Nationwide molecular surveillance of pandemic H1N1 influenza A virus genomes: Canada, 2009.
Identifieur interne : 000535 ( Main/Exploration ); précédent : 000534; suivant : 000536Nationwide molecular surveillance of pandemic H1N1 influenza A virus genomes: Canada, 2009.
Auteurs : Morag Graham [Canada] ; Binhua Liang ; Gary Van Domselaar ; Nathalie Bastien ; Carole Beaudoin ; Shaun Tyler ; Brynn Kaplen ; Erika Landry ; Yan LiSource :
- PloS one [ 1932-6203 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Sous-type H1N1 du virus de la grippe A.
- épidémiologie : Canada, Grippe humaine.
- Données de séquences moléculaires, Génome viral, Humains, Hétérozygote, Mutation, Pandémies, Surveillance de la population.
- Wicri :
- geographic : Canada.
English descriptors
- KwdEn :
- MESH :
- geographic , epidemiology : Canada.
- epidemiology : Influenza, Human.
- genetics : Influenza A Virus, H1N1 Subtype.
- Genome, Viral, Heterozygote, Humans, Molecular Sequence Data, Mutation, Pandemics, Population Surveillance.
Abstract
BACKGROUND
In April 2009, a novel triple-reassortant swine influenza A H1N1 virus ("A/H1N1pdm"; also known as SOIV) was detected and spread globally as the first influenza pandemic of the 21(st) century. Sequencing has since been conducted at an unprecedented rate globally in order to monitor the diversification of this emergent virus and to track mutations that may affect virus behavior.
METHODOLOGY/PRINCIPAL FINDINGS
By Sanger sequencing, we determined consensus whole-genome sequences for A/H1N1pdm viruses sampled nationwide in Canada over 33 weeks during the 2009 first and second pandemic waves. A total of 235 virus genomes sampled from unique subjects were analyzed, providing insight into the temporal and spatial trajectory of A/H1N1pdm lineages within Canada. Three clades (2, 3, and 7) were identifiable within the first two weeks of A/H1N1pdm appearance, with clades 5 and 6 appearing thereafter; further diversification was not apparent. Only two viral sites displayed evidence of adaptive evolution, located in hemagglutinin (HA) corresponding to D222 in the HA receptor-binding site, and to E374 at HA2-subunit position 47. Among the Canadian sampled viruses, we observed notable genetic diversity (1.47 x 10⁻³ amino acid substitutions per site) in the gene encoding PB1, particularly within the viral genomic RNA (vRNA)-binding domain (residues 493-757). This genome data set supports the conclusion that A/H1N1pdm is evolving but not excessively relative to other H1N1 influenza A viruses. Entropy analysis was used to investigate whether any mutated A/H1N1pdm protein residues were associated with infection severity; however no virus genotypes were observed to trend with infection severity. One virus that harboured heterozygote coding mutations, including PB2 D567D/G, was attributed to a severe and potentially mixed infection; yet the functional significance of this PB2 mutation remains unknown.
CONCLUSIONS/SIGNIFICANCE
These findings contribute to enhanced understanding of Influenza A/H1N1pdm viral dynamics.
DOI: 10.1371/journal.pone.0016087
PubMed: 21249207
PubMed Central: PMC3017559
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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xml:lang="fr"><term>Canada (épidémiologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Grippe humaine (épidémiologie)</term><term>Génome viral (MeSH)</term><term>Humains (MeSH)</term><term>Hétérozygote (MeSH)</term><term>Mutation (MeSH)</term><term>Pandémies (MeSH)</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Surveillance de la population (MeSH)</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Canada</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Canada</term><term>Grippe humaine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genome, Viral</term><term>Heterozygote</term><term>Humans</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Pandemics</term><term>Population Surveillance</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Humains</term><term>Hétérozygote</term><term>Mutation</term><term>Pandémies</term><term>Surveillance de la population</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Canada</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>In April 2009, a novel triple-reassortant swine influenza A H1N1 virus ("A/H1N1pdm"; also known as SOIV) was detected and spread globally as the first influenza pandemic of the 21(st) century. Sequencing has since been conducted at an unprecedented rate globally in order to monitor the diversification of this emergent virus and to track mutations that may affect virus behavior.</p></div><div type="abstract" xml:lang="en"><p><b>METHODOLOGY/PRINCIPAL FINDINGS</b></p><p>By Sanger sequencing, we determined consensus whole-genome sequences for A/H1N1pdm viruses sampled nationwide in Canada over 33 weeks during the 2009 first and second pandemic waves. A total of 235 virus genomes sampled from unique subjects were analyzed, providing insight into the temporal and spatial trajectory of A/H1N1pdm lineages within Canada. Three clades (2, 3, and 7) were identifiable within the first two weeks of A/H1N1pdm appearance, with clades 5 and 6 appearing thereafter; further diversification was not apparent. Only two viral sites displayed evidence of adaptive evolution, located in hemagglutinin (HA) corresponding to D222 in the HA receptor-binding site, and to E374 at HA2-subunit position 47. Among the Canadian sampled viruses, we observed notable genetic diversity (1.47 x 10⁻³ amino acid substitutions per site) in the gene encoding PB1, particularly within the viral genomic RNA (vRNA)-binding domain (residues 493-757). This genome data set supports the conclusion that A/H1N1pdm is evolving but not excessively relative to other H1N1 influenza A viruses. Entropy analysis was used to investigate whether any mutated A/H1N1pdm protein residues were associated with infection severity; however no virus genotypes were observed to trend with infection severity. One virus that harboured heterozygote coding mutations, including PB2 D567D/G, was attributed to a severe and potentially mixed infection; yet the functional significance of this PB2 mutation remains unknown.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS/SIGNIFICANCE</b></p><p>These findings contribute to enhanced understanding of Influenza A/H1N1pdm viral dynamics.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21249207</PMID><DateCompleted><Year>2011</Year><Month>08</Month><Day>02</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>05</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Jan</Month><Day>07</Day></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS ONE</ISOAbbreviation></Journal><ArticleTitle>Nationwide molecular surveillance of pandemic H1N1 influenza A virus genomes: Canada, 2009.</ArticleTitle><Pagination><MedlinePgn>e16087</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0016087</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">In April 2009, a novel triple-reassortant swine influenza A H1N1 virus ("A/H1N1pdm"; also known as SOIV) was detected and spread globally as the first influenza pandemic of the 21(st) century. Sequencing has since been conducted at an unprecedented rate globally in order to monitor the diversification of this emergent virus and to track mutations that may affect virus behavior.</AbstractText><AbstractText Label="METHODOLOGY/PRINCIPAL FINDINGS" NlmCategory="RESULTS">By Sanger sequencing, we determined consensus whole-genome sequences for A/H1N1pdm viruses sampled nationwide in Canada over 33 weeks during the 2009 first and second pandemic waves. A total of 235 virus genomes sampled from unique subjects were analyzed, providing insight into the temporal and spatial trajectory of A/H1N1pdm lineages within Canada. Three clades (2, 3, and 7) were identifiable within the first two weeks of A/H1N1pdm appearance, with clades 5 and 6 appearing thereafter; further diversification was not apparent. Only two viral sites displayed evidence of adaptive evolution, located in hemagglutinin (HA) corresponding to D222 in the HA receptor-binding site, and to E374 at HA2-subunit position 47. Among the Canadian sampled viruses, we observed notable genetic diversity (1.47 x 10⁻³ amino acid substitutions per site) in the gene encoding PB1, particularly within the viral genomic RNA (vRNA)-binding domain (residues 493-757). This genome data set supports the conclusion that A/H1N1pdm is evolving but not excessively relative to other H1N1 influenza A viruses. Entropy analysis was used to investigate whether any mutated A/H1N1pdm protein residues were associated with infection severity; however no virus genotypes were observed to trend with infection severity. One virus that harboured heterozygote coding mutations, including PB2 D567D/G, was attributed to a severe and potentially mixed infection; yet the functional significance of this PB2 mutation remains unknown.</AbstractText><AbstractText Label="CONCLUSIONS/SIGNIFICANCE" NlmCategory="CONCLUSIONS">These findings contribute to enhanced understanding of Influenza A/H1N1pdm viral dynamics.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Graham</LastName><ForeName>Morag</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada. morag.graham@phac-aspc.gc.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Binhua</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Van Domselaar</LastName><ForeName>Gary</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Bastien</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Beaudoin</LastName><ForeName>Carole</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Tyler</LastName><ForeName>Shaun</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Kaplen</LastName><ForeName>Brynn</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Landry</LastName><ForeName>Erika</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><CollectiveName>National Influenza A/H1N1pdm Genomics Study Team (NIGST)</CollectiveName></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>01</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002170" MajorTopicYN="N" Type="Geographic">Canada</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="Y">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006579" MajorTopicYN="N">Heterozygote</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058873" MajorTopicYN="Y">Pandemics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011159" MajorTopicYN="Y">Population Surveillance</DescriptorName></MeshHeading></MeshHeadingList><InvestigatorList><Investigator ValidYN="Y"><LastName>Kumar</LastName><ForeName>Anand</ForeName><Initials>A</Initials></Investigator><Investigator ValidYN="Y"><LastName>Drews</LastName><ForeName>Steven</ForeName><Initials>S</Initials></Investigator><Investigator ValidYN="Y"><LastName>Fonseca</LastName><ForeName>Kevin</ForeName><Initials>K</Initials></Investigator><Investigator ValidYN="Y"><LastName>Steppan</LastName><ForeName>Pamela</ForeName><Initials>P</Initials></Investigator><Investigator ValidYN="Y"><LastName>Van Caeseele</LastName><ForeName>Paul</ForeName><Initials>P</Initials></Investigator><Investigator ValidYN="Y"><LastName>Cole</LastName><ForeName>Roy</ForeName><Initials>R</Initials></Investigator><Investigator ValidYN="Y"><LastName>Garceau</LastName><ForeName>Richard</ForeName><Initials>R</Initials></Investigator><Investigator ValidYN="Y"><LastName>Savoie</LastName><ForeName>Suzanne</ForeName><Initials>S</Initials></Investigator><Investigator ValidYN="Y"><LastName>Butt</LastName><ForeName>Kelly</ForeName><Initials>K</Initials></Investigator><Investigator ValidYN="Y"><LastName>Stead</LastName><ForeName>Florence</ForeName><Initials>F</Initials></Investigator><Investigator ValidYN="Y"><LastName>Hatchette</LastName><ForeName>Todd F</ForeName><Initials>TF</Initials></Investigator><Investigator ValidYN="Y"><LastName>Charest</LastName><ForeName>Hugues</ForeName><Initials>H</Initials></Investigator><Investigator ValidYN="Y"><LastName>Bangura</LastName><ForeName>Helen</ForeName><Initials>H</Initials></Investigator></InvestigatorList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>10</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>12</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>1</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>8</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21249207</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0016087</ArticleId><ArticleId IdType="pmc">PMC3017559</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Antiviral Res. 2008 Sep;79(3):166-78</Citation><ArticleIdList><ArticleId IdType="pubmed">18547656</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20365-70</Citation><ArticleIdList><ArticleId IdType="pubmed">19918065</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2005 May;22(5):1208-22</Citation><ArticleIdList><ArticleId IdType="pubmed">15703242</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2002 May 10;85(2):199-210</Citation><ArticleIdList><ArticleId IdType="pubmed">12034486</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 2008;62:403-24</Citation><ArticleIdList><ArticleId IdType="pubmed">18785841</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Jul 10;325(5937):197-201</Citation><ArticleIdList><ArticleId IdType="pubmed">19465683</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 2009 Nov 4;302(17):1872-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19822627</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2009 Nov 17;4(11):e7789</Citation><ArticleIdList><ArticleId IdType="pubmed">19924230</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Rev. 1992 Mar;56(1):152-79</Citation><ArticleIdList><ArticleId IdType="pubmed">1579108</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2009 Sep;83(17):8832-41</Citation><ArticleIdList><ArticleId IdType="pubmed">19553313</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2005 Dec;79(23):14933-44</Citation><ArticleIdList><ArticleId IdType="pubmed">16282492</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Jun 25;459(7250):1122-5</Citation><ArticleIdList><ArticleId IdType="pubmed">19516283</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2007 Feb 2;315(5812):655-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17272724</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2009 May 21;4(5):e5658</Citation><ArticleIdList><ArticleId IdType="pubmed">19462010</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2010 Jun 15;26(12):1569-71</Citation><ArticleIdList><ArticleId IdType="pubmed">20421198</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010 Jul 22;6(7):e1001015</Citation><ArticleIdList><ArticleId IdType="pubmed">20661429</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2006 Dec;2(12):e125</Citation><ArticleIdList><ArticleId IdType="pubmed">17140286</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2010 Sep 30;405(2):314-21</Citation><ArticleIdList><ArticleId IdType="pubmed">20598336</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2007 Nov 1;23(21):2947-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17846036</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2010 Aug;151(2):192-9</Citation><ArticleIdList><ArticleId IdType="pubmed">20493216</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Sep;84(17):8691-9</Citation><ArticleIdList><ArticleId IdType="pubmed">20573828</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Curr. 2010 Mar 22;2:RRN1152</Citation><ArticleIdList><ArticleId IdType="pubmed">20352039</ArticleId></ArticleIdList></Reference><Reference><Citation>Vet Res. 2010 Nov-Dec;41(6):38</Citation><ArticleIdList><ArticleId IdType="pubmed">20167200</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Curr. 2010 Jun 01;2:RRN1162</Citation><ArticleIdList><ArticleId IdType="pubmed">20535229</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2000 Jul;74(14):6538-45</Citation><ArticleIdList><ArticleId IdType="pubmed">10864667</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2008 May 29;453(7195):615-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18418375</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2009 Apr 10;324(5924):246-51</Citation><ArticleIdList><ArticleId IdType="pubmed">19251591</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2010 Jan 01;5(1):e8553</Citation><ArticleIdList><ArticleId IdType="pubmed">20049332</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2001 Mar 1;281(1):102-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11222100</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Comput Biol. 2007 Nov;3(11):e231</Citation><ArticleIdList><ArticleId IdType="pubmed">18039027</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Curr. 2009 Nov 03;1:RRN1126</Citation><ArticleIdList><ArticleId IdType="pubmed">20029664</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Apr;80(7):3675-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16537638</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2002 Sep;76(17):8757-68</Citation><ArticleIdList><ArticleId IdType="pubmed">12163596</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2007 Dec 05;4:134</Citation><ArticleIdList><ArticleId IdType="pubmed">18053252</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Cycle. 2010 Mar 1;9(5):958-70</Citation><ArticleIdList><ArticleId IdType="pubmed">20160492</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Biochim Biophys Sin (Shanghai). 2007 Mar;39(3):155-62</Citation><ArticleIdList><ArticleId IdType="pubmed">17342253</ArticleId></ArticleIdList></Reference><Reference><Citation>Glycobiology. 1992 Jun;2(3):233-40</Citation><ArticleIdList><ArticleId IdType="pubmed">1379858</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2010 Mar 15;5(3):e9693</Citation><ArticleIdList><ArticleId IdType="pubmed">20300625</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2006 Feb 3;355(5):1143-55</Citation><ArticleIdList><ArticleId IdType="pubmed">16343533</ArticleId></ArticleIdList></Reference><Reference><Citation>Euro Surveill. 2010 Mar 04;15(9):</Citation><ArticleIdList><ArticleId IdType="pubmed">20214869</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2008 Feb 8;4(2):e11</Citation><ArticleIdList><ArticleId IdType="pubmed">18248089</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2001 Dec;146(12):2275-89</Citation><ArticleIdList><ArticleId IdType="pubmed">11811679</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Cell Biol. 2010 Aug;88(4):589-93</Citation><ArticleIdList><ArticleId IdType="pubmed">20651829</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2009 May 1;25(9):1189-91</Citation><ArticleIdList><ArticleId IdType="pubmed">19151095</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21312-6</Citation><ArticleIdList><ArticleId IdType="pubmed">19995968</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Struct Biol. 2010 Feb;20(1):104-13</Citation><ArticleIdList><ArticleId IdType="pubmed">20061134</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2010 Feb 03;5(2):e9025</Citation><ArticleIdList><ArticleId IdType="pubmed">20140252</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2009 May 29;6:67</Citation><ArticleIdList><ArticleId IdType="pubmed">19476650</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2008 Aug 29;4(8):e1000136</Citation><ArticleIdList><ArticleId IdType="pubmed">18769709</ArticleId></ArticleIdList></Reference><Reference><Citation>CMAJ. 2009 Aug 4;181(3-4):159-63</Citation><ArticleIdList><ArticleId IdType="pubmed">19620268</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2010 May 6;362(18):1708-19</Citation><ArticleIdList><ArticleId IdType="pubmed">20445182</ArticleId></ArticleIdList></Reference><Reference><Citation>Heredity (Edinb). 2011 Feb;106(2):383-90</Citation><ArticleIdList><ArticleId IdType="pubmed">20551981</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Struct Mol Biol. 2010 May;17(5):530-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20383144</ArticleId></ArticleIdList></Reference><Reference><Citation>Brief Bioinform. 2008 Jul;9(4):299-306</Citation><ArticleIdList><ArticleId IdType="pubmed">18417537</ArticleId></ArticleIdList></Reference><Reference><Citation>Biol Direct. 2009 May 20;4:18; discussion 18</Citation><ArticleIdList><ArticleId IdType="pubmed">19457254</ArticleId></ArticleIdList></Reference><Reference><Citation>Comput Appl Biosci. 1996 Aug;12(4):357-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8902363</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Bastien, Nathalie" sort="Bastien, Nathalie" uniqKey="Bastien N" first="Nathalie" last="Bastien">Nathalie Bastien</name><name sortKey="Beaudoin, Carole" 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