
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<Initials>G</Initials>
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<Author ValidYN="Y"><LastName>Weese</LastName>
<ForeName>Scott</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2 W1, Canada. jsweese@uoguelph.ca.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Centre for Public Health and Zoonoses, University of Guelph, Guelph, Canada. jsweese@uoguelph.ca.</Affiliation>
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<ForeName>Zvonimir</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2 W1, Canada. zpoljak@uoguelph.ca.</Affiliation>
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<AffiliationInfo><Affiliation>Centre for Public Health and Zoonoses, University of Guelph, Guelph, Canada. zpoljak@uoguelph.ca.</Affiliation>
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<name sortKey="Weese, Scott" sort="Weese, Scott" uniqKey="Weese S" first="Scott" last="Weese">Scott Weese</name>
<name sortKey="Wideman, Greg" sort="Wideman, Greg" uniqKey="Wideman G" first="Greg" last="Wideman">Greg Wideman</name>
</country>
</tree>
</affiliations>
</record>

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HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000278 | SxmlIndent | more

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{{Explor lien
   |wiki=    Sante
   |area=    GrippeCanadaV4
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:25416300
   |texte=   Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.
}}

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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:25416300" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a GrippeCanadaV4

This area was generated with Dilib version V0.6.35.
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	Serveur d'exploration sur la grippe au Canada
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Serveur d'exploration sur la grippe au Canada

Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.

Molecular characterization of H3N2 influenza A viruses isolated from Ontario swine in 2011 and 2012.

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