Replication of low pathogenic avian influenza virus in naturally infected Mallard ducks (Anas platyrhynchos) causes no morphologic lesions.
Identifieur interne : 000516 ( Main/Exploration ); précédent : 000515; suivant : 000517Replication of low pathogenic avian influenza virus in naturally infected Mallard ducks (Anas platyrhynchos) causes no morphologic lesions.
Auteurs : Pierre-Yves Daoust [Canada] ; Frederick S B. Kibenge ; Ron A M. Fouchier ; Marco W G. Van De Bildt ; Debby Van Riel ; Thijs KuikenSource :
- Journal of wildlife diseases [ 1943-3700 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Canards (virologie), Cloaque (virologie), Femelle (MeSH), Grippe chez les oiseaux (anatomopathologie), Grippe chez les oiseaux (épidémiologie), Ile-du-Prince-Édouard (épidémiologie), Immunohistochimie (médecine vétérinaire), Mâle (MeSH), RT-PCR (médecine vétérinaire), Réservoirs de maladies (médecine vétérinaire), Réservoirs de maladies (virologie), Virulence (MeSH), Virus de la grippe A (classification), Virus de la grippe A (isolement et purification), Virus de la grippe A (pathogénicité).
- MESH :
- anatomopathologie : Grippe chez les oiseaux.
- isolement et purification : Virus de la grippe A.
- médecine vétérinaire : Immunohistochimie, RT-PCR, Réservoirs de maladies.
- pathogénicité : Virus de la grippe A.
- virologie : Canards, Cloaque, Réservoirs de maladies.
- épidémiologie : Grippe chez les oiseaux, Ile-du-Prince-Édouard, Virus de la grippe A.
- Animaux, Femelle, Mâle, Virulence.
English descriptors
- KwdEn :
- Animals (MeSH), Cloaca (virology), Disease Reservoirs (veterinary), Disease Reservoirs (virology), Ducks (virology), Female (MeSH), Immunohistochemistry (veterinary), Influenza A virus (classification), Influenza A virus (isolation & purification), Influenza A virus (pathogenicity), Influenza in Birds (epidemiology), Influenza in Birds (pathology), Male (MeSH), Prince Edward Island (epidemiology), Reverse Transcriptase Polymerase Chain Reaction (veterinary), Virulence (MeSH).
- MESH :
- geographic , epidemiology : Prince Edward Island.
- classification : Influenza A virus.
- epidemiology : Influenza in Birds.
- isolation & purification : Influenza A virus.
- pathogenicity : Influenza A virus.
- pathology : Influenza in Birds.
- veterinary : Disease Reservoirs, Immunohistochemistry, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Cloaca, Disease Reservoirs, Ducks.
- Animals, Female, Male, Virulence.
Abstract
Although the Mallard (Anas platyrhynchos) is considered an important maintenance host for low pathogenic avian influenza (LPAI) viruses, viral cell tropism and pathology in naturally infected birds are largely unknown. In August 2006, we collected 19 free-living hatch-year Mallards that were positive for LPAI virus by real-time reverse-transcriptase polymerase chain reaction (RRT-PCR) in combined oropharyngeal and cloacal swabs. We investigated virus infection and associated lesions in the digestive and respiratory tracts by RRT-PCR, virus culture, immunohistochemistry (IHC), and histology. By RRT-PCR, 15 birds were positive in cloacal bursa, colon/cloaca, or both, and three were positive in lungs. Virus was isolated from eight birds and typed as H2N3 (three birds), H3N3 (two birds), H3N8 (one bird), H4N6 (one bird), and H?N3 (one bird). By IHC, birds were positive in the cloacal bursa (eight birds), colon (three), cecum (two), or ileum (one). Cell types infected were superficial epithelial cells of the bursa and epithelial cells of the intestinal villi and, less commonly, mucosal glands. By histology, there was no evidence of lesions associated with LPAI virus infection. These results show that epithelia of the cloacal bursa and of the lower intestine are important sites of natural LPAI virus infection in free-living hatch-year Mallards. The lack of lesions associated with this infection suggests that there is a strong selection by LPAI virus to cause minimal virulence in this maintenance host species.
DOI: 10.7589/0090-3558-47.2.401
PubMed: 21441193
Affiliations:
Links toward previous steps (curation, corpus...)
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Kibenge</name></author><author><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A M" last="Fouchier">Ron A M. Fouchier</name></author><author><name sortKey="Van De Bildt, Marco W G" sort="Van De Bildt, Marco W G" uniqKey="Van De Bildt M" first="Marco W G" last="Van De Bildt">Marco W G. Van De Bildt</name></author><author><name sortKey="Van Riel, Debby" sort="Van Riel, Debby" uniqKey="Van Riel D" first="Debby" last="Van Riel">Debby Van Riel</name></author><author><name sortKey="Kuiken, Thijs" sort="Kuiken, Thijs" uniqKey="Kuiken T" first="Thijs" last="Kuiken">Thijs Kuiken</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21441193</idno><idno type="pmid">21441193</idno><idno type="doi">10.7589/0090-3558-47.2.401</idno><idno type="wicri:Area/Main/Corpus">000550</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000550</idno><idno type="wicri:Area/Main/Curation">000550</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000550</idno><idno type="wicri:Area/Main/Exploration">000550</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Replication of low pathogenic avian influenza virus in naturally infected Mallard ducks (Anas platyrhynchos) causes no morphologic lesions.</title><author><name sortKey="Daoust, Pierre Yves" sort="Daoust, Pierre Yves" uniqKey="Daoust P" first="Pierre-Yves" last="Daoust">Pierre-Yves Daoust</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada. daoust@upei.ca</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3</wicri:regionArea><wicri:noRegion>PE C1A 4P3</wicri:noRegion></affiliation></author><author><name sortKey="Kibenge, Frederick S B" sort="Kibenge, Frederick S B" uniqKey="Kibenge F" first="Frederick S B" last="Kibenge">Frederick S B. Kibenge</name></author><author><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A M" last="Fouchier">Ron A M. Fouchier</name></author><author><name sortKey="Van De Bildt, Marco W G" sort="Van De Bildt, Marco W G" uniqKey="Van De Bildt M" first="Marco W G" last="Van De Bildt">Marco W G. Van De Bildt</name></author><author><name sortKey="Van Riel, Debby" sort="Van Riel, Debby" uniqKey="Van Riel D" first="Debby" last="Van Riel">Debby Van Riel</name></author><author><name sortKey="Kuiken, Thijs" sort="Kuiken, Thijs" uniqKey="Kuiken T" first="Thijs" last="Kuiken">Thijs Kuiken</name></author></analytic><series><title level="j">Journal of wildlife diseases</title><idno type="eISSN">1943-3700</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Cloaca (virology)</term><term>Disease Reservoirs (veterinary)</term><term>Disease Reservoirs (virology)</term><term>Ducks (virology)</term><term>Female (MeSH)</term><term>Immunohistochemistry (veterinary)</term><term>Influenza A virus (classification)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza A virus (pathogenicity)</term><term>Influenza in Birds (epidemiology)</term><term>Influenza in Birds (pathology)</term><term>Male (MeSH)</term><term>Prince Edward Island (epidemiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (veterinary)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Canards (virologie)</term><term>Cloaque (virologie)</term><term>Femelle (MeSH)</term><term>Grippe chez les oiseaux (anatomopathologie)</term><term>Grippe chez les oiseaux (épidémiologie)</term><term>Ile-du-Prince-Édouard (épidémiologie)</term><term>Immunohistochimie (médecine vétérinaire)</term><term>Mâle (MeSH)</term><term>RT-PCR (médecine vétérinaire)</term><term>Réservoirs de maladies (médecine vétérinaire)</term><term>Réservoirs de maladies (virologie)</term><term>Virulence (MeSH)</term><term>Virus de la grippe A (classification)</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus de la grippe A (pathogénicité)</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Prince Edward Island</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Grippe chez les oiseaux</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza in Birds</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Immunohistochimie</term><term>RT-PCR</term><term>Réservoirs de maladies</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Influenza in Birds</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Disease Reservoirs</term><term>Immunohistochemistry</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Canards</term><term>Cloaque</term><term>Réservoirs de maladies</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cloaca</term><term>Disease Reservoirs</term><term>Ducks</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe chez les oiseaux</term><term>Ile-du-Prince-Édouard</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Male</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Mâle</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although the Mallard (Anas platyrhynchos) is considered an important maintenance host for low pathogenic avian influenza (LPAI) viruses, viral cell tropism and pathology in naturally infected birds are largely unknown. In August 2006, we collected 19 free-living hatch-year Mallards that were positive for LPAI virus by real-time reverse-transcriptase polymerase chain reaction (RRT-PCR) in combined oropharyngeal and cloacal swabs. We investigated virus infection and associated lesions in the digestive and respiratory tracts by RRT-PCR, virus culture, immunohistochemistry (IHC), and histology. By RRT-PCR, 15 birds were positive in cloacal bursa, colon/cloaca, or both, and three were positive in lungs. Virus was isolated from eight birds and typed as H2N3 (three birds), H3N3 (two birds), H3N8 (one bird), H4N6 (one bird), and H?N3 (one bird). By IHC, birds were positive in the cloacal bursa (eight birds), colon (three), cecum (two), or ileum (one). Cell types infected were superficial epithelial cells of the bursa and epithelial cells of the intestinal villi and, less commonly, mucosal glands. By histology, there was no evidence of lesions associated with LPAI virus infection. These results show that epithelia of the cloacal bursa and of the lower intestine are important sites of natural LPAI virus infection in free-living hatch-year Mallards. The lack of lesions associated with this infection suggests that there is a strong selection by LPAI virus to cause minimal virulence in this maintenance host species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21441193</PMID><DateCompleted><Year>2011</Year><Month>05</Month><Day>16</Day></DateCompleted><DateRevised><Year>2012</Year><Month>05</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1943-3700</ISSN><JournalIssue CitedMedium="Internet"><Volume>47</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of wildlife diseases</Title><ISOAbbreviation>J. Wildl. Dis.</ISOAbbreviation></Journal><ArticleTitle>Replication of low pathogenic avian influenza virus in naturally infected Mallard ducks (Anas platyrhynchos) causes no morphologic lesions.</ArticleTitle><Pagination><MedlinePgn>401-9</MedlinePgn></Pagination><Abstract><AbstractText>Although the Mallard (Anas platyrhynchos) is considered an important maintenance host for low pathogenic avian influenza (LPAI) viruses, viral cell tropism and pathology in naturally infected birds are largely unknown. In August 2006, we collected 19 free-living hatch-year Mallards that were positive for LPAI virus by real-time reverse-transcriptase polymerase chain reaction (RRT-PCR) in combined oropharyngeal and cloacal swabs. We investigated virus infection and associated lesions in the digestive and respiratory tracts by RRT-PCR, virus culture, immunohistochemistry (IHC), and histology. By RRT-PCR, 15 birds were positive in cloacal bursa, colon/cloaca, or both, and three were positive in lungs. Virus was isolated from eight birds and typed as H2N3 (three birds), H3N3 (two birds), H3N8 (one bird), H4N6 (one bird), and H?N3 (one bird). By IHC, birds were positive in the cloacal bursa (eight birds), colon (three), cecum (two), or ileum (one). Cell types infected were superficial epithelial cells of the bursa and epithelial cells of the intestinal villi and, less commonly, mucosal glands. By histology, there was no evidence of lesions associated with LPAI virus infection. These results show that epithelia of the cloacal bursa and of the lower intestine are important sites of natural LPAI virus infection in free-living hatch-year Mallards. The lack of lesions associated with this infection suggests that there is a strong selection by LPAI virus to cause minimal virulence in this maintenance host species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Daoust</LastName><ForeName>Pierre-Yves</ForeName><Initials>PY</Initials><AffiliationInfo><Affiliation>Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PE C1A 4P3, Canada. daoust@upei.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kibenge</LastName><ForeName>Frederick S B</ForeName><Initials>FS</Initials></Author><Author ValidYN="Y"><LastName>Fouchier</LastName><ForeName>Ron A M</ForeName><Initials>RA</Initials></Author><Author ValidYN="Y"><LastName>van de Bildt</LastName><ForeName>Marco W G</ForeName><Initials>MW</Initials></Author><Author ValidYN="Y"><LastName>van Riel</LastName><ForeName>Debby</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Kuiken</LastName><ForeName>Thijs</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Wildl Dis</MedlineTA><NlmUniqueID>0244160</NlmUniqueID><ISSNLinking>0090-3558</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>J Wildl Dis. 2012 Apr;48(2):536</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002988" MajorTopicYN="N">Cloaca</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004197" MajorTopicYN="N">Disease Reservoirs</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004372" MajorTopicYN="N">Ducks</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005585" MajorTopicYN="N">Influenza in Birds</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011325" MajorTopicYN="N" Type="Geographic">Prince Edward Island</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>3</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21441193</ArticleId><ArticleId IdType="pii">47/2/401</ArticleId><ArticleId IdType="doi">10.7589/0090-3558-47.2.401</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Fouchier, Ron A M" sort="Fouchier, Ron A M" uniqKey="Fouchier R" first="Ron A M" last="Fouchier">Ron A M. Fouchier</name><name sortKey="Kibenge, Frederick S B" sort="Kibenge, Frederick S B" uniqKey="Kibenge F" first="Frederick S B" last="Kibenge">Frederick S B. Kibenge</name><name sortKey="Kuiken, Thijs" sort="Kuiken, Thijs" uniqKey="Kuiken T" first="Thijs" last="Kuiken">Thijs Kuiken</name><name sortKey="Van De Bildt, Marco W G" sort="Van De Bildt, Marco W G" uniqKey="Van De Bildt M" first="Marco W G" last="Van De Bildt">Marco W G. Van De Bildt</name><name sortKey="Van Riel, Debby" sort="Van Riel, Debby" uniqKey="Van Riel D" first="Debby" last="Van Riel">Debby Van Riel</name></noCountry><country name="Canada"><noRegion><name sortKey="Daoust, Pierre Yves" sort="Daoust, Pierre Yves" uniqKey="Daoust P" first="Pierre-Yves" last="Daoust">Pierre-Yves Daoust</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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